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It Is Worth Step Sectioning in Minor Salivary Gland Evaluation for Sjögren's Syndrome
OOOO Volume 119, Number 3 IATROGENIC ANGIOMATOUS KAPOSI’S SARCOMA OF THE GINGIVA IN A PATIENT WITH SMALL LYMPHOCYTIC LYMPHOMA/CHRONIC LYMPHOCYTIC LEUKEMIA Sulen Sarioglu1, Sermin Ozkal1, Güray Akturk1, Ersoy Dogan2, Mehmet Arici2, Fatih Demirkan3, 1Dokuz Eylul University, Faculty of Medicine, Department of Pathology, Izmir, Turkey; 2Dokuz Eylul University, Faculty of Medicine, Department of Otorhinolaryngology and Head and Neck Surgery, Izmir, Turkey; 3Dokuz Eylul University, Faculty of Medicine, Department of Hematology, Izmir, Turkey Fifty year old male patient presented with gingival hypertrophic, purple lesion of 4 cm diameter. He had a history of small lymphocytic lymphoma/chronic lymphocytic leukemia with deletion of 13q14.3; diagnosed 9 months ago. He had received fularabine, retoximabe and cyclosporine theraphy was induced and 6 cures could be admitted. Due to low platelet counts biopsy could be performed at the 3rd month. The biopsy revealed surface mucosal ulceration and a submucosal mass of spindle cells and vascular structures filled with blood cells. There was cellular atypia and few atypical mitosis. HHV8, CD31, CD38, D2-40 were positive and ki-67 index was 29%. Final diagnosis was angiomatous type Kaposi sarcoma (KS). The patient was HIV negative. KS of the oral cavity is most frequent in HIV positive patients but, however exceedingly rara, this diagnosis should be kept in mind in patients in cases receiving chemoteraphy for lymphoma. Keywords: Kaposi’s sarcoma, iatrogenic, gingiva, lypmhoma
CHROMOSOMAL INSTABILITY IN ORAL POTENTIALLY MALIGNANT DISORDERS Zuraiza Zaini, Edward Odell, Mahvash Tavassoli, Clinical and Diagnostic Sciences, Dental Institute, King’s College London, UK Objective: To compare potential routine diagnostic tests for chromosomal instability (CI) in oral epithelial dysplasia. Study Design: A gene signature based on copy number changes detected by SNP-chip analysis was tested on 20 oral dysplastic lesions with known ploidy status (7 diploid, 13 aneuploid) by QuantiGeneÒ Plex DNA Assay (Affymetrix) and qPCR (Applied Biosystems) using matched normal tissue and cell lines as control. FISH was performed to evaluate CI in chromosomes 3, 7 and 8 using a subset of targets. Results: Neither QuantiGeneÒPlex or qPCR accurately measured CI, being dependent on housekeeping gene used for normalisation. Only FISH proved sensitive, detecting 4 out of 12 cases as aneuploid using a combination of 2 targets. Conclusions: High throughput copy number assessments were unable to assess CI in oral dysplasia. FISH successfully detected CI, but at lower sensitivity than image-based DNA ploidy analysis. Keywords: Oral potentially malignant disorders, Chromosomal instability, Ploidy analysis, FISH, QuantiGene Plex, realtime PCR
IT IS WORTH STEP SECTIONING IN MINOR SALIVARY ¨ GREN’S SYNDROME GLAND EVALUATION FOR SJO Sulen Sarioglu1, Ulku Kucuk3, Pınar Çetin2, Ismail Sarı2, Merih Birlik2, 1Dokuz Eylul University, Faculty of Medicine, Department of Pathology, Izmir, Turkey; 2Dokuz Eylul University, Faculty of Medicine, Department of Rheumatology, Izmir, Turkey; 3Izmir Tepecik Research and Training Hospital, Izmir, Turkey
ABSTRACTS Abstracts e195 Objective: In this series the value of step sectioning and plasma cell focus are revisited in a series of salivary gland biopsies. Study Design: Three step sections at 200micronmeter in between, were reeavaluated seperately, for both lymphocyte and plasma cell foci, from biopsies of fourty-three cases with a diagnosis of scleroderma and suspected of Sjögren’s Syndrome (SS). Results: Lymphocytic focus was identified in 28(60,2%) cases. If the highest focus score among the three sections was considered, focus score was >¼1 in 24(85,71%) cases. Considering both focus scores are diagnostic for SS, the sections with the lowest scores would change the diagnostic category in 11(39,3%) of the cases and the lowest focus score was 0 in 11 cases (39,3%). Plasma cell focus was observed in 6(13,9%) cases, however all had a focus score higher than 1. Conclusions: These results highlight the importance of step sectioning of the minor salivary gland biopsies. Keywords: Sjögren’s Syndrome, step sectioning, minor salivary gland biopsy, plasma cell foci.
RETROSPECTIVE STUDY OF TRIGEMINAL NEURALGIA AT FACULTY OF DENTISTRY, CHULALONGKORN UNIVERSITY Kanokporn Bhalang, Department of Oral Medicine, Chulalongkorn University, Bangkok, Thailand Objective: The development of pain in orofacial locations stems from a variety of disorders. The most common neuropathic pain condition in the orofacial area is trigeminal neuralgia. Study Design: This study retrospectively examined 149 trigeminal neuralgia patients at the Department of Oral Medicine, Faculty of Dentistry, Chulalongkorn University. Results: The patients’ age range was 20-85 years old. The ratio of females to males was two to one. The most common trigger zone was located at the lower right posterior teeth. One third of the patients had dental treatments performed in the area of chief complaint prior to their visits to the school. Carbamazepine was mostly used for this disorder. The lowest dose that effectively controlled the pain was 200 mg per day. The side effects of the drug were vertigo, nausea, vomiting and decreased white blood cell counts. Conclusions: Carbamazepine was the drug of choice for trigeminal neuralgia. Keywords: Trigeminal neuralgia, carbamazepine, retrospective
TUMOR BUDDING IN ORAL CAVITY CARCINOMAS Sulen Sarioglu1, Oguz Cetinayak2, Barbaros Aydın2, Ersoy Dogan3, Fadime Akman2, Ahmet Omer Ikiz3, 1Dokuz Eylul University, Faculty of Medicine, Department of Pathology, Izmir, Turkey; 2Dokuz Eylul University, Faculty of Medicine, Department of Radiation Oncology, Izmir, Turkey; 3 Dokuz Eylul University, Faculty of Medicine, Department of Otorhinolaryngology Head and Neck Surgery, Izmir, Turkey Objective: Tumor budding is recognised as a poor prognostic marker in carcinomas, however there are few studies about oral cavity (OC). The prognostic importance of budding is evaluated in a series of OC squamous cell carcinomas (SCC) in this study. Study Design: Tumor budding was studied in a series of fourty-seven OC SCC cases from different sites, with follow up information. The prognostic significance of budding is evaluated.
CHROMOSOMAL INSTABILITY IN ORAL POTENTIALLY MALIGNANT DISORDERS Zuraiza Zaini, Edward Odell, Mahvash Tavassoli, Clinical and Diagnostic Sciences, Dental Institute, King’s College London, UK Objective: To compare potential routine diagnostic tests for chromosomal instability (CI) in oral epithelial dysplasia. Study Design: A gene signature based on copy number changes detected by SNP-chip analysis was tested on 20 oral dysplastic lesions with known ploidy status (7 diploid, 13 aneuploid) by QuantiGeneÒ Plex DNA Assay (Affymetrix) and qPCR (Applied Biosystems) using matched normal tissue and cell lines as control. FISH was performed to evaluate CI in chromosomes 3, 7 and 8 using a subset of targets. Results: Neither QuantiGeneÒPlex or qPCR accurately measured CI, being dependent on housekeeping gene used for normalisation. Only FISH proved sensitive, detecting 4 out of 12 cases as aneuploid using a combination of 2 targets. Conclusions: High throughput copy number assessments were unable to assess CI in oral dysplasia. FISH successfully detected CI, but at lower sensitivity than image-based DNA ploidy analysis. Keywords: Oral potentially malignant disorders, Chromosomal instability, Ploidy analysis, FISH, QuantiGene Plex, realtime PCR
IT IS WORTH STEP SECTIONING IN MINOR SALIVARY ¨ GREN’S SYNDROME GLAND EVALUATION FOR SJO Sulen Sarioglu1, Ulku Kucuk3, Pınar Çetin2, Ismail Sarı2, Merih Birlik2, 1Dokuz Eylul University, Faculty of Medicine, Department of Pathology, Izmir, Turkey; 2Dokuz Eylul University, Faculty of Medicine, Department of Rheumatology, Izmir, Turkey; 3Izmir Tepecik Research and Training Hospital, Izmir, Turkey
ABSTRACTS Abstracts e195 Objective: In this series the value of step sectioning and plasma cell focus are revisited in a series of salivary gland biopsies. Study Design: Three step sections at 200micronmeter in between, were reeavaluated seperately, for both lymphocyte and plasma cell foci, from biopsies of fourty-three cases with a diagnosis of scleroderma and suspected of Sjögren’s Syndrome (SS). Results: Lymphocytic focus was identified in 28(60,2%) cases. If the highest focus score among the three sections was considered, focus score was >¼1 in 24(85,71%) cases. Considering both focus scores are diagnostic for SS, the sections with the lowest scores would change the diagnostic category in 11(39,3%) of the cases and the lowest focus score was 0 in 11 cases (39,3%). Plasma cell focus was observed in 6(13,9%) cases, however all had a focus score higher than 1. Conclusions: These results highlight the importance of step sectioning of the minor salivary gland biopsies. Keywords: Sjögren’s Syndrome, step sectioning, minor salivary gland biopsy, plasma cell foci.
RETROSPECTIVE STUDY OF TRIGEMINAL NEURALGIA AT FACULTY OF DENTISTRY, CHULALONGKORN UNIVERSITY Kanokporn Bhalang, Department of Oral Medicine, Chulalongkorn University, Bangkok, Thailand Objective: The development of pain in orofacial locations stems from a variety of disorders. The most common neuropathic pain condition in the orofacial area is trigeminal neuralgia. Study Design: This study retrospectively examined 149 trigeminal neuralgia patients at the Department of Oral Medicine, Faculty of Dentistry, Chulalongkorn University. Results: The patients’ age range was 20-85 years old. The ratio of females to males was two to one. The most common trigger zone was located at the lower right posterior teeth. One third of the patients had dental treatments performed in the area of chief complaint prior to their visits to the school. Carbamazepine was mostly used for this disorder. The lowest dose that effectively controlled the pain was 200 mg per day. The side effects of the drug were vertigo, nausea, vomiting and decreased white blood cell counts. Conclusions: Carbamazepine was the drug of choice for trigeminal neuralgia. Keywords: Trigeminal neuralgia, carbamazepine, retrospective
TUMOR BUDDING IN ORAL CAVITY CARCINOMAS Sulen Sarioglu1, Oguz Cetinayak2, Barbaros Aydın2, Ersoy Dogan3, Fadime Akman2, Ahmet Omer Ikiz3, 1Dokuz Eylul University, Faculty of Medicine, Department of Pathology, Izmir, Turkey; 2Dokuz Eylul University, Faculty of Medicine, Department of Radiation Oncology, Izmir, Turkey; 3 Dokuz Eylul University, Faculty of Medicine, Department of Otorhinolaryngology Head and Neck Surgery, Izmir, Turkey Objective: Tumor budding is recognised as a poor prognostic marker in carcinomas, however there are few studies about oral cavity (OC). The prognostic importance of budding is evaluated in a series of OC squamous cell carcinomas (SCC) in this study. Study Design: Tumor budding was studied in a series of fourty-seven OC SCC cases from different sites, with follow up information. The prognostic significance of budding is evaluated.