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P233 Lichenoid dysplasia and malignant transformation of oral lichen planus and oral lichenoid lesions
Poster abstracts, Saturday 19 May
P232 Tumour thickness of epidermoid carcinoma of floor of mouth and mobile tongue: a retrospective study of 124 cases M. El Okeily1 *, M. El Bouihi1 , A.S. Ricard1 , C. MajoufreLefebvre1 , C. Deminiere1 , F. Siberchicot1 , N. Zwetyenga1 . 1 Universit´ e Victor S´ egalen, France Introduction: Treatment of cancer of floor of mouth and oral tongue is essentially based on TNM staging system. However this classification does not include the tumour thickness in T1T3, but it is known that solid tumours have a three-dimensional growth. The aim of our retrospective study was to confirm the prognostic value of thickness in oral squamous cell carcinoma. Patients and Methods: There were 124 patients. The mean age was 59.3 years. Several data have been analyzed including tumour thickness and other prognosis factors. Results: The 2 and 5-year probabilities of global survival were respectively 85.6% and 77.3%. The width of the tumor, cervical node invasion and the tumor thickness were prognosis factors. The median of tumor thickness for our series was 6.5 mm. Cervical node invasion were statically linked to the importance of the tumor thickness. Multimodal analysis showed 2 independents factors: cervical node invasion and a tumor classified T3. Discussion: With an acceptable mean follow-up, this homogenous series confirmed that the prognosis is worse when the tumor thickness is greater than 6.5 mm. Tumour thickness needs to be integrated in TNM classification for T1, T2 and T3. Keywords: epidermoid carcinoma, thickness, oral cavity P233 Lichenoid dysplasia and malignant transformation of oral lichen planus and oral lichenoid lesions: a clinical evaluation of 20 patients P. Vescovi, M. Meleti *, M. Manfredi, E. Merigo, R. Guidotti, A. Ripasarti. University of Parma, Italy Some controversy surrounds the question of whether oral lichen planus (OLP) is a potentially malignant condition. It has been reported in the literature that the malignant transformation rate of OLP may range from 0.4 to 4.7% but no accordance exists on these data. Furthermore, clinicians disagree about the clinical diagnosis of OLP. More restrictive histological criteria have been introduced to help distinguish between oral epithelial dysplasia (OED), OLP and “oral lichenoid lesion” (OLL). Final diagnosis of OLP requires fulfilment of both clinical and histopathological parameters. The term OLL could be used when the condition is histopathologically typical of OLP but clinically only “compatible” with OLP, or vice versa. The results of several studies seem to support the concept of an increased risk for development of oral squamous cell carcinoma (SCC) in patients with OLP. Such a risk appears to be higher in patient ts with OLL. This study reports our clinical experience with 20 patients affected by OLP or OLL referred to the Division of Oral Pathology and Medicine of University of Parma between 1990 and 2006 who developed oral SCC. The majority of patients were female (16/20, 80%) and the tongue, as well as the floor of the mouth, were the sites mostly affected by malignant lesions.
Three patients (15%) developed recurrences of oral SCC during follow-up. The possible association of OLP/OLL and oral SCC, and the association of OLP, oral SCC and hepatitis C virus (HCV) were evaluated retrospectively. Eight out of 20 with oral SCC (2.6%) had been previously clinically and histopathologically evaluated with a diagnosis of OLP and 12 (4%) with OLL. Of these 20, 9 patients (45%) were also positive for the antiHCV antibody. Our results give support to the hypothesis of a possible premalignant nature of OLL and to the different premalignant potential of OLL and OLP. Keywords: oral squamous cell carcinoma, oral lichen planus, oral lichenoid lesions, malignant transformation P234 Adam15 gene expression and correlation with 1q21 amplification in head and neck squamous cell carcinomas E. Ambrosio1 , S. Silveira1 , C. Silveira1 , F. Soares2 , L. Kowalski2 , S. Rogatto1 *. 1 S˜ ao Paulo State University, Brazil, 2 A.C. Camargo Hospital, Brazil The progression of solid tumors to metastatic diseases includes essential steps that support the detachment of cells from the surrounding extracellular matrix (ECM), tumor cell survival, and tissue invasion. One family of proteins supporting malignant progression is zinc-binding matrix metalloproteinmetalloproteinases (MMPs). The role of MMPs in metastatic progression has been extensively studied in tumor biology. An intriguing family of membrane metalloproteinases has been identified in the extracellular processing of these cell surface molecules, the ADAM family (A Disintegrin that contains A Metalloproteinase). ADAM15, located at 1q21.3, is known to be amplified in many cancers, including metastatic prostate cancer, breast cancer, and melanoma. In the present study, we used two probes flanking 1q21.3 by dual color FISH and ADAM15 expression by real time reverse transcription PCR (qRT-PCR) in head and neck squamous cell carcinomas. We analyzed two probes mapped at 1q21, RP11 180B22 (2.56 Mb upstream ADAM15 gene) and RP11 205M9 (0.78 Mb downstream to ADAM15 gene) in 28 cases (18 oral cavity, 4 laryngeal and 6 pharyngeal tumors). The mean copy number varied from 4 to >10 in 12 and 11 cases, respectively. Homogeneously staining region was detected only in oral cavity tumors (three and one, respectively). Three of them died of disease in a period of 11 20 months. In a second group of samples (14 oral cavity and 14 laryngeal tumors) matched normal adjacent tissue, ADAM15 expression was evaluated by qRT-PCR. We observed an over-expression of ADAM15 in 2/14 oral cavity tumors and 12/14 laryngeal carcinomas. Four patients with laryngeal carcinomas presented metastasis and one with oral cavity carcinoma died of disease in 8 months. These results provide new data and suggested that ADAM15 may play an important role in the progression to metastatic disease, in both, oral and laryngeal squamous cell carcinomas. Keywords: ADAM15, FISH, qRT-PCR, head and neck cancer P235 PHF21B as a putative tumor supressor gene associated with familial history in HNSCC F. Bertonha1 , P. dos Reis2 , C. Rainho1 , S. Kamel-Reid2 , L. Kowalski3 , S. Rogatto1 *. 1 S˜ ao Paulo State University, Brazil, 2 Princess Margaret Hospital University Health Network, Canada, 3 A.C. Camargo Cancer Hospital, Brazil Head and neck squamous cells carcinoma (HNSCC) is the fifth most incident tumor in the Brazilian population. Until recently little attention has been paid to hereditary factors involved in its etiology. Initial studies revealed that oral cancer tends to aggregate in families. Additional evidences were provided by the occurrence of multiple primary tumors in HNSCC patients, by the association of familial history with an early age of onset of the disease, and by family members without habits such
Poster abstracts
three grades of tumors along with their cut margins, although their intensity was much higher in poorly differentiated SCC. Fifteen samples each of all the three grades of tumors along with their cut margins were analyzed. Correlation between clinical parameters and the expression of CK, Vimentin, a6b4 will be evaluated statistically. Thus in conclusion our study indicates that CK has potential to be used as surrogate markers for the diagnosis and prognosis of oral cancer patients. Keywords: Cytokeratin, Buccal Mucosa, Vimentin, Precancer
201
P232 Tumour thickness of epidermoid carcinoma of floor of mouth and mobile tongue: a retrospective study of 124 cases M. El Okeily1 *, M. El Bouihi1 , A.S. Ricard1 , C. MajoufreLefebvre1 , C. Deminiere1 , F. Siberchicot1 , N. Zwetyenga1 . 1 Universit´ e Victor S´ egalen, France Introduction: Treatment of cancer of floor of mouth and oral tongue is essentially based on TNM staging system. However this classification does not include the tumour thickness in T1T3, but it is known that solid tumours have a three-dimensional growth. The aim of our retrospective study was to confirm the prognostic value of thickness in oral squamous cell carcinoma. Patients and Methods: There were 124 patients. The mean age was 59.3 years. Several data have been analyzed including tumour thickness and other prognosis factors. Results: The 2 and 5-year probabilities of global survival were respectively 85.6% and 77.3%. The width of the tumor, cervical node invasion and the tumor thickness were prognosis factors. The median of tumor thickness for our series was 6.5 mm. Cervical node invasion were statically linked to the importance of the tumor thickness. Multimodal analysis showed 2 independents factors: cervical node invasion and a tumor classified T3. Discussion: With an acceptable mean follow-up, this homogenous series confirmed that the prognosis is worse when the tumor thickness is greater than 6.5 mm. Tumour thickness needs to be integrated in TNM classification for T1, T2 and T3. Keywords: epidermoid carcinoma, thickness, oral cavity P233 Lichenoid dysplasia and malignant transformation of oral lichen planus and oral lichenoid lesions: a clinical evaluation of 20 patients P. Vescovi, M. Meleti *, M. Manfredi, E. Merigo, R. Guidotti, A. Ripasarti. University of Parma, Italy Some controversy surrounds the question of whether oral lichen planus (OLP) is a potentially malignant condition. It has been reported in the literature that the malignant transformation rate of OLP may range from 0.4 to 4.7% but no accordance exists on these data. Furthermore, clinicians disagree about the clinical diagnosis of OLP. More restrictive histological criteria have been introduced to help distinguish between oral epithelial dysplasia (OED), OLP and “oral lichenoid lesion” (OLL). Final diagnosis of OLP requires fulfilment of both clinical and histopathological parameters. The term OLL could be used when the condition is histopathologically typical of OLP but clinically only “compatible” with OLP, or vice versa. The results of several studies seem to support the concept of an increased risk for development of oral squamous cell carcinoma (SCC) in patients with OLP. Such a risk appears to be higher in patient ts with OLL. This study reports our clinical experience with 20 patients affected by OLP or OLL referred to the Division of Oral Pathology and Medicine of University of Parma between 1990 and 2006 who developed oral SCC. The majority of patients were female (16/20, 80%) and the tongue, as well as the floor of the mouth, were the sites mostly affected by malignant lesions.
Three patients (15%) developed recurrences of oral SCC during follow-up. The possible association of OLP/OLL and oral SCC, and the association of OLP, oral SCC and hepatitis C virus (HCV) were evaluated retrospectively. Eight out of 20 with oral SCC (2.6%) had been previously clinically and histopathologically evaluated with a diagnosis of OLP and 12 (4%) with OLL. Of these 20, 9 patients (45%) were also positive for the antiHCV antibody. Our results give support to the hypothesis of a possible premalignant nature of OLL and to the different premalignant potential of OLL and OLP. Keywords: oral squamous cell carcinoma, oral lichen planus, oral lichenoid lesions, malignant transformation P234 Adam15 gene expression and correlation with 1q21 amplification in head and neck squamous cell carcinomas E. Ambrosio1 , S. Silveira1 , C. Silveira1 , F. Soares2 , L. Kowalski2 , S. Rogatto1 *. 1 S˜ ao Paulo State University, Brazil, 2 A.C. Camargo Hospital, Brazil The progression of solid tumors to metastatic diseases includes essential steps that support the detachment of cells from the surrounding extracellular matrix (ECM), tumor cell survival, and tissue invasion. One family of proteins supporting malignant progression is zinc-binding matrix metalloproteinmetalloproteinases (MMPs). The role of MMPs in metastatic progression has been extensively studied in tumor biology. An intriguing family of membrane metalloproteinases has been identified in the extracellular processing of these cell surface molecules, the ADAM family (A Disintegrin that contains A Metalloproteinase). ADAM15, located at 1q21.3, is known to be amplified in many cancers, including metastatic prostate cancer, breast cancer, and melanoma. In the present study, we used two probes flanking 1q21.3 by dual color FISH and ADAM15 expression by real time reverse transcription PCR (qRT-PCR) in head and neck squamous cell carcinomas. We analyzed two probes mapped at 1q21, RP11 180B22 (2.56 Mb upstream ADAM15 gene) and RP11 205M9 (0.78 Mb downstream to ADAM15 gene) in 28 cases (18 oral cavity, 4 laryngeal and 6 pharyngeal tumors). The mean copy number varied from 4 to >10 in 12 and 11 cases, respectively. Homogeneously staining region was detected only in oral cavity tumors (three and one, respectively). Three of them died of disease in a period of 11 20 months. In a second group of samples (14 oral cavity and 14 laryngeal tumors) matched normal adjacent tissue, ADAM15 expression was evaluated by qRT-PCR. We observed an over-expression of ADAM15 in 2/14 oral cavity tumors and 12/14 laryngeal carcinomas. Four patients with laryngeal carcinomas presented metastasis and one with oral cavity carcinoma died of disease in 8 months. These results provide new data and suggested that ADAM15 may play an important role in the progression to metastatic disease, in both, oral and laryngeal squamous cell carcinomas. Keywords: ADAM15, FISH, qRT-PCR, head and neck cancer P235 PHF21B as a putative tumor supressor gene associated with familial history in HNSCC F. Bertonha1 , P. dos Reis2 , C. Rainho1 , S. Kamel-Reid2 , L. Kowalski3 , S. Rogatto1 *. 1 S˜ ao Paulo State University, Brazil, 2 Princess Margaret Hospital University Health Network, Canada, 3 A.C. Camargo Cancer Hospital, Brazil Head and neck squamous cells carcinoma (HNSCC) is the fifth most incident tumor in the Brazilian population. Until recently little attention has been paid to hereditary factors involved in its etiology. Initial studies revealed that oral cancer tends to aggregate in families. Additional evidences were provided by the occurrence of multiple primary tumors in HNSCC patients, by the association of familial history with an early age of onset of the disease, and by family members without habits such
Poster abstracts
three grades of tumors along with their cut margins, although their intensity was much higher in poorly differentiated SCC. Fifteen samples each of all the three grades of tumors along with their cut margins were analyzed. Correlation between clinical parameters and the expression of CK, Vimentin, a6b4 will be evaluated statistically. Thus in conclusion our study indicates that CK has potential to be used as surrogate markers for the diagnosis and prognosis of oral cancer patients. Keywords: Cytokeratin, Buccal Mucosa, Vimentin, Precancer
201