Hydroxyethylstarch deposits in skin and other organs

Poster Presentations

582 A CONCERN OFEPIDERMAL LANGERHANS CELLAND LATEX BEADS. Mamoru Masutani,Dep*rtmentof Dermatology,Fujita Health

university School of Medicine, Aichi, Japan When horseradish peroxidase(HRP) and ferritin(F) were inoculated as well as tuberculin test into the back of skin with guinea pigs, epidermal Langerbans cell(K) could be take” up those materials though Langerbans cell granule(LCG) which was made by own cell membrane. Therefore, it is consideration that LCG comes finally to be secondary lyso~ome ~pba~olysosome). ” e have bee” observed the various epidermal cell activity with great and small sizes of latex beads(LBf (0.109 and 0.794um) which has “o protein within only foreign body. This time, it will be described the evidence. A great part of LB was uptake into the dermal phagocyte(PC) a” the observation. the", PC went up to the epidermis tbrougbout a basal lamina. LB different from HRP and F can “at directly take by LCG in LC. However, foreign body wlthin pbag”cyte in the epidermal region was a target for the LC. It is considered as one of the immunolagical ability of LC. I” the above consequence, epidermal LC was meant antigen presenting cell.

And

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580 DISTRIBUTION OF 4-HYDROXY-2-NONENAL-MODIFIED PROTEIN IN ki”zl EXPERIMENTAL BURN AND HUMAN SKIN TUMOR. Yukari N&k” Chikak” Nishinori”. Shinva Tovokuni”. Koii Uchida”, Hiroshi Hu.in, and Sadao ~am”ra”. Depanment of Dermatology” and Palhology”, Kyol” Unwersily Faculty of Medicine, Kyot”, Japan. Deptiment of Applied Biological Science”, Nagoya University School of AgricuIIure. Nagoya, Japan. 4.Hydroxy-2.nonenal peroxidadon.

(HNE)

is one of the major

gen”t”xiciIy

and mutagenicity

in several systems have been shown

authors

(K I..) has demonstrated

fhaf histidine (His) residues

targets

for the modlficatlon

HNE,

HNE-His

adduct

HNE-modihrd (130-I%

of membrane

the

by we

in expenmenral

in several

and prepared

of this

kinds

aotlbody.

bum model

of human

Recently.

of proteins

a polyclonal we

studled

antibody

againsl

Ibe dislnbullon

using 6wk-old

skin tumors

one of the

are important

such

of

male WisL~r rats as squamous

ceU

malignant melanoma and Paget’s disease. One and 3 hrs after bum. posillvc were demonstrated in the cytoplasm

mflammarory

celk.

smce degeneralion after hum

By

protein

g) and

carcinoma. reacfi”ns

producls

A number of adverse biological effects agamst protein and DNA mcludmg

suggesrmg

of folhcular epithebal cells and mfdtraling

tha! HNE-modified

“f foUicu1.v epahebumbecame

Among

human skin wnors

cells, hur non in squamous cell carcinoma large Pagc~ ceils. showed cyloplasmic

studed.

protein

remarkable

worked

posmve reactions

and melanoma

for

not immcdntely.

cytoloxicny but 3 hrs

were seen in Pagrt

cells. Paget

cells. especially

pouiive reaction in 10 of 14 specimens

584