mirnome in decidua: new opportunity to explore endometrium receptivity establishment and early pregnancy maintain

P-824 Wednesday, October 16, 2013 POSITIONAL CHANGES OF PELVIC ORGANS FROM 2 MONTHS TO 1 YEAR AFTER THE DELIVERY: EVALUATION WITH AN OPEN CONFIGURATION MR SYSTEM. Y. Saito,a H. Okayama,a K. Masaki,b S. Ninomiya,c Y. Endo,a S. Morikawa.a aHealth Science, Shiga University of Medical Science, Otsu, Shiga, Japan; bBiwako-Gakuin University, Higashioomi, Siga, Japan; cKyoto Koka Women’s University, Kyoto, Japan. OBJECTIVE: Tissues supporting the pelvic floor are injured by delivery. They heal during the puerperal period, but complete recovery of the supporting strength to that before the delivery is considered difficult. Regarding recovery of the injured pelvic floor, functional evaluation has been performed mainly from the point of view of urinary incontinence, but few studies have been reported the positional changes of the pelvic organs during the recovery from delivery-associated injuries. DESIGN: We have evaluated the positional changes of pelvic organs after delivery and reported that the positions of internal orifice of the uterus and urinary bladder neck were the highest at one week after the delivery and then descended until 2 months, and that the positions at 2 months were lower than those in nulliparas. MATERIALS AND METHODS: The subjects were 5 females(2638years) after vaginal delivery. In this study, we evaluated positional changes of the internal orifice of the uterus and urinary bladder neck at one year after vaginal delivery and compared those at 2 months. T2-weighted MR images of the pelvis in sagittal planes were acquired in 5 females in a resting seated position employing an open MR system (0.5 Tesla SIGNA SP/2, GE). The distances of the internal orifice of the uterus and urinary bladder neck from the pubococcygeal line connecting the lower end of the pubic bone and the second coccygeal bone were measured. RESULTS: The median (Me) distance of the internal orifice of the uterus were 39.0 and 40.5 mm at 2 months and one year after delivery, respectively, showing no significant difference. The Me distance of the neck of the urinary bladder were 16.5 and 19.9 mm, respectively, which also showed no significant difference. CONCLUSION: The positions of the pelvic organs did not change from 2 months to 1 year after vaginal delivery. These levels were still lower than those in nulliparas. These results suggest that the tissues supporting pelvic floor do not completely recover even at one year after the delivery.

ENDOMETRIUM P-825 Wednesday, October 16, 2013 ABSTRACT WITHDRAWN P-826 Wednesday, October 16, 2013 miRNome IN DECIDUA: NEW OPPORTUNITY TO EXPLORE ENDOMETRIUM RECEPTIVITY ESTABLISHMENT AND EARLY PREGNANCY MAINTAIN. Y. Wang,a,c Y. Lv,c L. Wang,b G. Li.c aInner Mongolia Medical University, Hohhot, Inner Mongolia, China; bInner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia, China; cInner Mongolia University, Hohhot, Inner Mongolia, China. OBJECTIVE: The studies herein establish differential miRNA profiles of decidua from normal pregnant and abortion patients.

DESIGN: The full scale of human miRNome in deciduas remains to be determined. In addition, we highlight whether miRNAs have a physiological role in modulating the expression of genes involved in endometrium receptivity establishment and early pregnancy maintain. MATERIALS AND METHODS: An in-depth analysis of miRNomes in human normal pregnant and abortion deciduas was carried out in this study. And bioinformatics tools were used to predicted targets of candidate miRNAs and the molecular networks they may affect. RESULTS: We found 4 miRNAs (miR-17-5p, miR-199-5p, miR-20a5p, miR-30e-5p) whose expression was significantly up-regulated and 6 miRNAs down-regulated(let-7a-5p, let-7f-5p, let-7g-5p, let-7e-5p, let-7d-5p, miR98-5p) in the abortion samples. Moreover, 123 novel miRNAs were discovered and bioinformatics analysis of differentially expressed known and novel miRNAs was performed.Furthermore, the Focal adhesion and VEGF signaling pathway was enriched in the pregnant phase. CONCLUSION: Our study provides miRNomes of human deciduas and suggests a role for miRNAs in regulating the expression of some endometrial receptivity candidate biomarkers in abortion decidua, thereby suppressing early pregnacy. Inner Mongolia Bureau of health Program (2010224). Supported by: National Program on Key Basic Research Project(2012CB722306)

P-827 Wednesday, October 16, 2013 PATTERN OF PREGNANCY LOSS IS DETERMINED BY EXPOSURE TO OVARIAN STIMULATION AND PRE-OVULATORY PROGESTERONE. S. T. Daneshmand,a,b F. C. Garner,a,b M. Aguirre,a C. Hudson,a B. S. Shapiro.a,b aFertility Center of Las Vegas, Las Vegas, NV; bObstetrics and Gynecology, University of Nevada School of Medicine, Las Vegas, NV. OBJECTIVE: Compare pregnancy loss patterns in fresh transfer with low, moderate, and high levels of pre-ovulatory progesterone (P4) against frozenthawed cycles without controlled ovarian stimulation (COS). DESIGN: Retrospective cohort study. MATERIALS AND METHODS: Inclusion criteria specified pregnancy after blastocyst transfer in patients <35 years of age or else using oocyte donors <35 years of age. Fresh non-donor cycles were stratified according to pre-trigger P4 level. Pregnancies in frozen-thawed nondonor cycles served as controls. Biochemical pregnancies were lost after rising hCG titers. Ectopic pregnancies included extrauterine pregnancies and pregnancies of unknown location treated as ectopic. Spontaneous abortions (SAB) were lost intrauterine pregnancies. Due to multiple comparisons, P-values <0.01 were significant (Bonferroni correction). RESULTS: Compared to the control (frozen-thawed) group, fresh cycle pregnancies had increased biochemical pregnancy loss and ectopic pregnancy rates. Pregnancies following moderate P4 level (1.0 to 1.5 ng/mL) had greater biochemical pregnancy loss rate than the control group. Pregnancies following P4R1.5 ng/mL had greater biochemical pregnancy loss and ectopic pregnancy rates than controls. Oocyte donation cycles had similar pattern of pregnancy loss as controls. CONCLUSION: In patients <35 years old, COS exposure and elevated pre-ovulatory P4 are markers of increased risk of biochemical and ectopic pregnancy, but not SAB.

TABLE 1.

All pregnancies Biochemical pregnancies (%) Ectopic pregnancies (%) Intra-uterine pregnancies SAB (%)

All fresh non-donor transfers

Fresh, P4<1.0

Fresh, 1.0%P4<1.5

Fresh, P4R1.5

Frozen-thawed (control group)

Oocyte Donation

597 99 (16.6) * 25 (4.2) * 473 59 (12.5)

246 31 (12.6) 5 (2.0) 210 26 (12.4)

203 36 (17.7) * 7 (3.4) 160 23 (14.4)

148 32 (21.6) * 13 (8.8) * 103 10 (9.7)

406 31 (7.6) 3 (0.7) 372 46 (12.4)

240 17 (7.1) 0 (0.0) 223 31 (13.9)

P4¼ pre-ovulatory serum progesterone level (ng/mL). * ¼ significantly different from control group (P<0.01).

S386

ASRM Abstracts

Vol. 100, No. 3, Supplement, September 2013