- Email: [email protected]
Mechanochemical phytopreparations and microorganisms in restoration of disturbed soils
Materials Today: Proceedings xxx (xxxx) xxx
Contents lists available at ScienceDirect
Materials Today: Proceedings journal homepage: www.elsevier.com/locate/matpr
Mechanochemical phytopreparations and microorganisms in restoration of disturbed soils I.D. Grodnitskaya a,b,⇑, O.E. Kondakova a, O.I. Lomovsky c, G.I. Antonov a, M.K. Meteleva a a
Sukachev Institute of Forest Siberian Branch Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk 660036, Russia Siberian Federal University, Svobodny Avenue 79, Krasnoyarsk 660041, Russia c Institute of Solid State Chemistry and Mechanochemistry Siberian Branch, Russian Academy of Sciences, Kutateladze 18, Novosibirsk 630128, Russia b
a r t i c l e
i n f o
Article history: Received 8 November 2019 Received in revised form 18 December 2019 Accepted 23 December 2019 Available online xxxx Keywords: Bioremediation Mechanochemical activation Phytopreparation Microbial preparations Scots pine seedlings
a b s t r a c t Phytopreparations based on plant biomass, obtained by solid-phase mechanochemical activation, and microbial preparations were effective in field studies aimed at stimulating the growth and viability of seedlings of pine (Pinus sylvestris L.). In addition, they also improved the soil microbiome composition and enzymatic activity in contaminated soils. Ó 2019 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the III All-Russian Conference (with International Participation) Hot Topics of Solid State Chemistry: From New Ideas to New Materials.
1. Introduction One of the key reasons for soil contamination is usage of pesticides in agriculture and forestry, which leads to ecological imbalance and increases soil toxicity. The physical, agrochemical, and biological properties of soils of artificial phytocenoses, such as forest nurseries, are rapidly deteriorated. This deterioration manifests itself as humus decomposition, reduction of the overall biological and enzymatic activities, and aggravation of oligotrophic changes. These factors cause death of beneficial native microbiota, weaken the antagonistic activity of soil, and increase the number of phytopathogens. Therefore, the soils lose their natural fertility and forest-growing properties [1–3]. Biological restoration of soils (bioremediation) becomes quite relevant and increasingly important because of various types of pollution that not only cause environmental degradation, but can also have an adverse effect on human health. Microorganisms and plants are the agents utilized for bioremediation. Bioremediation using microorganisms is environmentally friendly, does not generate secondary waste, and allows one to change the soil microbiome composition, thus improving the phy-
tosanitary status of soil and the quality of seedlings grown in forest nurseries. This bioremediation technique is often the only applicable method, especially if adjustments made for the soil management system have failed. Utilizing plants for soil purification and restoration is a wellproven technology of bioremediation, which is based on growing plants in contaminated and disturbed lands to absorb, translocate, and accumulate the contaminants [4]. Microbial and mechanochemical humin-containing substances are used to remove heavy metals from soils and water [5–7]. One of phytoremediation options is to use various phytopreparations (biofertilizers) enhancing soil fertility. Phytopreparations that are produced by mechanochemical activation of biomass and discussed in this study, together with microbial preparations, can become a promising option of environmentally friendly materials for improving forest nursery soils. In this connection, our objective was to study the effect of microbial antagonists and phytopreparations on soil parameters, as well as on germination of Scots pine (Pinus sylvestris Ledeb) seeds in the soil of a pilot forest nursery (Krasnoyarsk Krai, Russia). 2. Materials and methods
⇑ Corresponding author at: Sukachev Institute of Forest Siberian Branch Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russia. E-mail address: [email protected] (I.D. Grodnitskaya).
In May 2016, a field experiment was launched to evaluate the effect of pre-sowing treatment of Scots pine seeds with microbial
https://doi.org/10.1016/j.matpr.2019.12.243 2214-7853/Ó 2019 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the III All-Russian Conference (with International Participation) Hot Topics of Solid State Chemistry: From New Ideas to New Materials.
Please cite this article as: I. D. Grodnitskaya, O. E. Kondakova, O. I. Lomovsky et al., Mechanochemical phytopreparations and microorganisms in restoration of disturbed soils, Materials Today: Proceedings, https://doi.org/10.1016/j.matpr.2019.12.243
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I.D. Grodnitskaya et al. / Materials Today: Proceedings xxx (xxxx) xxx
antagonists and phytopreparations on soil biogenecity, seedling growth and development. Seeds of Scots pine (Pinus sylvestris L.) were pretreated with aqueous suspensions of antagonists (titer 107c/ml) and phytopreparation (PP) powders that had been produced by mechanochemical activation and dissolved in water [8]. The experiment variants were as follows: (1) control (H2O); (2) phytopreparation 1 (PP1) that was produced by mechanochemical pretreatment of a mixture of buckwheat hulls and 5% solid NaOH and contains melanins similar to those found in wood-decay fungi [9], the mill AGO-2 was used for treatment with activation mode 20 g, 2 min, concentration of melanins in powder product was 32%; (3) phytopreparation 2 (PP 2) that was produced by mechanochemical treatment of a ‘‘rice hulls + green tea” mixture and contains chelate silicon compounds, the same equipment and activation mode as in previous case, silicon chelates concentration 1.1% [10]; (4) phytopreparation 3 (PP3) – a ‘‘rice hulls + buckwheat hulls” mixture, the same equipment and activation mode, silicon chelates concentration 0.9%; (5) phytopreparation 4 (PP4) – a ‘‘natural humin-containing material + solid NaOH alkali”, the same equipment and activation mode, sodium humates concentration 45% [11]; (6) Trichoderma harzianum; (7) Trichoderma longibrachiatum; (8) a mixture of Trichoderma fungi (T. harzianum, T. lignorum, and T. longibrachiatum); (9) Bacillus amyloliquefaciens; and (10) a mixture of bacteria (Bacillus amyloliquefaciens, Bacillus subtillis, and Pseudamonas sp.). Germination of pine seeds in soil was evaluated one month after sowing; seedling growth and development was monitored every month during two growing seasons; and a record of living and dead plants was kept. Soil temperature, moisture content, and pH were measured every month; soil samples under pine seedlings were collected for subsequent laboratory microbiological and biochemical assays. 3. Results The experiment demonstrated that seed germination in soil depended on treatment type. The maximum number of pine seedlings was observed for the control samples (23.1%) and the seeds pretreated with T. harzianum (23.1%) and B. amyloliquefaciens (17.1%). After pine seeds had been pretreated with micromycete suspensions, their germination in soil was 16.8 and 25.3% higher than that observed after treatment with PPs and bacteria, respectively. The number of seedlings varied throughout the growing season (June through September) depending on weather conditions and pretreatment type. Even seed germination comparable to that for the control experiment was observed in the experimental settings utilizing phytopreparations (PPs) and microorganisms. However, high seedling mortality rate (5% on average) was detected one month later, in June, as the soil dried out. Seedling death induced a response from the soil microbiocenosis: the size of eco-trophic groups of microorganisms increased as confirmed by valid results of correlation analysis (r = 0.53 and 0.9). It was demonstrated that the highest mortality rate of Scots pine seedlings was observed in July in all experimental settings; no significant changes in the number of seedlings were observed during the remaining months of the growing period. By the end of the growing season, the greatest number of viable pine seedlings with respect to the control experimental setting was observed for the seeds that had been pretreated with T. harzianum, B. amyloliquefaciens, and PP1. A morphometric analysis of first-year Scots pine seedlings grown from seeds pretreated with microbial antagonists and PPs demonstrated that length of the primary root was almost identical for the seedlings after pretreatment with microbial antagonists and PPs and smaller than that in controls (on average, by 22). However, the tree root system in controls was less densely branched compared to that in the seedlings grown from seeds pretreated
with T. harzianum, B. amyloliquefaciens, and PP1. The densely branched root system can ensure better moisture and nutrient supply to a plant. Overall, the morphometric parameters of the seedlings grown from seeds pretreated with PPs and microorganisms were 1.3- to 2.5-fold higher than the control values. On average, microorganisms stimulated growth and development of pine seedlings 1.7 times more actively than PPs did. Pre-sowing treatment of seeds with B. amyloliquefaciens and T. harzianum strains had the strongest positive effect on the morphometric parameters of Scots pine seedlings, especially on root and stem lengthening, as well as on the increase in the inter-whorl distance (p < 0.05 in the Wilcoxon test). Pre-sowing treatment of pine seeds with microbial antagonists and phytopreparations had a positive effect on the total microbial count and microbial biomass content. During the growing season, the average total microbial count in the experiments with PPs and bacteria was 24% and 14.6% higher, respectively, than that in the control (especially in the case when pine seeds were treated with PP2 and a mixture of bacteria). The microbial biomass content was 7.4-fold higher than that in the control both in the beginning and in the end of the growing season. Pre-sowing treatment with antagonists had a stronger effect on the microbial biomass content than pretreatment with PPs. The microbial biomass content during the growing season was significantly increased by pre-sowing treatment with bacteria (B. amyloliquefaciens, a mixture of bacteria) and two PPs (PP1, PP4). The hydrothermal conditions (temperature1, moisture content2, and pH3) of soil was found to have a statistically significant effect on the average total microbial count and microbial biomass content (r1 = 071, r2 = 0.57, r3 = 0.47 and r1 = 0.6, r2 = 0.57, r3 = 0.92). Although the soil was disturbed, the eco-physiological status of microbial communities (the microbial metabolic quotient qCO2, which demonstrates a response to any changes in the soil microbiota [1,8]) in the experiments where seeds had been pretreated with microorganisms or PPs was on average lower (three- and twofold, respectively) than the control values. Thus, by the end of the growing season, the qCO2 values in the control experiment were 10.8, 36.6, and 34% higher than those after pre-sowing treatment with micromycetes, bacteria, and PPs, respectively. PP3 and T. harzianum were most efficient in restoring the ecological balance of soil microbiota. Biopreparations added to the forest nursery soil simultaneously with pine seeds had a significant effect on activity of soil enzymes. Overall, pre-sowing treatment with microorganisms (B. amyloliquefaciens and T. harzianum) enhanced activities of all enzymes (1.5- and 1.7-fold) compared to the control soil plot (the samples were collected prior to sowing). Pre-sowing treatment of seeds with PPs (PP1 and PP4 in particular) led to an especially noticeable increase in activities of invertase, polyphenoloxidase (PPO), peroxidase (PO), and urease (on average, 1.3- to 1.5-fold).
4. Conclusion Hence, having analyzed the key soil biogenecity parameters (microbial biomass content and enzyme activity) and the status of Scots pine seedlings (seed germination, the viability and quality of seedlings), we found that pre-sowing treatment with microorganisms and phytopreparations is an efficient method for bioremediation of disturbed soils. Pretreatment using T. harzianum, B. amyloliquefaciens, the phytopreparation produced by mechanochemical activation of a ‘‘buckwheat hull + 5% solid NaOH” mixture (PP1) and the phytopreparation produced by mechanochemical activation of a ‘‘natural humin-containing material + solid alkali” mixture (PP4) were the most efficient tested options. In some cases, pretreatment with mixtures of phyto- and
Please cite this article as: I. D. Grodnitskaya, O. E. Kondakova, O. I. Lomovsky et al., Mechanochemical phytopreparations and microorganisms in restoration of disturbed soils, Materials Today: Proceedings, https://doi.org/10.1016/j.matpr.2019.12.243
I.D. Grodnitskaya et al. / Materials Today: Proceedings xxx (xxxx) xxx
microbial preparations turned out to be more efficient than treatment with individual preparations. CRediT authorship contribution statement I.D. Grodnitskaya: Conceptualization, Data curation, Supervision, Writing - original draft. O.E. Kondakova: Investigation, Methodology, Formal analysis, Writing - review & editing. O.I. Lomovsky: Investigation, Methodology, Funding acquisition, Writing - review & editing. G.I. Antonov: Investigation, Methodology, Writing - review & editing. M.K. Meteleva: Methodology, Formal analysis, Investigation, Writing - review & editing. Acknowledgements The research was funded within the state assignment to ISSCM SB RAS (project No. AAAA-A17-1170303 10279-0).
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References [1] I.D. Grodnitskaya, N.D. Sorokin, Euras. Soil Sci. 40 (2007) 329–334. [2] D.Yu. Stupin, Soil pollution and the latest technologies for their restoration, Publishing House ‘‘Lan”, St. Petersburg, 2009 (in Rus.). [3] H.R. Rakhmatulloev, Lesnoy Vestnik 1 (2001) 86–90 (in Rus.). [4] L.L. Velikanov, I.I. Sidorova, Itogi Nauki i Tekhniki. Plant Protect. 6 (1988) 58– 141 (in Rus.). [5] I.D. Grodnitskaya, O.E. Kondakova, N.N. Tereschenko, Sib. J. For. Sci. 6 (2016) 13–25 (in Rus.). [6] I.S. Korotchenko, Proceedings of the international scientific conference ‘‘Problems of modern agricultural science, Krasnoyarsk (2015) 1–3 (in Rus.). [7] T. Skripkina, A. Bychkov, V. Tikhova, B. Smolyakov, O. Lomovsky, Environ. Technol. Innov. 11 (2018) 74–82. [8] O.I. Lomovsky, I.O. Lomovsky, D.V. Orlov, Green Chem. Lett. Rev. 10 (2017) 171–185. [9] O.I. Lomovsky, I.O. Lomovsky, N.V. Teplyakova, IOP Conf. Ser: Mater. Sci. Eng. 479 (1) (2019) 012010. [10] E.G. Shapolova, O.I. Lomovskii, Russ. J. Bioorg. Chem. 42 (2016) 93–98. [11] E.V. Maltseva, A.V. Savel’eva, A.A. Ivanov, N.V. Yudina, O.I. Lomovskii, Russ. J. Appl. Chem. 87 (2014) 1070–1076.
Please cite this article as: I. D. Grodnitskaya, O. E. Kondakova, O. I. Lomovsky et al., Mechanochemical phytopreparations and microorganisms in restoration of disturbed soils, Materials Today: Proceedings, https://doi.org/10.1016/j.matpr.2019.12.243
Contents lists available at ScienceDirect
Materials Today: Proceedings journal homepage: www.elsevier.com/locate/matpr
Mechanochemical phytopreparations and microorganisms in restoration of disturbed soils I.D. Grodnitskaya a,b,⇑, O.E. Kondakova a, O.I. Lomovsky c, G.I. Antonov a, M.K. Meteleva a a
Sukachev Institute of Forest Siberian Branch Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk 660036, Russia Siberian Federal University, Svobodny Avenue 79, Krasnoyarsk 660041, Russia c Institute of Solid State Chemistry and Mechanochemistry Siberian Branch, Russian Academy of Sciences, Kutateladze 18, Novosibirsk 630128, Russia b
a r t i c l e
i n f o
Article history: Received 8 November 2019 Received in revised form 18 December 2019 Accepted 23 December 2019 Available online xxxx Keywords: Bioremediation Mechanochemical activation Phytopreparation Microbial preparations Scots pine seedlings
a b s t r a c t Phytopreparations based on plant biomass, obtained by solid-phase mechanochemical activation, and microbial preparations were effective in field studies aimed at stimulating the growth and viability of seedlings of pine (Pinus sylvestris L.). In addition, they also improved the soil microbiome composition and enzymatic activity in contaminated soils. Ó 2019 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the III All-Russian Conference (with International Participation) Hot Topics of Solid State Chemistry: From New Ideas to New Materials.
1. Introduction One of the key reasons for soil contamination is usage of pesticides in agriculture and forestry, which leads to ecological imbalance and increases soil toxicity. The physical, agrochemical, and biological properties of soils of artificial phytocenoses, such as forest nurseries, are rapidly deteriorated. This deterioration manifests itself as humus decomposition, reduction of the overall biological and enzymatic activities, and aggravation of oligotrophic changes. These factors cause death of beneficial native microbiota, weaken the antagonistic activity of soil, and increase the number of phytopathogens. Therefore, the soils lose their natural fertility and forest-growing properties [1–3]. Biological restoration of soils (bioremediation) becomes quite relevant and increasingly important because of various types of pollution that not only cause environmental degradation, but can also have an adverse effect on human health. Microorganisms and plants are the agents utilized for bioremediation. Bioremediation using microorganisms is environmentally friendly, does not generate secondary waste, and allows one to change the soil microbiome composition, thus improving the phy-
tosanitary status of soil and the quality of seedlings grown in forest nurseries. This bioremediation technique is often the only applicable method, especially if adjustments made for the soil management system have failed. Utilizing plants for soil purification and restoration is a wellproven technology of bioremediation, which is based on growing plants in contaminated and disturbed lands to absorb, translocate, and accumulate the contaminants [4]. Microbial and mechanochemical humin-containing substances are used to remove heavy metals from soils and water [5–7]. One of phytoremediation options is to use various phytopreparations (biofertilizers) enhancing soil fertility. Phytopreparations that are produced by mechanochemical activation of biomass and discussed in this study, together with microbial preparations, can become a promising option of environmentally friendly materials for improving forest nursery soils. In this connection, our objective was to study the effect of microbial antagonists and phytopreparations on soil parameters, as well as on germination of Scots pine (Pinus sylvestris Ledeb) seeds in the soil of a pilot forest nursery (Krasnoyarsk Krai, Russia). 2. Materials and methods
⇑ Corresponding author at: Sukachev Institute of Forest Siberian Branch Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russia. E-mail address: [email protected] (I.D. Grodnitskaya).
In May 2016, a field experiment was launched to evaluate the effect of pre-sowing treatment of Scots pine seeds with microbial
https://doi.org/10.1016/j.matpr.2019.12.243 2214-7853/Ó 2019 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the III All-Russian Conference (with International Participation) Hot Topics of Solid State Chemistry: From New Ideas to New Materials.
Please cite this article as: I. D. Grodnitskaya, O. E. Kondakova, O. I. Lomovsky et al., Mechanochemical phytopreparations and microorganisms in restoration of disturbed soils, Materials Today: Proceedings, https://doi.org/10.1016/j.matpr.2019.12.243
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I.D. Grodnitskaya et al. / Materials Today: Proceedings xxx (xxxx) xxx
antagonists and phytopreparations on soil biogenecity, seedling growth and development. Seeds of Scots pine (Pinus sylvestris L.) were pretreated with aqueous suspensions of antagonists (titer 107c/ml) and phytopreparation (PP) powders that had been produced by mechanochemical activation and dissolved in water [8]. The experiment variants were as follows: (1) control (H2O); (2) phytopreparation 1 (PP1) that was produced by mechanochemical pretreatment of a mixture of buckwheat hulls and 5% solid NaOH and contains melanins similar to those found in wood-decay fungi [9], the mill AGO-2 was used for treatment with activation mode 20 g, 2 min, concentration of melanins in powder product was 32%; (3) phytopreparation 2 (PP 2) that was produced by mechanochemical treatment of a ‘‘rice hulls + green tea” mixture and contains chelate silicon compounds, the same equipment and activation mode as in previous case, silicon chelates concentration 1.1% [10]; (4) phytopreparation 3 (PP3) – a ‘‘rice hulls + buckwheat hulls” mixture, the same equipment and activation mode, silicon chelates concentration 0.9%; (5) phytopreparation 4 (PP4) – a ‘‘natural humin-containing material + solid NaOH alkali”, the same equipment and activation mode, sodium humates concentration 45% [11]; (6) Trichoderma harzianum; (7) Trichoderma longibrachiatum; (8) a mixture of Trichoderma fungi (T. harzianum, T. lignorum, and T. longibrachiatum); (9) Bacillus amyloliquefaciens; and (10) a mixture of bacteria (Bacillus amyloliquefaciens, Bacillus subtillis, and Pseudamonas sp.). Germination of pine seeds in soil was evaluated one month after sowing; seedling growth and development was monitored every month during two growing seasons; and a record of living and dead plants was kept. Soil temperature, moisture content, and pH were measured every month; soil samples under pine seedlings were collected for subsequent laboratory microbiological and biochemical assays. 3. Results The experiment demonstrated that seed germination in soil depended on treatment type. The maximum number of pine seedlings was observed for the control samples (23.1%) and the seeds pretreated with T. harzianum (23.1%) and B. amyloliquefaciens (17.1%). After pine seeds had been pretreated with micromycete suspensions, their germination in soil was 16.8 and 25.3% higher than that observed after treatment with PPs and bacteria, respectively. The number of seedlings varied throughout the growing season (June through September) depending on weather conditions and pretreatment type. Even seed germination comparable to that for the control experiment was observed in the experimental settings utilizing phytopreparations (PPs) and microorganisms. However, high seedling mortality rate (5% on average) was detected one month later, in June, as the soil dried out. Seedling death induced a response from the soil microbiocenosis: the size of eco-trophic groups of microorganisms increased as confirmed by valid results of correlation analysis (r = 0.53 and 0.9). It was demonstrated that the highest mortality rate of Scots pine seedlings was observed in July in all experimental settings; no significant changes in the number of seedlings were observed during the remaining months of the growing period. By the end of the growing season, the greatest number of viable pine seedlings with respect to the control experimental setting was observed for the seeds that had been pretreated with T. harzianum, B. amyloliquefaciens, and PP1. A morphometric analysis of first-year Scots pine seedlings grown from seeds pretreated with microbial antagonists and PPs demonstrated that length of the primary root was almost identical for the seedlings after pretreatment with microbial antagonists and PPs and smaller than that in controls (on average, by 22). However, the tree root system in controls was less densely branched compared to that in the seedlings grown from seeds pretreated
with T. harzianum, B. amyloliquefaciens, and PP1. The densely branched root system can ensure better moisture and nutrient supply to a plant. Overall, the morphometric parameters of the seedlings grown from seeds pretreated with PPs and microorganisms were 1.3- to 2.5-fold higher than the control values. On average, microorganisms stimulated growth and development of pine seedlings 1.7 times more actively than PPs did. Pre-sowing treatment of seeds with B. amyloliquefaciens and T. harzianum strains had the strongest positive effect on the morphometric parameters of Scots pine seedlings, especially on root and stem lengthening, as well as on the increase in the inter-whorl distance (p < 0.05 in the Wilcoxon test). Pre-sowing treatment of pine seeds with microbial antagonists and phytopreparations had a positive effect on the total microbial count and microbial biomass content. During the growing season, the average total microbial count in the experiments with PPs and bacteria was 24% and 14.6% higher, respectively, than that in the control (especially in the case when pine seeds were treated with PP2 and a mixture of bacteria). The microbial biomass content was 7.4-fold higher than that in the control both in the beginning and in the end of the growing season. Pre-sowing treatment with antagonists had a stronger effect on the microbial biomass content than pretreatment with PPs. The microbial biomass content during the growing season was significantly increased by pre-sowing treatment with bacteria (B. amyloliquefaciens, a mixture of bacteria) and two PPs (PP1, PP4). The hydrothermal conditions (temperature1, moisture content2, and pH3) of soil was found to have a statistically significant effect on the average total microbial count and microbial biomass content (r1 = 071, r2 = 0.57, r3 = 0.47 and r1 = 0.6, r2 = 0.57, r3 = 0.92). Although the soil was disturbed, the eco-physiological status of microbial communities (the microbial metabolic quotient qCO2, which demonstrates a response to any changes in the soil microbiota [1,8]) in the experiments where seeds had been pretreated with microorganisms or PPs was on average lower (three- and twofold, respectively) than the control values. Thus, by the end of the growing season, the qCO2 values in the control experiment were 10.8, 36.6, and 34% higher than those after pre-sowing treatment with micromycetes, bacteria, and PPs, respectively. PP3 and T. harzianum were most efficient in restoring the ecological balance of soil microbiota. Biopreparations added to the forest nursery soil simultaneously with pine seeds had a significant effect on activity of soil enzymes. Overall, pre-sowing treatment with microorganisms (B. amyloliquefaciens and T. harzianum) enhanced activities of all enzymes (1.5- and 1.7-fold) compared to the control soil plot (the samples were collected prior to sowing). Pre-sowing treatment of seeds with PPs (PP1 and PP4 in particular) led to an especially noticeable increase in activities of invertase, polyphenoloxidase (PPO), peroxidase (PO), and urease (on average, 1.3- to 1.5-fold).
4. Conclusion Hence, having analyzed the key soil biogenecity parameters (microbial biomass content and enzyme activity) and the status of Scots pine seedlings (seed germination, the viability and quality of seedlings), we found that pre-sowing treatment with microorganisms and phytopreparations is an efficient method for bioremediation of disturbed soils. Pretreatment using T. harzianum, B. amyloliquefaciens, the phytopreparation produced by mechanochemical activation of a ‘‘buckwheat hull + 5% solid NaOH” mixture (PP1) and the phytopreparation produced by mechanochemical activation of a ‘‘natural humin-containing material + solid alkali” mixture (PP4) were the most efficient tested options. In some cases, pretreatment with mixtures of phyto- and
Please cite this article as: I. D. Grodnitskaya, O. E. Kondakova, O. I. Lomovsky et al., Mechanochemical phytopreparations and microorganisms in restoration of disturbed soils, Materials Today: Proceedings, https://doi.org/10.1016/j.matpr.2019.12.243
I.D. Grodnitskaya et al. / Materials Today: Proceedings xxx (xxxx) xxx
microbial preparations turned out to be more efficient than treatment with individual preparations. CRediT authorship contribution statement I.D. Grodnitskaya: Conceptualization, Data curation, Supervision, Writing - original draft. O.E. Kondakova: Investigation, Methodology, Formal analysis, Writing - review & editing. O.I. Lomovsky: Investigation, Methodology, Funding acquisition, Writing - review & editing. G.I. Antonov: Investigation, Methodology, Writing - review & editing. M.K. Meteleva: Methodology, Formal analysis, Investigation, Writing - review & editing. Acknowledgements The research was funded within the state assignment to ISSCM SB RAS (project No. AAAA-A17-1170303 10279-0).
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References [1] I.D. Grodnitskaya, N.D. Sorokin, Euras. Soil Sci. 40 (2007) 329–334. [2] D.Yu. Stupin, Soil pollution and the latest technologies for their restoration, Publishing House ‘‘Lan”, St. Petersburg, 2009 (in Rus.). [3] H.R. Rakhmatulloev, Lesnoy Vestnik 1 (2001) 86–90 (in Rus.). [4] L.L. Velikanov, I.I. Sidorova, Itogi Nauki i Tekhniki. Plant Protect. 6 (1988) 58– 141 (in Rus.). [5] I.D. Grodnitskaya, O.E. Kondakova, N.N. Tereschenko, Sib. J. For. Sci. 6 (2016) 13–25 (in Rus.). [6] I.S. Korotchenko, Proceedings of the international scientific conference ‘‘Problems of modern agricultural science, Krasnoyarsk (2015) 1–3 (in Rus.). [7] T. Skripkina, A. Bychkov, V. Tikhova, B. Smolyakov, O. Lomovsky, Environ. Technol. Innov. 11 (2018) 74–82. [8] O.I. Lomovsky, I.O. Lomovsky, D.V. Orlov, Green Chem. Lett. Rev. 10 (2017) 171–185. [9] O.I. Lomovsky, I.O. Lomovsky, N.V. Teplyakova, IOP Conf. Ser: Mater. Sci. Eng. 479 (1) (2019) 012010. [10] E.G. Shapolova, O.I. Lomovskii, Russ. J. Bioorg. Chem. 42 (2016) 93–98. [11] E.V. Maltseva, A.V. Savel’eva, A.A. Ivanov, N.V. Yudina, O.I. Lomovskii, Russ. J. Appl. Chem. 87 (2014) 1070–1076.
Please cite this article as: I. D. Grodnitskaya, O. E. Kondakova, O. I. Lomovsky et al., Mechanochemical phytopreparations and microorganisms in restoration of disturbed soils, Materials Today: Proceedings, https://doi.org/10.1016/j.matpr.2019.12.243