- Email: [email protected]
Isolation and characterization of a lipopeptide biosurfactant from a Pseuomonas sp. associated with the marine sponge Phakeliia sp.
Abstracts / Journal of Biotechnology 136S (2008) S589–S601
VI6-P-024 Characteristics of denaturized chondroitin sulfate from sturgeon Jiang Zheng ∗ , Yonghua Tian, Ruizhang Guan Key Laboratory of Science and Technology for Aquaculture and Food Safety in Fujian Province University, Fisheries College of Jimei University, Xiamen 361021, China E-mail addresses: [email protected] (J. Zheng), [email protected] (R. Guan). Chondroitin sulfate (CS) extracted from elasmobranch has been widely used in medicine, food and cosmetic industries (Mourano et al., 1996; Timer et al., 1995; Ronca et al., 1998). However, the quality of CS is often affected by its denaturation. To assess and analyze the quality of CS product from sturgeon, the characteristics of the denaturized CS were comparatively studied. Compared with the normal CS from sturgeon, the denaturized simple shows a significant decrease in the molecular weight, the power rotation, and the contents of glucuronic acid and sulfate group. In UV and visible spectrum, the denaturized CS has a much more higher absorption than the normal CS between wavelength 240 and 800 nm. In the Fourier-transform infrared spectra, denaturized CS has a special vibration at the wavenumber 1040 cm−1 ; the ratios of transmittance in 1068 cm−1 to that in 1642 cm−1 (T1068 /T1642 ) are remarkable different between the denaturized CS and the normal. The T1068 /T1642 is 1.12 for the denaturized CS, while 0.85 for the normal CS. These results provide the basic data for the assessment and analysis of CHS product quality. They also help us have a deep understanding of the nature of CS denaturation. Acknowledgement The research was supported by Project of Science and Technology of Xiamen City (No. 3502Z20031048). References Mourano, P.A.S., Pereira, M.S., Pavao, M.S.G., et al., 1996. Structure and anticoagulant activity of fucosylated condroitin sulfate from echinodem sulfated fucose branches on the polysaccharides account for its high anticoagulant action. J. Biol. Chem. 271 (39), 23973–23979. Ronca, F., Palmieri, L., Panicucci, P., et al., 1998. Anti-inflammatory activity of chondroitin sulfate. Osteoarthritis Cartilage 6, 14. Timer, J., Diczhazi, C., Bartha, I., et al., 1995. Modulation of heparan sulfate/chondroitin sulfate ratio by glycosaminoglycan biosynthesis inhibitors affects liver metabotic potential of tumor cells. Int. J. Cancer 62 (6), 755–769.
doi:10.1016/j.jbiotec.2008.07.1207 VI6-P-025 Isolation and characterization of a lipopeptide biosurfactant from a Pseuomonas sp. associated with the marine sponge Phakeliia sp. Hong-man Hou 1,2,∗ , Li-li Zhai 1 , Peng Xu 1 , Xing-ju Yu 2 , Wei Zhang 2 1 College of Bio & Food Technology, Dalian Polytechnic University, Dalian 116034, China 2 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
E-mail address: [email protected] (H.-m. Hou). Lipopeptide is a kind of biosurfactant synthesized by microorganisms. In addition to the classical application as surfactant,
S597
some unique bioactive functions were also being focused. Marine sponges are well-known as conglomerates of various organisms. Good oil emulsification effect was found during the enrichment culture of the sponge Phakeliia sp. extracts. In this study, bacteria producing biosurfactant were isolated from the sponge by oil spreading technique, and the lipopeptide biosurfactant was extracted and characterized on the surface activity, physicochemical property and bioactivities (Noha et al., 2004). 62 strains isolated from the sponge extracts were proved that three of them had the abilities to produce biosurfactant. The three isolated strain were designated as XP1, XP2, H10. 16S ribosomal DNA sequencing revealed that strains XP1, XP2, H10 belonged to the genus Pseudomonas, Bacillus pumilus and Bacillus subtilis, respectively. Strain XP1 was selected for its best surface activity. Its biosurfactant was identified as a type of lipopeptide and purified by Sephadex LH-20 and ODS column, named as XP-BS. The XP-BS lowered the surface tension of water to 26.5 dyn/cm, and the CMC was 40 M. The XP-BS was stable at pH 2.0–8.5, which was different from other biosurfactants (Christian et al., 2003; Stanislav et al., 2003). Moreover, it showed great thermal stability at 100 ◦ C and 121 after incubation for 2 h and 20 min, respectively. The function of XP-BS was not affected at a salt concentration of 1000 mM for NaCl and 10 mM for CaCl2 . XP-BS was tested against a set of standard Gram-positive, Gram-negative, and eukaryotic microorganisms. It exhibited a considerable antimicrobial activity against Staphylococcus aureus and Escherichia coli, a lower activity against epiphyte. XP-BS also showed certain superiority to other chemically synthesized surfactants. All the above results suggested that the applications of XP-BS could be developed commercially. References Christian, T.P., Joachim, V., Christopher, W., 2003. Antimicrobial activities and matrixassisted laser desorption/ionization mass spectrometry of bacillus isolates from the marine sponge Aplysina aerophoba. Mar. Biotechnol. 5, 424–434. Noha, H.Y., Kathleen, E.D., David, P.N., 2004. Comparison of methods to detect biosurfactant production by diverse microorganisms. J. Microbiol. Methods 56, 339–347. Stanislav, G.B., Tatiana, A.R., Stanislav, E.E., 2003. A novel lipopeptide, an inhibitor of bacterial adhesion from the thermophilic and halotolerant subsurface Bacillus licheniformis strain 603. Biochim. Biophys. Acta 1634, 107–115.
doi:10.1016/j.jbiotec.2008.07.1208 VI6-P-026 Study on preparation and stability of phycobiliprotein microcapsulates Jiang Zheng Key Laboratory of Science and Technology for Aquaculture and Food Safety in Fujian Province University, Fisheries College of Jimei University, Xiamen 361021, China E-mail address: [email protected]. Phycobiliprotein is a kind of bioactive materials that has been widely used in food and medical fields (Zhang et al., 1999a). However, the active and unstable characters of phycobiliprotein limit its use (Zhang et al., 1999b). In the present paper, phycobiliprotein was encapsulated by the method of chitosan-sodiumaiginate, then the preparation condition and stability of the phycobiliprotein microcapsules were studied and discussed. The results show that the proper condition for the preparation of the microcapsules is 3% of sodium alginate, 3% CaCl2 and 0.5% chitosan. After encapsulated, the endurable time of phycobiliprotein in microcapsulates to heat is 5–10 times as long as that in water solution. The stabilities
VI6-P-024 Characteristics of denaturized chondroitin sulfate from sturgeon Jiang Zheng ∗ , Yonghua Tian, Ruizhang Guan Key Laboratory of Science and Technology for Aquaculture and Food Safety in Fujian Province University, Fisheries College of Jimei University, Xiamen 361021, China E-mail addresses: [email protected] (J. Zheng), [email protected] (R. Guan). Chondroitin sulfate (CS) extracted from elasmobranch has been widely used in medicine, food and cosmetic industries (Mourano et al., 1996; Timer et al., 1995; Ronca et al., 1998). However, the quality of CS is often affected by its denaturation. To assess and analyze the quality of CS product from sturgeon, the characteristics of the denaturized CS were comparatively studied. Compared with the normal CS from sturgeon, the denaturized simple shows a significant decrease in the molecular weight, the power rotation, and the contents of glucuronic acid and sulfate group. In UV and visible spectrum, the denaturized CS has a much more higher absorption than the normal CS between wavelength 240 and 800 nm. In the Fourier-transform infrared spectra, denaturized CS has a special vibration at the wavenumber 1040 cm−1 ; the ratios of transmittance in 1068 cm−1 to that in 1642 cm−1 (T1068 /T1642 ) are remarkable different between the denaturized CS and the normal. The T1068 /T1642 is 1.12 for the denaturized CS, while 0.85 for the normal CS. These results provide the basic data for the assessment and analysis of CHS product quality. They also help us have a deep understanding of the nature of CS denaturation. Acknowledgement The research was supported by Project of Science and Technology of Xiamen City (No. 3502Z20031048). References Mourano, P.A.S., Pereira, M.S., Pavao, M.S.G., et al., 1996. Structure and anticoagulant activity of fucosylated condroitin sulfate from echinodem sulfated fucose branches on the polysaccharides account for its high anticoagulant action. J. Biol. Chem. 271 (39), 23973–23979. Ronca, F., Palmieri, L., Panicucci, P., et al., 1998. Anti-inflammatory activity of chondroitin sulfate. Osteoarthritis Cartilage 6, 14. Timer, J., Diczhazi, C., Bartha, I., et al., 1995. Modulation of heparan sulfate/chondroitin sulfate ratio by glycosaminoglycan biosynthesis inhibitors affects liver metabotic potential of tumor cells. Int. J. Cancer 62 (6), 755–769.
doi:10.1016/j.jbiotec.2008.07.1207 VI6-P-025 Isolation and characterization of a lipopeptide biosurfactant from a Pseuomonas sp. associated with the marine sponge Phakeliia sp. Hong-man Hou 1,2,∗ , Li-li Zhai 1 , Peng Xu 1 , Xing-ju Yu 2 , Wei Zhang 2 1 College of Bio & Food Technology, Dalian Polytechnic University, Dalian 116034, China 2 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
E-mail address: [email protected] (H.-m. Hou). Lipopeptide is a kind of biosurfactant synthesized by microorganisms. In addition to the classical application as surfactant,
S597
some unique bioactive functions were also being focused. Marine sponges are well-known as conglomerates of various organisms. Good oil emulsification effect was found during the enrichment culture of the sponge Phakeliia sp. extracts. In this study, bacteria producing biosurfactant were isolated from the sponge by oil spreading technique, and the lipopeptide biosurfactant was extracted and characterized on the surface activity, physicochemical property and bioactivities (Noha et al., 2004). 62 strains isolated from the sponge extracts were proved that three of them had the abilities to produce biosurfactant. The three isolated strain were designated as XP1, XP2, H10. 16S ribosomal DNA sequencing revealed that strains XP1, XP2, H10 belonged to the genus Pseudomonas, Bacillus pumilus and Bacillus subtilis, respectively. Strain XP1 was selected for its best surface activity. Its biosurfactant was identified as a type of lipopeptide and purified by Sephadex LH-20 and ODS column, named as XP-BS. The XP-BS lowered the surface tension of water to 26.5 dyn/cm, and the CMC was 40 M. The XP-BS was stable at pH 2.0–8.5, which was different from other biosurfactants (Christian et al., 2003; Stanislav et al., 2003). Moreover, it showed great thermal stability at 100 ◦ C and 121 after incubation for 2 h and 20 min, respectively. The function of XP-BS was not affected at a salt concentration of 1000 mM for NaCl and 10 mM for CaCl2 . XP-BS was tested against a set of standard Gram-positive, Gram-negative, and eukaryotic microorganisms. It exhibited a considerable antimicrobial activity against Staphylococcus aureus and Escherichia coli, a lower activity against epiphyte. XP-BS also showed certain superiority to other chemically synthesized surfactants. All the above results suggested that the applications of XP-BS could be developed commercially. References Christian, T.P., Joachim, V., Christopher, W., 2003. Antimicrobial activities and matrixassisted laser desorption/ionization mass spectrometry of bacillus isolates from the marine sponge Aplysina aerophoba. Mar. Biotechnol. 5, 424–434. Noha, H.Y., Kathleen, E.D., David, P.N., 2004. Comparison of methods to detect biosurfactant production by diverse microorganisms. J. Microbiol. Methods 56, 339–347. Stanislav, G.B., Tatiana, A.R., Stanislav, E.E., 2003. A novel lipopeptide, an inhibitor of bacterial adhesion from the thermophilic and halotolerant subsurface Bacillus licheniformis strain 603. Biochim. Biophys. Acta 1634, 107–115.
doi:10.1016/j.jbiotec.2008.07.1208 VI6-P-026 Study on preparation and stability of phycobiliprotein microcapsulates Jiang Zheng Key Laboratory of Science and Technology for Aquaculture and Food Safety in Fujian Province University, Fisheries College of Jimei University, Xiamen 361021, China E-mail address: [email protected]. Phycobiliprotein is a kind of bioactive materials that has been widely used in food and medical fields (Zhang et al., 1999a). However, the active and unstable characters of phycobiliprotein limit its use (Zhang et al., 1999b). In the present paper, phycobiliprotein was encapsulated by the method of chitosan-sodiumaiginate, then the preparation condition and stability of the phycobiliprotein microcapsules were studied and discussed. The results show that the proper condition for the preparation of the microcapsules is 3% of sodium alginate, 3% CaCl2 and 0.5% chitosan. After encapsulated, the endurable time of phycobiliprotein in microcapsulates to heat is 5–10 times as long as that in water solution. The stabilities