- Email: [email protected]
Expression of sialyl Lewisx on epidermal Langerhans cells in the human skin
16
Poster Presentations
375
378
LAR(lBwwIG* CELLS IR CORTACT RYPERSEREXTIVITY TO PLATIAOID MEZTALL5AKD ATOPIC Dk3GLATITIS:Victor I. Prokhorenkov, Department of Dermatology and Venemlogy, Krasnoyarek Medical Institute. Krasnovarsk. Russia Langerhans' ceils (LC)-perfirming the antigen-producing function in epidemocyte play an important part in pathogenesis of contact eczama and atopic dermatitis. There is an interest in oompawtive study LC function in contact allergy and atopio dermrtitie. We studied skin microbioptats in 12 workera with contact eceema induced by induetrial contact with PlatinOidB (etiologic diargmsie confirmed by the skin tests) and in 7 patients with atopic dermatitis. In ohilled aectione Langerhans' cells were determined by immunonorphologic method usln antibodies and Ia-, T&AC) and alao by Y.B. Shelley, L.&U (1977) and B.Sjllberg (1978) methods. There wee revealed the decreaee of RLA-DR quantity of LC in epidemia in patiente with contact eczema. Intracellular contain of catecholanlne~ "88 not changed reliably. In atopic dermatitis RLA-DR quantity of Lo decreased only in acute dermatosls especially in patients who received steroid ointments loaally end ultraviolet irradiation. Slmultaneouely in patients with atopic dematitie there wee revealed a marked increase of the monocytea quantity which had HLA-DR moleoulee on their eurface membranea up to 82,221( in control group 64.8%).
CHARAC,B?,ZA’“ON OF A REVERStBLE tNTERMEDtA’t!J FORMED DURING THE SPONTANEO”S ,N.4cr,“AT,ON OF ““MAN 9x-4 TRYPTASE &ac.c Y. -Darrell R. McCaslin. and Nocmman M. Sag Pennsylvania, Philadelphia, PA,Rutgers University. Newark. NJ, USA Regulation of the human mast cell wine proteinas% n’yprasc. in inflammation is unclear because there is no physiological inhibitor to inactivate the secreted enzyme. The isolated proteinase rapidly inactivates when incubatedin solutions of physiologwal salt and pH, suggesting that an auto-inactivation process may replace the need for a specific inhibitor. We have provided evidencethat loss of activity involves the rapid equilibmtion of active ayptase with an inactive form capableof re-activationby heptin which then slowly decays to a permanently inacwated state. This study further characterizes the prop&es of this “inrennediate”. Comparison of the secondary suucwre of active and inactivated tryptase by circulardicrohism (CD) demonsmateda relatively small changem the CD spectrum after inactivation. Most notable was the disappearanceof a negativeabsorption peakcenteredat approximately230 nm in active tryptax. When monitored as a funcrion of time, absorbanceat 230 nm decreasedat a rate comparableto activity loss, suggestingthat the changein the CD spectmm could be associated with formation of the reversible intermediate. The specifiaty of the reacrivation process for heparin also was analyzed. About 50% maximal recovery of activity occured at a heparinconcentrationappoximatelyequivalentto inactive tryptase. Implying that binding was necessary for reactivation. Similar results were obtained with dextran sulfate, a highly sulfated polymer of glucose Less recovery was achieved with glycosaminoglycans less sulfaaredthan heparin and no reactivanon was achieved with unsulfared dcxtran. These studies indicate that tryptaseinacwarion involves a relatively small confonnarional change,consistent with the formation of a reversible mrermediate. Reactivation of this intermediate appearsto be a non-specific process involving binding of negatively chargedpolymen by clamstatic interactions.
376
379
AETINOID-COMBINATION THERAPY FOR PSORIASIS DOWNREGULATES INCREASED EXPRESSION OF CELL ADHESION MOLECULE BY ENDOTHELIAL CELLS F(llchiro DannQ, Department of Dermatoloav. Shioa Univeraitv of Medical Science. Otsu. Japan. Various treatment modalittes with different therapeutic mkhanisms are beneficial for treatment of psoriasis. Although immunomodulalory effects of them have recently been emphawed as an important aspecl of action “I.
mechanisms, effects on the cell adhesion molecule (CAM) expressmn by endothelial cells (ECs.) have not yet been thoroughly investigated. In this studv. biopsv wecimens were taken from tvpical lesions of 23 cases before and’buriig ‘trdatment with psoralen plu;’ UVA (PUVA) radiation, UVB radiation, and oral eicosapentaenoic acid (EPA) alone or combined with oral etretinate (Re). lmmunohistochemical techniques were applied to visualize the expression of ELAM-1 and ICAMby ECs. The staining intensltv of them was correlated with the histological features to determine whether alterations in the CAM expression precede or follow the normalization of the histological characteristics. Following F&-combination therapies, increased expression of both CAMS by ECs in the papillary dermis was remarkably decreased to the baseline conditions before the histological features were normalized. while a delayed or unremarkable response was observed to PUVA. UVB, and EPA alone. The preliminary results suggest that whether Re alone can directly or indirectly downregulate the immunologic function of activated EC% which in part plays a pathogenetic role in psoriasis, or it enhances the inhibitory effect of other single regimens on ECs.
380
377 EI..lMINATION OF
IMMUNE [email protected] Flora Ba~?wwn, lnstltutr
Beletskaya, and Art,t'ltlal Orears.
BY
h!oscow.Russia
THE SKIN. for Transyla-
Ludnnla
EXPRESSION LANGERHANS
OF
SIALYL
LEWISX
ON
EPIDERMAL
CELLS IN THE HUMAN SKIN. Keiii Ohta. Mavumi Fuiita. Yuii Horiauchi. Fukumi Furukawa and Sadao Imamura. Department of Dermatology, Kyoto University Faculty of Medicine, Kyoto, Japan A carbohydrate antigen sialyl-Lewisx(s-Lex) is well known as a tumor-associated antigen. Recently, it has been demonstrated that the s-Lex molecule is also present on leukocyte and serves in the process of inflammation as a ligand for endothelial-leukocyte adhesion molecule-l which is expressed in the activated endothelial cells of blood vessel walls. We examined the expression of s-Lex in the human skin by immunohistochemical staining. Cryostat sections were stained with the avidin-biotin-peroxidase complex g~o,ce;~r,e;idAfE, OS;.:;; positive dendritic cells ware observe human skin. To make clear whether these cells are Langerhans cells or not, we used anti-CDla antibody and monoclonal Lag antibody that specifically reacts with Birbeck granules and related structures of human Langerhans cells. Double imunofluorescence studies revealed that s-Lex positive dendritic cells in the epidermis were CD1 a-positive and Lag-positive. These results suggest that sLex epidermal dendritic cells are Lan erhans cells and s-Lex may be a new and useful marker of Langer II ans cells.
Poster Presentations
375
378
LAR(lBwwIG* CELLS IR CORTACT RYPERSEREXTIVITY TO PLATIAOID MEZTALL5AKD ATOPIC Dk3GLATITIS:Victor I. Prokhorenkov, Department of Dermatology and Venemlogy, Krasnoyarek Medical Institute. Krasnovarsk. Russia Langerhans' ceils (LC)-perfirming the antigen-producing function in epidemocyte play an important part in pathogenesis of contact eczama and atopic dermatitis. There is an interest in oompawtive study LC function in contact allergy and atopio dermrtitie. We studied skin microbioptats in 12 workera with contact eceema induced by induetrial contact with PlatinOidB (etiologic diargmsie confirmed by the skin tests) and in 7 patients with atopic dermatitis. In ohilled aectione Langerhans' cells were determined by immunonorphologic method usln antibodies and Ia-, T&AC) and alao by Y.B. Shelley, L.&U (1977) and B.Sjllberg (1978) methods. There wee revealed the decreaee of RLA-DR quantity of LC in epidemia in patiente with contact eczema. Intracellular contain of catecholanlne~ "88 not changed reliably. In atopic dermatitis RLA-DR quantity of Lo decreased only in acute dermatosls especially in patients who received steroid ointments loaally end ultraviolet irradiation. Slmultaneouely in patients with atopic dematitie there wee revealed a marked increase of the monocytea quantity which had HLA-DR moleoulee on their eurface membranea up to 82,221( in control group 64.8%).
CHARAC,B?,ZA’“ON OF A REVERStBLE tNTERMEDtA’t!J FORMED DURING THE SPONTANEO”S ,N.4cr,“AT,ON OF ““MAN 9x-4 TRYPTASE &ac.c Y. -Darrell R. McCaslin. and Nocmman M. Sag Pennsylvania, Philadelphia, PA,Rutgers University. Newark. NJ, USA Regulation of the human mast cell wine proteinas% n’yprasc. in inflammation is unclear because there is no physiological inhibitor to inactivate the secreted enzyme. The isolated proteinase rapidly inactivates when incubatedin solutions of physiologwal salt and pH, suggesting that an auto-inactivation process may replace the need for a specific inhibitor. We have provided evidencethat loss of activity involves the rapid equilibmtion of active ayptase with an inactive form capableof re-activationby heptin which then slowly decays to a permanently inacwated state. This study further characterizes the prop&es of this “inrennediate”. Comparison of the secondary suucwre of active and inactivated tryptase by circulardicrohism (CD) demonsmateda relatively small changem the CD spectrum after inactivation. Most notable was the disappearanceof a negativeabsorption peakcenteredat approximately230 nm in active tryptax. When monitored as a funcrion of time, absorbanceat 230 nm decreasedat a rate comparableto activity loss, suggestingthat the changein the CD spectmm could be associated with formation of the reversible intermediate. The specifiaty of the reacrivation process for heparin also was analyzed. About 50% maximal recovery of activity occured at a heparinconcentrationappoximatelyequivalentto inactive tryptase. Implying that binding was necessary for reactivation. Similar results were obtained with dextran sulfate, a highly sulfated polymer of glucose Less recovery was achieved with glycosaminoglycans less sulfaaredthan heparin and no reactivanon was achieved with unsulfared dcxtran. These studies indicate that tryptaseinacwarion involves a relatively small confonnarional change,consistent with the formation of a reversible mrermediate. Reactivation of this intermediate appearsto be a non-specific process involving binding of negatively chargedpolymen by clamstatic interactions.
376
379
AETINOID-COMBINATION THERAPY FOR PSORIASIS DOWNREGULATES INCREASED EXPRESSION OF CELL ADHESION MOLECULE BY ENDOTHELIAL CELLS F(llchiro DannQ, Department of Dermatoloav. Shioa Univeraitv of Medical Science. Otsu. Japan. Various treatment modalittes with different therapeutic mkhanisms are beneficial for treatment of psoriasis. Although immunomodulalory effects of them have recently been emphawed as an important aspecl of action “I.
mechanisms, effects on the cell adhesion molecule (CAM) expressmn by endothelial cells (ECs.) have not yet been thoroughly investigated. In this studv. biopsv wecimens were taken from tvpical lesions of 23 cases before and’buriig ‘trdatment with psoralen plu;’ UVA (PUVA) radiation, UVB radiation, and oral eicosapentaenoic acid (EPA) alone or combined with oral etretinate (Re). lmmunohistochemical techniques were applied to visualize the expression of ELAM-1 and ICAMby ECs. The staining intensltv of them was correlated with the histological features to determine whether alterations in the CAM expression precede or follow the normalization of the histological characteristics. Following F&-combination therapies, increased expression of both CAMS by ECs in the papillary dermis was remarkably decreased to the baseline conditions before the histological features were normalized. while a delayed or unremarkable response was observed to PUVA. UVB, and EPA alone. The preliminary results suggest that whether Re alone can directly or indirectly downregulate the immunologic function of activated EC% which in part plays a pathogenetic role in psoriasis, or it enhances the inhibitory effect of other single regimens on ECs.
380
377 EI..lMINATION OF
IMMUNE [email protected] Flora Ba~?wwn, lnstltutr
Beletskaya, and Art,t'ltlal Orears.
BY
h!oscow.Russia
THE SKIN. for Transyla-
Ludnnla
EXPRESSION LANGERHANS
OF
SIALYL
LEWISX
ON
EPIDERMAL
CELLS IN THE HUMAN SKIN. Keiii Ohta. Mavumi Fuiita. Yuii Horiauchi. Fukumi Furukawa and Sadao Imamura. Department of Dermatology, Kyoto University Faculty of Medicine, Kyoto, Japan A carbohydrate antigen sialyl-Lewisx(s-Lex) is well known as a tumor-associated antigen. Recently, it has been demonstrated that the s-Lex molecule is also present on leukocyte and serves in the process of inflammation as a ligand for endothelial-leukocyte adhesion molecule-l which is expressed in the activated endothelial cells of blood vessel walls. We examined the expression of s-Lex in the human skin by immunohistochemical staining. Cryostat sections were stained with the avidin-biotin-peroxidase complex g~o,ce;~r,e;idAfE, OS;.:;; positive dendritic cells ware observe human skin. To make clear whether these cells are Langerhans cells or not, we used anti-CDla antibody and monoclonal Lag antibody that specifically reacts with Birbeck granules and related structures of human Langerhans cells. Double imunofluorescence studies revealed that s-Lex positive dendritic cells in the epidermis were CD1 a-positive and Lag-positive. These results suggest that sLex epidermal dendritic cells are Lan erhans cells and s-Lex may be a new and useful marker of Langer II ans cells.