Gas-liquid chromatography of mimosine

Gas-liquid

chromatography

of mimosine*

o~‘c’Urrin~ (I), ill So lillc~~V11 :LS Ic!ll~‘C!ll~~l, is it TlilttllYLIl~ /.c*i/l*r/i’l/rt fi’//roc’t’~l/i//t/ trcJl)iv;tI lqymiilous l~liLlltS, ~‘.g.

Miliiosinc:

fr-JLlllCl irl HO 0

-t:

I’IW! ;tnlinf

( 1.iLI11,)

I ilC*itt

dlt

I\‘it,

yz

-

N -CH2CHCOOH

-

(I) fCJI’IllLlli1 iS ~-ll~‘tl~OS?‘-~-‘JS( Jilllcl :Iii~ll~~Stl /)rldicrr1,. ‘I’IIC stl+LlCtLlriLl 1.. ,&rr/ctr J-h!lltll., IlCCiLI_lS;C J (~lI-J)-l~~~1~itli1~l!;LliL1li1~C!,;\s iL t>wJSinc iL1liLI(J~LIl!, tlliS ariLincJ iLCi\SIJ \j’;\\‘.\I..\S’ ~Vl!l’C!aIS0 c’o1ll-C!r’Illxt of its toxic c!l’fci*t 011 ~l0ll-rLlllLillillltS’~~, fOlJd in SOLltlll!ilSt iLl>OLlt tilt! IlSl? Of Sl!l!tlS illltl Sll~~~Ull~llt tip Of tllC!%! l>lilllt. C; for llLlllliLll .A\Siil. 111 tlic

~*oul’sic of StllcliCs

l’Il~0Illi~t~~~~iL~~lI~’

liriio

\vllic:ll

is

clistinc:t

S..tt’illuor;~~~ct~‘l tllis iwicl

rcl)ort iti

it

is to

Liniclul!

frown

(N-*1*1;:\) Sllo\V

011 tlli*

it\‘;~iliLl)ilit~*

it \\‘;lS fOLlllC1 tllilt

(C;l.C),

tllilt

Scl~ilriltif

~~o~iiiiio~il~~

u-hut)*1 mimosinc Ill

Of till*

cstcr

nliiiic li~ioux

l)y

(;I.(.*

(rf ~m~t~~iii i1llliIlO Isinc

iLIllillCJ on

Vi111 IX? i~llilI~‘Zl!Cl

SiIIll~!

itlJlX!ilIX

iU:iC.lS

ill1 EC;:\ 11>’ (;T.(::

C~011111111 ilIOI1~ lvitli

avirls

tllc

ilt

I.,* gas-licluicl

sliortwt

wtcwtion

\Vlll!lliLSSlL~‘l!CI lY~l1111111. ‘I’llC

ilS

ptirpow

tllcit Of

non-protein amino lwotcin illllill(J ilciCl.

i1S ;l

tllc

g:1’(JLlIlcl dr\* /.. g:‘lrfucct Ivitll c*r)ld 0.1 .\’ I-I(‘l. t0 j lllg clq* lllil.tr!riill) fVilS introclucixl tli t’c*c:tl~ into il (:ollc-SIlillwCl mic’rr~-k’iil.1 (O,.j 1111\‘olLllllc~) n*itlloLlt C+ll!illl-Lll). ‘I’llc clcri\*ati%iLti(Jll lm Jccdurc \V;LS (1) clirwt cStc.Titic’iLticJIl :Lllcl (3) iK’>‘liLtic 111 ;~cwwcling tc I I. U-glrws wlutllll 011 SO-100 IllcSll il.\\‘. 14.1’ Cl1ronioSorl~ G (lw~~l1catocl IJiLC’kCCI $Vitll O.:jZj \V/tV ‘:& IX.4 After

Alinlc 6inl:

\g’ilS

liltratir~n,

ill1

‘I’;\ ISI .I< I

l!Stril~‘tl*Cl

i\liflllOt

1’1’0111 liiic!l>*

Of 0. I 1111(l~~lLli\‘illl!1lt

ILE

ALb

SER

WE

GLU

LYS

TYA

AdA -'.~"---i_da-'--_-~-i~~--~-~~~'-.12~-~c -.

-.‘...““i~----.__..‘~i;~_.~‘-‘__~~______.__i~.~-.__-n~--’ U,H

~liilvrsinc~ Wtclr

(or

ri lnili

qo

Illil~k~Cll~~ I’c!il~.*ti0ll

0.5

lliiri

iIlc*rl!ilSc!Cl

17Ci'

~x.~il; (Fig. ‘I’llc is

;tt

Ic:,

:LliiiticbiIIlC1 pllcriolic:

‘I’llc

isotl~crtli;tll~*)

min.

Stitl>ilitja

n~inu~siiic!

tllcS;c

;llilllill~~

ilC*i(l 011

~~rL!S;tllllillJl~~ (IlloW

\t’;L.C; fOrllllg~l

c*o1lclition~,

is of iritcrost to

illllill0 of

illclic~iltillg ~~rOClLl~‘t

lillcll*~

;dclitinn

itlld

tll;lt

I’f C’SC’CSS

dr!ri\*ilti\Y

~l.liL11iclf1

ll~ll1-~~rlJtl~ill tllilll

iI lllOllO;l~‘~‘l

iLCCt~'l~ltl.!Cl in

stllfll~Ylr~dl,

lirst

rctc~ntioll

\‘clliltilit>‘.

rlftiT

I). diilCj*l

is tlli!

SllclrtCr

Illi~mb\*iid,

IJC.@ilkilt

ring

fill’

so

f~~LlIl
tllilll illSO

S-t~iil~li~roil~.~i~t~~l I:(;.-\.

lt

;I (Ii-ill*\*1 100 4x*iilg h

g)

Iwc*:l~l~~tllit

inliuo,

~NWSt!llt

;L ~l!litti\‘l:l\’

;~-l1~*cll*os~~ grrjllll

I IO’

clori\*iLti\*c

\V;lS

it \\‘l~111~1 sillpwirll~~ow

t~illLlol'~~i~cct~~l;ltcc.l ;Lnlillo,

/r-l>llt>*l

c@lUteS ilt

frbr tvitll

ill

tllc

stiirtllcr

011

tllc! 1Wolillc

c-111

lq’riclonc.+f

Iljxlrc~s~‘,

I)llcncblir*,

‘I’lw CliilCl\‘l t~*rosi~~~ c!stc:r (TIT:\ on iii~icl~ixolc groups rq~ortcd. group), Iio\vu~‘c~r, clutus ;it 203-“,‘25 Ill111 l.llldcl~ tllc Siltlll.! c:oll~litiotlS. of miiiic~dilir! to Ilot, ~*(~ll~l!Iltr’iltl!C~ ilC:i(l is ;IIso of intcrc!st to t.llis

I-l.3

SlJ’I’liS

SC.!~\‘l!Cl

in

CliC:iLtl!CI :\

illl?’

(II’

I)>* nits

0111

lgcll

stutiics.

f~llillltitilti\‘C:

(;I.(.:

ilCltli!l~

il I
~Llll~~llllt

mtion.

‘I’llis,

tUl~ll,

illtCl’tliLI nxticm

tluring

iI1

Sti~llClil~~l I’ol’

‘1.‘11(!

ilcl~)l’Ul

ill1 I>l:

Or

illt~:~l1i11 ~.:illiI~r’~ltl!Cl

ill~~~~~l~Clil1~ Of

is Cllll! t0

tilt:

Cilc’t

tllilt

110

rcsiclric:

illilIlill~!

\\‘iLS

in-

I :\).

t0

S;cll\‘Cllt,

Inim~sinc

(.‘;I11 I>l!

StilIlCliLr’Cl :lgiLiIlSt

(~1~lll~l
(1,s.) il

l+l f/l.“. of

to

riiisturc :\I1

lillill

l*c!il(.lilJ* ilClliC\*CCl tllc

(!.StriLct

prior

Of nlimcbsinc illtl~r’llill

Clcri\‘itti-

Stil.llCliLI.C1

il!l(l

pornlits

1’01’-

StilllCliLt’Cl

Clc!ri\‘iLti\‘C’

II)’ diroc:tl~* tfl

mistur’c!,

illltl

If tSSt.5

Ill;llli~~lllilti~~ll,

iLl_ltll~Il%

l~ic~~l~crll.istr~~~, ilIlC1

I’LlI

(I:ig, ilIlill\*S;iS

of

(:il.Il

\‘oIilti%iltic~ll

Sillll]~lO

‘I’llis

clctcctc~l*

\visll

Clnit+crsit>*

cliscwssicms.

tf) of

tlliLllli I-l~L\Vitii,

I.)r. fr)I’

J~~lIIS IliS

\I’.

I-II’l.IS,

:LSSiStiLll~~~!

I)~!~~ilt’tllll!Ilt

in i~I’o~‘idilIg

Of

ri~iliiosini!

;\~l’i(‘lllttll’iLI StilllClilI’Cl