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Measurement of fluorescence intensity
144
TRYAPNOSOMIASIS SEMINAR
Measurement of fluorescence intensity BY
B. WEITZ Lister Institute of Preventive Medicine Cellulose acetate membrane discs were used as a supporting matrix for the reagents employed in the fluorescent antibody technique (TousSAINT and ANDERSON, 1965). This allowed the accurate measurement of the fluorescence intensity obtained in various tests. The nature of the membrane and concentration of fluorescent antibody used in these experiments influenced the results of the test. These studies were made as a model to investigate the nature of the reaction with various living and fixed tissues. REFERENCE TOUSSAINT, A. J. & ANDERSON, R. I.
(1965). Applied Microbiology, 13,
552.
Phospholipids of Trypanosoma lewisi, T. vivax, T. congolense and T. brucei BY
D. G. GODFREY Lister Institute, Chelsea Bridge Road, London, S.W. 1 The work was carried out at the Nigerian Institute of Trypanosomiasis Research, Worn.
The phospholipids of trypanosomes were separated by thin-layer chromatography, and the phosphorus in each was estimated. T. lewisi was found to have less sphingomyelin phosphorus and more lecithin phosphorus than any of the other trypanosomes. It also had more phosphatidyl ethanolamine phosphorus than T. congolense. T. congolense differed from T. vivax and T. brucei in having a higher lecithin phosphorus content. One isolation of T. vivax was markedly different from other isolations of the same species in its high sphingomyelin and low lecithin phosphorus. Some intraspecific variation also occurred in the phospholipids among the isolations of T. brucei.
ENTOMOLOGY A possible genetic method for the control of Glossina BY
C. F. C U R T I S
Institute of Animal Genetics, Edinburgh This contribution consists only of theoretical ideas, since the practical work is only just beginning. It is well known that, in many organisms, individuals heterozygous for a chromosomal translocation are generally fully viable but are semi-sterile, because meiosis produces a proportion of gametes with unbalanced chromosome sets. In some cases, homozygotes for a translocation are found to be viable and fertile. It seems possible
TRYAPNOSOMIASIS SEMINAR
Measurement of fluorescence intensity BY
B. WEITZ Lister Institute of Preventive Medicine Cellulose acetate membrane discs were used as a supporting matrix for the reagents employed in the fluorescent antibody technique (TousSAINT and ANDERSON, 1965). This allowed the accurate measurement of the fluorescence intensity obtained in various tests. The nature of the membrane and concentration of fluorescent antibody used in these experiments influenced the results of the test. These studies were made as a model to investigate the nature of the reaction with various living and fixed tissues. REFERENCE TOUSSAINT, A. J. & ANDERSON, R. I.
(1965). Applied Microbiology, 13,
552.
Phospholipids of Trypanosoma lewisi, T. vivax, T. congolense and T. brucei BY
D. G. GODFREY Lister Institute, Chelsea Bridge Road, London, S.W. 1 The work was carried out at the Nigerian Institute of Trypanosomiasis Research, Worn.
The phospholipids of trypanosomes were separated by thin-layer chromatography, and the phosphorus in each was estimated. T. lewisi was found to have less sphingomyelin phosphorus and more lecithin phosphorus than any of the other trypanosomes. It also had more phosphatidyl ethanolamine phosphorus than T. congolense. T. congolense differed from T. vivax and T. brucei in having a higher lecithin phosphorus content. One isolation of T. vivax was markedly different from other isolations of the same species in its high sphingomyelin and low lecithin phosphorus. Some intraspecific variation also occurred in the phospholipids among the isolations of T. brucei.
ENTOMOLOGY A possible genetic method for the control of Glossina BY
C. F. C U R T I S
Institute of Animal Genetics, Edinburgh This contribution consists only of theoretical ideas, since the practical work is only just beginning. It is well known that, in many organisms, individuals heterozygous for a chromosomal translocation are generally fully viable but are semi-sterile, because meiosis produces a proportion of gametes with unbalanced chromosome sets. In some cases, homozygotes for a translocation are found to be viable and fertile. It seems possible