- Email: [email protected]
Omentectomy in small dogs undergoing peritoneal dialysis. Is it necessary?
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
same temperature and all the peritoneal catheters were straight blake. In dogs from the first batch, we experienced minimum subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 90 days. In the second batch, we experienced constant and reduced subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 90 days. In the third batch, we experienced medium subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 45 days. In the fourth batch, we experienced important subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at most 15 days. The study conclusion is that the best peritoneal catheter it is represented by the silicone peritoneal catheters with two Dacron cuffs. http://dx.doi.org/10.1016/j.jbiotec.2016.05.290 Omentectomy in small dogs undergoing peritoneal dialysis. Is it necessary? Munteanu Madalina Raluca 1,∗ , Toader Corneliu 3 , Bociu Nicolae Alexandru 1 , Balascau Stefanita Nicolae 1 , Chiotoroiu Alexandru Laurentiu 2 , Paun Sorin Constantin 3 , Ivascu Catalin 1 , Scafa Udriste Alexandru 3 , Vitalaru Bogdan Alexandru 1 1
Faculty of Veterinary Medicine Bucharest, Romania Emergency Clinical Hospital Bucharest, Romania 3 UMF Carol DAVILA BUCURESTI, Romania 2
E-mail address: raluca [email protected] (M.M. Raluca). In veterinary medicine, during peritoneal dialysis, the omentum can block the peritoneal catheter. We conducted the study on 20 dogs, aged between 10 months and 15 years, weighing between 1.5 and 8.5 kilograms, during 19 months. During the study, we created two batches. First batch received silicone peritoneal catheters with two Dacron cuffs, second batch received silicone peritoneal catheters without Dacron cuffs and all the peritoneal catheters were straight blake. In dogs from the first batch, we performed omentectomy before placing the catheter. In the second batch, we placed the peritoneal catheter without omentectomy. In dogs from the first batch, we experienced minimum subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 90 days. In the second batch, we experienced constant and reduced subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 90 days. We have had no relevant differences between the two batches and, in conclusion, in small dogs undergoing peritoneal dialysis it is not absolutely necessary to perform omentectomy. The omentectomy is time consuming, the patient needs more anaesthesia and can produce supplementary bleeding and pain.
S81
Proteolytic stability of nisin-loaded biopolymer particles Jolanta Sereikaite 1,∗ , Ruta Gruskiene 1 , Tatjana Krivorotova 2 1
Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania 2 Department of Polymer Chemistry, Vilnius University, Vilnius, Lithuania E-mail address: [email protected] (J. Sereikaite). Nisin is a known bacteriocin approved as a food additive for food preservation. It exhibits a wide spectrum antimicrobial activity against Gram-positive bacteria. Nisin is produced by Lactococcus lactis subsp. lactis. It is a cationic peptide composed of 34 amino acid residues. To protect nisin from the interaction with food components and to ensure the stability of antimicrobial peptide during food processing and storage period, nisin-loaded pectin and nisin loaded pectin-inulin particles were prepared by the complexation method. For particles preparation, three types of pectin polymer were used, i.e. pectin of high and low degree of esterification and pectic acid. The sensitivity to proteolysis of encapsulated nisin was investigated using trypsin from bovine pancreas and acid protease from Aspergillus saitoi. The increase of nisin stability against enzymatic digestion was found in the case of both proteases. The highest stabilization effect was observed using pectic acid as a coating material. Overall, nisin-loaded pectin-inulin particles were more stable against proteolytic degradation comparing with nisinloaded pectin particles. Acknowledgement: The Research Council of Lithuania, Grant No. SVE-03/2014, funded this study. http://dx.doi.org/10.1016/j.jbiotec.2016.05.292 Candidate-gene association study on an Italian cohort of patients affected by eating disorders Tommaso Beccari 1,∗ , Anna Tasegian 1 , Elisa Ferranti 1 , Rosa Trabace 2 , Luca Sapori 3 , Simonetta Marucci 3 , Samuela Cataldi 1 , Elisabetta Albi 1 , Laura Dalla Ragione 4
1
Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy 2 Department of Eating Disorders, ASP Potenza, Italy 3 Department of Eating Disorders, USL2 Terni, Italy 4 Department of Eating Disorders, USL1 Perugia, Italy E-mail address: [email protected] (T. Beccari).
http://dx.doi.org/10.1016/j.jbiotec.2016.05.291
Eating disorders (EDs) like anorexia nervosa (AN) and bulimia nervosa (BN) are complex neuropsychiatric conditions characterized by a multifactorial etiopathogenesis. The clinical diagnosis of EDs relies on clinical criteria. EDs share many clinical traits and the identification of biomarkers for an early differential diagnosis and for prognosis prediction is needed. The molecular mechanisms involved in EDs are still slightly understood, but epidemiologic studies underlined a strong influence of the genetic component. Several candidate-gene (CG) case-control association studies have tried to investigate the role of specific single nucleotide polymorphisms (SNPs) in EDs predisposition. EDs are likely to be polygenic as other complex disorders and the small sample size of the genotyped populations affected the results. The aim of this study is to replicate, in an Italian EDs cohort, the positive findings of CG studies
same temperature and all the peritoneal catheters were straight blake. In dogs from the first batch, we experienced minimum subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 90 days. In the second batch, we experienced constant and reduced subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 90 days. In the third batch, we experienced medium subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 45 days. In the fourth batch, we experienced important subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at most 15 days. The study conclusion is that the best peritoneal catheter it is represented by the silicone peritoneal catheters with two Dacron cuffs. http://dx.doi.org/10.1016/j.jbiotec.2016.05.290 Omentectomy in small dogs undergoing peritoneal dialysis. Is it necessary? Munteanu Madalina Raluca 1,∗ , Toader Corneliu 3 , Bociu Nicolae Alexandru 1 , Balascau Stefanita Nicolae 1 , Chiotoroiu Alexandru Laurentiu 2 , Paun Sorin Constantin 3 , Ivascu Catalin 1 , Scafa Udriste Alexandru 3 , Vitalaru Bogdan Alexandru 1 1
Faculty of Veterinary Medicine Bucharest, Romania Emergency Clinical Hospital Bucharest, Romania 3 UMF Carol DAVILA BUCURESTI, Romania 2
E-mail address: raluca [email protected] (M.M. Raluca). In veterinary medicine, during peritoneal dialysis, the omentum can block the peritoneal catheter. We conducted the study on 20 dogs, aged between 10 months and 15 years, weighing between 1.5 and 8.5 kilograms, during 19 months. During the study, we created two batches. First batch received silicone peritoneal catheters with two Dacron cuffs, second batch received silicone peritoneal catheters without Dacron cuffs and all the peritoneal catheters were straight blake. In dogs from the first batch, we performed omentectomy before placing the catheter. In the second batch, we placed the peritoneal catheter without omentectomy. In dogs from the first batch, we experienced minimum subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 90 days. In the second batch, we experienced constant and reduced subcutaneous leaks of peritoneal dialysis solution and the catheters were permeable for at least 90 days. We have had no relevant differences between the two batches and, in conclusion, in small dogs undergoing peritoneal dialysis it is not absolutely necessary to perform omentectomy. The omentectomy is time consuming, the patient needs more anaesthesia and can produce supplementary bleeding and pain.
S81
Proteolytic stability of nisin-loaded biopolymer particles Jolanta Sereikaite 1,∗ , Ruta Gruskiene 1 , Tatjana Krivorotova 2 1
Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania 2 Department of Polymer Chemistry, Vilnius University, Vilnius, Lithuania E-mail address: [email protected] (J. Sereikaite). Nisin is a known bacteriocin approved as a food additive for food preservation. It exhibits a wide spectrum antimicrobial activity against Gram-positive bacteria. Nisin is produced by Lactococcus lactis subsp. lactis. It is a cationic peptide composed of 34 amino acid residues. To protect nisin from the interaction with food components and to ensure the stability of antimicrobial peptide during food processing and storage period, nisin-loaded pectin and nisin loaded pectin-inulin particles were prepared by the complexation method. For particles preparation, three types of pectin polymer were used, i.e. pectin of high and low degree of esterification and pectic acid. The sensitivity to proteolysis of encapsulated nisin was investigated using trypsin from bovine pancreas and acid protease from Aspergillus saitoi. The increase of nisin stability against enzymatic digestion was found in the case of both proteases. The highest stabilization effect was observed using pectic acid as a coating material. Overall, nisin-loaded pectin-inulin particles were more stable against proteolytic degradation comparing with nisinloaded pectin particles. Acknowledgement: The Research Council of Lithuania, Grant No. SVE-03/2014, funded this study. http://dx.doi.org/10.1016/j.jbiotec.2016.05.292 Candidate-gene association study on an Italian cohort of patients affected by eating disorders Tommaso Beccari 1,∗ , Anna Tasegian 1 , Elisa Ferranti 1 , Rosa Trabace 2 , Luca Sapori 3 , Simonetta Marucci 3 , Samuela Cataldi 1 , Elisabetta Albi 1 , Laura Dalla Ragione 4
1
Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy 2 Department of Eating Disorders, ASP Potenza, Italy 3 Department of Eating Disorders, USL2 Terni, Italy 4 Department of Eating Disorders, USL1 Perugia, Italy E-mail address: [email protected] (T. Beccari).
http://dx.doi.org/10.1016/j.jbiotec.2016.05.291
Eating disorders (EDs) like anorexia nervosa (AN) and bulimia nervosa (BN) are complex neuropsychiatric conditions characterized by a multifactorial etiopathogenesis. The clinical diagnosis of EDs relies on clinical criteria. EDs share many clinical traits and the identification of biomarkers for an early differential diagnosis and for prognosis prediction is needed. The molecular mechanisms involved in EDs are still slightly understood, but epidemiologic studies underlined a strong influence of the genetic component. Several candidate-gene (CG) case-control association studies have tried to investigate the role of specific single nucleotide polymorphisms (SNPs) in EDs predisposition. EDs are likely to be polygenic as other complex disorders and the small sample size of the genotyped populations affected the results. The aim of this study is to replicate, in an Italian EDs cohort, the positive findings of CG studies