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Imaging fibrosis in pancreatic cancer using second harmonic generation
Pancreatology 15 (2015) 200e201
Contents lists available at ScienceDirect
Pancreatology journal homepage: www.elsevier.com/locate/pan
IMAGES IN PANCREATOLOGY Imaging fibrosis in pancreatic cancer using second harmonic generation Claire Vennin*, Marina Pajic, Paul Timpson Cancer Department, The Kinghorn Cancer Center & The Garvan Institute, 384 Victoria Road 2010, Darlinghurst, New South Wales, Australia
Legend: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive disease with a dire prognosis. The median survival of patients with advanced pancreatic cancer remains less than 6 months due to surgically unresectable, highly chemoresistant and locally or advanced metastatic disease being present at the time of diagnosis. Consequently, there is a requirement to develop more effective strategies in order to understand the mechanisms underlying chemoresistance and to treat this disease. In PDAC, the tumour-associated stroma undergoes a fibrotic reaction and is characterised by poor vasculature and extensive deposition of extracellular matrix (ECM) components such as collagen I. It has been demonstrated that the resulting dense stroma supports cancer progression and metastasis. In addition, it is suggested
* Corresponding author. E-mail addresses: [email protected] (C. Vennin), [email protected] (M. Pajic), [email protected] (P. Timpson). 1424-3903/$ e see front matter http://dx.doi.org/10.1016/j.pan.2015.02.004
C. Vennin et al. / Pancreatology 15 (2015) 200e201
201
that it represents a barrier against the delivery of chemotherapeutic agents. We used Second Harmonic Generation (SHG) imaging to study collagen I abundance in a patient-derived xenograft (PDX) model of PDAC. This state-of-the art technology allowed us to characterise fibrosis in PDX and correlate this with patient prognosis and outcomes. SHG imaging (purple) was acquired with a multiphoton Leica-SP8 (890 nm) which reads out fibrillar (cross linked, organised) ECM collagen bundles while autofluorescence (890 nm) provides context and outline of tumours in relationship to surrounding matrix interaction.
Acknowledgement:
UNSW Australia NHMRC The Garvan Institute of Medical Research Cancer Council
Contents lists available at ScienceDirect
Pancreatology journal homepage: www.elsevier.com/locate/pan
IMAGES IN PANCREATOLOGY Imaging fibrosis in pancreatic cancer using second harmonic generation Claire Vennin*, Marina Pajic, Paul Timpson Cancer Department, The Kinghorn Cancer Center & The Garvan Institute, 384 Victoria Road 2010, Darlinghurst, New South Wales, Australia
Legend: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive disease with a dire prognosis. The median survival of patients with advanced pancreatic cancer remains less than 6 months due to surgically unresectable, highly chemoresistant and locally or advanced metastatic disease being present at the time of diagnosis. Consequently, there is a requirement to develop more effective strategies in order to understand the mechanisms underlying chemoresistance and to treat this disease. In PDAC, the tumour-associated stroma undergoes a fibrotic reaction and is characterised by poor vasculature and extensive deposition of extracellular matrix (ECM) components such as collagen I. It has been demonstrated that the resulting dense stroma supports cancer progression and metastasis. In addition, it is suggested
* Corresponding author. E-mail addresses: [email protected] (C. Vennin), [email protected] (M. Pajic), [email protected] (P. Timpson). 1424-3903/$ e see front matter http://dx.doi.org/10.1016/j.pan.2015.02.004
C. Vennin et al. / Pancreatology 15 (2015) 200e201
201
that it represents a barrier against the delivery of chemotherapeutic agents. We used Second Harmonic Generation (SHG) imaging to study collagen I abundance in a patient-derived xenograft (PDX) model of PDAC. This state-of-the art technology allowed us to characterise fibrosis in PDX and correlate this with patient prognosis and outcomes. SHG imaging (purple) was acquired with a multiphoton Leica-SP8 (890 nm) which reads out fibrillar (cross linked, organised) ECM collagen bundles while autofluorescence (890 nm) provides context and outline of tumours in relationship to surrounding matrix interaction.
Acknowledgement:
UNSW Australia NHMRC The Garvan Institute of Medical Research Cancer Council