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Oncogenic induction of ARF tumor suppressor by E2Fs
OtolaryngologyHead and Neck Surgery Volume 121 Number 2
marked with a skin marker. The patient was then immediately taken to the operating room. An incision was then made 2 cm in length at the previously marked site. Using the gamma probe and careful dissection, the left superior adenoma was removed. Results: Following removal of the adenoma, ex vivo gamma probe counts on this tissue were extremely high, whereas the in vivo field counts had significantly decreased. Postoperative scanning failed to show the presence of any further parathyroid adenoma. Conclusion: Surgical treatment options for isolated parathyroid adenomas continue to evolve. Presented here is a method to remove these adenomas through a minimally invasive approach providing excellent surgical results with decreased morbidity.
41
Oncogenic Induction of ARF Tumor Suppressor by E2Fs WENDELL G YARBROUGH MD; BENJAMIN J COPELAND MD PHD; Chapel Hill NC
Scientific Posters
P215
papilla has been studied at various time periods of noise exposure and during recovery. This study explores hair cell regeneration of the chick sensory epithelium at 9 days of recovery after exposure to a pure-tone stimulus for either 4 or 24 hours. Methods: Twelve-day-old White Leghorn chicks were subjected to a 1500-Hz pure-tone stimulus at a 120-dB sound pressure level intensity for 4 or 24 hours. They were allowed to recover for a period of 9 days after the onset of noise exposure. The number of regenerated hair cells was quantified, and the damaged regions were examined. Results: The mean number of new hair cells for the 4-hour exposure group was 35. Similar analyses of the 24-hour exposure group revealed a mean number of 146 new hair cells. Conclusion: After comparing the results from previous studies, the number of new hair cells for the 4-hour exposure group appeared to be reaching a plateau at 9 days. The numbers of new cells for the 24-hour group steadily increased after the 9-day recovery period. This suggests the precursors of new hair and supporting cells are reentering the cell cycle in a fluctuating pattern.
Objectives: The pldINK4a/ARF genetic locus encodes 2 unique and independent tumor suppressor proteins. INK4a has been shown to activate retinoblastoma protein, and ARF has been shown to activate p53, two of the most important tumor suppressors in head and neck cancers. This study was designed to evaluate the role of E2F transcription factors in ARF expression in tumor cell lines. Methods: Tumor cells were infected with engineered adenovirns producing E2F1 and E2F2. Tumor cells were then assayed in time courses for ARF m R N A transcription by Northern blot, for ARF protein expression by Western blot, and for cell death by light microscopy. Results: Both E2F1 and E2F2 adenoviral infections induced ARF expression at the RNA and protein levels in a time-dependent manner. The induction of ARF transcription was noted prior to increases in ARF protein levels. Additionally, phenotypic changes in light microscopy consistent with cell death were observed in infected cells and also in a time-dependent manner. Conclusion: These studies demonstrate upstream regulation of the pl6INK4a/ARF locus by E2F transcription factors and suggest a role for ARF induction in tumor cell death, both of which may be important in progression of head and neck malignancies.
Objectives: We are reporting an interesting case of traumatic Eagle's syndrome and current trends in its incidence, pathophysiology, diagnosis, and management. Methods: We did a chart review of the case at hand. In addition, a detailed MEDLINE search was performed for a total literature review from 1909 to present. Results: A 37-year-old woman presented with left lateral oropharyngeal pain after being involved in a motor vehicle accident. She was diagnosed with traumatic Eagle's syndrome by physical examination and radiology. She underwent definitive treatment with transoral excision of the styloid process with complete resolution of symptoms. Conclusion: Traumatic Eagle's syndrome is a rare cause of vague oropharyngeal and neck pain. It is important for the otolaryngologist to be suspicious of this entity in a patient involved in a motor vehicle accident or head and neck trauma and to have a comprehensive understanding of its pathophysiology and current trends in diagnosis and management.
42
44
Hair Cell Regeneration after Periods of Intense Noise Exposure
Septic Temporomandibular Arthritis: A Case Report
PHILLIP L MASSENGILL MD; DOUGLAS A COTANCHE PHD; Tacoma WA; Boston MA
Objectives: Hair cell regeneration in the chick basilar
43
Traumatic Eagle's Syndrome: A Case Report and Literature Search SCHARUKH JALISI; DAVID A COTrRELL DMD; GREGORY A GRILLONE MD FACS; Boston MA
MARK A CRISWELL MD; GEORGE H ZALZAL MD; LENHANH P TRAN MD; Washington DC; Bethesda MD; Silver Spring MD
Objectives: This report presents a case of septic arthritis of the temporomandibular joint (TMJ) in a child, discusses the
marked with a skin marker. The patient was then immediately taken to the operating room. An incision was then made 2 cm in length at the previously marked site. Using the gamma probe and careful dissection, the left superior adenoma was removed. Results: Following removal of the adenoma, ex vivo gamma probe counts on this tissue were extremely high, whereas the in vivo field counts had significantly decreased. Postoperative scanning failed to show the presence of any further parathyroid adenoma. Conclusion: Surgical treatment options for isolated parathyroid adenomas continue to evolve. Presented here is a method to remove these adenomas through a minimally invasive approach providing excellent surgical results with decreased morbidity.
41
Oncogenic Induction of ARF Tumor Suppressor by E2Fs WENDELL G YARBROUGH MD; BENJAMIN J COPELAND MD PHD; Chapel Hill NC
Scientific Posters
P215
papilla has been studied at various time periods of noise exposure and during recovery. This study explores hair cell regeneration of the chick sensory epithelium at 9 days of recovery after exposure to a pure-tone stimulus for either 4 or 24 hours. Methods: Twelve-day-old White Leghorn chicks were subjected to a 1500-Hz pure-tone stimulus at a 120-dB sound pressure level intensity for 4 or 24 hours. They were allowed to recover for a period of 9 days after the onset of noise exposure. The number of regenerated hair cells was quantified, and the damaged regions were examined. Results: The mean number of new hair cells for the 4-hour exposure group was 35. Similar analyses of the 24-hour exposure group revealed a mean number of 146 new hair cells. Conclusion: After comparing the results from previous studies, the number of new hair cells for the 4-hour exposure group appeared to be reaching a plateau at 9 days. The numbers of new cells for the 24-hour group steadily increased after the 9-day recovery period. This suggests the precursors of new hair and supporting cells are reentering the cell cycle in a fluctuating pattern.
Objectives: The pldINK4a/ARF genetic locus encodes 2 unique and independent tumor suppressor proteins. INK4a has been shown to activate retinoblastoma protein, and ARF has been shown to activate p53, two of the most important tumor suppressors in head and neck cancers. This study was designed to evaluate the role of E2F transcription factors in ARF expression in tumor cell lines. Methods: Tumor cells were infected with engineered adenovirns producing E2F1 and E2F2. Tumor cells were then assayed in time courses for ARF m R N A transcription by Northern blot, for ARF protein expression by Western blot, and for cell death by light microscopy. Results: Both E2F1 and E2F2 adenoviral infections induced ARF expression at the RNA and protein levels in a time-dependent manner. The induction of ARF transcription was noted prior to increases in ARF protein levels. Additionally, phenotypic changes in light microscopy consistent with cell death were observed in infected cells and also in a time-dependent manner. Conclusion: These studies demonstrate upstream regulation of the pl6INK4a/ARF locus by E2F transcription factors and suggest a role for ARF induction in tumor cell death, both of which may be important in progression of head and neck malignancies.
Objectives: We are reporting an interesting case of traumatic Eagle's syndrome and current trends in its incidence, pathophysiology, diagnosis, and management. Methods: We did a chart review of the case at hand. In addition, a detailed MEDLINE search was performed for a total literature review from 1909 to present. Results: A 37-year-old woman presented with left lateral oropharyngeal pain after being involved in a motor vehicle accident. She was diagnosed with traumatic Eagle's syndrome by physical examination and radiology. She underwent definitive treatment with transoral excision of the styloid process with complete resolution of symptoms. Conclusion: Traumatic Eagle's syndrome is a rare cause of vague oropharyngeal and neck pain. It is important for the otolaryngologist to be suspicious of this entity in a patient involved in a motor vehicle accident or head and neck trauma and to have a comprehensive understanding of its pathophysiology and current trends in diagnosis and management.
42
44
Hair Cell Regeneration after Periods of Intense Noise Exposure
Septic Temporomandibular Arthritis: A Case Report
PHILLIP L MASSENGILL MD; DOUGLAS A COTANCHE PHD; Tacoma WA; Boston MA
Objectives: Hair cell regeneration in the chick basilar
43
Traumatic Eagle's Syndrome: A Case Report and Literature Search SCHARUKH JALISI; DAVID A COTrRELL DMD; GREGORY A GRILLONE MD FACS; Boston MA
MARK A CRISWELL MD; GEORGE H ZALZAL MD; LENHANH P TRAN MD; Washington DC; Bethesda MD; Silver Spring MD
Objectives: This report presents a case of septic arthritis of the temporomandibular joint (TMJ) in a child, discusses the