- Email: [email protected]
Methods for sequecing large nucleic acid segments and compositions for large sequencing projects (K Hagiwara et C Harris, US)
> Programmed sequential mutagenesis (DK Gifford,
us) Massachusetts Institute of Technology and the inventor N° WO 97/46670, POT.
Methods and products are provided for the oligonucleotide-mediated polymcrase-dependent mutation of template nucleic acid sequences in a programmed sequence over multiple rounds of nuc[eic acid synthesis. The methods employ series of mutagenic primers, all of which may be present in the initial reaction system , m w'hich at least some of The mutagenic primers do not bind to or promote polylnerizatiun from the original template nucleic acids, but do bind to and promote polymerization from the mutagenized products of earlier rounds of the program of sequential mutagenesis [...) > Enhancement of gene expression (CR Drake et a/, RU) Zeneca Ltd and the inventors N° WO 97/46690. PCT
A method for enhancing the expression of a selected gene in an organism while avoiding or reducing co-suppression involves the synthesis of a I ) N A which is altered in nucleotidc sequence and is capahle of expression of a protein, ideally identical to that of a protein already expressed by a DNA alreaday present in the organism (...) I> ntegration cassette for improvment of transgenesis in eukaryotes (M Gagne et al, CA) Universite Laval N° WO 97/46691, PCT.
The present invention relates to I)NA constructs provided to improve transgenesis or genomic integration of D N A fragment of interest mto nuclear DNA of eukaryotic cell (...) > Nematode-inducible plant gene promoter (SA Ohl et al, NL) M o g e n International NV and the inventors N° WO 97/46692. POT.
The invention provides DNA fragments obtainable 44 BIOFUTLJR176 • Mars 1998
trom Arahidol~sis thaliana that is capable of p r o m o ting root knot and cyst nem a t o d e - i n d u c i b l e transcription of an associated I)NA sequence when re-introduced into a plant, and the use of said DNA fragen/ents .
8ioca~|eurs
U n i v e r s i t y of A l b e r t a a n d the inventors N° WO 97/46706, PCT
The invention provides me thods for the identification of members of a malignant lymphocyte ckme by analysis of clonntypic DNA rearrangements of T cell or B cell receptor genes (...)
Divers > Electrochemicals biosensors (AL Usala, US) Encetle Inc and the inventor N° WO 97/43633, PCT.
.\n electrochemical biosensot for determining the lexcl of a target chemical in ~ biological fluid includes ~1 electr~chemical system including an enzyme subStlate which reacts with the target chemical ro vield a sx stem signal related to the onccntration in rile biolog.ical fluid of said target ~heroical (...) .-, Enzymatic-electrochemical one-shot affinity sensor for the quantitative determination of analytes in aqueous media and affinity assay (B Gr0ndig et al, DE) S e n s l a b G e s e l l s c h a f t zur E n t w i c k l u n d und Herstellung Bioletktrochemischer Sensoren MbH, U F Z - U m weltforschungszentrum L e i p z i g - H a l l e G m b H and the inventors N° WO 97/'45738, PC-.
The in~ention relates to an ,.nzvmati,.-electrochemical affinity sensor and a one~,hot affinity assav for the quantitative determination Of ;analvtcs in aqtlcOUs media (...)
Analyse du
gen0me
> Identification of differentially methylated and mutated nucleic acids (HP
Ouffy, US) North Shore University Hospital Research Corp N° WO 97/45560. PCT.
-['hc present invention prn,'ides Methyl-(or Mutant-) i)ifferential 1)isplay (N11)I)) methods and nucleic acid probcs for detecting mutations and the mcthylation patterns of nucleic acids {...) > Methods for detection of rearranged DNA (LM Pilarski et al, CA)
> Conjuges d'un oligonucleotide/polymere conducteur el(~ctronique avec une molecule d'int(~r6t, et leurs utilisations (J Marchand et H Bazin, FR) Cis Bio International
> Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast (S M~iller and H Dalb~lge, DK} N o v o Nordisk A/S and the inventors N° WO 97/44470, PCT
Novel veast promoters for either t~,Fl-alpha protein or ribosomal protein $7 gene suitable for expression cloning in yeast and heterologous expression of proteins m yeast. The yeast prolnoters are preferably active in the pH range 4-.l I without peptone and obtained from the veast strain "Yin'rou'm h-
polytica.
N° 2750136, FR
> Proc(~d6 d'analyse d'acides nucl(Hques par hybridation et dispositif pour sa mise en o~uvre (ML Fontanel et a/, FR)
> Identification of target bacteria by fluorescence detection of primer directed amplification products (W Barbour et S Tseng,
us)
Appligene-Oncor N° 2750504, EP.
El DuPont de Nemours and Co and the inventors N° WO 97/44486, PCT.
> Method for screening compounds which interact with the L-enantiomer of a target RNA (B Metcalf et al, US, RU)
The invention describes a method for the identification of a target bacteria from a sample isolated frorl] a c o m p l e x matrix such as food and enriched for the presence of the target. The method employs a method of picking colonies cultured from the sample such that the DNA from the cells may be immediately subjected to primer directed amplification after picking and without further processing (...)
S m i t h K l i n e B e e c h a m PIc, SmithKline Beecham Corp and the inventors N° WO 97/43444, PCT. A method of identifying
conlpounds which interact, directly or indirectly, with a target molecule of interest, v~helein the target toolecule of interest is an RNA, comprises screemng compounds for intcractinn with the (L)-enantiomer of the said RNA, and indentitying, as hits, the enantiomers of those c o m p o u n d s for which said interaction is detected.
> Method for detection of mutations {M Hasebe et al, JP) Pharmacia Biotech AB N° WO 97/45555, POT.
A method and a kit for detecting a nlntation {FOre at
> Methods for sequecing large nucleic acid segments and compositions for large sequencing projects (K Hagiwara et C Harris, US)
non-attenuated sequente of a target polynucleotide in a sample, by using a mismatch binding protein (...)
The G o v e r n m e n t of t h e United States of America, the Secretary D e p a r t m e n t of Health and Human Services and the inventors N~' WO 97/43449, PCT
> Electrode configuration for matrix addressing of a molecular detection device (R Ackley, US)
Methods for rapidly se,4uencing large nucleic acids by making overlapping fragments and anchored PCR amplification are provided ,:...)
M o t o r o l a Inc and the inventor N° WO 97/45557, PCT.
> Electrochemical and photochemical electrodes and their use (I Willner et al, It_} Y i s s u m R e s e a r c h Deve-
us) Massachusetts Institute of Technology and the inventor N° WO 97/46670, POT.
Methods and products are provided for the oligonucleotide-mediated polymcrase-dependent mutation of template nucleic acid sequences in a programmed sequence over multiple rounds of nuc[eic acid synthesis. The methods employ series of mutagenic primers, all of which may be present in the initial reaction system , m w'hich at least some of The mutagenic primers do not bind to or promote polylnerizatiun from the original template nucleic acids, but do bind to and promote polymerization from the mutagenized products of earlier rounds of the program of sequential mutagenesis [...) > Enhancement of gene expression (CR Drake et a/, RU) Zeneca Ltd and the inventors N° WO 97/46690. PCT
A method for enhancing the expression of a selected gene in an organism while avoiding or reducing co-suppression involves the synthesis of a I ) N A which is altered in nucleotidc sequence and is capahle of expression of a protein, ideally identical to that of a protein already expressed by a DNA alreaday present in the organism (...) I> ntegration cassette for improvment of transgenesis in eukaryotes (M Gagne et al, CA) Universite Laval N° WO 97/46691, PCT.
The present invention relates to I)NA constructs provided to improve transgenesis or genomic integration of D N A fragment of interest mto nuclear DNA of eukaryotic cell (...) > Nematode-inducible plant gene promoter (SA Ohl et al, NL) M o g e n International NV and the inventors N° WO 97/46692. POT.
The invention provides DNA fragments obtainable 44 BIOFUTLJR176 • Mars 1998
trom Arahidol~sis thaliana that is capable of p r o m o ting root knot and cyst nem a t o d e - i n d u c i b l e transcription of an associated I)NA sequence when re-introduced into a plant, and the use of said DNA fragen/ents .
8ioca~|eurs
U n i v e r s i t y of A l b e r t a a n d the inventors N° WO 97/46706, PCT
The invention provides me thods for the identification of members of a malignant lymphocyte ckme by analysis of clonntypic DNA rearrangements of T cell or B cell receptor genes (...)
Divers > Electrochemicals biosensors (AL Usala, US) Encetle Inc and the inventor N° WO 97/43633, PCT.
.\n electrochemical biosensot for determining the lexcl of a target chemical in ~ biological fluid includes ~1 electr~chemical system including an enzyme subStlate which reacts with the target chemical ro vield a sx stem signal related to the onccntration in rile biolog.ical fluid of said target ~heroical (...) .-, Enzymatic-electrochemical one-shot affinity sensor for the quantitative determination of analytes in aqueous media and affinity assay (B Gr0ndig et al, DE) S e n s l a b G e s e l l s c h a f t zur E n t w i c k l u n d und Herstellung Bioletktrochemischer Sensoren MbH, U F Z - U m weltforschungszentrum L e i p z i g - H a l l e G m b H and the inventors N° WO 97/'45738, PC-.
The in~ention relates to an ,.nzvmati,.-electrochemical affinity sensor and a one~,hot affinity assav for the quantitative determination Of ;analvtcs in aqtlcOUs media (...)
Analyse du
gen0me
> Identification of differentially methylated and mutated nucleic acids (HP
Ouffy, US) North Shore University Hospital Research Corp N° WO 97/45560. PCT.
-['hc present invention prn,'ides Methyl-(or Mutant-) i)ifferential 1)isplay (N11)I)) methods and nucleic acid probcs for detecting mutations and the mcthylation patterns of nucleic acids {...) > Methods for detection of rearranged DNA (LM Pilarski et al, CA)
> Conjuges d'un oligonucleotide/polymere conducteur el(~ctronique avec une molecule d'int(~r6t, et leurs utilisations (J Marchand et H Bazin, FR) Cis Bio International
> Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast (S M~iller and H Dalb~lge, DK} N o v o Nordisk A/S and the inventors N° WO 97/44470, PCT
Novel veast promoters for either t~,Fl-alpha protein or ribosomal protein $7 gene suitable for expression cloning in yeast and heterologous expression of proteins m yeast. The yeast prolnoters are preferably active in the pH range 4-.l I without peptone and obtained from the veast strain "Yin'rou'm h-
polytica.
N° 2750136, FR
> Proc(~d6 d'analyse d'acides nucl(Hques par hybridation et dispositif pour sa mise en o~uvre (ML Fontanel et a/, FR)
> Identification of target bacteria by fluorescence detection of primer directed amplification products (W Barbour et S Tseng,
us)
Appligene-Oncor N° 2750504, EP.
El DuPont de Nemours and Co and the inventors N° WO 97/44486, PCT.
> Method for screening compounds which interact with the L-enantiomer of a target RNA (B Metcalf et al, US, RU)
The invention describes a method for the identification of a target bacteria from a sample isolated frorl] a c o m p l e x matrix such as food and enriched for the presence of the target. The method employs a method of picking colonies cultured from the sample such that the DNA from the cells may be immediately subjected to primer directed amplification after picking and without further processing (...)
S m i t h K l i n e B e e c h a m PIc, SmithKline Beecham Corp and the inventors N° WO 97/43444, PCT. A method of identifying
conlpounds which interact, directly or indirectly, with a target molecule of interest, v~helein the target toolecule of interest is an RNA, comprises screemng compounds for intcractinn with the (L)-enantiomer of the said RNA, and indentitying, as hits, the enantiomers of those c o m p o u n d s for which said interaction is detected.
> Method for detection of mutations {M Hasebe et al, JP) Pharmacia Biotech AB N° WO 97/45555, POT.
A method and a kit for detecting a nlntation {FOre at
> Methods for sequecing large nucleic acid segments and compositions for large sequencing projects (K Hagiwara et C Harris, US)
non-attenuated sequente of a target polynucleotide in a sample, by using a mismatch binding protein (...)
The G o v e r n m e n t of t h e United States of America, the Secretary D e p a r t m e n t of Health and Human Services and the inventors N~' WO 97/43449, PCT
> Electrode configuration for matrix addressing of a molecular detection device (R Ackley, US)
Methods for rapidly se,4uencing large nucleic acids by making overlapping fragments and anchored PCR amplification are provided ,:...)
M o t o r o l a Inc and the inventor N° WO 97/45557, PCT.
> Electrochemical and photochemical electrodes and their use (I Willner et al, It_} Y i s s u m R e s e a r c h Deve-