- Email: [email protected]
PAMAM Megamer (G2-G2) as a versatile tool in gene delivery
Abstracts
Poster – [A-10-382-1] Investigation of Thalassmia in Khorasan province Maryam Taghavia, Fatemeh Bayata, Morteza Zadeh Marjana, Setareh Ahmadia, Alireza Kordafsharia, Morteza Karimipoura, Sirous Zeinalib a Molecular Medicine Dept., Pasteur Institute of Iran, Iran b Department of Molecular Medicine, Pasteur Institute of Iran, Iran E-mail addresses: [email protected] (M. Taghavi), [email protected] (F. Bayat), [email protected] (M. Zadeh Marjan), [email protected] (S. Ahmadi), [email protected] (A. Kordafshari), [email protected] (M. Karimipour), [email protected] (S. Zeinali)
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with FRDA are compound heterozygotes for a GAA expansion in the disease-causing range in one FXN allele and another inactivating FXN mutation in the other allele. Aim of present study was to investigate exon 1 in FRDA gene in patients with clinically symptomatic Friedreich ataxia that do not have GAA triplet-repeat expansion in intron 1 of FXN. Materials and methods: We analyzed exon 1in 7 patients suspected with FA using PCR and sequencing. Results: Only one person carried G815324T heterozygote mutation in exon 1 and an A to G in exon 1 nt:815284 in our patients. Discusion: We believed in this report because of consanguinity marriage in Iran some patients with homozygote mutation may show FA phenotype. Keywords: Friedreich ataxia, Exon 1, Mutation
Introduction: Thalassemia is one of the common genetic disease worldwide and it is highly prevalent among people of Mediterranean, African or Asian descent. The aim of this study was molecular characterization of α- and β-globin genes in ambiguous carriers of Khorasan province referred from primary care center (PHC) to Pasteur Institute of Iran. Materials and method: Genomic DNA was extracted using the salting out method. ARMS- PCR and RFLP was used for molecular characterization of β-globin gene cluster and multiplex Gap PCR was exploited for detection of α-globin gene deletions. DNA sequencing for point mutations in α and β-globin gene was done for appropriate cases. Results: Of total of 51 cases, Based on hematological parameter 24 were β-thal carrier, 27 α-thal and Hb variant. Molecular analysis in β-thal carrier group showed IVSII-I is the most common mutation(33%),followed by CD41/42 (16%),Fr8/9 and IVSI-5(12%),CD44 and -25del(8%) ,CD16 and Fr81/82(4%) and 1.9% were Hb D. In α-thalassemia group the causative mutation was found in 14 individual –α3.7 /αα was the most common deletion and -5nt(4%),–α4.2 /αα , -20.5/ αα were the other mutations Prenatal diagnosis was performed for 9 families,11% of fetuses were normal, 22% fetuses had β-thalassemia major, and 56% was minor. Conclusion: Since the Iranian population is a mixture of different groups, it's necessary to determine the frequency and distribution of mutations in α- and β-globin in the different part of country. The results presented here can be used as a basis of prenatal diagnosis in Khorasan province. Keywords: β-thalassemia, α-thalassemia, Khorasan province doi:10.1016/j.clinbiochem.2011.08.697
Poster – [A-10-398-1] Investigation of exon 1 in FRDA gene in patients with clinically symptomatic Friedreich ataxia Naseroleslami Maryama, Parivar Kazema, Sangarian Saraa, Houshmand Massoudb a Science and Research Branch, Islamic Azad University, Tehran, Iran b Institute of Genetic Engineering and Biotechnology, Iran E-mail addresses: [email protected] (N. Maryam), [email protected] (P. Kazem), [email protected] (S. Sara), [email protected] (H. Massoud) Introduction: Friedreich ataxia (FA) is an autosomal recessive disorder that is typically associated with dysarthria, muscle weakness, spasticity in the lower limbs, scoliosis, bladder dysfunction, absent lower limb reflexes, and loss of position and vibration sense. Approximately two-thirds of this patient have cardiomyopathy; up to 30% have diabetes mellitus. Individuals with FRDA have identifiable mutations in the FXN gene. The most common type of mutation, which is observed on both alleles in more than 98% of individuals with FRDA, is a GAA tripletrepeat expansion in intron 1 of FXN. Approximately 2% of individuals
doi:10.1016/j.clinbiochem.2011.08.698
Poster – [A-10-399-1] GSTT1 and GSTM1 gene polymorphisms in patients with systemic lupus erythematosus (SLE) Jafari Mehdi, Salimi Saideh, Nakhaee Alireza, Zakeri Zahra, Saavani Mohsen Zahedan University of Medical Sciences, Zahedan, Iran E-mail addresses: [email protected] (J. Mehdi), [email protected] (S. Saideh), [email protected] (N. Alireza), [email protected] (Z. Zahra), [email protected] (S. Mohsen) Introduction: Oxidative stress caused by poor detoxification efficiency of reactive oxygen species (ROS) may play a role in the development of systemic lupus erythematosus (SLE). Glutathione S-transferase (GST) is involved in the detoxification of ROS and genetic polymorphisms of GSTM1 and GSTT1 are associated with altered enzyme activity. The aim of this study was to determine whether GSTM1 (deletion) and GSTT1 (deletion) polymorphisms are associated with susceptibility to SLE. Methods: In this case–control study which was conducted in the city of Zahedan, we investigated the polymorphisms of GSTM1 and GSTT1 in 80 patients with systemic lupus erythematosus and 118 as the control group, using the technique multiplex PCR method. Conclusion: The result showed that the overall OR was 1.9 (95% CI = 1–3.6) for GSTT1 polymorphism, while for GSTM1 polymorphism, the overall OR was 1.4 (95% CI = 0.84–2.3). Result: The results suggested that GSTT1 deletion as a risk factor for SLE and GSTM1 gene polymorphism might not be not associated with increased risk of SLE. Keywords: Systemic lupus erythematosus, GSTT1, GSTM1, Polymorphisms doi:10.1016/j.clinbiochem.2011.08.699
E.Poster – [A-10-415-1] PAMAM Megamer (G2-G2) as a versatile tool in gene delivery Mashhad Hoda Atapour a, Majid Mojaradb, Reza Raoofianb, Fatemeh Baghebanib, Omid Louiea, Abdoulhossien Massoudia, Mohamad Soukhtanlooc, Vahedi Hooshanga a Department of Chemistry, Payame Noor University (PNU), Mashhad, Iran b Department of Medical Genetics, Faculty of Medicine, Mashhad University Of Medical Sciences, Mashhad, Iran c Department Of Biochemistry, Faculty of Medicine, Mashhad University Of Medical Sciences, Mashhad, Iran
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Abstracts
E-mail addresses: [email protected] (M.H. Atapour), [email protected] (M. Mojarad), [email protected] (R. Raoofian), [email protected] (F. Baghebani), [email protected] (O. Louie), [email protected] (A. Massoudi), [email protected] (M. Soukhtanloo), [email protected] (V. Hooshang) Introduction: Dendrimers represent one of several non-viral systems used for delivering nucleic acids into cells. They are synthetic hyperbranched polymers which are highly soluble in aqueous solutions. Dendrimers with positively charged terminal groups can bind DNA forming complexes, termed dendriplexes and trapped oligonucleotides are protected from nucleases. However, they are less efficient than viral vectors. These macromolecules have potential for use in gene therapy and other therapeutic applications due to their safety and lack of immunogenicity. Method: In an attempt to discover and develop novel potential gene delivery analog, we have synthesized PAMAM Megamer (G2G2) by using 2 kinds of dendrimers, PAMAM G2 and PAMAM-COOH G2 for identification of new gene delivery agent. In this study, we have performed an in-vitro evaluation of cytotoxic potential of the newly synthesized PAMAM Megamer (G2-G2), on human breast cancer cell line (MCF-7) by using MTT assay. Results: Results confirmed no significant cytotoxicity of PAMAM Megamer (G2-G2) (7.5–500 μM) against MCF-7 cells. For evaluation the protectivity of these molecules we have carried out comparative DNase I protection assay with different concentrations of PAMAM Megamer and Plasmid. Their ability to deliver oligonucleotides to mammalian cells was assessed by measurement of GFP fluorescence following introduction of pEGFP-N1-megmer complex into MCF-7 and HEK293 cell lines. Results confirmed no significant cytotoxicity of PAMAM Megamer (G2-G2) (7.5– 500 μM) against MCF-7 cells. Ethidium-bromide stained Agarose gels showed PAMAM Megamer ability to protect plasmid from DNase I in a dose dependent manner. Measurement of GFP fluorescence in MCF-7 and HEK293 cell lines using VICTOR X5 multilabel reader, confirmed these complex ability to introduction of DNA into cells. Conclusion: Results suggested PAMAM Megamer (G2-G2) as a new tool for gene delivery. Keywords: Megamer, Gene delivery, MCF-7 cell line, HEK293 cell line doi:10.1016/j.clinbiochem.2011.08.700
Poster – [A-10-483-4] Study of the interaction between Ropinirole hydrochloride and human serum albumin as binary system by three-dimensional fluorescence spectroscopy Mahaki Hanieha, Chamani Jamshidb, Saberi Mohamad Rezaa, Vahidzadeh Masomeha a Department of Biology, Faculty of Sciences, Islamic Azad University, Mashhad Branch, Mashhad, Iran b Medicinal Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (M. Hanieh), [email protected] (C. Jamshid), [email protected] (S.M. Reza) [email protected] (M. Hanieh) Intruduction: Human serum albumin is the most abundant protein in plasma. Its three-dimensional structure has been determined through X-ray crystallographic measurements. Ropinirole is a selective agonist of dopamine D2/3 receptors and its efficacy in the treatment of Parkinson's disease (PD) has been established.
Materials and method: Three-dimensional fluorescence spectra were recorded under the following conditions: The emission wavelength was recorded between 300 and 600 nm, the initial excitation wavelength was set to 200 nm with increment of 10 nm. Results and discussion: Peak a is the Rayleigh scattering peak (=) and peak b is the second-ordered scattering peak (= 2). The fluorescence intensity of peak a and peak b increased with the addition of RP. The possible reason is that a RP–HSA complex came into being after the addition of RP, increasing the diameter of the macromolecule which in turn resulted in an enhanced scattering effect. Peak 1 mainly reveals the spectral behavior of Trp and Tyr residues. Results: The reason is that when HSA is excited at 280 nm, it mainly reveals the intrinsic fluorescence of Trp and Tyr residues. Beside peak 1, there is another fluorescence peak 2 (=230.0 nm, =340.0 nm) that mainly reflects the fluorescence spectral behavior of the polypeptide backbone structure of HAS. The fluorescence intensity of peak 2 decreased after the addition of RP, which means that the peptide strands structure of HSA has been changed. Keywords: Ropinirole hydrochloride, Human serum albumin, Three dimensional doi:10.1016/j.clinbiochem.2011.08.701
Poster – [A-10-493-2] Association between cholesteryl ester transfer protein taqIB variants and risk of coronary artery disease in population of west of Iran Rahimi Zohreha, Nourozi-Rad Rezaa, Vaisi-Raygani Asada, Saidi Mohammad-Rezab, Rahimi Zibac, Parsian Abbasd a Department of Biochemistry, Medical School, Daneshgah Avenue, Kermanshah, Iran b Department of Cardiology, Medical School, Daneshgah Avenue, Kermanshah, Iran c Medical Biology Research Center, Daneshgah Avenue, Kermanshah, Iran d Division of Neuroscience Behavior, NIAAA, National Institutes of Health, Rockville, Maryland, USA E-mail address: [email protected] (R. Zohreh) Introduction: There are inconsistent results about the association of cholesteryl ester transfer protein TaqIB (CETP TaqIB) variants, HDLC levels and the risk of coronary artery disease (CAD). Methods: To determine the frequency of CETP TaqIB variants and to examine the possible association between CETP TaqIB polymorphism with CAD we studied 207 unrelated CAD patients and 92 controls. The CETP TaqIB variants were detected by PCR-RFLP. Results: Logistic regression analysis indicated that the B1 allele of CETP was significantly associated with increased risk of CAD [OR 1.65 (95% CI 1.2–2.3, p = 0.005)]. Adjusted logistic regression analysis for the effects of age, sex, hypertension, diabetes and hyperlipidemia was performed and a significant association was found between the B1 allele and risk of CAD with [OR 1.9 (95% CI 1–3.6, p = 0.049)] in CAD patients. There were no associations between the CETP alleles and the levels of triglycerides, total cholesterol, LDL-C and HDL-C in studied groups. Discussion: The results of present study revealed that CETP B1 allele is associated with increased risk of CAD independent of plasma HDL-C level in our population. Keywords: CETP TaqIB, Coronary artery disease, HDL-C doi:10.1016/j.clinbiochem.2011.08.702
Poster – [A-10-382-1] Investigation of Thalassmia in Khorasan province Maryam Taghavia, Fatemeh Bayata, Morteza Zadeh Marjana, Setareh Ahmadia, Alireza Kordafsharia, Morteza Karimipoura, Sirous Zeinalib a Molecular Medicine Dept., Pasteur Institute of Iran, Iran b Department of Molecular Medicine, Pasteur Institute of Iran, Iran E-mail addresses: [email protected] (M. Taghavi), [email protected] (F. Bayat), [email protected] (M. Zadeh Marjan), [email protected] (S. Ahmadi), [email protected] (A. Kordafshari), [email protected] (M. Karimipour), [email protected] (S. Zeinali)
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with FRDA are compound heterozygotes for a GAA expansion in the disease-causing range in one FXN allele and another inactivating FXN mutation in the other allele. Aim of present study was to investigate exon 1 in FRDA gene in patients with clinically symptomatic Friedreich ataxia that do not have GAA triplet-repeat expansion in intron 1 of FXN. Materials and methods: We analyzed exon 1in 7 patients suspected with FA using PCR and sequencing. Results: Only one person carried G815324T heterozygote mutation in exon 1 and an A to G in exon 1 nt:815284 in our patients. Discusion: We believed in this report because of consanguinity marriage in Iran some patients with homozygote mutation may show FA phenotype. Keywords: Friedreich ataxia, Exon 1, Mutation
Introduction: Thalassemia is one of the common genetic disease worldwide and it is highly prevalent among people of Mediterranean, African or Asian descent. The aim of this study was molecular characterization of α- and β-globin genes in ambiguous carriers of Khorasan province referred from primary care center (PHC) to Pasteur Institute of Iran. Materials and method: Genomic DNA was extracted using the salting out method. ARMS- PCR and RFLP was used for molecular characterization of β-globin gene cluster and multiplex Gap PCR was exploited for detection of α-globin gene deletions. DNA sequencing for point mutations in α and β-globin gene was done for appropriate cases. Results: Of total of 51 cases, Based on hematological parameter 24 were β-thal carrier, 27 α-thal and Hb variant. Molecular analysis in β-thal carrier group showed IVSII-I is the most common mutation(33%),followed by CD41/42 (16%),Fr8/9 and IVSI-5(12%),CD44 and -25del(8%) ,CD16 and Fr81/82(4%) and 1.9% were Hb D. In α-thalassemia group the causative mutation was found in 14 individual –α3.7 /αα was the most common deletion and -5nt(4%),–α4.2 /αα , -20.5/ αα were the other mutations Prenatal diagnosis was performed for 9 families,11% of fetuses were normal, 22% fetuses had β-thalassemia major, and 56% was minor. Conclusion: Since the Iranian population is a mixture of different groups, it's necessary to determine the frequency and distribution of mutations in α- and β-globin in the different part of country. The results presented here can be used as a basis of prenatal diagnosis in Khorasan province. Keywords: β-thalassemia, α-thalassemia, Khorasan province doi:10.1016/j.clinbiochem.2011.08.697
Poster – [A-10-398-1] Investigation of exon 1 in FRDA gene in patients with clinically symptomatic Friedreich ataxia Naseroleslami Maryama, Parivar Kazema, Sangarian Saraa, Houshmand Massoudb a Science and Research Branch, Islamic Azad University, Tehran, Iran b Institute of Genetic Engineering and Biotechnology, Iran E-mail addresses: [email protected] (N. Maryam), [email protected] (P. Kazem), [email protected] (S. Sara), [email protected] (H. Massoud) Introduction: Friedreich ataxia (FA) is an autosomal recessive disorder that is typically associated with dysarthria, muscle weakness, spasticity in the lower limbs, scoliosis, bladder dysfunction, absent lower limb reflexes, and loss of position and vibration sense. Approximately two-thirds of this patient have cardiomyopathy; up to 30% have diabetes mellitus. Individuals with FRDA have identifiable mutations in the FXN gene. The most common type of mutation, which is observed on both alleles in more than 98% of individuals with FRDA, is a GAA tripletrepeat expansion in intron 1 of FXN. Approximately 2% of individuals
doi:10.1016/j.clinbiochem.2011.08.698
Poster – [A-10-399-1] GSTT1 and GSTM1 gene polymorphisms in patients with systemic lupus erythematosus (SLE) Jafari Mehdi, Salimi Saideh, Nakhaee Alireza, Zakeri Zahra, Saavani Mohsen Zahedan University of Medical Sciences, Zahedan, Iran E-mail addresses: [email protected] (J. Mehdi), [email protected] (S. Saideh), [email protected] (N. Alireza), [email protected] (Z. Zahra), [email protected] (S. Mohsen) Introduction: Oxidative stress caused by poor detoxification efficiency of reactive oxygen species (ROS) may play a role in the development of systemic lupus erythematosus (SLE). Glutathione S-transferase (GST) is involved in the detoxification of ROS and genetic polymorphisms of GSTM1 and GSTT1 are associated with altered enzyme activity. The aim of this study was to determine whether GSTM1 (deletion) and GSTT1 (deletion) polymorphisms are associated with susceptibility to SLE. Methods: In this case–control study which was conducted in the city of Zahedan, we investigated the polymorphisms of GSTM1 and GSTT1 in 80 patients with systemic lupus erythematosus and 118 as the control group, using the technique multiplex PCR method. Conclusion: The result showed that the overall OR was 1.9 (95% CI = 1–3.6) for GSTT1 polymorphism, while for GSTM1 polymorphism, the overall OR was 1.4 (95% CI = 0.84–2.3). Result: The results suggested that GSTT1 deletion as a risk factor for SLE and GSTM1 gene polymorphism might not be not associated with increased risk of SLE. Keywords: Systemic lupus erythematosus, GSTT1, GSTM1, Polymorphisms doi:10.1016/j.clinbiochem.2011.08.699
E.Poster – [A-10-415-1] PAMAM Megamer (G2-G2) as a versatile tool in gene delivery Mashhad Hoda Atapour a, Majid Mojaradb, Reza Raoofianb, Fatemeh Baghebanib, Omid Louiea, Abdoulhossien Massoudia, Mohamad Soukhtanlooc, Vahedi Hooshanga a Department of Chemistry, Payame Noor University (PNU), Mashhad, Iran b Department of Medical Genetics, Faculty of Medicine, Mashhad University Of Medical Sciences, Mashhad, Iran c Department Of Biochemistry, Faculty of Medicine, Mashhad University Of Medical Sciences, Mashhad, Iran
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Abstracts
E-mail addresses: [email protected] (M.H. Atapour), [email protected] (M. Mojarad), [email protected] (R. Raoofian), [email protected] (F. Baghebani), [email protected] (O. Louie), [email protected] (A. Massoudi), [email protected] (M. Soukhtanloo), [email protected] (V. Hooshang) Introduction: Dendrimers represent one of several non-viral systems used for delivering nucleic acids into cells. They are synthetic hyperbranched polymers which are highly soluble in aqueous solutions. Dendrimers with positively charged terminal groups can bind DNA forming complexes, termed dendriplexes and trapped oligonucleotides are protected from nucleases. However, they are less efficient than viral vectors. These macromolecules have potential for use in gene therapy and other therapeutic applications due to their safety and lack of immunogenicity. Method: In an attempt to discover and develop novel potential gene delivery analog, we have synthesized PAMAM Megamer (G2G2) by using 2 kinds of dendrimers, PAMAM G2 and PAMAM-COOH G2 for identification of new gene delivery agent. In this study, we have performed an in-vitro evaluation of cytotoxic potential of the newly synthesized PAMAM Megamer (G2-G2), on human breast cancer cell line (MCF-7) by using MTT assay. Results: Results confirmed no significant cytotoxicity of PAMAM Megamer (G2-G2) (7.5–500 μM) against MCF-7 cells. For evaluation the protectivity of these molecules we have carried out comparative DNase I protection assay with different concentrations of PAMAM Megamer and Plasmid. Their ability to deliver oligonucleotides to mammalian cells was assessed by measurement of GFP fluorescence following introduction of pEGFP-N1-megmer complex into MCF-7 and HEK293 cell lines. Results confirmed no significant cytotoxicity of PAMAM Megamer (G2-G2) (7.5– 500 μM) against MCF-7 cells. Ethidium-bromide stained Agarose gels showed PAMAM Megamer ability to protect plasmid from DNase I in a dose dependent manner. Measurement of GFP fluorescence in MCF-7 and HEK293 cell lines using VICTOR X5 multilabel reader, confirmed these complex ability to introduction of DNA into cells. Conclusion: Results suggested PAMAM Megamer (G2-G2) as a new tool for gene delivery. Keywords: Megamer, Gene delivery, MCF-7 cell line, HEK293 cell line doi:10.1016/j.clinbiochem.2011.08.700
Poster – [A-10-483-4] Study of the interaction between Ropinirole hydrochloride and human serum albumin as binary system by three-dimensional fluorescence spectroscopy Mahaki Hanieha, Chamani Jamshidb, Saberi Mohamad Rezaa, Vahidzadeh Masomeha a Department of Biology, Faculty of Sciences, Islamic Azad University, Mashhad Branch, Mashhad, Iran b Medicinal Chemistry Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (M. Hanieh), [email protected] (C. Jamshid), [email protected] (S.M. Reza) [email protected] (M. Hanieh) Intruduction: Human serum albumin is the most abundant protein in plasma. Its three-dimensional structure has been determined through X-ray crystallographic measurements. Ropinirole is a selective agonist of dopamine D2/3 receptors and its efficacy in the treatment of Parkinson's disease (PD) has been established.
Materials and method: Three-dimensional fluorescence spectra were recorded under the following conditions: The emission wavelength was recorded between 300 and 600 nm, the initial excitation wavelength was set to 200 nm with increment of 10 nm. Results and discussion: Peak a is the Rayleigh scattering peak (=) and peak b is the second-ordered scattering peak (= 2). The fluorescence intensity of peak a and peak b increased with the addition of RP. The possible reason is that a RP–HSA complex came into being after the addition of RP, increasing the diameter of the macromolecule which in turn resulted in an enhanced scattering effect. Peak 1 mainly reveals the spectral behavior of Trp and Tyr residues. Results: The reason is that when HSA is excited at 280 nm, it mainly reveals the intrinsic fluorescence of Trp and Tyr residues. Beside peak 1, there is another fluorescence peak 2 (=230.0 nm, =340.0 nm) that mainly reflects the fluorescence spectral behavior of the polypeptide backbone structure of HAS. The fluorescence intensity of peak 2 decreased after the addition of RP, which means that the peptide strands structure of HSA has been changed. Keywords: Ropinirole hydrochloride, Human serum albumin, Three dimensional doi:10.1016/j.clinbiochem.2011.08.701
Poster – [A-10-493-2] Association between cholesteryl ester transfer protein taqIB variants and risk of coronary artery disease in population of west of Iran Rahimi Zohreha, Nourozi-Rad Rezaa, Vaisi-Raygani Asada, Saidi Mohammad-Rezab, Rahimi Zibac, Parsian Abbasd a Department of Biochemistry, Medical School, Daneshgah Avenue, Kermanshah, Iran b Department of Cardiology, Medical School, Daneshgah Avenue, Kermanshah, Iran c Medical Biology Research Center, Daneshgah Avenue, Kermanshah, Iran d Division of Neuroscience Behavior, NIAAA, National Institutes of Health, Rockville, Maryland, USA E-mail address: [email protected] (R. Zohreh) Introduction: There are inconsistent results about the association of cholesteryl ester transfer protein TaqIB (CETP TaqIB) variants, HDLC levels and the risk of coronary artery disease (CAD). Methods: To determine the frequency of CETP TaqIB variants and to examine the possible association between CETP TaqIB polymorphism with CAD we studied 207 unrelated CAD patients and 92 controls. The CETP TaqIB variants were detected by PCR-RFLP. Results: Logistic regression analysis indicated that the B1 allele of CETP was significantly associated with increased risk of CAD [OR 1.65 (95% CI 1.2–2.3, p = 0.005)]. Adjusted logistic regression analysis for the effects of age, sex, hypertension, diabetes and hyperlipidemia was performed and a significant association was found between the B1 allele and risk of CAD with [OR 1.9 (95% CI 1–3.6, p = 0.049)] in CAD patients. There were no associations between the CETP alleles and the levels of triglycerides, total cholesterol, LDL-C and HDL-C in studied groups. Discussion: The results of present study revealed that CETP B1 allele is associated with increased risk of CAD independent of plasma HDL-C level in our population. Keywords: CETP TaqIB, Coronary artery disease, HDL-C doi:10.1016/j.clinbiochem.2011.08.702