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Flow cytometry (2nd edn)
1626
Book Reviews
from the primary structure of proteins; solid phase peptide synthesis; immunization with peptide carrier complexes; preparative immu~toaffinitytechniques; immunoassays of proteins and anti-peptide antibodies; epitope mapping using synthetic peptides; epitope mapping using libraries of random peptides displayed on phage; DNA encoded amino acids.
Progress in Iron Research-Edited by C. Hershko, A. M. Konijn and P. Aisen. 402 pp. 1994. Plenum Press, New York. $110.
The continued interest in the role of iron in the body is reflected in this account of the 4th International Conference on Hernochromatosis and iron and iron proteins. The main topics are: biophysical aspects of iron compounds; transferrins and transferrin receptor physiology; iron absorption and uptake; control of iron protein gene expression; ferritin metabolism; erthyroid iron regulation; pathophysiology of iron disorders; epidemiology and genetics of hemochromatosis; development and applications of iron chelators.
Flow Cytometry (2nd edn)--Edited by Z. Darzynkiewicz, J. P. Robinson and H. A. Crissman. Part A. Methods in Cell Biology, 'Col. 41. 586 pp. 1994. Academic Press, San Diego. HB $110; PB $59.95. Part B. Methods in Cell Biology, Vol. 42. 692 pp. 1994. Academic Press, San Diego. HB $110; PB $59.95.
Flow cytometry of isolated cells enables the mechanical automatic sorting of cells at the rate of several thousand a second into different classes and subclasses. Rare cells can be detected. It can also can be used to define the epitopes on cells using forward light scatter, and side scatter of several antibodies, labelled with different colored fluorochromes. The first edition of this book was published in 1990, and since then, the technique has been widely used and applied to in situ hybridization; mRNA species detection; chromosome sorting; detection of intracellular viruses and virus proteins; analysis of cell proliferation; multivariate analysis of DNA vs protein or DNA vs RNA; BrdUrd and IdUrd incorporation; GI and (32 cyclin proteins; somatic mutations; micronuclei; DNA repair; lymphocyte phenotyping; reticulocyte and platelet analysis; hematopoietic stem cell sorting; DNA ploidy; cells in tumors; HIV infection; drug resistant cells. The techniques open up a whole new world of understanding what the different cells and cell components are doing in a multicellular organ.
Molecular Biology of Mitoehondrial Transport Systems Edited by M. Forte and M. Colombini. 408 pp. 1994. Springer Verlag, Berlin. DM 248.
In brown fat the adipocytes have an uncoupling protein (UCP) that uncouples mitochondrial ATP synthesis from the respiratory chain and is responsible for heat production by brown fat. UCP is a proton (H ÷) membranous transporter and is also able to transport C1- ions. Cl- transport is inhibited by purine nucleotides while free fatty acids facilitate H ÷ translocation. UCP belongs to a family which includes the adenine nucleotide translocator, the phosphate carrier and the oxoglutarate carrier. They have a polypeptide chain of about 100 amino acids in three repeated sequences, probably derived by triplication from an ancestral gene. This volume deals with: UCP and ATPase; carriers and transporters; mitochondrial ion channels; voltage sensitive VDAC channel; VDAC, peripheral kinases and energy utilization; mitochondrial channels in humans in relation to disease. A Practical Guide to Membrane Protein Purification; Separation, Detection and Characterization of Biological Maeromolecules--Edited by G. von Jagow and H. Schagger. 166 pp. 1994. Academic Press, San Diego. PB $49.95.
Membrane proteins are more difficult to purify than are water soluble proteins. This book provides practical details how to separate membrane proteins at low cost. The chapters deal with: purification strategies; chromatographic techniques; denaturing electrophoresis; native gel electrophoresis; isolation of complex III, subcomplexes and protein subunits from bovine heart; purification of F~Fo ATPase and Complex I from heart mitochondria; isolation of ubiquinol: cytochrome c oxidoreductase; isolation of Wolinella hydrogenase; isolation in crystalline form by preparation isoelectric focusing of photosystem I. Full practical details and the theories behind them are provided. Physicochemical Methods in the Study of BiomembranesEdited by H. J. Hildren and G. B. Ralston. 509 pp. 1994. Plenum Press, New York. $120. [SubcellularBiochemistry, Vol. 23]
Although it is necessary to test drugs on whole animals, the study of drug action on cell membranes from different organs can be of great value. This volume provides details of some of the methods used in studying the properties of biomembranes. They include intracelhilar lipid distribution, transport and sorting; cytoskeleton proteins; monomolecular layers; differential scanning and dynamic calorimetry; ektacytometry of red cells; spin label ESR of lipid/protein dynamics; NMR measurement of membrane transport; Fourier Transform infra red spectrosopy; X-ray diffraction of lipids. The main emphasis is on the methodology and the value and importance of the results obtained by the different techniques.
Book Reviews
from the primary structure of proteins; solid phase peptide synthesis; immunization with peptide carrier complexes; preparative immu~toaffinitytechniques; immunoassays of proteins and anti-peptide antibodies; epitope mapping using synthetic peptides; epitope mapping using libraries of random peptides displayed on phage; DNA encoded amino acids.
Progress in Iron Research-Edited by C. Hershko, A. M. Konijn and P. Aisen. 402 pp. 1994. Plenum Press, New York. $110.
The continued interest in the role of iron in the body is reflected in this account of the 4th International Conference on Hernochromatosis and iron and iron proteins. The main topics are: biophysical aspects of iron compounds; transferrins and transferrin receptor physiology; iron absorption and uptake; control of iron protein gene expression; ferritin metabolism; erthyroid iron regulation; pathophysiology of iron disorders; epidemiology and genetics of hemochromatosis; development and applications of iron chelators.
Flow Cytometry (2nd edn)--Edited by Z. Darzynkiewicz, J. P. Robinson and H. A. Crissman. Part A. Methods in Cell Biology, 'Col. 41. 586 pp. 1994. Academic Press, San Diego. HB $110; PB $59.95. Part B. Methods in Cell Biology, Vol. 42. 692 pp. 1994. Academic Press, San Diego. HB $110; PB $59.95.
Flow cytometry of isolated cells enables the mechanical automatic sorting of cells at the rate of several thousand a second into different classes and subclasses. Rare cells can be detected. It can also can be used to define the epitopes on cells using forward light scatter, and side scatter of several antibodies, labelled with different colored fluorochromes. The first edition of this book was published in 1990, and since then, the technique has been widely used and applied to in situ hybridization; mRNA species detection; chromosome sorting; detection of intracellular viruses and virus proteins; analysis of cell proliferation; multivariate analysis of DNA vs protein or DNA vs RNA; BrdUrd and IdUrd incorporation; GI and (32 cyclin proteins; somatic mutations; micronuclei; DNA repair; lymphocyte phenotyping; reticulocyte and platelet analysis; hematopoietic stem cell sorting; DNA ploidy; cells in tumors; HIV infection; drug resistant cells. The techniques open up a whole new world of understanding what the different cells and cell components are doing in a multicellular organ.
Molecular Biology of Mitoehondrial Transport Systems Edited by M. Forte and M. Colombini. 408 pp. 1994. Springer Verlag, Berlin. DM 248.
In brown fat the adipocytes have an uncoupling protein (UCP) that uncouples mitochondrial ATP synthesis from the respiratory chain and is responsible for heat production by brown fat. UCP is a proton (H ÷) membranous transporter and is also able to transport C1- ions. Cl- transport is inhibited by purine nucleotides while free fatty acids facilitate H ÷ translocation. UCP belongs to a family which includes the adenine nucleotide translocator, the phosphate carrier and the oxoglutarate carrier. They have a polypeptide chain of about 100 amino acids in three repeated sequences, probably derived by triplication from an ancestral gene. This volume deals with: UCP and ATPase; carriers and transporters; mitochondrial ion channels; voltage sensitive VDAC channel; VDAC, peripheral kinases and energy utilization; mitochondrial channels in humans in relation to disease. A Practical Guide to Membrane Protein Purification; Separation, Detection and Characterization of Biological Maeromolecules--Edited by G. von Jagow and H. Schagger. 166 pp. 1994. Academic Press, San Diego. PB $49.95.
Membrane proteins are more difficult to purify than are water soluble proteins. This book provides practical details how to separate membrane proteins at low cost. The chapters deal with: purification strategies; chromatographic techniques; denaturing electrophoresis; native gel electrophoresis; isolation of complex III, subcomplexes and protein subunits from bovine heart; purification of F~Fo ATPase and Complex I from heart mitochondria; isolation of ubiquinol: cytochrome c oxidoreductase; isolation of Wolinella hydrogenase; isolation in crystalline form by preparation isoelectric focusing of photosystem I. Full practical details and the theories behind them are provided. Physicochemical Methods in the Study of BiomembranesEdited by H. J. Hildren and G. B. Ralston. 509 pp. 1994. Plenum Press, New York. $120. [SubcellularBiochemistry, Vol. 23]
Although it is necessary to test drugs on whole animals, the study of drug action on cell membranes from different organs can be of great value. This volume provides details of some of the methods used in studying the properties of biomembranes. They include intracelhilar lipid distribution, transport and sorting; cytoskeleton proteins; monomolecular layers; differential scanning and dynamic calorimetry; ektacytometry of red cells; spin label ESR of lipid/protein dynamics; NMR measurement of membrane transport; Fourier Transform infra red spectrosopy; X-ray diffraction of lipids. The main emphasis is on the methodology and the value and importance of the results obtained by the different techniques.