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Phagocytosis and flow cytometric analyses: Another steptowards an urgent goal
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The I'elerinau.flmrnu11998, 156, 79-80
Guest Editorial Phagocytosis and How Cytometric Analyses: Another Step Towards an Urgent Goal Flow cytometric m e t h o d s for the analyses o f phagocytosis by neutr<)phils and m ( ) n o c y t e / m a c r o p h a g e s were desc)ibed in the 1980s (Bassoe, 1984, Bass0e el al., 1983). H()wever, the application ()f these techn i q u e s has b e e n slow a n d s t r e n u o u s in t h e v e t e r i n a r y species. Two p a p e r s by Raidal el al (1988a, 1988b), published in this issue of theJ0urhal, describe r e c e n t and valtml:fle m e t h o d o l o g i c a l d e v e l o p m e n t s in tile evaltmtion o f phagocytosis and oxidative I)urst by e q u i n e pe,'ipheral blood neutrophils a n d a l v e o l a r - d e r i v e d m a o ' o p h a g e s . T h e i r ,herbrmation
issue by Raidal el aL (1998a) describes the use o f fluo r e s c e n t l a b e l l e d , but h e a t - k i l l e d b a c t e r i a (an
Aclinobacilhts sui.s-like o r g a n i s m ) as targets for phagocytosis by equine blood neutrophils and alveolar macrophages. This is a step n e a r e r the u r g e n t questitm, but still not the question itselE As the literatttre has progressed through fltLorescent beads, yeast particles and now heat-killed bacteria, I can hardly wait fi)r s o m e o n e to p r o d u c e a p a p e r on the flmctional activity of e q u i n e phagocytes, using tlow cytometry and a live, D,pical equine pathogen, such its .~lr¢7)loco¢'¢us equi or ,~lr¢'plo¢'occll.5 :ooepidemicus. D. HANNANT Cenh'e.fi~r Preventive Medicine Animal Health 7"ru.s+l l.anwades Park Ken!ford Nmvmarket $1{[fol1¢ ('B8 7( rU, U.K. REFERENCES
B.\ssOt.:, C. F.(1984). Processing of Slaphylococcus aureus and zvmosan particles bv human leucocytes measured by flow cytometD'. ((~,lmnetO, 5, 86-91. BAssOv, C. F., LXERt'M,O. D., S~n.lU.:m;,C., O. H.\NH',I.:Rt;,B. (1983). Phagocytosis of bacteria by human leucocytes mcasurcd by flow c\'tomctrv. ProceeR<)SSl, A., NlSlNI, R., l)lzZ<)l.O,.1. (;. & D'AMEt.lO, R. (1989). New, simple, flow cytomeuT techniqtte to discrimimttc I:,etween i,tte,-nalised and melllbrltne-I)<>ttnd pa,ticles in phagocytosis. ([V/tam'by 10, 320-5. F<)I.RSIER, R.J. & ~,vIH.I:,(;. (1990). Phagocylosis of opsonised th,orescent microspheres I)v equine polynlorphonuclear leucocytes..]ottrnal of Velerina O' Medicine B. 37,481-9(I. .]()ll.~xtss()x,A., (;R(-)NI).\III+,G., DEMMERS,S. c~.]ENSEN-X~Y.\ERN, M. (1995). Fh)w cvtomctric studies of the phagocytic capacitics ()f equine neutrophils. Acla ~>lelinaria .O'candinavica 36. 553-562. R.uD.\t., S. 1,., BAIl.i-Y,(;. D. & Li~X'E,D. N.(1998a). The flow c\'lOnlelric evahlation ¢)f phagocytosis by equine F)eripheral bh>od neutrophils and puhnonary alveolar macrophages. The D,terinatyJounlal. 156, 107-116. © 1998 Bailli('reTindall
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TH E VETERINARY .JOU RNAL, 156, 2
RAIDAI., S. L., BAu.ev, G. D. & Low.:, D. N.(1998b). Flow cytometric determination o|" oxidative bursl activity o[ equine peripheral blood and b,'onchoalveolar lavagc derived leucocytes. The |.5,le1~nao,.]mtlvml. 156, 117-1~6
SA..XD, A. M. g: I-l.x¢;H.r,mx, M. (1985). Fh,w cytomelric clr,uactcris'ati(nl o f bovine blood neulrophil phagocylosis o[" [ll.lol'¢?,sccIll bactc'ria and zy,nosan particle's. A¢la 1.5./erinaria Sca,di,avica 2 6 , 2 8 9 - 3 0 7 .
The I'elerinau.flmrnu11998, 156, 79-80
Guest Editorial Phagocytosis and How Cytometric Analyses: Another Step Towards an Urgent Goal Flow cytometric m e t h o d s for the analyses o f phagocytosis by neutr<)phils and m ( ) n o c y t e / m a c r o p h a g e s were desc)ibed in the 1980s (Bassoe, 1984, Bass0e el al., 1983). H()wever, the application ()f these techn i q u e s has b e e n slow a n d s t r e n u o u s in t h e v e t e r i n a r y species. Two p a p e r s by Raidal el al (1988a, 1988b), published in this issue of theJ0urhal, describe r e c e n t and valtml:fle m e t h o d o l o g i c a l d e v e l o p m e n t s in tile evaltmtion o f phagocytosis and oxidative I)urst by e q u i n e pe,'ipheral blood neutrophils a n d a l v e o l a r - d e r i v e d m a o ' o p h a g e s . T h e i r ,herb
issue by Raidal el aL (1998a) describes the use o f fluo r e s c e n t l a b e l l e d , but h e a t - k i l l e d b a c t e r i a (an
Aclinobacilhts sui.s-like o r g a n i s m ) as targets for phagocytosis by equine blood neutrophils and alveolar macrophages. This is a step n e a r e r the u r g e n t questitm, but still not the question itselE As the literatttre has progressed through fltLorescent beads, yeast particles and now heat-killed bacteria, I can hardly wait fi)r s o m e o n e to p r o d u c e a p a p e r on the flmctional activity of e q u i n e phagocytes, using tlow cytometry and a live, D,pical equine pathogen, such its .~lr¢7)loco¢'¢us equi or ,~lr¢'plo¢'occll.5 :ooepidemicus. D. HANNANT Cenh'e.fi~r Preventive Medicine Animal Health 7"ru.s+l l.anwades Park Ken!ford Nmvmarket $1{[fol1¢ ('B8 7( rU, U.K. REFERENCES
B.\ssOt.:, C. F.(1984). Processing of Slaphylococcus aureus and zvmosan particles bv human leucocytes measured by flow cytometD'. ((~,lmnetO, 5, 86-91. BAssOv, C. F., LXERt'M,O. D., S~n.lU.:m;,C., O. H.\NH',I.:Rt;,B. (1983). Phagocytosis of bacteria by human leucocytes mcasurcd by flow c\'tomctrv. Procee
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TH E VETERINARY .JOU RNAL, 156, 2
RAIDAI., S. L., BAu.ev, G. D. & Low.:, D. N.(1998b). Flow cytometric determination o|" oxidative bursl activity o[ equine peripheral blood and b,'onchoalveolar lavagc derived leucocytes. The |.5,le1~nao,.]mtlvml. 156, 117-1~6
SA..XD, A. M. g: I-l.x¢;H.r,mx, M. (1985). Fh,w cytomelric clr,uactcris'ati(nl o f bovine blood neulrophil phagocylosis o[" [ll.lol'¢?,sccIll bactc'ria and zy,nosan particle's. A¢la 1.5./erinaria Sca,di,avica 2 6 , 2 8 9 - 3 0 7 .