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Modulation of the IgE response by blocking membrane IgE
Abstracts S87
SATURDAY
J ALLERGY CLIN IMMUNOL VOLUME 113, NUMBER 2
251
Modulation of the IgE Response by Blocking Membrane IgE
D. Infuehr, G. Achatz; Institute for Genetics, Salzburg, AUSTRIA. RATIONALE: Striking IgE production at the root with generating antibodies against the extracellular portion of the transmembrane domain (EMPD) of IgE could be a further strategy concerning the therapy of allergic diseases. METHODS: The phage display biopanning procedure was used for selection of single chain fragments (scFv) recognizing the Extra Membrane Proximal Domain (EMPD). RESULTS: So far, we were able to isolate two specific single chain antibodies. Showing specificity in Elisa- as well as Biacore-analysis for the EMPD-region, the sequence of these single chains will be the basis for the generation of Fab-, F(ab’)2-fragments and whole antibodies, by cloning procedures. Expressed recombinant antibodies and DNA-vaccine-constructions will be used for mice immunization experiments. CONCLUSIONS: The advantage of anti-mIgE antibodies would be the inhibition of IgE secretion before sIgE production starts. Our hypothesis suggests that cross linking mIgE receptors by anti-EMPD antibodies without the appropriate T cell help leads to direct clonal deletion and/or clonal anergy of the total mIgE B cell population. Funding: FWF, ÖNB
SATURDAY
J ALLERGY CLIN IMMUNOL VOLUME 113, NUMBER 2
251
Modulation of the IgE Response by Blocking Membrane IgE
D. Infuehr, G. Achatz; Institute for Genetics, Salzburg, AUSTRIA. RATIONALE: Striking IgE production at the root with generating antibodies against the extracellular portion of the transmembrane domain (EMPD) of IgE could be a further strategy concerning the therapy of allergic diseases. METHODS: The phage display biopanning procedure was used for selection of single chain fragments (scFv) recognizing the Extra Membrane Proximal Domain (EMPD). RESULTS: So far, we were able to isolate two specific single chain antibodies. Showing specificity in Elisa- as well as Biacore-analysis for the EMPD-region, the sequence of these single chains will be the basis for the generation of Fab-, F(ab’)2-fragments and whole antibodies, by cloning procedures. Expressed recombinant antibodies and DNA-vaccine-constructions will be used for mice immunization experiments. CONCLUSIONS: The advantage of anti-mIgE antibodies would be the inhibition of IgE secretion before sIgE production starts. Our hypothesis suggests that cross linking mIgE receptors by anti-EMPD antibodies without the appropriate T cell help leads to direct clonal deletion and/or clonal anergy of the total mIgE B cell population. Funding: FWF, ÖNB