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O.269 Transgenic pig as a prospective bone graft donor in reconstructive surgery
Oral Presentations surgical treatments have been established. Human-chimeric monoclonal antibodies bind to the extracellular receptor site to prevent binding by natural EGFR ligands (EGF and tumour growth factor alpha). Material and Methods: Multicenter studies of the last two years have been reviewed and compared. An outlook for nonsurgical treatment of maxillofacial cancers will be given. Results: The exact results in numbers will be evaluated within the next two weeks, because we are still waiting for the parameters of one trial. I will send it to you as soon as possible. Conclusion: In all trials the combined therapy with monoclonal antibodies and radiotherapy reduced the mortality without increasing the most frequently reported adverse events. EGFR inhibitors enhance the effects of conventional chemotherapy, especially in cisplatin-resistant diseases.
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agents, tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL),anti-CD95 monoclonal antibody (mAb), or medium alone. Cells were assayed for cell survival, and activation of JNK or caspase-8 by a standard method. Results: MIL6 cells positive for CD95 and TRAIL-receptor (R) failed to display caspase-8 activation or cell death following stimulation with anti-CD95 mAb or TRAIL. As expected, the both stimulants induced cell death of MIT6 cells. The MIL6 cells were also moderately resistant to cisplatin, compared with MIT6 cells. This differential sensitivity to cell death appeared to be associated with a slower kinetics of JNK activation in MIL6 cells, compared with MIT6 cells. Conclusion: SCC cells from metastatic lesion are more resistant to TRAIL or anti-CD95 mAb than those from primary nodules. These findings suggest that deregulation of signaling pathway of TRAIL-R or CD95 contributes to tumou progression including metastasis.
O.267 Quality of life in long-term oral cancer survivors: A comparison with Spanish general population norm J. Herce, A. Rollon, S. Gallana, J.M. Perez. Departamento Cirug´ıa Oral y Maxilofacial del Hospital Universitario Virgen Macarena de Sevilla, Spain Health-related quality of life (HRQL) data are becoming an important supplement to information pertaining to treatment outcome for cancer patients. The purpose of this study was to evaluate the HRQL of patients undergoing primary surgery for oral squamous cell carcinoma five years or more after treatment compared with spanish general population norms. Material and Methods: Twenty three oral cancer patients with cancer-free survival after surgery of >5 years were enrolled. HRQL was assessed with one standardized questionnaire: the SF-36 Health Survey (Short Form 36). Altogether 23 oral cancer patients (mean age 55.3 years, 82% male) were included five years after surgery. Results: The oral cancer patients’ SF-36 scores did not differ significantly from those of an age- and sex-matched sample from the Spanish normative population, except mental role and social functioning domains. The patients had better significant results than population norms (Wilcoxon test p < 0.05) in vitality and health perceptions domains. Conclusions: These results provided patient-reported evidence that oral cancer survivors lived with a similar HRQL compared with the general Spanish populations. Recruiment is ongoing and a larger cohort in the future will allow further analysis of the trends demonstrated in this cohort. O.268 Disruption of trail-receptor-mediated cell death pathway contributes to acquisition of metastatic phenotype of squamous cell carcinoma cells T. Noutomi, M. Itoh, E. Takada, J. Mizuguchi. Department of Immunology, Tokyo Medical University, Japan Introduction: Invasion or metastasis into surrounding tissues is a predominant phenotype of cancer cells. To get insights into the molecular mechanisms of tumour metastasis, we established several squamous cell carcinoma (SCC) cell lines derived from primary nodules and metastatic lesions. In the present study, we examined the sensitivity of cell lines derived from primary and metastatic lesions in response to several stimuli. Material and Methods: Human SCC lines originally established in our laboratory were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum and 100 `ıg/ml kanamycin. MIT6 (derived from primary nodules) and MIL6 (derived from metastatic lesion) cells were stimulated with chemotherapeutic
Wednesday, 13 September 2006, 14.30−15.20
Hall 4
New technologies: tissue engineering O.269 Transgenic pig as a prospective bone graft donor in reconstructive surgery G. Wyszynska-Pawelec, J. Zapala. Department of Maxillofacial Surgery of the Jagiellonian University, Krakow, Poland Experimental model for transgenic pig, with confirmed integration of human a 1,2 fucosylotransferase gene, and preliminary results of research on xenogenous bone grafts from transgenic pigs are presented. The aim of this paper is clinical, radiological, histological and immunohistochemical evaluation of changes in transgenic pig’s scapular bone after reconstruction of the defect by autogenous, homogenous and xenogenous bone graft. Material and Methods: 12 transgenic pigs were divided into three groups. In the I group (3 animals) autogenous bone graft healing was evaluated, in the II group (6 animals) xenogenous human bone graft and in the III group (3 animals) homogenous bone graft harvested from normal pig were assessed. Results: Radiological and histological examination was performed after 1, 2 and 4 weeks following bone grafting. Radiological examination of specimens of the I and II group revealed osteolytic changes after first and second week and features of bone graft healing after 4 weeks. Histological examination of the specimens after 1 week revealed vascularized granulation tissue with presence of fibroblasts, macrophages and reduced number of lymphocytes around bone graft in all experimental groups, resorption of bone graft trabeculae in the II and III groups. After 2 weeks in the I and II group fibrosis of granulation tissue and in the II group-new bone’s trabeculae were observed. Conclusion: Healing of autogenous and xenogenous bone grafts in transgenic pigs’ scapula was comparable in radiological and histological evaluation. O.270 3-D nanofibrous scaffold supports bone marrow-derived osteoprogenitor cells S. Srouji1,2 , E. Livne2 , E. Zussman3 . 1 Maxillofacial Surgery Department, Carmel Medical Center, Haifa, Israel; 2 Anatomy & Cell Biology Department, Faculty of Medicine, Technion, Haifa, Israel; 3 Faculty of Mechanical Engineering, Technion, Haifa, Israel Introduction and Objectives: Autogenous cancellous bone is considered the ideal graft material. It has osteogenic potential due
New technologies: tissue engineering
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agents, tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL),anti-CD95 monoclonal antibody (mAb), or medium alone. Cells were assayed for cell survival, and activation of JNK or caspase-8 by a standard method. Results: MIL6 cells positive for CD95 and TRAIL-receptor (R) failed to display caspase-8 activation or cell death following stimulation with anti-CD95 mAb or TRAIL. As expected, the both stimulants induced cell death of MIT6 cells. The MIL6 cells were also moderately resistant to cisplatin, compared with MIT6 cells. This differential sensitivity to cell death appeared to be associated with a slower kinetics of JNK activation in MIL6 cells, compared with MIT6 cells. Conclusion: SCC cells from metastatic lesion are more resistant to TRAIL or anti-CD95 mAb than those from primary nodules. These findings suggest that deregulation of signaling pathway of TRAIL-R or CD95 contributes to tumou progression including metastasis.
O.267 Quality of life in long-term oral cancer survivors: A comparison with Spanish general population norm J. Herce, A. Rollon, S. Gallana, J.M. Perez. Departamento Cirug´ıa Oral y Maxilofacial del Hospital Universitario Virgen Macarena de Sevilla, Spain Health-related quality of life (HRQL) data are becoming an important supplement to information pertaining to treatment outcome for cancer patients. The purpose of this study was to evaluate the HRQL of patients undergoing primary surgery for oral squamous cell carcinoma five years or more after treatment compared with spanish general population norms. Material and Methods: Twenty three oral cancer patients with cancer-free survival after surgery of >5 years were enrolled. HRQL was assessed with one standardized questionnaire: the SF-36 Health Survey (Short Form 36). Altogether 23 oral cancer patients (mean age 55.3 years, 82% male) were included five years after surgery. Results: The oral cancer patients’ SF-36 scores did not differ significantly from those of an age- and sex-matched sample from the Spanish normative population, except mental role and social functioning domains. The patients had better significant results than population norms (Wilcoxon test p < 0.05) in vitality and health perceptions domains. Conclusions: These results provided patient-reported evidence that oral cancer survivors lived with a similar HRQL compared with the general Spanish populations. Recruiment is ongoing and a larger cohort in the future will allow further analysis of the trends demonstrated in this cohort. O.268 Disruption of trail-receptor-mediated cell death pathway contributes to acquisition of metastatic phenotype of squamous cell carcinoma cells T. Noutomi, M. Itoh, E. Takada, J. Mizuguchi. Department of Immunology, Tokyo Medical University, Japan Introduction: Invasion or metastasis into surrounding tissues is a predominant phenotype of cancer cells. To get insights into the molecular mechanisms of tumour metastasis, we established several squamous cell carcinoma (SCC) cell lines derived from primary nodules and metastatic lesions. In the present study, we examined the sensitivity of cell lines derived from primary and metastatic lesions in response to several stimuli. Material and Methods: Human SCC lines originally established in our laboratory were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum and 100 `ıg/ml kanamycin. MIT6 (derived from primary nodules) and MIL6 (derived from metastatic lesion) cells were stimulated with chemotherapeutic
Wednesday, 13 September 2006, 14.30−15.20
Hall 4
New technologies: tissue engineering O.269 Transgenic pig as a prospective bone graft donor in reconstructive surgery G. Wyszynska-Pawelec, J. Zapala. Department of Maxillofacial Surgery of the Jagiellonian University, Krakow, Poland Experimental model for transgenic pig, with confirmed integration of human a 1,2 fucosylotransferase gene, and preliminary results of research on xenogenous bone grafts from transgenic pigs are presented. The aim of this paper is clinical, radiological, histological and immunohistochemical evaluation of changes in transgenic pig’s scapular bone after reconstruction of the defect by autogenous, homogenous and xenogenous bone graft. Material and Methods: 12 transgenic pigs were divided into three groups. In the I group (3 animals) autogenous bone graft healing was evaluated, in the II group (6 animals) xenogenous human bone graft and in the III group (3 animals) homogenous bone graft harvested from normal pig were assessed. Results: Radiological and histological examination was performed after 1, 2 and 4 weeks following bone grafting. Radiological examination of specimens of the I and II group revealed osteolytic changes after first and second week and features of bone graft healing after 4 weeks. Histological examination of the specimens after 1 week revealed vascularized granulation tissue with presence of fibroblasts, macrophages and reduced number of lymphocytes around bone graft in all experimental groups, resorption of bone graft trabeculae in the II and III groups. After 2 weeks in the I and II group fibrosis of granulation tissue and in the II group-new bone’s trabeculae were observed. Conclusion: Healing of autogenous and xenogenous bone grafts in transgenic pigs’ scapula was comparable in radiological and histological evaluation. O.270 3-D nanofibrous scaffold supports bone marrow-derived osteoprogenitor cells S. Srouji1,2 , E. Livne2 , E. Zussman3 . 1 Maxillofacial Surgery Department, Carmel Medical Center, Haifa, Israel; 2 Anatomy & Cell Biology Department, Faculty of Medicine, Technion, Haifa, Israel; 3 Faculty of Mechanical Engineering, Technion, Haifa, Israel Introduction and Objectives: Autogenous cancellous bone is considered the ideal graft material. It has osteogenic potential due