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P.089 YKL-40 as prognostic biomarker in patients with oral cancer
S190
Journal of Cranio-Maxillofacial Surgery 36(2008) Suppl. 1
Abstracts, EACMFS XIX Congress
P.087 Salivary vascular growth factor in oral cancer prognosis
P.089 YKL-40 as prognostic biomarker in patients with oral cancer
M. Vourvachis, T. Upile, W. Jerjes, S. Singh, C. Hopper. University College London Hospitals NHS Trust, London, UK
M. Scheer, S. Wild, A.C. Kuebler, J.E. Zoeller. University of Cologne, Cologne, Germany
Objectives: Saliva contains many biological proteins, including growth factors and cytokines. Vascular endothelial growth factor (VEGF) is a key regulator of vessel growth and regression, and acts by protecting endothelial cells from apoptosis. It is an important regulator of angiogenesis by promoting endothelial cell migration and proliferation. Several studies suggest that vascular endothelial growth factor is one of the most important cytokines responsible for the development, maintenance and progression of many cancers, by promoting angiogenesis. The aim of this study is to develop a database of values of salivary vascular endothelial growth factor (VEGF) in controls and oral cancer patients. Methods: Twenty-one participants (12 males and 9 females) of whom 14 were healthy and 7 had oral squamous cell carcinoma took part in this study. An immunoassay was used to quantify a range of specific vascular endothelial growth factors in blood and saliva. This was correlated to tumour factors and patient outcomes. Results: There were significant differences in the salivary and serum levels of the control group and the cancer group. Conclusions: We present an independent normative data of salivary VEGF in controls. We also suggest the use of this simple non-invasive test in predicting and assessing outcome in oral cancer patients.
Introduction: Recent studies have identified elevated levels of YKL-40, a serum glycoprotein as prognostic parameter in cancers of the lung, breast, prostata, ovar as well as kidney. However no prospective study in oral cancer have been conducted yet. The aim of our study was to evaluate the serum YKL-40 levels in oral cancer patients as well as in healthy volunteers. Material and Methods: Blood samples were collected prior to therapy after informed consent was given. Serum was frozen after centrifugation and stored at −80ºC. YKL-40 levels were assessed in serum by using Metra YKL-40 EIA Kit (Quidel Corporation, San Diego, California, USA) aacording to the manufacturer instructions. All values were measured in duplicates. Results: In our series YKL-40 serum values were evaluated in 56 patients (35 male and 21 female, mean 61.8 years) with oral cancer. 67.9% suffered from T1/T2 Tumors and 32.1% presented with locally advanced disease. Lymph node metastasis were detected in 9 patients (6.8%). Treatment consisted of surgery (23 patients, 41.1%) and postoperate RCT (31 patients, 55.4%). During follow up 10 patients died because their disease. The healthy volunteers consisted of 17 subjects (7 male, 10 female, mean 39.9 years). The median survival was 15.4 month for tumor recurrence and 22 month for overall survivall. YKL-40 serum values were significantly higher (p < 0.001) in oral cancer patients (mean 199.01 mikrog/L) than in healthy volunteers (mean 71.34 mikrog/L). Oral cancer patients with lymph node metastases showed significantly elevated serum values (p = 0.027). However no correlation of YKL-40 with recurrence free or overall survival could be demonstrated.
P.088 The quality of life in oncology patients A. Pradillos, S. Bescos, J. Pamias, Ja. Hueto, G. Raspall. Hospital Vall d’Hebron, Barcelona, Spain Introduction: Quality of life that is being offered to our patients is an important issue that needs to be taken into account when we schedule a surgical intervention. In oncologic patients we need to be resolutive but at the same time we need to find the less mutilant option, not just functional but also esthetical. The IV UW-QOL test version (University of Washington Quality of Life test) was considered the best technique to measure the quality of life of our oncologic patients. Material and Methods: We made a retrospective study of oncologic patients that underwent surgical interventions between 2004 and 2007. All them received some sort of mandibulectomy because of SCC in their oral cavities with bone invasion. In some of them the defect was reconstructed with a microvascularized fibular graft. No reconstruction or just soft tissue reconstruction was realized to the rest of the patients. We evaluated the quality of life of both groups with the UW-QOL test. Objectives: The goal of the study is to evaluate: (1) The quality of life of the oncologic patients that underwent surgical interventions; (2) To consider if there is a difference between the group that received a bone reconstruction and the group that just received a soft tissue one or no reconstruction at all, and to notice if there is a quality of life worsening in patients that received radiotherapy or quimiotherapy; (3) Identify the adverse effects or complications that affect the most the patients quality of life. Conclusions: Interviewed patients quality of life perception is very good overall, and in some cases even better than the one perceived before surgery. The patients that received a bone graft reconstruction feel a better result not just functional (matication, talk) but also esthetical. The factors that worry the most in both groups are the ones related with the eating process (mastication, deglution and salivation).
Facial burns and sequelae P.090 Comparative characteristic of the regenerative activity of soft tissues with abscesses and phlegmones of the face N. Korotkikh, G. Toboev, A. Morozov. VSMA, Voronezh, Russia The lingering inflammatory diseases of the maxillofacial region with complications may take place after surgical intervention. This may be considered as a failure of the regenerative processes. We have examined 58 patients (23 women and 35 men) with abscesses (26 persons) and phlegmones (32 persons) of soft tissues around the maxillofacial region. The patients were of 20 to 62 years old. The material for morphological research was taken on the day of cutting of suppurative hearth. The preparations were painted with hematoxylin and eosin, Sudan III and according to the method of Van Gizon. All parameters were defined in 50 fields of vision. The significance of the proliferative activity of soft tissues was estimated. The result was defined by the calculation of correlation of neutrophils and fibroblasts. The number of mitoses was also estimated. Besides, we studied presence of collagenic and precollagenic fibers in the side of suppurative cavity. During the study of patients preparations with abscesses the attention was attracted to the presence of the well visible net of reticulative fibers in all cases. The presence of collagenic fibers also took place in all cases, but the density of their spreading was different and corresponded to the number of mitoses. And that density was in reverse dependence on the correlation of the number of fibroblasts and neutrophils. During the study of patients preparations with phlegmones we noted frequent presence of reticulative fibers and seldom presence of collagenic fibers. This phenomenon was accompanied by small, in comparison to the first group patients, number of
Journal of Cranio-Maxillofacial Surgery 36(2008) Suppl. 1
Abstracts, EACMFS XIX Congress
P.087 Salivary vascular growth factor in oral cancer prognosis
P.089 YKL-40 as prognostic biomarker in patients with oral cancer
M. Vourvachis, T. Upile, W. Jerjes, S. Singh, C. Hopper. University College London Hospitals NHS Trust, London, UK
M. Scheer, S. Wild, A.C. Kuebler, J.E. Zoeller. University of Cologne, Cologne, Germany
Objectives: Saliva contains many biological proteins, including growth factors and cytokines. Vascular endothelial growth factor (VEGF) is a key regulator of vessel growth and regression, and acts by protecting endothelial cells from apoptosis. It is an important regulator of angiogenesis by promoting endothelial cell migration and proliferation. Several studies suggest that vascular endothelial growth factor is one of the most important cytokines responsible for the development, maintenance and progression of many cancers, by promoting angiogenesis. The aim of this study is to develop a database of values of salivary vascular endothelial growth factor (VEGF) in controls and oral cancer patients. Methods: Twenty-one participants (12 males and 9 females) of whom 14 were healthy and 7 had oral squamous cell carcinoma took part in this study. An immunoassay was used to quantify a range of specific vascular endothelial growth factors in blood and saliva. This was correlated to tumour factors and patient outcomes. Results: There were significant differences in the salivary and serum levels of the control group and the cancer group. Conclusions: We present an independent normative data of salivary VEGF in controls. We also suggest the use of this simple non-invasive test in predicting and assessing outcome in oral cancer patients.
Introduction: Recent studies have identified elevated levels of YKL-40, a serum glycoprotein as prognostic parameter in cancers of the lung, breast, prostata, ovar as well as kidney. However no prospective study in oral cancer have been conducted yet. The aim of our study was to evaluate the serum YKL-40 levels in oral cancer patients as well as in healthy volunteers. Material and Methods: Blood samples were collected prior to therapy after informed consent was given. Serum was frozen after centrifugation and stored at −80ºC. YKL-40 levels were assessed in serum by using Metra YKL-40 EIA Kit (Quidel Corporation, San Diego, California, USA) aacording to the manufacturer instructions. All values were measured in duplicates. Results: In our series YKL-40 serum values were evaluated in 56 patients (35 male and 21 female, mean 61.8 years) with oral cancer. 67.9% suffered from T1/T2 Tumors and 32.1% presented with locally advanced disease. Lymph node metastasis were detected in 9 patients (6.8%). Treatment consisted of surgery (23 patients, 41.1%) and postoperate RCT (31 patients, 55.4%). During follow up 10 patients died because their disease. The healthy volunteers consisted of 17 subjects (7 male, 10 female, mean 39.9 years). The median survival was 15.4 month for tumor recurrence and 22 month for overall survivall. YKL-40 serum values were significantly higher (p < 0.001) in oral cancer patients (mean 199.01 mikrog/L) than in healthy volunteers (mean 71.34 mikrog/L). Oral cancer patients with lymph node metastases showed significantly elevated serum values (p = 0.027). However no correlation of YKL-40 with recurrence free or overall survival could be demonstrated.
P.088 The quality of life in oncology patients A. Pradillos, S. Bescos, J. Pamias, Ja. Hueto, G. Raspall. Hospital Vall d’Hebron, Barcelona, Spain Introduction: Quality of life that is being offered to our patients is an important issue that needs to be taken into account when we schedule a surgical intervention. In oncologic patients we need to be resolutive but at the same time we need to find the less mutilant option, not just functional but also esthetical. The IV UW-QOL test version (University of Washington Quality of Life test) was considered the best technique to measure the quality of life of our oncologic patients. Material and Methods: We made a retrospective study of oncologic patients that underwent surgical interventions between 2004 and 2007. All them received some sort of mandibulectomy because of SCC in their oral cavities with bone invasion. In some of them the defect was reconstructed with a microvascularized fibular graft. No reconstruction or just soft tissue reconstruction was realized to the rest of the patients. We evaluated the quality of life of both groups with the UW-QOL test. Objectives: The goal of the study is to evaluate: (1) The quality of life of the oncologic patients that underwent surgical interventions; (2) To consider if there is a difference between the group that received a bone reconstruction and the group that just received a soft tissue one or no reconstruction at all, and to notice if there is a quality of life worsening in patients that received radiotherapy or quimiotherapy; (3) Identify the adverse effects or complications that affect the most the patients quality of life. Conclusions: Interviewed patients quality of life perception is very good overall, and in some cases even better than the one perceived before surgery. The patients that received a bone graft reconstruction feel a better result not just functional (matication, talk) but also esthetical. The factors that worry the most in both groups are the ones related with the eating process (mastication, deglution and salivation).
Facial burns and sequelae P.090 Comparative characteristic of the regenerative activity of soft tissues with abscesses and phlegmones of the face N. Korotkikh, G. Toboev, A. Morozov. VSMA, Voronezh, Russia The lingering inflammatory diseases of the maxillofacial region with complications may take place after surgical intervention. This may be considered as a failure of the regenerative processes. We have examined 58 patients (23 women and 35 men) with abscesses (26 persons) and phlegmones (32 persons) of soft tissues around the maxillofacial region. The patients were of 20 to 62 years old. The material for morphological research was taken on the day of cutting of suppurative hearth. The preparations were painted with hematoxylin and eosin, Sudan III and according to the method of Van Gizon. All parameters were defined in 50 fields of vision. The significance of the proliferative activity of soft tissues was estimated. The result was defined by the calculation of correlation of neutrophils and fibroblasts. The number of mitoses was also estimated. Besides, we studied presence of collagenic and precollagenic fibers in the side of suppurative cavity. During the study of patients preparations with abscesses the attention was attracted to the presence of the well visible net of reticulative fibers in all cases. The presence of collagenic fibers also took place in all cases, but the density of their spreading was different and corresponded to the number of mitoses. And that density was in reverse dependence on the correlation of the number of fibroblasts and neutrophils. During the study of patients preparations with phlegmones we noted frequent presence of reticulative fibers and seldom presence of collagenic fibers. This phenomenon was accompanied by small, in comparison to the first group patients, number of