Insulin and glucagon secretion in isolated pancreatic islets from rats with CCL4-induced cirrhosis

First Dcps?tment of Anatomy and dicine, ~emmelweis University Medical Somatostetin and substance P imwnoreaotive nerve slcmcntcr of the liver were studied with immun~cytw3hemicaL teobniques st light- and electronmicLOBOOP~D level. Some irnmunoresctive nf~rve periksrya could be observed in a 8mslL ganglia. There were nUmcl~“s tmmuhoreactive ne~“e fibres around the artezies end arterioles and some arwnd the veins of the interlobular vessels. A fen immunoreactive nervefibres were-also found in close asso~iati~n wLth thB Central vein and liver sinuses. The immunoreactive nerve prooeew~ were varicose in appearance ar.2 often ran singly or in grwpe of 2-3. l’hey contained clear vesicles of 40 to 60 nm and large granulated vesicles oP 80 to 120 nm in diameter. In some cases they were close to the smoHh qua.,le cells of the blood vessels. ‘The gap between the labelled nerve Pibres and tbe smooth muscle cell memb~ansiv88 20 to 2000 nm. In some cases swnstostetin immunoreactive “er”s cell bodies owld be Observed in the intralobular 0alxlective tLs8Ue an.3 in 8 “try 01ose vincinity to the sinus endcxthelial cells. In several cases some of the endathelial cells of the ainua showed alSO the BonatDetatin imnunoleactivity. The number of the aomatoststin i!ununoreactive nex~e fibres ~88 larger then the substance P fibers. It is concluded that these neuropeptide containing nerve fibres ma” iRfluence the blood flow 8nd mar zegulate the fu”ctiDn of the liver

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INSULIN AND GLUCAGON SECRETION IN ISOLATED PANCREATIC ISLETS FROM KATS WlTH CCLIINDUCED CIRRHOSIS J Fernjinder-qlv&rez. P Pizcoeta. R Goais. C l&s. R Casnmrtpnn. : Sosch. J Radis Dlabeten "mt. L,ver "nit. Hospital Clinic

1Pro”,nclal. “mvelntyof Barcelona.

Pat~e”t~ w,th c,rrhos,s of thelwerfrequently

exhib,t ,"creased ,"su,i" and glucago" plasma ,eve1*. Hwwer the mectlamrm le..ang to h~penasul~nlsm and hiperglucagomsm rema,ns u"c,enr. The a,m of the present study was ~"vest~gate the ,"sul," and glucago" secretion in Isolated pancreatic ~slels obtalned from rats with experrmental cirrhrsrs and parred controls. Cxrrhos~r w&s rnduced by coab~ned CCWpheoobarbxtal adm,",stratlon for 10 *eels. Pancreatic lsletr were Isolated by the collagenase method and incubated for 90 m,n. at 37'C. I" blcarbanate-buffered mcdrum contaimng d,ferent co"ce"trat,o" of glucose and argimne. The release of giucago" (IRG) (pglislet 90 mm.) and ~"sul~n (IRl) (uU/lslet 90 min., was measured by RI!!.ReQIltr ape rummansed I" the table (Mean+SrJ): Glucose 3.3 mM Glucose 3.3ti + Arqrmne i0.M Glucose 16.4 ~$4 IR1 IRG IRI IRG 1RG 111 Control 26~10 154+109 45118 286+248 198_t33 124+97 n=14 n=19 n=14 "~20 n-14 n=26

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