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EXPRESSION OF TETRASPANINS CD9 AND CD63 IN HEAD AND NECK SQUAMOUS CELL CARCINOMA: AN IMMUNOHISTOCHEMICAL STUDY
ABSTRACTS
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Results: The IC50 of HBEE for MCF-7 tumor cells (36.28 mg/mL) was significantly higher than for MCF-10 A nontumor cells (145.13 mg/mL). In vivo assay demonstrated that HBEE at 30 mg/Kg significantly reduced tumor weight (P < .05) and Ki-67 expression (P < .01), and increased apoptosis index (P < .001) and leukocyte count in peripheral blood (P < .05) in comparison with CTR. Conclusions: These data suggest that the HBEE exerted selective cytotoxic for tumor cells and antitumor activity comparable to 5-FU.
EXPRESSION OF TETRASPANINS CD9 AND CD63 IN HEAD AND NECK SQUAMOUS CELL CARCINOMA: AN IMMUNOHISTOCHEMICAL STUDY. RODRIGO NEVES-SILVA, ALFREDO RIBEIRO SILVA, ANDRESSA DUARTE, SUZIENE CAROLINE SILVA, LUCIANA YAMAMOTO ALMEIDA, CARLA BENTO NELEM COLTURATO and, JORGE ESQUICHE LEON Objective: Tetraspanins are proteins involved in several cellular processes, including association with tumor progression. In this study, the immunoexpression of tetraspanins in head and neck squamous cell carcinoma (HNSCC) were assessed, aiming to evaluate the participation of these molecules in carcinogenesis. Study Design: An immunohistochemical study was carried out using the tissue microarray technique to evaluate the tetraspanins CD9 and CD63. One hundred and twenty-one HNSCCs were analyzed (male, n = 97; female, n = 24; mean age, 60.4 years). The anatomic sites were 84 oral cavity, 23 oropharynx, 3 skin, 7 paranasal sinuses, 2 larynx, 1 nasopharynx, and 1 pharynx. Results: Only 103 HNSCCs were available for analysis. For CD9, 69 (67%) HNSCCs were positive (46 oral cavity, 17 oropharynx, 5 paranasal sinuses, and 1 skin). For CD63, 11 (10.7%) HNSCCs were positive (7 oral cavity, 2 oropharynx, 1 paranasal sinuses, and 1 skin). Conclusions: Our results suggest participation of tetraspanins CD9 and CD63 in the HNSCC pathogenesis. Further studies are necessary to determinate their prognostic effect with therapeutic implications. FAPESP grant 2016/11419-0.
DIAGNOSTIC VALUE OF MICRO RNA BIOMARKERS AS AN EARLY DIAGNOSTIC METHOD OF ORAL SQUAMOUS CELL CARCINOMA. CRISTIAN MAURICIO ROJAS and, MATHIAS MAURICIO PARRA APABLAZA Background: To date, biopsy is the gold standard for diagnosis of oral squamous cell carcinoma (OSCC). Due to its limitations and late diagnosis of OSCC, it’s necessary to develop new techniques that allow an early diagnosis of oral cancer. Recent studies indicate that micro RNAs biomarkers (miRNAs) present in body fluids such as blood, plasma, serum, and saliva offer an effective method for the early diagnosis of OSCC. Objective: To describe the diagnostic value of miRNAs biomarkers in body fluids as a method of early diagnosis of OSCC. Method: An electronic search was carried out in the following databases: Pubmed, Trip Database, Epistemonikos, Cochrane Library, Scopus, Ebsco, and Bireme. From the selected studies, a retrograde search was performed. Results: A total of 24 articles, published between 2008 and 2017, met the selection criteria and were included in this
OOOO January 2020 review. The studies support the use of miRNAs biomarkers as a potential tool to obtain an early diagnosis of OSCC. Conclusions: According to the studies analyzed in this review, miRNAs biomarkers in body fluids have an undoubted potential, although their diagnostic value should be considered moderate until new studies demonstrate and validate their use as an early diagnosis method of OSCC.
NEUROENDOCRINE DIFFERENTIATION AND CELL PROLIFERATION IN ORAL AND OROPHARYNGEAL SQUAMOUS CELL CARCINOMA. CARLA BENTO NELEM COLTURATO, ALFREDO RIBEIRO-SILVA, ANDRESSA DUARTE, SUZIENE CAROLINE DA SILVA, LUCIANA YAMAMOTO ALMEIDA, RODRIGO NEVES SILVA and, JORGE ESQUICHE LEON Objective: Squamous cell carcinoma (SCC) of the head and neck region with neuroendocrine differentiation are rare neoplasms. These tumors can be poorly differentiated and usually have tendency for aggressive clinical behavior and propensity for both regional and distant metastasis. Their diagnosis is based on morphology and confirmation of origin by immunohistochemistry. The purpose of this study was to investigate the neuroendocrine differentiation profile in oral SSC (OSCC) and oropharyngeal SCC (OPSCC). Study Design: One hundred thirteen cases (male, n = 95; female, n = 18; mean age, 60.4 years) diagnosed with OSCC (n = 85) and OPSCC (n = 28) were selected. Immunohistochemistry was performed using the tissue microarray technique to evaluate the immunomarkers chromogranin-A, synaptophysin, CD56, CD57, CD99, Ki-67, and cyclin D1. Results: Our results showed positivity for chromogranin-A (8.3% OSCC; 0% OPSCC), synaptophysin (9.5% OSCC; 0% OPSCC), CD99 (28.8% OSCC; 3.8% OPSCC) and CD56 (1.2% OSCC; 11.1% OPSCC). CD57 was negative. The Ki-67 was 43.6% and 42.0% and cyclin D1 was 33.7% and 26.6% in OSCC and OPSCC, respectively. Conclusions: Our results show similar proliferation index in both OSCC and OPSCC, and that neuroendocrine differentiation can be detected in some cases of OSCC and OPSCC and should be reclassified as such. FAPESP grant: 2016/11419-0.
FOXP3+ REGULATORY T CELLS IN ORAL TONGUE SQUAMOUS CELL CARCINOMA IN YOUNG AND OLDER PATIENTS: IMMUNOHISTOCHEMICAL STUDY. EMANUENE GALDINO PIRES, LUANA SAMARA BALDUINO DE SENA, MARINA GONCALVES ¸ DO AMARAL, DENIZE NUBIA SOUZA, ^ e MANUEL ANTONIO GORDON-NUNEZ, POLLIANNA MUNIZ ALVES and, CASSIANO FRANCISCO WEEGE NONAKA Objective: To determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of oral tongue squamous cell carcinoma (OTSCC) in young (45 years) and older (60 years) patients and to correlate the findings with clinicopathologic parameters (sex, tumor size/extent, regional lymph node metastasis, clinical stage, and histopathologic grade of malignancy).
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Results: The IC50 of HBEE for MCF-7 tumor cells (36.28 mg/mL) was significantly higher than for MCF-10 A nontumor cells (145.13 mg/mL). In vivo assay demonstrated that HBEE at 30 mg/Kg significantly reduced tumor weight (P < .05) and Ki-67 expression (P < .01), and increased apoptosis index (P < .001) and leukocyte count in peripheral blood (P < .05) in comparison with CTR. Conclusions: These data suggest that the HBEE exerted selective cytotoxic for tumor cells and antitumor activity comparable to 5-FU.
EXPRESSION OF TETRASPANINS CD9 AND CD63 IN HEAD AND NECK SQUAMOUS CELL CARCINOMA: AN IMMUNOHISTOCHEMICAL STUDY. RODRIGO NEVES-SILVA, ALFREDO RIBEIRO SILVA, ANDRESSA DUARTE, SUZIENE CAROLINE SILVA, LUCIANA YAMAMOTO ALMEIDA, CARLA BENTO NELEM COLTURATO and, JORGE ESQUICHE LEON Objective: Tetraspanins are proteins involved in several cellular processes, including association with tumor progression. In this study, the immunoexpression of tetraspanins in head and neck squamous cell carcinoma (HNSCC) were assessed, aiming to evaluate the participation of these molecules in carcinogenesis. Study Design: An immunohistochemical study was carried out using the tissue microarray technique to evaluate the tetraspanins CD9 and CD63. One hundred and twenty-one HNSCCs were analyzed (male, n = 97; female, n = 24; mean age, 60.4 years). The anatomic sites were 84 oral cavity, 23 oropharynx, 3 skin, 7 paranasal sinuses, 2 larynx, 1 nasopharynx, and 1 pharynx. Results: Only 103 HNSCCs were available for analysis. For CD9, 69 (67%) HNSCCs were positive (46 oral cavity, 17 oropharynx, 5 paranasal sinuses, and 1 skin). For CD63, 11 (10.7%) HNSCCs were positive (7 oral cavity, 2 oropharynx, 1 paranasal sinuses, and 1 skin). Conclusions: Our results suggest participation of tetraspanins CD9 and CD63 in the HNSCC pathogenesis. Further studies are necessary to determinate their prognostic effect with therapeutic implications. FAPESP grant 2016/11419-0.
DIAGNOSTIC VALUE OF MICRO RNA BIOMARKERS AS AN EARLY DIAGNOSTIC METHOD OF ORAL SQUAMOUS CELL CARCINOMA. CRISTIAN MAURICIO ROJAS and, MATHIAS MAURICIO PARRA APABLAZA Background: To date, biopsy is the gold standard for diagnosis of oral squamous cell carcinoma (OSCC). Due to its limitations and late diagnosis of OSCC, it’s necessary to develop new techniques that allow an early diagnosis of oral cancer. Recent studies indicate that micro RNAs biomarkers (miRNAs) present in body fluids such as blood, plasma, serum, and saliva offer an effective method for the early diagnosis of OSCC. Objective: To describe the diagnostic value of miRNAs biomarkers in body fluids as a method of early diagnosis of OSCC. Method: An electronic search was carried out in the following databases: Pubmed, Trip Database, Epistemonikos, Cochrane Library, Scopus, Ebsco, and Bireme. From the selected studies, a retrograde search was performed. Results: A total of 24 articles, published between 2008 and 2017, met the selection criteria and were included in this
OOOO January 2020 review. The studies support the use of miRNAs biomarkers as a potential tool to obtain an early diagnosis of OSCC. Conclusions: According to the studies analyzed in this review, miRNAs biomarkers in body fluids have an undoubted potential, although their diagnostic value should be considered moderate until new studies demonstrate and validate their use as an early diagnosis method of OSCC.
NEUROENDOCRINE DIFFERENTIATION AND CELL PROLIFERATION IN ORAL AND OROPHARYNGEAL SQUAMOUS CELL CARCINOMA. CARLA BENTO NELEM COLTURATO, ALFREDO RIBEIRO-SILVA, ANDRESSA DUARTE, SUZIENE CAROLINE DA SILVA, LUCIANA YAMAMOTO ALMEIDA, RODRIGO NEVES SILVA and, JORGE ESQUICHE LEON Objective: Squamous cell carcinoma (SCC) of the head and neck region with neuroendocrine differentiation are rare neoplasms. These tumors can be poorly differentiated and usually have tendency for aggressive clinical behavior and propensity for both regional and distant metastasis. Their diagnosis is based on morphology and confirmation of origin by immunohistochemistry. The purpose of this study was to investigate the neuroendocrine differentiation profile in oral SSC (OSCC) and oropharyngeal SCC (OPSCC). Study Design: One hundred thirteen cases (male, n = 95; female, n = 18; mean age, 60.4 years) diagnosed with OSCC (n = 85) and OPSCC (n = 28) were selected. Immunohistochemistry was performed using the tissue microarray technique to evaluate the immunomarkers chromogranin-A, synaptophysin, CD56, CD57, CD99, Ki-67, and cyclin D1. Results: Our results showed positivity for chromogranin-A (8.3% OSCC; 0% OPSCC), synaptophysin (9.5% OSCC; 0% OPSCC), CD99 (28.8% OSCC; 3.8% OPSCC) and CD56 (1.2% OSCC; 11.1% OPSCC). CD57 was negative. The Ki-67 was 43.6% and 42.0% and cyclin D1 was 33.7% and 26.6% in OSCC and OPSCC, respectively. Conclusions: Our results show similar proliferation index in both OSCC and OPSCC, and that neuroendocrine differentiation can be detected in some cases of OSCC and OPSCC and should be reclassified as such. FAPESP grant: 2016/11419-0.
FOXP3+ REGULATORY T CELLS IN ORAL TONGUE SQUAMOUS CELL CARCINOMA IN YOUNG AND OLDER PATIENTS: IMMUNOHISTOCHEMICAL STUDY. EMANUENE GALDINO PIRES, LUANA SAMARA BALDUINO DE SENA, MARINA GONCALVES ¸ DO AMARAL, DENIZE NUBIA SOUZA, ^ e MANUEL ANTONIO GORDON-NUNEZ, POLLIANNA MUNIZ ALVES and, CASSIANO FRANCISCO WEEGE NONAKA Objective: To determine the number of FoxP3+ regulatory T (Treg) cells in the microenvironment of oral tongue squamous cell carcinoma (OTSCC) in young (45 years) and older (60 years) patients and to correlate the findings with clinicopathologic parameters (sex, tumor size/extent, regional lymph node metastasis, clinical stage, and histopathologic grade of malignancy).