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Nuclear magnetic resonance in biochemistry: applications to enzyme systems
445
Nuclear Magnetic Resonance Spectroscopy
of Nuclei other than Protons,
edited by T. Axenrod and G. A. Webb, John WiIey and Sons, New York, London, Sydney and Toronto, 1974, pp. xii -t- 407, price &X0.00. The contents of this book are based on the proceedings of an Advanced Study Institute held in Pisa in 1972, and provide the reader with a comprehensive review of progress in the study of the NMR spectra of nucleii other than protons. This field has become an essential part of molecular spectroscopy and is indispensable in the elucidation of molecular structures. Both experimental and theoretical aspects of the subject are covered and, where appropriate, papers relating to a particular topii: are grouped together. Special emphasis is placed on such topics as NMR of paramagnetic systems, Fourier Transform spectroscopy and the theoretical treatment of NMR parameters. The book begins with a review, by E. D_ Becker, of the properties and relaxation mechanisms of nucleii other than hydrogen. Examples of other papers included are: Structural effects on the one-bond I5 N-H coupling constant (T. Axenrod), The effects of paramagnetism on NMR spectra of nucleii other than protons (G. A. Webb), Assignment techniques in l3 C NMR spectroscopy (F. W. Wehrli) and Pulse Fourier Transform NMR with metal nucleii (G. E. Maciel). The book will be of interest to biologists, physicists and all chemists because of its excellent coverage of the rapid expansion in the field of nonproton NMR. It will also be useful as supplements reading in graduate level ‘courses on molecuIar structure and NMR spectroscopy. W. T.
Nuclear Magnetic Resonance in Biochemistry: Applications to Enzyme Systems, by Raymond A, Dwek, Clarendon Press, Oxford, 1973, pp. xviii + 395, price %8-00. Advances in instrumentation have resulted in biological applications representing the most rapidly growing area of NMR at the present time. This book deals essentially with methods for the assignment of observed resonances and for extracting structural and dynamic information from them. The book begins with a brief outline of the principles of NMR with particular emphasis on relaxation phenomena. This is followed by detailed and critical accounts of specific examples from the major areas of research. These are supplemented by summaries of the possibilities and citations of the NMR technique. Chapter 4, for example, deals with extrinsic shift probes and their use in obtaining structural information from macromolecules; and chapter 5 describes proton high resolution spectra of proteins. Further chapters describe carbon-13 studies where enrichment is shown to improve the observation of resonance, and the use of fluorine-19 as a label;
446
an important feature of this method being the great sensitivity of @F chemical shifts to en~onment~ changes. The use of paramagnetic ions features prominently in the book. This section is intended to serve as a handbook on the subject for biochemists and biophyscists. There is also a chapter describing the use of spin-label probes and the information they can give about conformatiunal changes in the presence of a figand and the coordinations of metal ions. The clear presentation and up-to-date literature coverage make the book an excellent source of evocation on current progress in the field. W. J. T.
The Inorganic Chemistry of Biological Processes, by N. N. Hughes, John Wiley and Sons, London, New York, Sydney and Toronto, 19’74, pp. 304, price $4~50_ The growth of inorganic biochemistry has been accelerated with new developments in ~s~ment~ techniques, and the subject has become one of the most rapidly expanding areas in biochemical science. The material in this book includes a survey of the occurrence and role of metal ions and describes how their functions can be studied experimentally. Chapter 1 summa&es relevant material for the reader without a prior knowledge of biological subjects. Chapter 2 considers the aspects of transition metal chemistry that are particularly relevant to the study of metalloenzymes, i.e. properties throwing light on the symmetry of the metal-binding site, the nature of the binding groups and the electronic state of the metal. Further chapters deal with the roles of metal ions in nitrogen fixation, the nitrogen cycle, and as oxygen carriers. Finally, Chapter 9 is specifically concerned with the medicinal aspects of metal ions and drugs which act via chelation of metal ions. The book presents an excellent introduction to this important field and should prove most useful for students and teachers of biology, biochemist and inorganic chemistry. J. W. T,
Nuclear Magnetic Resonance Spectroscopy
of Nuclei other than Protons,
edited by T. Axenrod and G. A. Webb, John WiIey and Sons, New York, London, Sydney and Toronto, 1974, pp. xii -t- 407, price &X0.00. The contents of this book are based on the proceedings of an Advanced Study Institute held in Pisa in 1972, and provide the reader with a comprehensive review of progress in the study of the NMR spectra of nucleii other than protons. This field has become an essential part of molecular spectroscopy and is indispensable in the elucidation of molecular structures. Both experimental and theoretical aspects of the subject are covered and, where appropriate, papers relating to a particular topii: are grouped together. Special emphasis is placed on such topics as NMR of paramagnetic systems, Fourier Transform spectroscopy and the theoretical treatment of NMR parameters. The book begins with a review, by E. D_ Becker, of the properties and relaxation mechanisms of nucleii other than hydrogen. Examples of other papers included are: Structural effects on the one-bond I5 N-H coupling constant (T. Axenrod), The effects of paramagnetism on NMR spectra of nucleii other than protons (G. A. Webb), Assignment techniques in l3 C NMR spectroscopy (F. W. Wehrli) and Pulse Fourier Transform NMR with metal nucleii (G. E. Maciel). The book will be of interest to biologists, physicists and all chemists because of its excellent coverage of the rapid expansion in the field of nonproton NMR. It will also be useful as supplements reading in graduate level ‘courses on molecuIar structure and NMR spectroscopy. W. T.
Nuclear Magnetic Resonance in Biochemistry: Applications to Enzyme Systems, by Raymond A, Dwek, Clarendon Press, Oxford, 1973, pp. xviii + 395, price %8-00. Advances in instrumentation have resulted in biological applications representing the most rapidly growing area of NMR at the present time. This book deals essentially with methods for the assignment of observed resonances and for extracting structural and dynamic information from them. The book begins with a brief outline of the principles of NMR with particular emphasis on relaxation phenomena. This is followed by detailed and critical accounts of specific examples from the major areas of research. These are supplemented by summaries of the possibilities and citations of the NMR technique. Chapter 4, for example, deals with extrinsic shift probes and their use in obtaining structural information from macromolecules; and chapter 5 describes proton high resolution spectra of proteins. Further chapters describe carbon-13 studies where enrichment is shown to improve the observation of resonance, and the use of fluorine-19 as a label;
446
an important feature of this method being the great sensitivity of @F chemical shifts to en~onment~ changes. The use of paramagnetic ions features prominently in the book. This section is intended to serve as a handbook on the subject for biochemists and biophyscists. There is also a chapter describing the use of spin-label probes and the information they can give about conformatiunal changes in the presence of a figand and the coordinations of metal ions. The clear presentation and up-to-date literature coverage make the book an excellent source of evocation on current progress in the field. W. J. T.
The Inorganic Chemistry of Biological Processes, by N. N. Hughes, John Wiley and Sons, London, New York, Sydney and Toronto, 19’74, pp. 304, price $4~50_ The growth of inorganic biochemistry has been accelerated with new developments in ~s~ment~ techniques, and the subject has become one of the most rapidly expanding areas in biochemical science. The material in this book includes a survey of the occurrence and role of metal ions and describes how their functions can be studied experimentally. Chapter 1 summa&es relevant material for the reader without a prior knowledge of biological subjects. Chapter 2 considers the aspects of transition metal chemistry that are particularly relevant to the study of metalloenzymes, i.e. properties throwing light on the symmetry of the metal-binding site, the nature of the binding groups and the electronic state of the metal. Further chapters deal with the roles of metal ions in nitrogen fixation, the nitrogen cycle, and as oxygen carriers. Finally, Chapter 9 is specifically concerned with the medicinal aspects of metal ions and drugs which act via chelation of metal ions. The book presents an excellent introduction to this important field and should prove most useful for students and teachers of biology, biochemist and inorganic chemistry. J. W. T,