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Influence of capsaicin on anaphylactic reaction in the guinea-pig
17
Pharmaco~g~alResearch Commun~ation¢VoL 2~ Supp~mentL 1988 INFLUENCE OF CAPSAICIN ON ANAPHYLACTIC REACTION tN THE GUINEA-PIG S. Franchi
Micheli,
G, Genti l i n i ,
Department o f P r e c l i n i c a l
Key words: h i s t a m i n e ~
due
capsaicin~
is known t h a t
Pharmacology - U n i v e r s i t y
On
the other
including
different
modulatory
role
are
is m o d u l a t e d by a
neurotransmitters. to
be i n v o l v e d
bronchial
of Florence
Since
in
asthma.
effect
and t h i s
o£ n e u r o p e p t i d e s
in neurogenic i n f l a m m a t i o n
t987),
ileum,
neurotransmission
depletion
hand a n a p h y l a x i s
believed
(Barnes~
anaphylaxis,
oapsaicin affects
in p a r t to a r e l e a s e a n d / o r
1986),
they
and C l i n i c a l
S. L u z z i and L. Z i l l e t t i
Morgagni, 65 - 5 0 | 3 4 F l o r e n c e - t e l 055/411133-431364.
V. le G.B,
It
H. C i u f f i ,
(Buck and BurKs,
complex
mechanism
neuropeptides
exert
(S~ria and Lundberg,
pathogenesis
we wanted t o assess t h e e v e n t u a l
of
is
and
1985)
bronchial
interference
a
asthma
of capsaicin
on anaphylaxis.
(0^)
Hale
guinea-pigs
( 3 0 0 - 4 0 0 g) were a c t i v e l y
i,p,
and used f o r
e x p e r i m e n t s 3-4 weeks l a t e r ,
anaphylactic
sensitized
microshocK was p e r f o r m e d by e v a l u a t i n g an-imals exposed t o t h e a n t i g e n a e r o s o l the drug was i n j e c t e d
c a p s a i c i n was used, vitro
experiments,
Zar,
t968)
were
isolated
histamine
fluid
released
performed the
for
i.p,
3 min;
ovalbumin
In in v i v o
experiments
the preconvulsion (Herxheimer,
time
t952)o
Krebs
the perfusion
muscle
Henseleit
fluid
in
When
b e f o r e t h e OA a e r o s o l .
ileum longitudinal
in a bath w i t h
with
In i n
(Paton and
solution,
was c o l l e c t e d
and
and t h e
measured by b i o l o g i c a l
assay as w e l l as t h e h i s t a m i n e -6 -4 Capsaicin was added a t 10 - t 0 H in t h e bathing
in t h e s t r i p s .
40 min
segments o f
per£used
challenged with antigen
remained
sensitized
before
antigen
challenge,
The bioassay o f histamine was -6 -5 10 - 1 0 H a t t e r desensitization of
in t h e p r e s e n c e o f c a p s a i c i n
ileum t o t h e drug. In a n i m a l s
treated
with
t mg/Kg i . p .
30 min b e f o r e OA a e r o s o l
t i m e was reduced a b o u t 30~ in con~oarison t o c o n t r o l s ,
convulsion
the pre-
whereas
it
remained unchanged when t h e c a p s a i c i n
was adminestered 2 days b e f o r e a e r o s o l
treatment.
effect
A
provocative
drug not
partially
aerosol
protective
was made 3 h a f t e r
the
i n j e c t e d t o t t h r e e c o n s e c u t i v e days. show any p r o t e c t i v e
unsensitized In histamine
in
release.
experiments capsaicin Fig.
last
was
dose (100
shown
pg/Kg
when
i.p.)
of
On t h e o t h e r hand capsaicin
did
e ~ e c t on m i c r o s h o c ~ caused by h i s t a m i n e a e r o s o l
guinea-pigs. vitro
of capsaicin
t
0031-6989/88/20S10017~2/$03.00/0
illustrates
10
-5
that,
-10
-4
M was
found
in t h e s t r i p s
to
in
inhibit
perfused
with
© 1988 The Italian PharmacologicalSociety
18
Pharmacologica/ Research Communications, VoL 20, Supp/ement I, 1988
'°°I
LU < LUZ
..JO IZI..-
50
uJ .
Zz <
I
m -r
ILl 10-6
3 ~10~6
10-s
10. 4
CAPSAICIN ~4 P< o.o2 ¢=~ 1==0,o5
Fig. 1: £ ~ e c ± o~ c a p s a i c i n on the a n a p h y i a c t i c from ileum ]om9i~udinal muscle (n=8), Data are means ! SEH.
histamine release
i_nn vitr__£o,
drug,
h i s t a m i n e r e l e a s e e l i c i t e d by OA was reduced by 35X a t h i g h e r -5 -4 t r a t i o n s (10 - 1 0 H) o f c a p s a i c i n , In
vitro
conclusion
in our p r e l i m i n a r y
and in v i v o a n a p h y l a c t i c
longitudinal
experiments c a p s a i c i n
reaction,
Anaphylactic
affects
histamine
concenthe
in
r e l e a s e #tom
capsaicin
muscle o~ ileum decreased s i g n i f i c a n t l y in the presence o£ -5 -4 t0 - t 0 R. P r e c o n v u l s i o n time is shortened 30 min a f t e r the
injection
o¢ c a p s a i c i n
injection
o£
capsaicin Barnes P.J.
100 pg/Kg
1 mg/kg i.p,
i.p. for
and is
three consecutive
in v i v o appears not to p e r s i s t (tg87].
T,I.P.S.:
Buck S,H. and BurKs T,F, Herxheimer H,
(t952),
8,
(1986}.
J, P h y s i o l ,
Paton W.D,H. and Zar H.A.
(tg68).
lengthened 3 h a ~ t e r days.
The
the e¢£ect
last o~
over time.
24-27. Pharmac. 1t7,
Reviews 38,
t79-226,
25~-255,
J, P h y s i o l ,
tg4+ t 3 - 3 3 ,
S a r i a A. and Lundberg J.H. ( t 9 8 5 ) . In: "ln~larnmatory m e d i a t o r s " , and T , J , W i l l i a m s Eds, VCH P u b l i s h e r , 77-85.
G.A, Higgs
Pharmaco~g~alResearch Commun~ation¢VoL 2~ Supp~mentL 1988 INFLUENCE OF CAPSAICIN ON ANAPHYLACTIC REACTION tN THE GUINEA-PIG S. Franchi
Micheli,
G, Genti l i n i ,
Department o f P r e c l i n i c a l
Key words: h i s t a m i n e ~
due
capsaicin~
is known t h a t
Pharmacology - U n i v e r s i t y
On
the other
including
different
modulatory
role
are
is m o d u l a t e d by a
neurotransmitters. to
be i n v o l v e d
bronchial
of Florence
Since
in
asthma.
effect
and t h i s
o£ n e u r o p e p t i d e s
in neurogenic i n f l a m m a t i o n
t987),
ileum,
neurotransmission
depletion
hand a n a p h y l a x i s
believed
(Barnes~
anaphylaxis,
oapsaicin affects
in p a r t to a r e l e a s e a n d / o r
1986),
they
and C l i n i c a l
S. L u z z i and L. Z i l l e t t i
Morgagni, 65 - 5 0 | 3 4 F l o r e n c e - t e l 055/411133-431364.
V. le G.B,
It
H. C i u f f i ,
(Buck and BurKs,
complex
mechanism
neuropeptides
exert
(S~ria and Lundberg,
pathogenesis
we wanted t o assess t h e e v e n t u a l
of
is
and
1985)
bronchial
interference
a
asthma
of capsaicin
on anaphylaxis.
(0^)
Hale
guinea-pigs
( 3 0 0 - 4 0 0 g) were a c t i v e l y
i,p,
and used f o r
e x p e r i m e n t s 3-4 weeks l a t e r ,
anaphylactic
sensitized
microshocK was p e r f o r m e d by e v a l u a t i n g an-imals exposed t o t h e a n t i g e n a e r o s o l the drug was i n j e c t e d
c a p s a i c i n was used, vitro
experiments,
Zar,
t968)
were
isolated
histamine
fluid
released
performed the
for
i.p,
3 min;
ovalbumin
In in v i v o
experiments
the preconvulsion (Herxheimer,
time
t952)o
Krebs
the perfusion
muscle
Henseleit
fluid
in
When
b e f o r e t h e OA a e r o s o l .
ileum longitudinal
in a bath w i t h
with
In i n
(Paton and
solution,
was c o l l e c t e d
and
and t h e
measured by b i o l o g i c a l
assay as w e l l as t h e h i s t a m i n e -6 -4 Capsaicin was added a t 10 - t 0 H in t h e bathing
in t h e s t r i p s .
40 min
segments o f
per£used
challenged with antigen
remained
sensitized
before
antigen
challenge,
The bioassay o f histamine was -6 -5 10 - 1 0 H a t t e r desensitization of
in t h e p r e s e n c e o f c a p s a i c i n
ileum t o t h e drug. In a n i m a l s
treated
with
t mg/Kg i . p .
30 min b e f o r e OA a e r o s o l
t i m e was reduced a b o u t 30~ in con~oarison t o c o n t r o l s ,
convulsion
the pre-
whereas
it
remained unchanged when t h e c a p s a i c i n
was adminestered 2 days b e f o r e a e r o s o l
treatment.
effect
A
provocative
drug not
partially
aerosol
protective
was made 3 h a f t e r
the
i n j e c t e d t o t t h r e e c o n s e c u t i v e days. show any p r o t e c t i v e
unsensitized In histamine
in
release.
experiments capsaicin Fig.
last
was
dose (100
shown
pg/Kg
when
i.p.)
of
On t h e o t h e r hand capsaicin
did
e ~ e c t on m i c r o s h o c ~ caused by h i s t a m i n e a e r o s o l
guinea-pigs. vitro
of capsaicin
t
0031-6989/88/20S10017~2/$03.00/0
illustrates
10
-5
that,
-10
-4
M was
found
in t h e s t r i p s
to
in
inhibit
perfused
with
© 1988 The Italian PharmacologicalSociety
18
Pharmacologica/ Research Communications, VoL 20, Supp/ement I, 1988
'°°I
LU < LUZ
..JO IZI..-
50
uJ .
Zz <
I
m -r
ILl 10-6
3 ~10~6
10-s
10. 4
CAPSAICIN ~4 P< o.o2 ¢=~ 1==0,o5
Fig. 1: £ ~ e c ± o~ c a p s a i c i n on the a n a p h y i a c t i c from ileum ]om9i~udinal muscle (n=8), Data are means ! SEH.
histamine release
i_nn vitr__£o,
drug,
h i s t a m i n e r e l e a s e e l i c i t e d by OA was reduced by 35X a t h i g h e r -5 -4 t r a t i o n s (10 - 1 0 H) o f c a p s a i c i n , In
vitro
conclusion
in our p r e l i m i n a r y
and in v i v o a n a p h y l a c t i c
longitudinal
experiments c a p s a i c i n
reaction,
Anaphylactic
affects
histamine
concenthe
in
r e l e a s e #tom
capsaicin
muscle o~ ileum decreased s i g n i f i c a n t l y in the presence o£ -5 -4 t0 - t 0 R. P r e c o n v u l s i o n time is shortened 30 min a f t e r the
injection
o¢ c a p s a i c i n
injection
o£
capsaicin Barnes P.J.
100 pg/Kg
1 mg/kg i.p,
i.p. for
and is
three consecutive
in v i v o appears not to p e r s i s t (tg87].
T,I.P.S.:
Buck S,H. and BurKs T,F, Herxheimer H,
(t952),
8,
(1986}.
J, P h y s i o l ,
Paton W.D,H. and Zar H.A.
(tg68).
lengthened 3 h a ~ t e r days.
The
the e¢£ect
last o~
over time.
24-27. Pharmac. 1t7,
Reviews 38,
t79-226,
25~-255,
J, P h y s i o l ,
tg4+ t 3 - 3 3 ,
S a r i a A. and Lundberg J.H. ( t 9 8 5 ) . In: "ln~larnmatory m e d i a t o r s " , and T , J , W i l l i a m s Eds, VCH P u b l i s h e r , 77-85.
G.A, Higgs