Poster Sessions
178
II and PCR for HPV DNA was done. Ty-pe 16 was found and the patient was referred to oral surgery. He refused this kind of trealrnent, but in few months the lesion began to spread and surgery was preformed. Open stoma remained in the upper left quadrant, with no need for plastac surgery. Conclusion: Ichopathlc leukoplaklal of the oral cavity can arise from either keratmazed or non-keratnnzed epathehum. Most lesions follow a benign course, but some transform into squamous cell carcinoma. Proliferative verrucous leukoplakia (PVL), a high-rusk type of oral leukoplakia is unique oral white lesion m which human paplllomavtrus (HPV) may play a role. Unlike many other vtruses that reduce prohferatlve changes m these cells, winch result m both benagn and mahgnant tumors. To date, more than 70 dafferent HPV types have been isolated, with DNA types 16 and 18 having the strongest malignant potential.
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Brushing cytology in the interpretation of metastatic malignancies in the oral cavity
D. TasmolaklS2, V. ThomaldlS I, I. Tsamls i, M. Lambropoulou "~, G. Alexladls ~, N. Papadopoulos 3. ~Department of Oral
cmd M~zrtlIofactal Surgery. Regional Unteerstty Hospital of Alexandroupohs. Thrace. Greece. 2Depa~'tment of C~'topathology. Regional General Hospttal of Chama, Crete, Greece; 'Department of H~stology-Embryolog3; Democritus Untvers~(v of Thrace, FaculO' of Medtctne, Alexandroupohs. Thrace. Greece Introduction: Metastases to the oral regaon are unconmaon mad may occur m the oral soft tissues or the jawbones. Because of thetr raraty (1% of all mallgnaat oral newgrowths), they are challengang to chagnose. However, autopsies of cancer patients reveal a higher frequency of metastatic deposats in the jawbones, not clinically manifested. The most frequently reported prlmaraes are breast, lung, kidney, bones, and colorecaun. Symptoms develop m a short tmle. In the soft tassues, most lnchvaduals complain of a lump, m the jawbones swelling, pare and paresthesaa of the affected nerve are the chief complaints. In some cases, the metastasas as dasclosed m a recent extraction sate. Radaology as that of a lytac lesaon. Hastology is the comer stone for the daagnosas. Exfolaatlve oral cytology has become less amportant notably because of a high proportion of false negataves. More recently, however, the development of automated cytomorphometrlC devices, of DNA asmlyses and of technaques for the detectaon of ~ m o r n~arkers has reawakened interest m tins approach. Materials and Methods: In this study we evaluated the performance of brushing cytology with that of permanent sections hastology m twelve patients. We used dtrect smears and cell block preparations for mamunocytochermcal analyses. Results: There was a false negative result by direct smearing concerning a case of melanoma arising m the gmgava of a female patient w~th free medical Nstory. There were no false pomtwe resuRs. So positive predictive value was 100%, and negative prechctlve v a n e was 98°4. The false negative resuR was attributed to the apparent failure of a microscopic focus of metastatic deposit to adhere to the glass slide, a problem that seems unavoidable. Co~elasiom Brushing cytology as a valuable adjunct m the interpretation of oral neoplastic processes. It as performed easily and rapidly, and no tissue is lost. However knowledge of the climcal findings and past medical history is imperative.
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Withdrawn
P.1071 Oral squamous cell carcinoma around dental implants in a patient with oral lichen planusa case report R. Czerninski, G. Almoznino, A. Maly, E. Regev. The Hebrew
Untverstty- Hadassah School of dentaI medtcme. Jerusalem, Israel Introd~lefion: Oral Squamous Cell Carcinoma (OSCC) is related to rask factors such as smoking and alcohol, and with premallgnaat conditions such as Erosive Lichen Planus (ELP). Although osseo-mtegrated dental amplants have rarely been reported m assoclataon of OSCC, the questaon of the safety of placing dental implants in patients with pre-malignant lemons and risk habits has not been resolved yet. Materials and Methods: Case Report: A 52 year old female presented wath a 2.5 cm non-healing ulcer m the left lower alveolar radge, m proxmnty to a dental mlplant placed 3 years prevaously. She had been chagnosed wath ELP 5 years praor to the implant procedure, and had not ceased smoking heavaly m spate of being reformed of the rasks. Res~dts: The hlstopathologacal daagnosas was SCC. The pataent underwent a complete worknp, which faded out bone nodal or distant organ involvement, resulting m a classification of local, stage II disease, The pataent was treated by local wide resection including the adjacent alveolar bone and lpsalateral modafied neck dlssectaon. Conelus|on: The pataent had 2 risk factors for oral malagnancy, however, the tumor developed m close proxnmty to the amplaslt site and not elsewhere m the oral cavity. There may be an increased risk assocaated wath the Lrnplas~t ~tselI\ the surgical procedure or the perl-tmplant envtrorm~ent which m addataon to the pre-existing risk factors may lead to full-blown malagnancy. Further research as needed to establish the safety of dental tmplants m tugh risk patients.
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Lectin histochemistry of mucoepidermoid carcinoma
M.J.B.M. R~go 1, L.X. Santos I, L.B. Carvalho Jr. ~2, E.I.C. Beltr;~ 1,2, A.P.V. Sobral 1,3. ;LIKA Laboratorto de
Imunopatologm Ke~zo Asamt UFPE.Braztl. 2Departamento de Btoquimtca. CCB/UFPE. Bras~l, SDtsctphna de Patolog~a Bucal -Faculdade de Odontologta de Pe~7,ambuco, Brawl Introd~lefion: Mucoepadermold carcinoma (MEC) as the most
conmaon malignant sahvary tumour, classified as low, mtermedaate and t~gh grade. Regarding the pathology of salivary gland tmnottrs chscusslons related to morphology, histogenetic, diagnosis and classxficatxon have taken place specmlly to characterlze the constxtuent turnout cells to establish d~fferences among turnout types. Traditionally mmamlOhlStochen~stry has been the choice for exploiting such features but lectm t~stochennstry has been increasingly used as an auxdrary tool for dxagnosls. Lectms are ubxqtutous proteins which recogmze carbohydrates specifically and reversxbly. In this work lectms are used as tustochermstry markers for normal and transformed sahvary glands. M a t e r ~ l s and Methods: Bxopsxes of mucoepldermold carcmoma (low grade, n = l : mtermedaate grade, n = l and high grade, n = 3) were sliced (4~m), treated wxth 0.1 o~'o (w/v) trypsin and 0.3% (v/v) methanol-H202 solutions and incubated with lectins conjugated to peroxidase, HRP (Concanavalin A, Con A-HRP and Ulex europeus I, UEA-HRP). Peroxldase was VlSUahzed with a solution of daan~nobei~zldme-H202 and evaluated by hght microscopy. Results: Neoplaslc cells m MEC (high grade) were recognized by UEA where it was observed a weak cytoplasmic