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Hyaluronan (HA) is not a usable serum marker for human osteoarthritis (OA)
Osteoarthritis and Cartilage Vol. 1 No. 1 dose may play an important causative role in the failure of the repair processes and therefore we measured pyridinoline crosslinks. Twenty-two adolescent male NZW rabbits were injected into the left knee joint with a single dose of either 0.2 mg or 2.0 mg of CP. Blood and urine samples were taken before and at different time points after the injection. Serum KS level was measured by an ELISA. Pyridinium crosslinks, pyridinoline (Pyr) and deoxypyridinoline (D-Pyr) were measured in urine by a very sensitive HPLC assay using post-column fluorescence detection. Rabbits injected with both doses of CP showed an increase in Pyr as early as 24 h after injection. On day 2, it was further increased in both low- (103%) and highdose (220%) groups. Pyr returned to below baseline values after 1 month in both groups. D-Pyr, the bone specific crosslink, was significantly increased in the
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high-dose group after 24 h (216%) and 48 h (226%) but not in the low-dose group (39% and 21% respectively). D-Pyr reached its maximum in the high-dose group on day 9 (492%). In the low-dose group, D-Pyr reached baseline pre-injection values by day 12. In the high-dose group, levels rose again at later times before dropping to stable values 3 months after CP injection. These results suggests disorganization and/or degradation of the collagen network of articular cartilage which may be responsible, in part, for the degenerative changes that are reproducibly Observed in weightbearing regions of the articular surface of animals injected with 2.0 mg CP. The observation t h a t the injection also appears to lead to a marked increase in the rate of urinary excretion of the D-Pyr crosslink found only in bone suggests that bone becomes integrally involved in the response of the joint to the injury in this model.
A s t u d y o f s e r u m p h o s p h o l i p a s e A2 (PLA2) a c t i v i t y as a m a r k e r o f h u m a n o s t e o a r t h r i t i s (OA) J. C. BALBLANC, T. CONROZIER, P. MATHIEU, P. BROQUET, M. RICHARD AND E. VIGNON
Claud Bernard University, H6pital Edouard Herriot, Lyon, France PLA2 activity has been determined in the serum of 67 OA patients and 17 normal controls using a specific radiolabeled substrate. Activity of the enzyme was found to be unaffected by the duration of serum storage at -80°C (over a 77-month period). PLA2 activity was significantly higher in OA patients than in controls (102_ 70 and 45__+25 dpm/h/ml respectively, P = 0.002). The Lequesne index of OA severity, age, disease duration, morning stiffness, OA radiological severity, erythrocyte sedimentation rate and C reactive protein were not related to serum PLA2 activity in 43 patients.
Changes in Lequesne index on a 46-day follow-up study of 12 OA patients were unrelated with changes in PLA2 activity. Radiological changes in 14 patients with hip or knee OA over a mean follow up study of 21.7 months were assessed blindly. An increase in PLA2 activity was only observed in patients showing narrowing of the joint space. The results suggest that an increase in serum PLA2 activity in OA patient could be related to progression of cartilage destruction lesions.
H y a l u r o n a n (HA) is n o t a u s a b l e s e r u m m a r k e r f o r h u m a n o s t e o a r t h r i t i s (OA) J. C. BALBLANC, D. HARTMANN, P. MATHIEU, T. CONROZIER, M. RICHARD AND E. VIGNON
Claude Bernard University, Hdpital Edouard Herriot, Lyon, France HA has been determined in the serum of 85 OA patients and 39 normal controls u s i n g a radiometric assay (PHARMACIA). Each serum sample was collected early in the morning to avoid diurnal changes in HA. HA was higher in OA patients than in controls (101_+84 and 39__+21 pg/1 respectively, P = 0.0001). However, HA was found to be unrelated with the Lequesne index of OA severity, disease duration, morning stiffness, number of
OA affected joints, radiological stage of OA severity, as well as ESR and CRP. Changes in HA and Lequesne inde~,,determined in 30 Patients over a 62-day follow-up study were unrelated. Changes in HA and radiographic findings, assessed blindly in 17 OA patients over a mean follow-up study of 22 months were also unrelated. Serum HA does not appear to be a usable marker of OA.
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high-dose group after 24 h (216%) and 48 h (226%) but not in the low-dose group (39% and 21% respectively). D-Pyr reached its maximum in the high-dose group on day 9 (492%). In the low-dose group, D-Pyr reached baseline pre-injection values by day 12. In the high-dose group, levels rose again at later times before dropping to stable values 3 months after CP injection. These results suggests disorganization and/or degradation of the collagen network of articular cartilage which may be responsible, in part, for the degenerative changes that are reproducibly Observed in weightbearing regions of the articular surface of animals injected with 2.0 mg CP. The observation t h a t the injection also appears to lead to a marked increase in the rate of urinary excretion of the D-Pyr crosslink found only in bone suggests that bone becomes integrally involved in the response of the joint to the injury in this model.
A s t u d y o f s e r u m p h o s p h o l i p a s e A2 (PLA2) a c t i v i t y as a m a r k e r o f h u m a n o s t e o a r t h r i t i s (OA) J. C. BALBLANC, T. CONROZIER, P. MATHIEU, P. BROQUET, M. RICHARD AND E. VIGNON
Claud Bernard University, H6pital Edouard Herriot, Lyon, France PLA2 activity has been determined in the serum of 67 OA patients and 17 normal controls using a specific radiolabeled substrate. Activity of the enzyme was found to be unaffected by the duration of serum storage at -80°C (over a 77-month period). PLA2 activity was significantly higher in OA patients than in controls (102_ 70 and 45__+25 dpm/h/ml respectively, P = 0.002). The Lequesne index of OA severity, age, disease duration, morning stiffness, OA radiological severity, erythrocyte sedimentation rate and C reactive protein were not related to serum PLA2 activity in 43 patients.
Changes in Lequesne index on a 46-day follow-up study of 12 OA patients were unrelated with changes in PLA2 activity. Radiological changes in 14 patients with hip or knee OA over a mean follow up study of 21.7 months were assessed blindly. An increase in PLA2 activity was only observed in patients showing narrowing of the joint space. The results suggest that an increase in serum PLA2 activity in OA patient could be related to progression of cartilage destruction lesions.
H y a l u r o n a n (HA) is n o t a u s a b l e s e r u m m a r k e r f o r h u m a n o s t e o a r t h r i t i s (OA) J. C. BALBLANC, D. HARTMANN, P. MATHIEU, T. CONROZIER, M. RICHARD AND E. VIGNON
Claude Bernard University, Hdpital Edouard Herriot, Lyon, France HA has been determined in the serum of 85 OA patients and 39 normal controls u s i n g a radiometric assay (PHARMACIA). Each serum sample was collected early in the morning to avoid diurnal changes in HA. HA was higher in OA patients than in controls (101_+84 and 39__+21 pg/1 respectively, P = 0.0001). However, HA was found to be unrelated with the Lequesne index of OA severity, disease duration, morning stiffness, number of
OA affected joints, radiological stage of OA severity, as well as ESR and CRP. Changes in HA and Lequesne inde~,,determined in 30 Patients over a 62-day follow-up study were unrelated. Changes in HA and radiographic findings, assessed blindly in 17 OA patients over a mean follow-up study of 22 months were also unrelated. Serum HA does not appear to be a usable marker of OA.