- Email: [email protected]
Immunohistochemical Expression of Cytokeratins in Central and Peripheral Odontogenic Fibromas
ORAL AND MAXILLOFACIAL PATHOLOGY e212 Abstracts PE-410 - IMMUNOHISTOCHEMICAL EXPRESSION OF CYTOKERATINS IN CENTRAL AND PERIPHERAL ODONTOGENIC FIBROMAS. BRUNNO SANTOS DE FREITAS SILVA, KARIN SÁ FERNANDES, MARIA FÁTIMA GUARIZO KLINGBEIL, FERNANDA PAULA YAMAMOTOSILVA, DÉCIO DOS SANTOS PINTO-JÚNIOR. CURSO DE ODONTOLOGIA DO CENTRO UNIVERSITÁRIO DE ANÁPOLIS/GO. This study investigated the immunohistochemical expression of cytokeratins (CK) 5/6, 7, 8, 10, 13, 14, 18, and 19 in five cases of central odontogenic fibromas (COF), nine peripheral odontogenic fibromas (POF), and three dental follicle specimens. Study Design: Paraffin-embedded sections were obtained for the immunohistochemical evaluation of CK monoclonal antibodies. Results: CK 14 expression was positive in the epithelial islands of all cases. In four OF cases (28.57%) a weak positivity to CK 19 was observed in some central stellate cells (three POF and one COF). Of these four cases, three were WHO type and one simple type. Strong expression of CK 7 was detected in some central stellate cells and peripheral columnar cells of three cases of POF (21.43%). Conclusion: CK expression in OFs suggested a variable degree of epithelium maturation, demonstrating a possible difference in COF and POF origin.
PE-411 - IMMUNOHISTOCHEMICAL EXPRESSION OF LANGERHANS CELLS IN ACTINIC CHEILITIS AND LOWER LIP SQUAMOUS CELL CARCINOMA. MARIANNE DE VASCONCELOS CARVALHO, FELIPE PAIVA FONSECA, ROGÉRIO GONDAK, JULIANE OLIVEIRA GOMES, MÁRCIO AJUDARTE LOPES, PABLO AGUSTIN VARGAS. FOP/UNICAMP. Oral mucosal Langerhans cells (LCs) are involved in immunosurveillance against neoantigens associated with malignant transformation. This study compared the presence and distribution of LCs in actinic cheilitis (AC) with/without dysplasia and correlated findings with those in lower lip squamous cell carcinoma (LLSCC). Study Design: Forty-eight cases of AC and 21 of LLSCC were retrieved from the archives of the Department of Oral Diagnosis (Pathology) of the State University of Campinas (Piracicaba Dental School, Brazil). Immunohistochemical analysis was performed with antibodies anti-CD83 and antiCD1a. The quantification of LCs was performed and the results expressed as positive cells per area of epithelium. Results: Increased grade of dysplasia was accompanied by a decrease in the population of LCs and a depletion of these cells in LLSCC samples (p < 0.001). Conclusion: Depletion of LC in lip epithelium may represent a higher risk for malignant transformation of oral lesions such as ACs.
PE-412 - IMMUNOHISTOCHEMICAL PROFILE OF ORAL BENIGN PERIPHERAL NERVE SHEATH TUMORS. GARDÊNIA MATOS PARAGUASSÚ, LUANA COSTA BASTOS, LEONARDO PROVEDEL, CLARISSA ARAÚJO SILVA GURGEL, LUCIANA MARIA PEDREIRA RAMALHO, JEAN NUNES DOS SANTOS, FLÁVIA CALÓ AQUINO XAVIER. UNIVERSIDADE FEDERAL DA BAHIA. Schwannoma and neurofibroma are rare benign peripheral nerve sheath tumors that can be difficult to diagnose based on histopathological analysis. Immunohistochemical analysis may
OOOO February 2014 facilitate diagnosis. This study evaluated the histopathological and immunohistochemical features of oral schwannomas and neurofibromas diagnosed in an oral pathology service between 2002 and 2012. Two schwannomas and three neurofibromas were selected and reviewed from a total of 2,114 oral lesion cases. Immunohistochemical tests included anti-S100, anti-vimentin, anti-VEGF, anti-CD-34, and anti-tryptase antibodies. All tumors demonstrated positive immunostaining for S-100 and showed variable immunostaining intensity for vimentin and VEGF in tumors. CD-34 immunostaining revealed more blood vessels in schwannomas (13.2 0.57) compared to neurofibromas (12.54 0.5). Tryptase immunostaining revealed more mast cells in neurofibromas (5.6 5.44) than in schwannoma (3 4.24). Therefore vascular endothelial factors and mast cells may contribute to the diagnosis of these tumors and have implications for their pathogenesis.
PE-413 - IMMUNOHISTOCHEMICAL STUDY OF CYCLIN D1, COX-2, AND PCNA IN PLEOMORPHIC ADENOMAS. JEFFERSON DA ROCHA TENÓRIO, LEORIK PEREIRA DA SILVA, GEORGE JOÃO FERREIRA DO NASCIMENTO, GLEICY FÁTIMA MEDEIROS DE SOUZA, ANA PAULA VERAS SOBRAL. FACULDADE DE ODONTOLOGIA DE PERNAMBUCO - UNIVERSIDADE DE PERNAMBUCO. Several studies have associated cell cycle proteins and cyclooxygenase-2 (Cox-2) with an increase of neoplastic cell proliferation levels. This work evaluated immunohistochemical expression of cyclin D1 (CD1), Cox-2, and proliferating cell nuclear antigen (PCNA) in pleomorphic adenomas (PAs). Materials and Methods: Clinicopathological data and 3-mm-thick sections of 48 PAs were retrieved from files of the Laboratory of Oral Pathology, Dental School, University of Pernambuco, Camaragibe, Brazil. Anti-CD1, -Cox-2 and -PCNA monoclonal antibodies were used in immunohistochemical staining. Results: Immunoexpression of CD1, PCNA, and Cox2 exhibited positivity in, respectively, 57.9%, 54.2% and 43.8% of PAs (p > 0.05). No statistical associations were found between the proteins studied and the clinicopathological data. Results appear to show that cell proliferation is characteristic of the neoplastic behavior of PAs. Conclusion: Cell cycle and proliferation regulatory proteins (CD1 and Cox-2) are involved in the neoplastic growth of PAs independently of their clinicopathological features.
PE-414 - IMPACT OF PAIN ON QUALITY OF LIFE IN HEAD AND NECK CANCER. SANDRA LÚCIA VENTORIN VON ZEIDLER, MARIANE GARCIA OLIVEIRA, MARCELLA SOL, KARINE GADIOLI OLIVEIRA, JOSE ROBERTO VASCONCELOS PODESTÁ, NAZARÉ SOUZA BISSOLI, SONIA ALVES GOUVEA. FEDERAL UNIVERSITY OF ESPÍRITO SANTO, VITORIA, ES. This study evaluated the quality of life (QoL) and pain levels in patients with head and neck squamous cell carcinoma (HNSCC). Materials and Methods: Interviews were conducted of 127 patients with HNSCC. Pain severity was measured using the Brief Pain Inventory (BPI) questionnaire, and QoL was assessed with the EORTC QLQ-C30 and the QLQ-H&N35 module. Data were analyzed using SPSS software and the Chisquare test (p < 0.05). Results: Mean patient age was 57.9 years, and there was a predominance of males. Most cases
PE-411 - IMMUNOHISTOCHEMICAL EXPRESSION OF LANGERHANS CELLS IN ACTINIC CHEILITIS AND LOWER LIP SQUAMOUS CELL CARCINOMA. MARIANNE DE VASCONCELOS CARVALHO, FELIPE PAIVA FONSECA, ROGÉRIO GONDAK, JULIANE OLIVEIRA GOMES, MÁRCIO AJUDARTE LOPES, PABLO AGUSTIN VARGAS. FOP/UNICAMP. Oral mucosal Langerhans cells (LCs) are involved in immunosurveillance against neoantigens associated with malignant transformation. This study compared the presence and distribution of LCs in actinic cheilitis (AC) with/without dysplasia and correlated findings with those in lower lip squamous cell carcinoma (LLSCC). Study Design: Forty-eight cases of AC and 21 of LLSCC were retrieved from the archives of the Department of Oral Diagnosis (Pathology) of the State University of Campinas (Piracicaba Dental School, Brazil). Immunohistochemical analysis was performed with antibodies anti-CD83 and antiCD1a. The quantification of LCs was performed and the results expressed as positive cells per area of epithelium. Results: Increased grade of dysplasia was accompanied by a decrease in the population of LCs and a depletion of these cells in LLSCC samples (p < 0.001). Conclusion: Depletion of LC in lip epithelium may represent a higher risk for malignant transformation of oral lesions such as ACs.
PE-412 - IMMUNOHISTOCHEMICAL PROFILE OF ORAL BENIGN PERIPHERAL NERVE SHEATH TUMORS. GARDÊNIA MATOS PARAGUASSÚ, LUANA COSTA BASTOS, LEONARDO PROVEDEL, CLARISSA ARAÚJO SILVA GURGEL, LUCIANA MARIA PEDREIRA RAMALHO, JEAN NUNES DOS SANTOS, FLÁVIA CALÓ AQUINO XAVIER. UNIVERSIDADE FEDERAL DA BAHIA. Schwannoma and neurofibroma are rare benign peripheral nerve sheath tumors that can be difficult to diagnose based on histopathological analysis. Immunohistochemical analysis may
OOOO February 2014 facilitate diagnosis. This study evaluated the histopathological and immunohistochemical features of oral schwannomas and neurofibromas diagnosed in an oral pathology service between 2002 and 2012. Two schwannomas and three neurofibromas were selected and reviewed from a total of 2,114 oral lesion cases. Immunohistochemical tests included anti-S100, anti-vimentin, anti-VEGF, anti-CD-34, and anti-tryptase antibodies. All tumors demonstrated positive immunostaining for S-100 and showed variable immunostaining intensity for vimentin and VEGF in tumors. CD-34 immunostaining revealed more blood vessels in schwannomas (13.2 0.57) compared to neurofibromas (12.54 0.5). Tryptase immunostaining revealed more mast cells in neurofibromas (5.6 5.44) than in schwannoma (3 4.24). Therefore vascular endothelial factors and mast cells may contribute to the diagnosis of these tumors and have implications for their pathogenesis.
PE-413 - IMMUNOHISTOCHEMICAL STUDY OF CYCLIN D1, COX-2, AND PCNA IN PLEOMORPHIC ADENOMAS. JEFFERSON DA ROCHA TENÓRIO, LEORIK PEREIRA DA SILVA, GEORGE JOÃO FERREIRA DO NASCIMENTO, GLEICY FÁTIMA MEDEIROS DE SOUZA, ANA PAULA VERAS SOBRAL. FACULDADE DE ODONTOLOGIA DE PERNAMBUCO - UNIVERSIDADE DE PERNAMBUCO. Several studies have associated cell cycle proteins and cyclooxygenase-2 (Cox-2) with an increase of neoplastic cell proliferation levels. This work evaluated immunohistochemical expression of cyclin D1 (CD1), Cox-2, and proliferating cell nuclear antigen (PCNA) in pleomorphic adenomas (PAs). Materials and Methods: Clinicopathological data and 3-mm-thick sections of 48 PAs were retrieved from files of the Laboratory of Oral Pathology, Dental School, University of Pernambuco, Camaragibe, Brazil. Anti-CD1, -Cox-2 and -PCNA monoclonal antibodies were used in immunohistochemical staining. Results: Immunoexpression of CD1, PCNA, and Cox2 exhibited positivity in, respectively, 57.9%, 54.2% and 43.8% of PAs (p > 0.05). No statistical associations were found between the proteins studied and the clinicopathological data. Results appear to show that cell proliferation is characteristic of the neoplastic behavior of PAs. Conclusion: Cell cycle and proliferation regulatory proteins (CD1 and Cox-2) are involved in the neoplastic growth of PAs independently of their clinicopathological features.
PE-414 - IMPACT OF PAIN ON QUALITY OF LIFE IN HEAD AND NECK CANCER. SANDRA LÚCIA VENTORIN VON ZEIDLER, MARIANE GARCIA OLIVEIRA, MARCELLA SOL, KARINE GADIOLI OLIVEIRA, JOSE ROBERTO VASCONCELOS PODESTÁ, NAZARÉ SOUZA BISSOLI, SONIA ALVES GOUVEA. FEDERAL UNIVERSITY OF ESPÍRITO SANTO, VITORIA, ES. This study evaluated the quality of life (QoL) and pain levels in patients with head and neck squamous cell carcinoma (HNSCC). Materials and Methods: Interviews were conducted of 127 patients with HNSCC. Pain severity was measured using the Brief Pain Inventory (BPI) questionnaire, and QoL was assessed with the EORTC QLQ-C30 and the QLQ-H&N35 module. Data were analyzed using SPSS software and the Chisquare test (p < 0.05). Results: Mean patient age was 57.9 years, and there was a predominance of males. Most cases