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Mutagenic effects of benzimidazole carbamate (BCM) and related pesticides
EUROPEAN ENVIRONMENTAL MUTAGEN SOCIETY
203
31 STYLES, J. A., Industrial Hygiene Research Laboratories, Cheshire (Great Britain).
Mutagenic effects of benzimidazole carbamate (BCM) and related pesticides The cytogenetic effects of BCM and some related compounds has been examined in mammalian cells in vivo and in vitro. The main effect is that of a spindle poison but there is evidence that other mutagenic effects are produced. These compounds have been examined with microbial test systems and by dominant lethal testing. The results of these tests will be presented and discussed. Abbreviation: BCM, benzimidazole carbamate.
32 SWIETLINSKA,Z., AND J. ZUK, Institute of Biochemistry and Biophysics, Polish Academ y of Science, Warsaw (Poland).
Effect of caffeine on chromosome damage induced by chemical mutagens and ionizing radiation in Vicia faba and Secale cereale The potentiating effect of caffeine on chemically induced chromosome aberrations was studied in Vicia root tips exposed to MH3 difunctional alkylating agent D E B and two monofunctional ones, E N U and methyl methanesulphonate. Aberrations induced by MH and D E B were strongly potentiated by caffeine post-treatments; the effect was noted even when the time between the mutagen and caffeine treatment was considerably prolonged. The data obtained suggest that cells exposed to these mutagens are most sensitive to caffeine during their first passage through the S-phase. Induction of aberrations by EMS and ENU were also highly potentiated bv caffeine post-treatment. However, following EMS treatment the potentiating effect of caffeine was observed already after 20 h of recovery whilst after ENU treatment this effect was delayed up to 30 h. The yields of aberrations induced by X- and 7-rays in Vicia were not significantly increased by caffeine pre- or post-treatments. This was true both for the damage induced in seeds by X- and y-rays and for chromatid-type aberrations induced by X-rays in root tips. In contrast to Vicia the yield of aberrations induced by X-rays in rye seeds was significantly increased by caffeine post-treatment. Abbreviations: DEB, diepoxybutane; EMS, ethyl methane-sulphonate; ENU, nitrosourea; MH, maleic hydrazide.
N-ethyl-N-
203
31 STYLES, J. A., Industrial Hygiene Research Laboratories, Cheshire (Great Britain).
Mutagenic effects of benzimidazole carbamate (BCM) and related pesticides The cytogenetic effects of BCM and some related compounds has been examined in mammalian cells in vivo and in vitro. The main effect is that of a spindle poison but there is evidence that other mutagenic effects are produced. These compounds have been examined with microbial test systems and by dominant lethal testing. The results of these tests will be presented and discussed. Abbreviation: BCM, benzimidazole carbamate.
32 SWIETLINSKA,Z., AND J. ZUK, Institute of Biochemistry and Biophysics, Polish Academ y of Science, Warsaw (Poland).
Effect of caffeine on chromosome damage induced by chemical mutagens and ionizing radiation in Vicia faba and Secale cereale The potentiating effect of caffeine on chemically induced chromosome aberrations was studied in Vicia root tips exposed to MH3 difunctional alkylating agent D E B and two monofunctional ones, E N U and methyl methanesulphonate. Aberrations induced by MH and D E B were strongly potentiated by caffeine post-treatments; the effect was noted even when the time between the mutagen and caffeine treatment was considerably prolonged. The data obtained suggest that cells exposed to these mutagens are most sensitive to caffeine during their first passage through the S-phase. Induction of aberrations by EMS and ENU were also highly potentiated bv caffeine post-treatment. However, following EMS treatment the potentiating effect of caffeine was observed already after 20 h of recovery whilst after ENU treatment this effect was delayed up to 30 h. The yields of aberrations induced by X- and 7-rays in Vicia were not significantly increased by caffeine pre- or post-treatments. This was true both for the damage induced in seeds by X- and y-rays and for chromatid-type aberrations induced by X-rays in root tips. In contrast to Vicia the yield of aberrations induced by X-rays in rye seeds was significantly increased by caffeine post-treatment. Abbreviations: DEB, diepoxybutane; EMS, ethyl methane-sulphonate; ENU, nitrosourea; MH, maleic hydrazide.
N-ethyl-N-