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Human malignant melanoma: Significance of chromosomal aberrations
158
Abstracts
14
KARYOTYPIC CHANGES IN THREE HUMAN PRIMARY MALIGNANT MELANOMAS. Jens Jergen Elberg and Mogens R~nne. Institute of Anatomy and Cytology and Department of Plastic Surgery, Odense University Hospital, DK-5000 Odense, Denmark.
Most cytogenetic analyses of malignant melanomas has been done on metastatic lesions or cells from established cell-linas. These studies have displayed multiple chromosomal rearrangements preferentially involving chromosome no. 1,6 and 7 [1]. This paper presents a simple and reliable method for chromosome analyses in primary malignant melanoma based on short-term cultures [2]. Three cases of primary malignant melanoma of the SSM type were found to be hypodiploid. In one of the tumors a deletion of chromosome no. 1 del(1)(pte~-~p34::p21---~qter)was found to be the primary aberration. Further, an identical deletion of chromosome no. 1 (del(1) (ptet~--~q21)was found in two of the three tumors. The involvement of chromosome no. 1 have been considered to be a characteristic of advanced lesions [1]. However, the described early primary lesions may indicate a role of chromosome no. I in the initiation of primary malignant melanomas. The oncogene N-ras located at lp22 may be significant in the carcinogenic process. REFERENCES 1. Limon J, et al. (1988): Chromosome changes in metastatic human melanoma. Cancer Genet Cytogenet 30:201-211. 2. R~nne M, Elberg JJ (1987): Banding and preparational techniques in normal and neoplastic tissue. I1. Short-term cultures from human solid tumors. Anticancer Res 7:1209-1211.
15
CYTOGENETIC STUDY OF INTRAOCULAR MELANOMAS. G. Prescher, 1 R. Becher,z and N. Bornfeld.s 1West German Tumor Center, Departments of Oncology and ZOphthalmology, Universititsklinikum Essen, Hufelandstr, 55, 4300 Essen 1, FRG.
In twelve cases, directly after enucleation tumor samples of primary choroidel melanomas were mechanically and enzymatically disa~regated and short term cultures set up for cytogenetic analysis. A sufficient number of banded metaphases was obtained in seven cases. Between ten and twenty karyotypes were analysed, The constitutional karyotype was also established in these patients and found to be normal in all patients, Clonal chromosomal anomalies in tumor cells were detected in six of seven cases. In three cases a loss of one sex chromosome (-Y in two male patients and - X in one female patient) was observed which was restricted to tumor cells. In one case this was the only atypical finding. Monosomy 3 was found in two other cases, associated with trisomy 8 in one and an isochromosome 8q in the second patient, Structural anomalies involving breakpoints on chromosome lp wets observed in two cases and anomalies of chromosome 6 in two cases. A clonal 20p+ and a 5q+ anomaly was detected in one case each. Constitutional heterechromatin polymorphism of chromosome I (lqh+) was seen in one patient without further anomalies in the tumor cells. Our preliminary conclusions are that monosr~my 3, trisomy 8 or 8q and structural anomalies of chromosome I and 6 occur nonrandom in primary choroidal melanomas.
16
HUMAN MALIGNANT MELANOMA: SIGNIFICANCE OF CHROMOSOMAL ABERRATIONS. P. Grammatico,! M. L. Lo Re, ! C. Barletta,2 C. Peschler,2 S. Scarps, i A, Modesti,! G. Del Porto. ~ ~Dipartimento di Medicina Sparimentale, Universit& degli Studi di Rams "La Sapienza," 2DiparUmanto di Ematologia ed Oncologia, Istituto Superiore di SanitY, Rams, Italy.
In literature there are a lot of works about t:ytoganetic studies of human malisnant melanoma (HMM) but it is till now impossible to define the significance of different markers reported. In fact we think that the difficul. ties in interpretating the chromosomal aberrations is due to a not well defined clinical condition and its correlation with cytological and histological analysis. So we present several cases of HMM well defined for: clinical, cytological, histological, cytogenetic and molecular aspects. The authors discuss the cytosenetic results comparing them with all the parameters tested and stress the importance of their correlation.
Abstracts
14
KARYOTYPIC CHANGES IN THREE HUMAN PRIMARY MALIGNANT MELANOMAS. Jens Jergen Elberg and Mogens R~nne. Institute of Anatomy and Cytology and Department of Plastic Surgery, Odense University Hospital, DK-5000 Odense, Denmark.
Most cytogenetic analyses of malignant melanomas has been done on metastatic lesions or cells from established cell-linas. These studies have displayed multiple chromosomal rearrangements preferentially involving chromosome no. 1,6 and 7 [1]. This paper presents a simple and reliable method for chromosome analyses in primary malignant melanoma based on short-term cultures [2]. Three cases of primary malignant melanoma of the SSM type were found to be hypodiploid. In one of the tumors a deletion of chromosome no. 1 del(1)(pte~-~p34::p21---~qter)was found to be the primary aberration. Further, an identical deletion of chromosome no. 1 (del(1) (ptet~--~q21)was found in two of the three tumors. The involvement of chromosome no. 1 have been considered to be a characteristic of advanced lesions [1]. However, the described early primary lesions may indicate a role of chromosome no. I in the initiation of primary malignant melanomas. The oncogene N-ras located at lp22 may be significant in the carcinogenic process. REFERENCES 1. Limon J, et al. (1988): Chromosome changes in metastatic human melanoma. Cancer Genet Cytogenet 30:201-211. 2. R~nne M, Elberg JJ (1987): Banding and preparational techniques in normal and neoplastic tissue. I1. Short-term cultures from human solid tumors. Anticancer Res 7:1209-1211.
15
CYTOGENETIC STUDY OF INTRAOCULAR MELANOMAS. G. Prescher, 1 R. Becher,z and N. Bornfeld.s 1West German Tumor Center, Departments of Oncology and ZOphthalmology, Universititsklinikum Essen, Hufelandstr, 55, 4300 Essen 1, FRG.
In twelve cases, directly after enucleation tumor samples of primary choroidel melanomas were mechanically and enzymatically disa~regated and short term cultures set up for cytogenetic analysis. A sufficient number of banded metaphases was obtained in seven cases. Between ten and twenty karyotypes were analysed, The constitutional karyotype was also established in these patients and found to be normal in all patients, Clonal chromosomal anomalies in tumor cells were detected in six of seven cases. In three cases a loss of one sex chromosome (-Y in two male patients and - X in one female patient) was observed which was restricted to tumor cells. In one case this was the only atypical finding. Monosomy 3 was found in two other cases, associated with trisomy 8 in one and an isochromosome 8q in the second patient, Structural anomalies involving breakpoints on chromosome lp wets observed in two cases and anomalies of chromosome 6 in two cases. A clonal 20p+ and a 5q+ anomaly was detected in one case each. Constitutional heterechromatin polymorphism of chromosome I (lqh+) was seen in one patient without further anomalies in the tumor cells. Our preliminary conclusions are that monosr~my 3, trisomy 8 or 8q and structural anomalies of chromosome I and 6 occur nonrandom in primary choroidal melanomas.
16
HUMAN MALIGNANT MELANOMA: SIGNIFICANCE OF CHROMOSOMAL ABERRATIONS. P. Grammatico,! M. L. Lo Re, ! C. Barletta,2 C. Peschler,2 S. Scarps, i A, Modesti,! G. Del Porto. ~ ~Dipartimento di Medicina Sparimentale, Universit& degli Studi di Rams "La Sapienza," 2DiparUmanto di Ematologia ed Oncologia, Istituto Superiore di SanitY, Rams, Italy.
In literature there are a lot of works about t:ytoganetic studies of human malisnant melanoma (HMM) but it is till now impossible to define the significance of different markers reported. In fact we think that the difficul. ties in interpretating the chromosomal aberrations is due to a not well defined clinical condition and its correlation with cytological and histological analysis. So we present several cases of HMM well defined for: clinical, cytological, histological, cytogenetic and molecular aspects. The authors discuss the cytosenetic results comparing them with all the parameters tested and stress the importance of their correlation.