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Granular Cell Tumor: Histopathological and Immunohistochemical Study of 25 New Cases
OOOO Volume 117, Number 2 SANDRA TORRES. UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. Oral manifestations are common in patients with chronic graft-versus-host disease (cGVHD). However, few studies present systematic results on the prevalence of its different clinical presentations. This study evaluates the frequency of oral cGVHD features. Materials and Methods: Patients with symptomatic oral cGVHD were studied. Demographic and clinical data were taken from medical records. Oral features were assessed through National Institutes of Health (NIH) criteria. A descriptive analysis was performed. Results: Twenty-two patients were included, 43.8% of whom were male. Patients presented diagnostic (87.5%), distinctive (100%), and common (100%) features of oral cGVHD. The gingiva was the most commonly affected anatomical site. Ulceration was the most frequent type of oral lesion, which is related to the inclusion of patients with symptomatic cGVHD. Salivary gland dysfunction was observed in 87.5% of cases. Conclusions: Oral involvement was frequent in the population studied. The calibration of investigators is important for an adequate diagnosis of oral cGVHD.
PE-397 - GLUT-1 AS A TOOL FOR THE CORRECT DIAGNOSIS OF HEMANGIOMAS: AN UPDATE OF ALL CASES DIAGNOSED AT AN ORAL PATHOLOGY SERVICE. TIAGO JOAO DA SILVA FILHO, DENISE HÉLEN IMACULADA PEREIRA DE OLIVEIRA, LUCIANA ELOÍSA DA SILVA CASTRO, ANDRÉIA FERREIRA DO CARMO, RODRIGO GADELHA VASCONCELOS, PATRÍCIA TEIXEIRA DE OLIVEIRA, LÉLIA MARIA GUEDES QUEIROZ. UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE. This study analyzed the immunohistochemical expression of human glucose transporter proteins (GLUT-1) in all cases diagnosed as hemangiomas from a service of Oral Pathology and reclassified such lesions according their immunoexpression. Study Design: A total of 79 cases histologically diagnosed as hemangiomas were retrieved from a total of 12,100 lesions diagnosed at the Division of Oral Pathology, Federal University of Rio Grande do Norte, between 1970 and 2013. For all those lesions, we assessed the immunohistochemical expression of GLUT-1. Some cases were reclassified as pyogenic granulomas (PG) and vascular malformations (VM). Results: This research showed that only 26 cases initially classified as hemangiomas presented immunopositivity for GLUT-1 and fit the initial diagnosis. The remaining 53 cases were reclassified as PG (20 cases) and VM (33 cases). Conclusion: This study showed that the histological diagnosis alone is not always enough for the correct diagnosis of infantile hemangiomas.
PE-398 - GRANULAR CELL TUMOR: HISTOPATHOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY OF 25 NEW CASES. MARISOL MARTÍNEZ MARTÍNEZ, MARISOL MARTÍNEZ MARTÍNEZ, FELIPE PAIVA FONSECA, THAMYRES CAMPOS FONSECA, MICHELLE AGOSTINI, OSLEI PAES DE ALMEIDA, MÁRIO JOSÉ ROMAÑACH. DEPARTMENT OF ORAL DIAGNOSIS, PIRACICABA DENTAL SCHOOL, STATE UNIVERSITY OF CAMPINAS; DEPARTMENT. This study evaluated the clinical, histopathological, and immunohistochemical features of granular cell tumors of the oral cavity. Study Design: Granular cell tumors were retrieved from the files of two oral pathology laboratories in Brazil from 1975 to
ABSTRACTS Abstracts e209 2013. Twenty-five cases were included and subjected to immunohistochemical analysis through the streptavidin-biotin technique. Results: Most patients were in the third decade of life (mean age 36 years), and the lesions were observed mainly in the tongue of females. Microscopically, tumors were composed of clusters of polygonal cells with abundant granular cytoplasm in the connective tissue, which was covered by superficial epithelium with pseudoepitheliomatous hyperplasia. The tumor cells were positive for S-100, CD63, and CD68, and they were negative for CD163 and Claudin-1. Conclusion: Granular cell tumors are uncommon neural tumors that mainly affect the tongue of adult patients. The neural granular cells probably exhibit histiocytic differentiation and are not related to perineural cells.
PE-399 - HISTOPATHOLOGICAL ANALYSIS OF PERIAPICAL GRANULOMA AND RADICULAR CYSTS: A COMPARATIVE STUDY. MARIA ALICE RAMALHO DE SÁ LEITE, RAFAELA ALBUQUERQUE MELO, LUIZ CARLOS ALVES JÚNIOR, BARBARA VANESSA DE BRITO MONTEIRO, THÂMARA MANOELA MARINHO BEZERRA, JOABE DOS SANTOS PEREIRA, MÁRCIA CRISTINA DA COSTA MIGUEL. UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE. Periapical granulomas (PG) and radicular cysts (RC) are common inflammatory periapical lesions whose etiology is associated with the presence of bacteria in root canals. The study investigated and compared the histopathological features of PGs and RCs. Methods: Fifty specimens, including 25 PGs and 25 RCs, were evaluated histopathologically. Results: Analysis of the inflammatory infiltrate in PGs revealed 21 cases (84%) with inflammatory infiltrate grade III, 3 cases (12%) grade II, and 1 case (4%) grade I. Among the RCs, 14 cases (56%) showed inflammatory infiltrate grade III, 8 cases (32%) grade II, and 3 cases (12%) infiltrate grade I. Morphological analysis of the epithelial thickness in RCs revealed the presence of an atrophic epithelium in 14 cases (58%). Conclusion: There were more inflammatory cells in cases of PGs than in cases of RCs, suggesting higher antigenic stimulation in PGs.
PE-400 - HYPOSALIVATION IN NEUROFIBROMATOSIS TYPE 1: A PRELIMINARY STUDY. ELOÁ BORGES LUNA, RAQUEL RICHELIEU LIMA DE ANDRADE PONTES, RAFAELA ELVIRA ROZZA DE MENEZES, ELIANE PEDRA DIAS, MARIA ELISA RANGEL JANINI, KARIN SOARES GONÇALVES CUNHA. UFF. Neurofibromatosis type 1 (NF1) is one of the most common genetic diseases and can affect many organic systems. No studies investigated the glandular function in NF1 individuals. This study evaluated the unstimulated (US) and stimulated salivary flow (SS) in 37 patients with NF1. Material and Methods: Whole saliva was measured between 8:00 and 12:00 AM using the spitting method (5 min). Salivary flow rates were classified as very low (US < 0.1 mL/min; SS < 0.7 mL/min), low (US 0.1 to 0.25 mL/min; SS 0.7 to 1.0 mL/min), and normal (US > 0.25 mL/min; SS >1.0 mL/min). Results: The prevalence of hyposalivation was high (US ¼ 62.1%; SS ¼ 78.4%) in NF1, and the majority presented very low rates (US ¼ 60.8%; SS ¼ 72.4%). There was no association with drugs that cause xerostomia. Hyposalivation is common among individuals with NF1. Conclusion: Other studies are needed to investigate if NF1 is a predisposing disease for hyposalivation or if other factors are associated with hyposalivation in NF1.
PE-397 - GLUT-1 AS A TOOL FOR THE CORRECT DIAGNOSIS OF HEMANGIOMAS: AN UPDATE OF ALL CASES DIAGNOSED AT AN ORAL PATHOLOGY SERVICE. TIAGO JOAO DA SILVA FILHO, DENISE HÉLEN IMACULADA PEREIRA DE OLIVEIRA, LUCIANA ELOÍSA DA SILVA CASTRO, ANDRÉIA FERREIRA DO CARMO, RODRIGO GADELHA VASCONCELOS, PATRÍCIA TEIXEIRA DE OLIVEIRA, LÉLIA MARIA GUEDES QUEIROZ. UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE. This study analyzed the immunohistochemical expression of human glucose transporter proteins (GLUT-1) in all cases diagnosed as hemangiomas from a service of Oral Pathology and reclassified such lesions according their immunoexpression. Study Design: A total of 79 cases histologically diagnosed as hemangiomas were retrieved from a total of 12,100 lesions diagnosed at the Division of Oral Pathology, Federal University of Rio Grande do Norte, between 1970 and 2013. For all those lesions, we assessed the immunohistochemical expression of GLUT-1. Some cases were reclassified as pyogenic granulomas (PG) and vascular malformations (VM). Results: This research showed that only 26 cases initially classified as hemangiomas presented immunopositivity for GLUT-1 and fit the initial diagnosis. The remaining 53 cases were reclassified as PG (20 cases) and VM (33 cases). Conclusion: This study showed that the histological diagnosis alone is not always enough for the correct diagnosis of infantile hemangiomas.
PE-398 - GRANULAR CELL TUMOR: HISTOPATHOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY OF 25 NEW CASES. MARISOL MARTÍNEZ MARTÍNEZ, MARISOL MARTÍNEZ MARTÍNEZ, FELIPE PAIVA FONSECA, THAMYRES CAMPOS FONSECA, MICHELLE AGOSTINI, OSLEI PAES DE ALMEIDA, MÁRIO JOSÉ ROMAÑACH. DEPARTMENT OF ORAL DIAGNOSIS, PIRACICABA DENTAL SCHOOL, STATE UNIVERSITY OF CAMPINAS; DEPARTMENT. This study evaluated the clinical, histopathological, and immunohistochemical features of granular cell tumors of the oral cavity. Study Design: Granular cell tumors were retrieved from the files of two oral pathology laboratories in Brazil from 1975 to
ABSTRACTS Abstracts e209 2013. Twenty-five cases were included and subjected to immunohistochemical analysis through the streptavidin-biotin technique. Results: Most patients were in the third decade of life (mean age 36 years), and the lesions were observed mainly in the tongue of females. Microscopically, tumors were composed of clusters of polygonal cells with abundant granular cytoplasm in the connective tissue, which was covered by superficial epithelium with pseudoepitheliomatous hyperplasia. The tumor cells were positive for S-100, CD63, and CD68, and they were negative for CD163 and Claudin-1. Conclusion: Granular cell tumors are uncommon neural tumors that mainly affect the tongue of adult patients. The neural granular cells probably exhibit histiocytic differentiation and are not related to perineural cells.
PE-399 - HISTOPATHOLOGICAL ANALYSIS OF PERIAPICAL GRANULOMA AND RADICULAR CYSTS: A COMPARATIVE STUDY. MARIA ALICE RAMALHO DE SÁ LEITE, RAFAELA ALBUQUERQUE MELO, LUIZ CARLOS ALVES JÚNIOR, BARBARA VANESSA DE BRITO MONTEIRO, THÂMARA MANOELA MARINHO BEZERRA, JOABE DOS SANTOS PEREIRA, MÁRCIA CRISTINA DA COSTA MIGUEL. UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE. Periapical granulomas (PG) and radicular cysts (RC) are common inflammatory periapical lesions whose etiology is associated with the presence of bacteria in root canals. The study investigated and compared the histopathological features of PGs and RCs. Methods: Fifty specimens, including 25 PGs and 25 RCs, were evaluated histopathologically. Results: Analysis of the inflammatory infiltrate in PGs revealed 21 cases (84%) with inflammatory infiltrate grade III, 3 cases (12%) grade II, and 1 case (4%) grade I. Among the RCs, 14 cases (56%) showed inflammatory infiltrate grade III, 8 cases (32%) grade II, and 3 cases (12%) infiltrate grade I. Morphological analysis of the epithelial thickness in RCs revealed the presence of an atrophic epithelium in 14 cases (58%). Conclusion: There were more inflammatory cells in cases of PGs than in cases of RCs, suggesting higher antigenic stimulation in PGs.
PE-400 - HYPOSALIVATION IN NEUROFIBROMATOSIS TYPE 1: A PRELIMINARY STUDY. ELOÁ BORGES LUNA, RAQUEL RICHELIEU LIMA DE ANDRADE PONTES, RAFAELA ELVIRA ROZZA DE MENEZES, ELIANE PEDRA DIAS, MARIA ELISA RANGEL JANINI, KARIN SOARES GONÇALVES CUNHA. UFF. Neurofibromatosis type 1 (NF1) is one of the most common genetic diseases and can affect many organic systems. No studies investigated the glandular function in NF1 individuals. This study evaluated the unstimulated (US) and stimulated salivary flow (SS) in 37 patients with NF1. Material and Methods: Whole saliva was measured between 8:00 and 12:00 AM using the spitting method (5 min). Salivary flow rates were classified as very low (US < 0.1 mL/min; SS < 0.7 mL/min), low (US 0.1 to 0.25 mL/min; SS 0.7 to 1.0 mL/min), and normal (US > 0.25 mL/min; SS >1.0 mL/min). Results: The prevalence of hyposalivation was high (US ¼ 62.1%; SS ¼ 78.4%) in NF1, and the majority presented very low rates (US ¼ 60.8%; SS ¼ 72.4%). There was no association with drugs that cause xerostomia. Hyposalivation is common among individuals with NF1. Conclusion: Other studies are needed to investigate if NF1 is a predisposing disease for hyposalivation or if other factors are associated with hyposalivation in NF1.