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F.47. Evaluation of CD4+ T Cell Count, Interleukin 6 and Interferon Gamma in HIV Infected Symptomatic and Asymptomatic Individual
S58 leishmaniasis (kala-azar), represents the most severe clinical manifestation of Leishmania infection. Sevaral factors can affect the outcome of an infection, which of them are genetic factors. In this study we tried to find any relationship between kala-azar and IL-6 gene polymorphism. Methods: One hundred and ten patients with kala-azar, 59 healthy patients' siblings and 87 healthy individuals with positive Leihmanin skin test but without any history leishmaniasis, joined this study. DNAs extracted from samples were genotyped for IL-6 (-174 G/C) polymorphism using allele specific polymerase chain reaction (AS-PCR) method. Results: Our results showed that IL-6 (-174) gene polymorphism was distributed similarly in the patient and the two control groups. Discussion: Although, we found no association between IL-6 (-174) gene polymorphism and susceptibility to kala-azar, we cannot exclude that other polymorphisms within this gene or its receptor may donate susceptibility to the disease. Moreover these findings need to be confirmed in other populations. doi:10.1016/j.clim.2008.03.157
F.46. Lack of Association Between Toll-like Receptor 4 (TLR4) Asp299Gly Polymorphisms and Susceptibility to Visceral Leishmaniasis (Kala-azar) Simin Kiany,1 Manoochehr Rasouli,2 Maryam Behbin.2 1 Shiraz University of Medical Sciences- Clinical Microbiology Research Center, San Jose, CA; 2Shiraz University of Medical Sciences, Clinical Microbiology Research Center, Shiraz, Iran Introduction: Toll-like receptor4 (TLR4) is a pattern recognition receptor that contributes to the control of Leishmania parasite growth in parasitized macrophages and helps them to kill the parasite more efficient. Indeed, signaling through TLR4 has been associated with the induction of Th1 responses and a lack of signaling has been associated with the induction of Th2 responses. Since the expression of TLR4 can be affected by a functional polymorphism within TLR4 gene (TLR4 Asp299Gly), we tried to find any probable relation between this polymorphism and susceptibility to visceral leishmaniasis (kala-azar). Methods: One hundred and nineteen pediatric patients involved with visceral leishmaniasis and 57 healthy persons from the same area as patients, were included in this study. TLR4 Asp299Gly genotyping was carried out using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Results: Our results didn't show any statistical significant differences between alleles and genotypes frequencies among studied groups (p = 0.35). Discussion: Our analysis did not reveal a significant difference between the frequencies of TLR4 Asp299Gly genotypes and alleles. It might be the result of our limited number of controls. We suggest this study be continued on larger population of the controls. Also, analysis of polymorphism in other positions of TLR4 gene is recommended. doi:10.1016/j.clim.2008.03.158
Abstracts
F.47. Evaluation of CD4+ T Cell Count, Interleukin 6 and Interferon Gamma in HIV Infected Symptomatic and Asymptomatic Individual Martin Ifeanyichukwu. Nnamdi Azikiwe University, Nnewi Campus, Nigeria Aim: The study was designed to assess the effect of HIV infection on CD4 count,interleukin-6,gamma interferon in HIV infected subjects, who were not administered antiretroviral therapy (ART). Method: 45 HIV positive subjects (male = 27, female = 18), aged between 15-65 years were recruited for the study. 21 of this (male = 10, female = 11) were symptomatic subjects, while the remaining 24 subjects (male = 17, female = 7), were asymptomatic. Similarly, 22 HIV seronegative subjects served as control. Blood samples were colleted from the participants for the determination of HIV status by immunochromatography method, CD4+T cell count by Flow Cytometry,gamma interferon and interleukin-6 by ELISA method. Result: The result of the present study showed diminished CD4+T cells count in symptomatic HIV infected subjects compared with corresponding values in the asymptomatic HIV subjects (p b 0.05). Gamma interferon in symptomatic HIV infected subjects showed diminished concentration compared respectively, with the asymptomatic HIV infected subjects and control subjects (P b 0.05 in each case). However, there was no significant difference in the parameter between the asymptomatic HIV seropositive and the control HIV seronegative subjects (p N 0.05). Meanwhile, the serum interleukin-6 concentration were similar amongst the three groups (p N 0.05). Discussion and conclussion: The observed decline in the CD4 and gamma interferon in the symptomatic stage of HIV infection suggests immune distortion of effectors mechanism responsible for cellular immune responses. The serum interleukin-6 concentration which was observed to be similar among the three groups suggests that antibody mediated responses may not be impaired at this stage of HIV infection. doi:10.1016/j.clim.2008.03.159
F.48. Interleukin-18 Gene Polymorphisms are Associated with Brucellosis in Iranian Patients Manoochehr Rasouli, Maryam Behbin, Simin Kiany. Shiraz University of Medical Sciences- Clinical Microbiology Research Center, Shiraz, Iran Background: Interleukin-18 (IL-18) is a pleiotropic cytokine that is involved in regulation of both the innate and acquired immune response. The most prominent biologic property of IL-18 is its ability to induce the production of IFNgamma in presence of IL-12. Moreover, it stimulates the expression of TNF-α and IL-1, enhances the differentiation of T cells to the Th1 and impairs the synthesis of the antiinflammatory cytokine IL-10. Then it seems that IL-18 has a crucial role in immunity against Brucella infection. Since the expression of IL-18 can be affected by polymorphisms in its gene, we decided to investigate any probable relation between six different IL-18 gene polymorphisms and brucellosis. Methods: A total of 188 patients with brucellosis and 77
F.46. Lack of Association Between Toll-like Receptor 4 (TLR4) Asp299Gly Polymorphisms and Susceptibility to Visceral Leishmaniasis (Kala-azar) Simin Kiany,1 Manoochehr Rasouli,2 Maryam Behbin.2 1 Shiraz University of Medical Sciences- Clinical Microbiology Research Center, San Jose, CA; 2Shiraz University of Medical Sciences, Clinical Microbiology Research Center, Shiraz, Iran Introduction: Toll-like receptor4 (TLR4) is a pattern recognition receptor that contributes to the control of Leishmania parasite growth in parasitized macrophages and helps them to kill the parasite more efficient. Indeed, signaling through TLR4 has been associated with the induction of Th1 responses and a lack of signaling has been associated with the induction of Th2 responses. Since the expression of TLR4 can be affected by a functional polymorphism within TLR4 gene (TLR4 Asp299Gly), we tried to find any probable relation between this polymorphism and susceptibility to visceral leishmaniasis (kala-azar). Methods: One hundred and nineteen pediatric patients involved with visceral leishmaniasis and 57 healthy persons from the same area as patients, were included in this study. TLR4 Asp299Gly genotyping was carried out using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). Results: Our results didn't show any statistical significant differences between alleles and genotypes frequencies among studied groups (p = 0.35). Discussion: Our analysis did not reveal a significant difference between the frequencies of TLR4 Asp299Gly genotypes and alleles. It might be the result of our limited number of controls. We suggest this study be continued on larger population of the controls. Also, analysis of polymorphism in other positions of TLR4 gene is recommended. doi:10.1016/j.clim.2008.03.158
Abstracts
F.47. Evaluation of CD4+ T Cell Count, Interleukin 6 and Interferon Gamma in HIV Infected Symptomatic and Asymptomatic Individual Martin Ifeanyichukwu. Nnamdi Azikiwe University, Nnewi Campus, Nigeria Aim: The study was designed to assess the effect of HIV infection on CD4 count,interleukin-6,gamma interferon in HIV infected subjects, who were not administered antiretroviral therapy (ART). Method: 45 HIV positive subjects (male = 27, female = 18), aged between 15-65 years were recruited for the study. 21 of this (male = 10, female = 11) were symptomatic subjects, while the remaining 24 subjects (male = 17, female = 7), were asymptomatic. Similarly, 22 HIV seronegative subjects served as control. Blood samples were colleted from the participants for the determination of HIV status by immunochromatography method, CD4+T cell count by Flow Cytometry,gamma interferon and interleukin-6 by ELISA method. Result: The result of the present study showed diminished CD4+T cells count in symptomatic HIV infected subjects compared with corresponding values in the asymptomatic HIV subjects (p b 0.05). Gamma interferon in symptomatic HIV infected subjects showed diminished concentration compared respectively, with the asymptomatic HIV infected subjects and control subjects (P b 0.05 in each case). However, there was no significant difference in the parameter between the asymptomatic HIV seropositive and the control HIV seronegative subjects (p N 0.05). Meanwhile, the serum interleukin-6 concentration were similar amongst the three groups (p N 0.05). Discussion and conclussion: The observed decline in the CD4 and gamma interferon in the symptomatic stage of HIV infection suggests immune distortion of effectors mechanism responsible for cellular immune responses. The serum interleukin-6 concentration which was observed to be similar among the three groups suggests that antibody mediated responses may not be impaired at this stage of HIV infection. doi:10.1016/j.clim.2008.03.159
F.48. Interleukin-18 Gene Polymorphisms are Associated with Brucellosis in Iranian Patients Manoochehr Rasouli, Maryam Behbin, Simin Kiany. Shiraz University of Medical Sciences- Clinical Microbiology Research Center, Shiraz, Iran Background: Interleukin-18 (IL-18) is a pleiotropic cytokine that is involved in regulation of both the innate and acquired immune response. The most prominent biologic property of IL-18 is its ability to induce the production of IFNgamma in presence of IL-12. Moreover, it stimulates the expression of TNF-α and IL-1, enhances the differentiation of T cells to the Th1 and impairs the synthesis of the antiinflammatory cytokine IL-10. Then it seems that IL-18 has a crucial role in immunity against Brucella infection. Since the expression of IL-18 can be affected by polymorphisms in its gene, we decided to investigate any probable relation between six different IL-18 gene polymorphisms and brucellosis. Methods: A total of 188 patients with brucellosis and 77