or abasic sites

PATENT ABSTRACTS has been identified and purified from cultured human cells. DNA sequences encoding additional heregulin polypeptides, designated heregulinalpha, heregulin- betal, heregulin- beta2, heregulin- beta2-1ike, and heregulin- beta3, have been isolated, sequenced and expressed. Provided herein are nucleic acid sequences encoding the amino acid sequences of heregulins useful in the production of heregulins by recombinant means. Further provided are the amino acid sequences of heregulins and purification methods therefor. Heregulins and their antibodies are useful as therapeutic agents and in diagnostic methods.

5367064 ALPHA-1-ANTICHYMOTRYPSIN, A N A L O G U E S AND M E T H O D S OF PRODUCTION Rubin Harve; Wang Zhi M; Cooperman Barry S; Schechter Norma - The Trustees of the University of Pennylsvania The invention provides alpha-l-antichymotrypsin and protein preparations comprising human alpha1-antichymotrypsin produced by E. coli cells transformed with a DNA sequence encoding human alpha-l-antichymotrypsin. The invention also provides methods for producing alpha-1antichymotrypsin. The invention further provides analogues of alpha-l-antichymotrypsin that exhibit antichymotrypsin, anti-trypsin and antithrombin activity and methods of producing the analogues.

5367065 C O N S T I T U T I V E TRIPLE R E S P O N S E GENE A N D MUTATIONS Ecker Joseph R; Kieber Joseph - The Trustees of the University of Pennsylvania The present invention is directed to nucleic acid sequences for constitutive triple response mutants and corresponding amino acid sequences. Several ctr mutations are included within the scope of the present invention. The nucleic acid sequences set forth in SEQUENCE ID NUMBERS 1, and 3-6 as well as amino acid sequences set forth in

89

SEQUENCE ID NUMBERS 1 and 2 are particular embodiments of the present invention.

5367066 O L I G O N U C L E O T I D E S WITH SELECTABLY CLEAVABLE A N D / O R ABASIC SITES Urdea Michael; Corporation

Horn

Thomas

Chiron

A modified polynucleotide containing at least one cleavable or abasic site as shown below. (*See Patent for Chemical Structure*) DNA1 is a first segment of DNA; DNA2 is a second segment of DNA; and Rm is C1 to C16 alkylene or an oxytheylene oligomer -(CH2CH20)z- where z is an interger in the range of 1 to 16 inclusive, and Rn is selected from the group consisting of (*See Patent for Chemical Structure*) O2N#701 +A,4 +BN,4 +SD,5 +S,5 CH+HD 2+L CH+HD 2 + A B , 4 0 + A B , 4 +M,I + A D , 0 0 + M R , 1 C+AB,4 ,+RE +0 +RB +RD,11447799ccff +BL,44 +701 +A,4 +BN,4 +SD,5 +S,5 S+AB,4 CH+HD 2+L CH+HD 2 + A B , 4 0 + A B , 4 +M,1 +AD,0 O+MR,1 C+AB,4 ,+RE +0 +RB +RD,335588bbdd00 +BL,55 +701 +A,4 +BN,4444 +A,3 +RD,3355 +A,2 +BN,d +USA +SB,14 H+A,2 +BN,3 +USA +S,5 CH+HD 2 + A B , 4 0 + M , 1 +AD,0 O+MR,1 C+AB,4 +A,3 +RD,335588bbdd00 +BL,55 +701 +A,3 +SD,26 +S,10 ,+RE +0 +RB +RD,11447799ccff +BL,44 +701 +A,4 +BN,4 +SD,5 +S,5 +M,1 +AD,0 O+MR,1 +AD,8 O+MR,1 S+AB,4 CH+HD 2+L CH+HD 2+AB,4 O+AB,4 +M,1 + A D , 0 0 + M R , 1 C+AB,4 ,+BL,4 and+RE +0 CH+HD 3+L O+AB,4 CH+HD 2+AB,4 CH+HD 2+AB,4 O+ABA CH+HD 2+AB,4. +RE+PS +RE+PS Such polynucleotides are useful in solid phase hybridizations because they permit the release of a label from the solid support after the hybridization reaction.+ REion.+ REtion reaction.+ REon.+ REon.+ RE+RE.+ RE.+ REREf a label from the solid support after the hybridization reaction.+RE+RE.+RE+RE+ RE+RE+ RERE+ RE + RE.+RE+RE.+RE+RE.+ RE+RE+ RE.+ RE+RE.+ RE+ RE.+RE+RE.+RE+ RE.+ REion.+REion.+RE 1 +AD,80+MR,1 S+AB,4 CH+HD 2+L CH+HD 2 + A B , 4 0 + A B , 4 +M,1 + A D , 0 0 + M R , 1 C+AB,4 ,+BL,4 and+RE +0 CH+HD 3+L O+ABA CH+HD 2+AB,4 CH+HD 2+AB,4 O+AB,4 CH+HD 2+AB,4 +RE+PS +RE+PS Such polynucleotides are useful in solid phase hybridizations because they permit the release of a label from the solid support after the hybridization reacfion.+RE