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Optimization Phas production from cheese whey by Azohydromonas lata
New Biotechnology · Volume 25S · September 2009
3.1.54 Challenges in microalgae biofuels
temperature at 30◦ C) was performed and as a result, maximum polymer concentration, was 2.86 g/l. doi:10.1016/j.nbt.2009.06.600
A. Fernández F.G. ∗ , J.M. Fernández-Sevilla, E. Molina Grima Department of Chemical Engineering, University of Almería, E04120 Almería, Spain
Recently the production of biofuels from photosynthetic microorganisms as microalgae has gathered great interest due to several key reasons related to environmental and sustainability aspects derived from the use of this type of microorganisms. They can take up CO2 and other contaminants directly from flue gases, do not need good quality water or land that could be useful for agricultural purposes, etc. In addition, the photosynthetic efficiency of microalgae is attained several times by the use of higher plants. On the basis of these matters, analyses from different sources have hinted that this is a highly interesting technical possibility and multiple companies have emerged in this field. However, although microalgal biotechnology has been in development from the 1960s, the knowledge about this type of microorganisms and the technology available today do not allow to implement industrial processes with this objective yet. This work reviews and summarizes the most relevant contributions in this field from the past years. In this sense, the technologies and systems currently used are revised and the viability of the derived processes is assessed. Analysis of these data revealed the major aspects to be taken into account in the design and operation of this type of processes, and specially the limits that can be achievable. From the experience of our group in the past 20 years an in-depth analysis of a semi-industrial facility is performed from both the energetic and economical point of view. The analysis of this case-study allows revealing the major challenges to be faced to achieve a process economically feasible. Finally a microalgae-based biofuel process is proposed taking into account the conclusions of the analysis performed and the key requirements to be met to accomplish economic feasibility are highlighted. doi:10.1016/j.nbt.2009.06.599
3.1.55 Optimization Phas production by Cupriavidus necator from molasses and acetate as substrate M. Sharifzadeh Baei Islamic Azad University, Ayatollah Amoli Branch, Amol, Islamic Republic of Iran
In present research we used molasses and acetate as combinational substrate.molasses permeate from sugar industry which was hydrolyzed to cleave its main carbon source, to glucose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Cupriavidus necator. The application of hydrolyzed molasses permeate turned out to be advantageous compared with the utilization of pure sugars. Therefore, fermentation under controlled conditions (250 rpm shaking rate, 15 hours for inoculum age, 60 hours fermentation time and
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3.1.56 Optimization Phas production from cheese whey by Azohydromonas lata M. Sharifzadeh Baei Department of Chemical Engineering, Noshirvani University of Technology, Babol, Islamic Republic of Iran
In present research we used whey as substrate permeate from dairy industry which was hydrolyzed to cleave its main carbon source, lactose, to glucose and galactose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Azohydromonas lata. The biosynthesis of PHA copolyesters containing 3-hydroxybutyrate (3HB). The application of hydrolyzed whey permeate turned out to be advantageous compared with the utilization of pure sugars. Therefore, fermentation under controlled conditions (250 rpm shaking rate, 15 hours for inoculum age, 60 hours fermentation time and temperature at 30◦ C) was performed and as a result, maximum polymer concentration, was 3.37 g/l. doi:10.1016/j.nbt.2009.06.601
3.1.57 The effect of acid treatment on Klebsiella oxytoca for biosorption of iron Y.T. Park Korea Institute of Science and Technology (KIST)-Gangneung Institute, Gangneung, Republic of Korea
As known iron-reduced bacteria, Klebsiella oxytoca were collected from the Acid mine drainage at Yeong-dong, Korea and were identified by 16s-rDNA for analyze sequence of bacteria. Yeongdong mine wastewater contaminated with 209.6 mg/L of Fe and 11.4 mg/L. For increasing adsorption ability on the surface of cell membrane, sulphuric acid, hydrochloric acid and nitric acid were used to activate the functional group of outer cell of Klebsiella oxytoca. Point one percentage of sulphuric was selected as the most effective acid treatment. Treatment using 0.1% sulphuric acid had the influence to increase the amount of uptake Fe and adsorption efficiency increased to 194.7 mg/L. The results from SEM and FT-IR indicates that biosorption of Fe increased with the acid treatment using 0.1% sulphuric acid doi:10.1016/j.nbt.2009.06.602
3.1.54 Challenges in microalgae biofuels
temperature at 30◦ C) was performed and as a result, maximum polymer concentration, was 2.86 g/l. doi:10.1016/j.nbt.2009.06.600
A. Fernández F.G. ∗ , J.M. Fernández-Sevilla, E. Molina Grima Department of Chemical Engineering, University of Almería, E04120 Almería, Spain
Recently the production of biofuels from photosynthetic microorganisms as microalgae has gathered great interest due to several key reasons related to environmental and sustainability aspects derived from the use of this type of microorganisms. They can take up CO2 and other contaminants directly from flue gases, do not need good quality water or land that could be useful for agricultural purposes, etc. In addition, the photosynthetic efficiency of microalgae is attained several times by the use of higher plants. On the basis of these matters, analyses from different sources have hinted that this is a highly interesting technical possibility and multiple companies have emerged in this field. However, although microalgal biotechnology has been in development from the 1960s, the knowledge about this type of microorganisms and the technology available today do not allow to implement industrial processes with this objective yet. This work reviews and summarizes the most relevant contributions in this field from the past years. In this sense, the technologies and systems currently used are revised and the viability of the derived processes is assessed. Analysis of these data revealed the major aspects to be taken into account in the design and operation of this type of processes, and specially the limits that can be achievable. From the experience of our group in the past 20 years an in-depth analysis of a semi-industrial facility is performed from both the energetic and economical point of view. The analysis of this case-study allows revealing the major challenges to be faced to achieve a process economically feasible. Finally a microalgae-based biofuel process is proposed taking into account the conclusions of the analysis performed and the key requirements to be met to accomplish economic feasibility are highlighted. doi:10.1016/j.nbt.2009.06.599
3.1.55 Optimization Phas production by Cupriavidus necator from molasses and acetate as substrate M. Sharifzadeh Baei Islamic Azad University, Ayatollah Amoli Branch, Amol, Islamic Republic of Iran
In present research we used molasses and acetate as combinational substrate.molasses permeate from sugar industry which was hydrolyzed to cleave its main carbon source, to glucose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Cupriavidus necator. The application of hydrolyzed molasses permeate turned out to be advantageous compared with the utilization of pure sugars. Therefore, fermentation under controlled conditions (250 rpm shaking rate, 15 hours for inoculum age, 60 hours fermentation time and
S268
www.elsevier.com/locate/nbt
3.1.56 Optimization Phas production from cheese whey by Azohydromonas lata M. Sharifzadeh Baei Department of Chemical Engineering, Noshirvani University of Technology, Babol, Islamic Republic of Iran
In present research we used whey as substrate permeate from dairy industry which was hydrolyzed to cleave its main carbon source, lactose, to glucose and galactose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Azohydromonas lata. The biosynthesis of PHA copolyesters containing 3-hydroxybutyrate (3HB). The application of hydrolyzed whey permeate turned out to be advantageous compared with the utilization of pure sugars. Therefore, fermentation under controlled conditions (250 rpm shaking rate, 15 hours for inoculum age, 60 hours fermentation time and temperature at 30◦ C) was performed and as a result, maximum polymer concentration, was 3.37 g/l. doi:10.1016/j.nbt.2009.06.601
3.1.57 The effect of acid treatment on Klebsiella oxytoca for biosorption of iron Y.T. Park Korea Institute of Science and Technology (KIST)-Gangneung Institute, Gangneung, Republic of Korea
As known iron-reduced bacteria, Klebsiella oxytoca were collected from the Acid mine drainage at Yeong-dong, Korea and were identified by 16s-rDNA for analyze sequence of bacteria. Yeongdong mine wastewater contaminated with 209.6 mg/L of Fe and 11.4 mg/L. For increasing adsorption ability on the surface of cell membrane, sulphuric acid, hydrochloric acid and nitric acid were used to activate the functional group of outer cell of Klebsiella oxytoca. Point one percentage of sulphuric was selected as the most effective acid treatment. Treatment using 0.1% sulphuric acid had the influence to increase the amount of uptake Fe and adsorption efficiency increased to 194.7 mg/L. The results from SEM and FT-IR indicates that biosorption of Fe increased with the acid treatment using 0.1% sulphuric acid doi:10.1016/j.nbt.2009.06.602