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Isolation of secoisolariciresinol diglucoside from defatted flaxseed by AB-8 macroporous resin
S498
Abstracts / Journal of Biotechnology 136S (2008) S496–S505
V6-P-002 Isolation of secoisolariciresinol diglucoside from defatted flaxseed by AB-8 macroporous resin Chunzhi Zhang ∗ , Yi Liu, Guoren Zu, Tao Wan, Wenzhong Wu College of Bio. & Food Technology, Dalian Polytechnic University, Dalian 116034, China E-mail address: [email protected] (C. Zhang). Secoisolariciresinol diglucoside (SDG) is a phytoestrogen, which balances the hormone level in vivo and plays a preventive role in breast cancer, prostate cancer, bone loosen and type 2 diabetes. It reduces the serum cholesterol and hypercholesterolemic atherosclerosis, and also has the antioxidant activity (Lemay et al., 2002). It is highly effective to isolate SDG by high-performance liquid chromatography or high-speed counter-current chromatography (Degenhardt et al., 2002), but they are not fit for SDG production in a large scale because of the special equipments or high cost. Yeast was used for enhancing extraction of SDG by disintegrating flaxseed cells and mixed enzymes were used for getting rid of polysaccharides and proteins by hydrolysis, which contributed to the further separation of SDG. Alkaline hydrolysis was essential to liberate SDG because the natural SDG existed as an oligomer. It was hardly to obtain SDG without alkaline hydrolysis, so sodium hydroxide was employed to do that (Eliasson et al., 2003). AB-8 macroporous resin was employed for the further separation. Crude SDG was applied to the AB-8 column. After rinsing with 8 l of water, elution of an SDG-enriched fraction was carried out with 6 l of 30% of ethanol–water. The eluate was concentrated by vacuum distillation and evaporated to dryness in vacuo drier to yield 22.5 g of SDG starting from 1 kg of defatted flaxseed. It was a simple and low-cost process, in which the purity of isolated SDG was 55%. It was suitable to produce SDG in a large scale. Acknowledgement
static foams. Experiments were conducted to investigate the effect of bulk concentration on the rate and extent of adsorption (Feitosa et al., 2006; Kruglyakov and Khaskova, 2005). It was found that the adsorption rate increased with the bulk concentration of nisin and the relationship between equilibrium surface concentration and bulk concentration followed a Langmiur fashion. Surface concentrations of nisin at constant bulk concentration and different liquid pool depths were also measured to investigate the rate of surface accumulation. Experimental data were fitted into a modified Logistical equation and a phenomenological model was developed to empirically simulate the adsorption process. Thus surface concentration can be expected to increase with increase in both bulk concentration and residence time. References Saleh, Z.S., Hossain, M.M., 2001. A study of the separation of proteins from multicomponent mixtures by a semi-batch foaming process. Chem. Eng. Process. 40, 371–378. Feitosa, K., Halt, O.L., Kamien, R.D., Durian, D.J., 2006. Bubble kinetics in a steady-state column of aqueous foam. Europhys. Lett. 76, 683–689. Kruglyakov, P.M., Khaskova, T.N., 2005. Adsorption accumulation of proteins and dyes in foams of solutions and waste water. Colloids Surf. A: Physicochem. Eng. Aspects 263, 400–404, Supported by Nature Sciense Foundation of Tianjin (08JCZDJC25200).
doi:10.1016/j.jbiotec.2008.07.1167 V6-P-004 Study on removal of trace FE3+ from aqueous solution by foam fractionation Huijie Zheng ∗ , Yingjun Jing, Zhaoliang Wu Department of Bioengineering, Hebei University of Technology, Tianjin, PR China E-mail address: [email protected] (H. Zheng).
doi:10.1016/j.jbiotec.2008.07.1166
Removing Fe3+ from simulated aqueous solution by foam fractionation was studied using C18 H30 SO3 (DBAS) as the carrier surfactant (Chen et al., 1994; Yenidunya, 2006), especially effects of gas flowrate, pH, surfactant dosage and load volume were examined (Du et al., 2003; Poxon and Hughes, 1999), the four variables were determined by response surface methodology (RSM) based on the central composite design (CCD) (Bustos et al., 2004). The experimental results showed that DBAS had good foam performance and Fe3+ could be effectively removed from aqueous solutions by foam fractionation, the optimum experimental condition was the gas flow rate 0.05 m3 /h, pH 4.0, surfactant concentration 0.16 g/L, load volume 1.0 L, Enrichment ratio reached 9.86, and removal rate 98.63%. Comparing with the commonly used surfactant—sodium dodecyl sulfate, DBAS would not leave Na+ in the aqueous solutions (Morgan and Wiesmann, 2002), thus a new idea was put forward for removing ions or salts by foam fractionation.
V6-P-003
References
This work was supported by The Educational Department of Liaoning Province, China. References Degenhardt, A., Habben, S., Winterhalter, P., 2002. Isolation of the lignan secoisolariciresinol diglucoside from flaxseed (Linum usitatissimum L.) by high-speed counter-current chromatography. J. Chromatogr. A 943, 299–302. Eliasson, C., Kamal-Eldin, A., Andersson, R., Aman, P., 2003. High-performance liquid chromatographic analysis of secoisolariciresinol diglucoside and hydroxycinnamic acid glucosides in flaxseed by alkaline extraction. J. Chromatogr. A 1012, 151–159. Lemay, A., Dodin, S., Kadri, N., Jacques, H., Forest, J.C., 2002. Flaxseed dietary supplement versus hormone replacement therapy in hypercholesterolemic menopausal women. Obstet. Gynecol. 100, 495–504.
Adsorption of nisin onto bubble surfaces in a batch foam fractionator: Kinetics study Zhaoliang Wu ∗ , Huijie Zheng, Xueliang Li Department of Biochemical Engineering, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, PR China E-mail address: [email protected] (Z. Wu). The adsorption behavior of nisin onto bubble surfaces in a batch foam fractionator (Saleh and Hossain, 2001) was studied using
Chen, S., Timmons, M.B., Bisogni, J.J., Aneshansley, D.J., 1994. Modeling surfactant removal in foam fractionation. I. Theoretical development. Aquacult. Eng. 13, 163–181. Yenidunya, M.D., 2006. Recovery of Zn(II), Mn(II) and Cu(II) in aqueous solutions by foam fractionation with sodium dodecyl sulphate in combination with chelating agents. Sep. Sci. Technol. 41, 1741–1756. Du, L.P., Prokop, A., Tanner, R.D., 2003. Effect of pH on the startup of a continuous foam fractionation process containing ovalbumin. Sep. Sci. Technol. 38, 1093–1109. Poxon, S.W., Hughes, J.A., 1999. Foam fractionation as a method to separate endotoxin from recombinant biotechnology products. J. Pharm. Sci. Technol. 53, 23–26.
Abstracts / Journal of Biotechnology 136S (2008) S496–S505
V6-P-002 Isolation of secoisolariciresinol diglucoside from defatted flaxseed by AB-8 macroporous resin Chunzhi Zhang ∗ , Yi Liu, Guoren Zu, Tao Wan, Wenzhong Wu College of Bio. & Food Technology, Dalian Polytechnic University, Dalian 116034, China E-mail address: [email protected] (C. Zhang). Secoisolariciresinol diglucoside (SDG) is a phytoestrogen, which balances the hormone level in vivo and plays a preventive role in breast cancer, prostate cancer, bone loosen and type 2 diabetes. It reduces the serum cholesterol and hypercholesterolemic atherosclerosis, and also has the antioxidant activity (Lemay et al., 2002). It is highly effective to isolate SDG by high-performance liquid chromatography or high-speed counter-current chromatography (Degenhardt et al., 2002), but they are not fit for SDG production in a large scale because of the special equipments or high cost. Yeast was used for enhancing extraction of SDG by disintegrating flaxseed cells and mixed enzymes were used for getting rid of polysaccharides and proteins by hydrolysis, which contributed to the further separation of SDG. Alkaline hydrolysis was essential to liberate SDG because the natural SDG existed as an oligomer. It was hardly to obtain SDG without alkaline hydrolysis, so sodium hydroxide was employed to do that (Eliasson et al., 2003). AB-8 macroporous resin was employed for the further separation. Crude SDG was applied to the AB-8 column. After rinsing with 8 l of water, elution of an SDG-enriched fraction was carried out with 6 l of 30% of ethanol–water. The eluate was concentrated by vacuum distillation and evaporated to dryness in vacuo drier to yield 22.5 g of SDG starting from 1 kg of defatted flaxseed. It was a simple and low-cost process, in which the purity of isolated SDG was 55%. It was suitable to produce SDG in a large scale. Acknowledgement
static foams. Experiments were conducted to investigate the effect of bulk concentration on the rate and extent of adsorption (Feitosa et al., 2006; Kruglyakov and Khaskova, 2005). It was found that the adsorption rate increased with the bulk concentration of nisin and the relationship between equilibrium surface concentration and bulk concentration followed a Langmiur fashion. Surface concentrations of nisin at constant bulk concentration and different liquid pool depths were also measured to investigate the rate of surface accumulation. Experimental data were fitted into a modified Logistical equation and a phenomenological model was developed to empirically simulate the adsorption process. Thus surface concentration can be expected to increase with increase in both bulk concentration and residence time. References Saleh, Z.S., Hossain, M.M., 2001. A study of the separation of proteins from multicomponent mixtures by a semi-batch foaming process. Chem. Eng. Process. 40, 371–378. Feitosa, K., Halt, O.L., Kamien, R.D., Durian, D.J., 2006. Bubble kinetics in a steady-state column of aqueous foam. Europhys. Lett. 76, 683–689. Kruglyakov, P.M., Khaskova, T.N., 2005. Adsorption accumulation of proteins and dyes in foams of solutions and waste water. Colloids Surf. A: Physicochem. Eng. Aspects 263, 400–404, Supported by Nature Sciense Foundation of Tianjin (08JCZDJC25200).
doi:10.1016/j.jbiotec.2008.07.1167 V6-P-004 Study on removal of trace FE3+ from aqueous solution by foam fractionation Huijie Zheng ∗ , Yingjun Jing, Zhaoliang Wu Department of Bioengineering, Hebei University of Technology, Tianjin, PR China E-mail address: [email protected] (H. Zheng).
doi:10.1016/j.jbiotec.2008.07.1166
Removing Fe3+ from simulated aqueous solution by foam fractionation was studied using C18 H30 SO3 (DBAS) as the carrier surfactant (Chen et al., 1994; Yenidunya, 2006), especially effects of gas flowrate, pH, surfactant dosage and load volume were examined (Du et al., 2003; Poxon and Hughes, 1999), the four variables were determined by response surface methodology (RSM) based on the central composite design (CCD) (Bustos et al., 2004). The experimental results showed that DBAS had good foam performance and Fe3+ could be effectively removed from aqueous solutions by foam fractionation, the optimum experimental condition was the gas flow rate 0.05 m3 /h, pH 4.0, surfactant concentration 0.16 g/L, load volume 1.0 L, Enrichment ratio reached 9.86, and removal rate 98.63%. Comparing with the commonly used surfactant—sodium dodecyl sulfate, DBAS would not leave Na+ in the aqueous solutions (Morgan and Wiesmann, 2002), thus a new idea was put forward for removing ions or salts by foam fractionation.
V6-P-003
References
This work was supported by The Educational Department of Liaoning Province, China. References Degenhardt, A., Habben, S., Winterhalter, P., 2002. Isolation of the lignan secoisolariciresinol diglucoside from flaxseed (Linum usitatissimum L.) by high-speed counter-current chromatography. J. Chromatogr. A 943, 299–302. Eliasson, C., Kamal-Eldin, A., Andersson, R., Aman, P., 2003. High-performance liquid chromatographic analysis of secoisolariciresinol diglucoside and hydroxycinnamic acid glucosides in flaxseed by alkaline extraction. J. Chromatogr. A 1012, 151–159. Lemay, A., Dodin, S., Kadri, N., Jacques, H., Forest, J.C., 2002. Flaxseed dietary supplement versus hormone replacement therapy in hypercholesterolemic menopausal women. Obstet. Gynecol. 100, 495–504.
Adsorption of nisin onto bubble surfaces in a batch foam fractionator: Kinetics study Zhaoliang Wu ∗ , Huijie Zheng, Xueliang Li Department of Biochemical Engineering, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, PR China E-mail address: [email protected] (Z. Wu). The adsorption behavior of nisin onto bubble surfaces in a batch foam fractionator (Saleh and Hossain, 2001) was studied using
Chen, S., Timmons, M.B., Bisogni, J.J., Aneshansley, D.J., 1994. Modeling surfactant removal in foam fractionation. I. Theoretical development. Aquacult. Eng. 13, 163–181. Yenidunya, M.D., 2006. Recovery of Zn(II), Mn(II) and Cu(II) in aqueous solutions by foam fractionation with sodium dodecyl sulphate in combination with chelating agents. Sep. Sci. Technol. 41, 1741–1756. Du, L.P., Prokop, A., Tanner, R.D., 2003. Effect of pH on the startup of a continuous foam fractionation process containing ovalbumin. Sep. Sci. Technol. 38, 1093–1109. Poxon, S.W., Hughes, J.A., 1999. Foam fractionation as a method to separate endotoxin from recombinant biotechnology products. J. Pharm. Sci. Technol. 53, 23–26.