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High fertilization rate with intracytoplasmic sperm injection in mosaic Klinefelter's syndrome
316
Citations from the literature/
fnternational~ Journal of Gynecology & Obstetrics 50 (1995) 315-323
patients of reproductive age (20-30 years) with papilloma virus infection associated with cervical intraepithelial neoplasia (GIN) was carried out. Of these patients, 28 with papilloma virus infection associatedwith CIN(I-II) made up group I and II having this infection associatedwith GIN(I1) made up group 2. Dysbiotic disturbances in vaginal bacterial flora, found in these patients, were manifested by a decrease in the isolation rate and number of the lacto- and bitidobacteria simultaneously with the excessive growth of opportunistic bacteria. The results of the oral administration of Solco-Trichovac are indicative of the effectiveness of this preparation, which was confirmed by the data of clinical and bacteriological studies. Together with an increase in the isolation rate of lacto- and bitidobacteria, the level of the contamination of the cervicovaginal niche with opportunistic and pathogenic bacterial strains decreased.The results thus obtained make it possible to recommend Solco-Trichovac for the complex treatment of papilloma virus infection associated with CIN. Duode~l atresia Doietsky S.Ya.; Arapova A.V.; Zagudaev S.A.; Reznikova A.E. RVS
KHIRURGIYA 1994 70/8 (25-27) The article deals with 5-year (1986-1992) experience in the diagnosis and treatment of duodenal atresia in 26 infants. Timely diagnosis of this developmental anomaly, the variants of operative treatment, and the princiRles of postoperative management are discussed. The necessity of antenatal ultrasonic examination and the high diagnostic value of radiological and endoscopic methods of diagnosis are emphasized. Operations were conducted on 25 children for total high intestinal obstruction, on 23 infants in the neonatal period. The continuity of the intestinal tube was restored by duodenoduodenostomy (12), duodenojejunostomy (3) gastroduodenostomy (1) excision of the membrane (4), and dissection of the membrane (5). The latter is technically the simplest and is the operation of choice. Preliminary jejunostomy for adequate preparation for the radical operation is recommended in profoundly pretenn infants with a serious premorbid background. Of high importance is postoperative enteral catheteral feeding which is begun from the first day after the operation. Seven out of the 26 patients died from severecombined developmental anomalies and infection. The mortality was 26.9%.
FERTILITY
intracytoplasmic sperm injection were obtained from a fresh ejaculate estimated to contain < 100motile sperm. In the first IVF-ICSI attempt, out of seven oocytes that were collected from the wife, four were mature and were injected by ICSI. Fertilization occurred in all four oocytes but only one cleaved and was transferred to the uterus. Pregnancy test was negative 16 days after ET. In the second treatment cycle four out of eight oocyteswere selectedfor ICSI. All four fertilized, three cleaved at the right time, and two were transferred into the wife’s uterus. One embryo was frozen. Pregnancy test 16 days after ET was negative. The high fertilization,rate achieved in this caseindicates the potential of ICSI to treat extreme male infertility. Its use offers hope to those patients with conditions previously considered to be untreatable. Birth after preimplantatioo diagnosis of the cystic fibrosis DeltaFSO8mutation by polymerase chain reaction in humanembryos resultiog from intracytoplasmic epididymal sperm
sperm injection
with
Liu J.; Lisscns W.; Silber S.J.; Devroey P.; Liebaers I.; Van Steirteghem A. EEL
J AM MED ASSOC 1994272/23 (1858-1860) Men with congenital bilateral absence of the vas deferens (CBAVD) have been regarded as presenting a mild form of cystic fibrosis (CF). In this article, we report a case of malefactor infertility, in which both partners are carriers of the DehaF508 mutation and the male partner has CBAVD. Microsurgical epididymal sperm aspiration (MESA) was performed to obtain spermatozoa; intracytoplasmic sperm injection (ICSI) was carried out on the oocytes since the motility of the spermatozoawas severelyimpaired; and embryo biopsy and a polymerase chain reaction (PCR) were carried out for preimplantation diagnosis of the CF DeltaF508 mutation. Singleblastomere analysis was performed and indicated that two embryos were affected (homoxygous DeltaF508) and three embryos were carriers. After transfer of the latter three embryos, a singleton pregnancy was established. At amniocentesis, the DeltaF508 carrier status of the fetus with a 46, XY karyotypc was confirmed. A healthy boy was born and the presence of vasa deferentia, bilaterally, was confirmed. The CF sweat test was also normal. Successful fertilization can be obtained by combination of MESA and ICSI in patients with CBAVD. Preimplantation diagnosis of CF is indicated. Pregnancy and birth of normal children can ensue in such patients. Alleviation of acrosome reactioo prematurity by sperm treatment with egg yolk
AND STERILITY
Tesarik J.; Mendoxa C. High fertilization rate with intracytoplasmic mosaic Klinefelter’s syndrome
sperm injection in
Harari 0.; Boume H.; Baker G.; Gronow M.; Johnston I. AVS
FERTIL STERIL 199563/l (182-184) With the introduction of intracytoplasmic sperm injection (ICSI) as a practical successfultreatment for male infertility, we are able to offer the procedure to a group of patients who probably could never father a child of their own. From a patient with mosaic Khnefelter’s syndrome, sufficient motile sperm for
FRA
FERTIL STERIL 1995 63/l (153-157) Objective: To examine the effects of egg yolk on the spontaneous and ionophore-induced acrosome reaction (AR) and sperm-xona pellucida (ZP) binding in spermatozoa from patients with AR prematurity. Design: Prospective analysis. Setting: Private hospital, public research center, and a university-based laboratory. Patients: In vitro fertilization patients selected on the basis of previous AR testing. Interventions: None. Main Outcome Measures: Frequency of the AR,
Citations from the literature/
fnternational~ Journal of Gynecology & Obstetrics 50 (1995) 315-323
patients of reproductive age (20-30 years) with papilloma virus infection associated with cervical intraepithelial neoplasia (GIN) was carried out. Of these patients, 28 with papilloma virus infection associatedwith CIN(I-II) made up group I and II having this infection associatedwith GIN(I1) made up group 2. Dysbiotic disturbances in vaginal bacterial flora, found in these patients, were manifested by a decrease in the isolation rate and number of the lacto- and bitidobacteria simultaneously with the excessive growth of opportunistic bacteria. The results of the oral administration of Solco-Trichovac are indicative of the effectiveness of this preparation, which was confirmed by the data of clinical and bacteriological studies. Together with an increase in the isolation rate of lacto- and bitidobacteria, the level of the contamination of the cervicovaginal niche with opportunistic and pathogenic bacterial strains decreased.The results thus obtained make it possible to recommend Solco-Trichovac for the complex treatment of papilloma virus infection associated with CIN. Duode~l atresia Doietsky S.Ya.; Arapova A.V.; Zagudaev S.A.; Reznikova A.E. RVS
KHIRURGIYA 1994 70/8 (25-27) The article deals with 5-year (1986-1992) experience in the diagnosis and treatment of duodenal atresia in 26 infants. Timely diagnosis of this developmental anomaly, the variants of operative treatment, and the princiRles of postoperative management are discussed. The necessity of antenatal ultrasonic examination and the high diagnostic value of radiological and endoscopic methods of diagnosis are emphasized. Operations were conducted on 25 children for total high intestinal obstruction, on 23 infants in the neonatal period. The continuity of the intestinal tube was restored by duodenoduodenostomy (12), duodenojejunostomy (3) gastroduodenostomy (1) excision of the membrane (4), and dissection of the membrane (5). The latter is technically the simplest and is the operation of choice. Preliminary jejunostomy for adequate preparation for the radical operation is recommended in profoundly pretenn infants with a serious premorbid background. Of high importance is postoperative enteral catheteral feeding which is begun from the first day after the operation. Seven out of the 26 patients died from severecombined developmental anomalies and infection. The mortality was 26.9%.
FERTILITY
intracytoplasmic sperm injection were obtained from a fresh ejaculate estimated to contain < 100motile sperm. In the first IVF-ICSI attempt, out of seven oocytes that were collected from the wife, four were mature and were injected by ICSI. Fertilization occurred in all four oocytes but only one cleaved and was transferred to the uterus. Pregnancy test was negative 16 days after ET. In the second treatment cycle four out of eight oocyteswere selectedfor ICSI. All four fertilized, three cleaved at the right time, and two were transferred into the wife’s uterus. One embryo was frozen. Pregnancy test 16 days after ET was negative. The high fertilization,rate achieved in this caseindicates the potential of ICSI to treat extreme male infertility. Its use offers hope to those patients with conditions previously considered to be untreatable. Birth after preimplantatioo diagnosis of the cystic fibrosis DeltaFSO8mutation by polymerase chain reaction in humanembryos resultiog from intracytoplasmic epididymal sperm
sperm injection
with
Liu J.; Lisscns W.; Silber S.J.; Devroey P.; Liebaers I.; Van Steirteghem A. EEL
J AM MED ASSOC 1994272/23 (1858-1860) Men with congenital bilateral absence of the vas deferens (CBAVD) have been regarded as presenting a mild form of cystic fibrosis (CF). In this article, we report a case of malefactor infertility, in which both partners are carriers of the DehaF508 mutation and the male partner has CBAVD. Microsurgical epididymal sperm aspiration (MESA) was performed to obtain spermatozoa; intracytoplasmic sperm injection (ICSI) was carried out on the oocytes since the motility of the spermatozoawas severelyimpaired; and embryo biopsy and a polymerase chain reaction (PCR) were carried out for preimplantation diagnosis of the CF DeltaF508 mutation. Singleblastomere analysis was performed and indicated that two embryos were affected (homoxygous DeltaF508) and three embryos were carriers. After transfer of the latter three embryos, a singleton pregnancy was established. At amniocentesis, the DeltaF508 carrier status of the fetus with a 46, XY karyotypc was confirmed. A healthy boy was born and the presence of vasa deferentia, bilaterally, was confirmed. The CF sweat test was also normal. Successful fertilization can be obtained by combination of MESA and ICSI in patients with CBAVD. Preimplantation diagnosis of CF is indicated. Pregnancy and birth of normal children can ensue in such patients. Alleviation of acrosome reactioo prematurity by sperm treatment with egg yolk
AND STERILITY
Tesarik J.; Mendoxa C. High fertilization rate with intracytoplasmic mosaic Klinefelter’s syndrome
sperm injection in
Harari 0.; Boume H.; Baker G.; Gronow M.; Johnston I. AVS
FERTIL STERIL 199563/l (182-184) With the introduction of intracytoplasmic sperm injection (ICSI) as a practical successfultreatment for male infertility, we are able to offer the procedure to a group of patients who probably could never father a child of their own. From a patient with mosaic Khnefelter’s syndrome, sufficient motile sperm for
FRA
FERTIL STERIL 1995 63/l (153-157) Objective: To examine the effects of egg yolk on the spontaneous and ionophore-induced acrosome reaction (AR) and sperm-xona pellucida (ZP) binding in spermatozoa from patients with AR prematurity. Design: Prospective analysis. Setting: Private hospital, public research center, and a university-based laboratory. Patients: In vitro fertilization patients selected on the basis of previous AR testing. Interventions: None. Main Outcome Measures: Frequency of the AR,