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PO20-614 AN OPTIMAL DIETARY APPROACH FOR TREATING POSTPRANDIAL LIPID ABNORMALITIES IN TYPE 2 DIABETIC PATIENTS
Poster Sessions PO20 Nutrition and nutriceuticals in prevention and management of CVD HDL < 40 mg/dL for men, <50 mg/dL for women, and mild hypertension) treated with anthocyanins (3x100mg/d) for 2 months. Before and after 1 and 2 months of treatment the following parameters were determined: serum lipids, glucose, uric acid (by enzymatic method using BioMerieux tests), CRP (immunoturbidimetric method), CAT (method of Bartosz et al.), GSH-Px (method of Rice-Evansa), SOD (method of Misra) activities. Results: It was noticed significantly higher concentrations of lipids (TC, LDL-C, TG), CRP and lower activities of GSH-Px, SOD in patients with MS than in the control group. After two months therapy in comparison with baseline it was observed a significant decrease of: systolic (131.83±12.24 vs 144.20±9.97 mmHg, p<0.001) and diastolic (82.13± 10.33 vs 82.13±10.33 vs 87.20±9.9 mmHg) blood pressure, TC (227.96±33.07 vs 242.80±34.48, p<0.001), LDL-C (146.21±30.93 vs 158.71±35.78, p<0.01), TG (187.58±90 vs 215.92±63.61, p<0.05) and significant increase of SOD (3066.53±542.24 vs 2380.63±419.91, p<0.001), GSH-Px (19.18±9.09 vs 12.60±5.97, p<0.01)activities. Conclusion: Two months therapy with anthocyanins exhibits a hypolipemic and hypotensive efficacy and advantageous effects on the antioxidative enzymes activities in patients with metabolic syndrome. PO20-614
A.A. Rivellese, C. De Natale, L. Patti, L. Bozzetto, P. Massaro, G. Cosatbile, G. Riccardi, G. Annuzzi. Department of Clinical and Experimental Medicine, Federico II University, Naples, Italy To evaluate the effects on postprandial lipaemia of two diets generally recommended for diabetic patients (high-CHO/high-fibre versus low-CHO/high-MUFA diet). According to a randomized cross-over design, 6 type 2 diabetic patients (58±5 years; BMI 27±7 kg/m2 )(M±SD) in satisfactory blood glucose control on diet or diet+hypoglycaemic drugs, followed for one month a diet rich in CHO (52% total energy) and fibre (28g/1000 kcal) and with low glycaemic index (GI) (58%) or a diet low in CHO (45% total energy) and fibre (8g/1000 kcal) and with high GI (88%), and then crossed over to the alternate diet for another month. At the end of each period, a test meal of the same composition of the corresponding diet was administered and plasma samples were taken before and over 6 hrs after meal for determination of lipids and lipoprotein fractions (separated by discontinuous density gradient ultracentrifugation). Cholesterol (Chol) and triglycerides (Tg) concentrations in either chylomicrons and large VLDL were significantly lower at the end of the high-CHO/high-fibre/low-GI diet compared to the other diet for the whole postprandial curve, with lower incremental AUCs of chylomicron (Chol 1.80±0.60 vs. 3.99±1.82 mg/dl 6h, p<0.01; Tg 55±25 vs. 93±38 mg/dl 6h, p=0.009) and large VLDL (Chol 10±16 vs. 30±17 mg/dl 6h, p=0.03; Tg 108±78 vs 214±120 mg/dl 6h, p=0.03). In conclusion, a high-CHO/high-fibre/low-GI diet is able to significantly improve the postprandial response of TG-rich lipoproteins compared to a low-CHO/high-MUFA diet. This kind of diet, also because of its beneficial postprandial effects, may be the diet of choice for diabetic patients.
artery flow-mediated endothelial dependent vasodilatation at baseline and after the intervention. Results: Plant stanol ester intake reduced LDL cholesterol concentration by 9% from baseline (p<0.001) similarly in different treatment groups. Carotid artery compliance or brachial artery flow-mediated vasodilatation were not changed. However, in subjects with low baseline carotid artery compliance, it was changed from 1.23 to 1.59%/10 mmHg in the active treatment groups (n=69) without significant LDL cholesterol changes, and from 1.42 to 1.47%/10mmHg in the control group (n=25), (P=0.0035 between groups). In subjects with low baseline flow-mediated dilatation capacity, it was changed from 6.9% to 8.6% in active treatment groups (n=73), and from 6.6% to 6.8% in control group (n=24) (P=0.05 between groups). Conclusions: Consumption of plant stanol esters is associated with beneficial changes in arterial elasticity and endothelial function in subjects with low baseline values (below population median) irrespective of LDL cholesterol lowering. PO20-616
L. Casani, E. Segales, L. Badimon. Cardiovascular Research Center, CSIC-ICCC, Hospital de Sant Pau, Barcelona, Spain Objectives: Epidemiological and clinical evidences have shown that moderate intake of red wine has cardiovascular protective effects; however, its mechanisms of action are poorly known. We have studied the effects of a daily dose of red wine (Tempranillo, 12.8% alcohol v/v) in blood thrombogenicity under controlled flow conditions in an experiemental model. Methods: Pigs were fed for 21days a growing chow. One group (n=4) received a daily moderate dose of red wine (40g wine ethanol/day) while the other rested as controls (n=4). Thrombosis triggered by vascular injury was evaluated in the previously validated Badimon perfusion chamber by radioisotopic evaluation of deposited platelets. Changes in RhoA translocation, vascular prostacyclin (PGI2) release and plasma lipids were analyzed. Results: Platelet deposition rate (TPD/min of perfusion) was reduced (P<0.05) in animals ingesting red wine compared with control animals in perfusions mimicking blood flow in patent coronary arteries over severely (4,06±0,5 vs. 7,66±1,2 PLT×106 /cm2 ) and mildly (0,66±0,18 vs. 1,54±0,4 PLT×106 /cm2 ) damaged vessel wall. Expression of RhoA in the platelet membrane (active form) was reduced in wine-fed animals (p ≤ 0.05). PGI2 was significantly higher (>50%) in coronaries of wine fed animals. Plasma lipid were not changed. Conclusions: Moderate red wine intake, as the only modification of the regular normolipidemic diet, significantly reduces platelet activation and deposition triggered by damaged arteries, indicating a protective effect against platelet mediated thrombogenicity in an experimental model of human resemblance. Hence, a daily moderate dose of red wine taken with meals, through the regulation of platelet activation, induces vascular protection. PO20-617
PO20-615
IMPAIRED ARTERIAL ELASTICITY AND ENDOTHELIAL FUNCTION ARE IMPROVED WITH CONSUMPTION OF PLANT STANOL ESTERS
O. Raitakari 1 , H. Gylling 2 , P. Salo 3 , T.A. Miettinen 4 . 1 Department of Clinical Physiology, University of Turku, Turku, Finland; 2 Department of Clinical Nutrition, University of Kuopio, Kuopio, Finland; 3 Raisio Benecol, Raisio, Finland; 4 Department of Medicine, University of Helsinki, Helsinki, Finland Background and Aims: LDL cholesterol reduction with statins can improve endothelial dysfunction in hyperlipidemic subjects. Thus, plant stanol (2 g/d) rapeseed oil esters mixed with different vegetable oil margarines were consumed to evaluate whether LDL cholesterol reduction was associated with improved endothelial function. Material and Methods: Two hundred adults with serum cholesterol concentration > 5 mmol/l were randomised to consume camelina oil, rapeseed oil or sunflower oil spread with stanol ester or sunflower oil spread without stanol ester (control group) for 3 months. Non-invasive ultrasound was used to measure carotid artery compliance and brachial
FLOW-MEDIATED PLATELET DEPOSITION ON DAMAGED ARTERIAL WALL IS REDUCED BY DAILY INTAKE OF A MODERATE AMOUNT OF RED WINE
EFFECTS OF FERROSUPER (DRIATEC) SUPPLEMENTATION ON LIPID AND REDOX PARAMETERS IN HEALTHY SUBJECTS
R. Accinni 1 , V. Sedda 1 , C. Dellanoce 1 , M. Rosina 2 , A.F. Speciani 1 , G. Perrone 1 , B. Ostan 1 , D. Vietti 1 . 1 CNR Clinical Physiology Institute-Milan, Cardiology Department, Niguarda Ca Granda Hospital, Milan, Italy; 2 Department of Medical Science, University of Milan, Ospedale Maggiore-IRCCS, Milan, Italy Background and Aims: Relationship between iron and atherosclerosis in vivo is still controversial. This study investigated whether a specific iron supplementation (FerroSuper, 4.2 mg/die of Fe pidolate) affects the redox, lipid and iron parameters in healthy volunteers. Methods: Iron and lipid parameters, blood (b) and plasma (p) total and reduced aminothiols (homocysteine, cysteine, glutathione) and cholesterol normalyzed vitamin E (VitE/cholesterol) were measured in healthy subjects (n = 28) before (T0) and after 4 (T1) and 12 weeks (n = 13, T2) of FerroSuper. Results: At T1, total and reduced (p)homocysteine (p = 0.008 and p = 0.0001 respectively) were decreased; an opposite behaviour was found for
76th Congress of the European Atherosclerosis Society, June 10–13, 2007, Helsinki, Finland
POSTER SESSIONS
AN OPTIMAL DIETARY APPROACH FOR TREATING POSTPRANDIAL LIPID ABNORMALITIES IN TYPE 2 DIABETIC PATIENTS
167
A.A. Rivellese, C. De Natale, L. Patti, L. Bozzetto, P. Massaro, G. Cosatbile, G. Riccardi, G. Annuzzi. Department of Clinical and Experimental Medicine, Federico II University, Naples, Italy To evaluate the effects on postprandial lipaemia of two diets generally recommended for diabetic patients (high-CHO/high-fibre versus low-CHO/high-MUFA diet). According to a randomized cross-over design, 6 type 2 diabetic patients (58±5 years; BMI 27±7 kg/m2 )(M±SD) in satisfactory blood glucose control on diet or diet+hypoglycaemic drugs, followed for one month a diet rich in CHO (52% total energy) and fibre (28g/1000 kcal) and with low glycaemic index (GI) (58%) or a diet low in CHO (45% total energy) and fibre (8g/1000 kcal) and with high GI (88%), and then crossed over to the alternate diet for another month. At the end of each period, a test meal of the same composition of the corresponding diet was administered and plasma samples were taken before and over 6 hrs after meal for determination of lipids and lipoprotein fractions (separated by discontinuous density gradient ultracentrifugation). Cholesterol (Chol) and triglycerides (Tg) concentrations in either chylomicrons and large VLDL were significantly lower at the end of the high-CHO/high-fibre/low-GI diet compared to the other diet for the whole postprandial curve, with lower incremental AUCs of chylomicron (Chol 1.80±0.60 vs. 3.99±1.82 mg/dl 6h, p<0.01; Tg 55±25 vs. 93±38 mg/dl 6h, p=0.009) and large VLDL (Chol 10±16 vs. 30±17 mg/dl 6h, p=0.03; Tg 108±78 vs 214±120 mg/dl 6h, p=0.03). In conclusion, a high-CHO/high-fibre/low-GI diet is able to significantly improve the postprandial response of TG-rich lipoproteins compared to a low-CHO/high-MUFA diet. This kind of diet, also because of its beneficial postprandial effects, may be the diet of choice for diabetic patients.
artery flow-mediated endothelial dependent vasodilatation at baseline and after the intervention. Results: Plant stanol ester intake reduced LDL cholesterol concentration by 9% from baseline (p<0.001) similarly in different treatment groups. Carotid artery compliance or brachial artery flow-mediated vasodilatation were not changed. However, in subjects with low baseline carotid artery compliance, it was changed from 1.23 to 1.59%/10 mmHg in the active treatment groups (n=69) without significant LDL cholesterol changes, and from 1.42 to 1.47%/10mmHg in the control group (n=25), (P=0.0035 between groups). In subjects with low baseline flow-mediated dilatation capacity, it was changed from 6.9% to 8.6% in active treatment groups (n=73), and from 6.6% to 6.8% in control group (n=24) (P=0.05 between groups). Conclusions: Consumption of plant stanol esters is associated with beneficial changes in arterial elasticity and endothelial function in subjects with low baseline values (below population median) irrespective of LDL cholesterol lowering. PO20-616
L. Casani, E. Segales, L. Badimon. Cardiovascular Research Center, CSIC-ICCC, Hospital de Sant Pau, Barcelona, Spain Objectives: Epidemiological and clinical evidences have shown that moderate intake of red wine has cardiovascular protective effects; however, its mechanisms of action are poorly known. We have studied the effects of a daily dose of red wine (Tempranillo, 12.8% alcohol v/v) in blood thrombogenicity under controlled flow conditions in an experiemental model. Methods: Pigs were fed for 21days a growing chow. One group (n=4) received a daily moderate dose of red wine (40g wine ethanol/day) while the other rested as controls (n=4). Thrombosis triggered by vascular injury was evaluated in the previously validated Badimon perfusion chamber by radioisotopic evaluation of deposited platelets. Changes in RhoA translocation, vascular prostacyclin (PGI2) release and plasma lipids were analyzed. Results: Platelet deposition rate (TPD/min of perfusion) was reduced (P<0.05) in animals ingesting red wine compared with control animals in perfusions mimicking blood flow in patent coronary arteries over severely (4,06±0,5 vs. 7,66±1,2 PLT×106 /cm2 ) and mildly (0,66±0,18 vs. 1,54±0,4 PLT×106 /cm2 ) damaged vessel wall. Expression of RhoA in the platelet membrane (active form) was reduced in wine-fed animals (p ≤ 0.05). PGI2 was significantly higher (>50%) in coronaries of wine fed animals. Plasma lipid were not changed. Conclusions: Moderate red wine intake, as the only modification of the regular normolipidemic diet, significantly reduces platelet activation and deposition triggered by damaged arteries, indicating a protective effect against platelet mediated thrombogenicity in an experimental model of human resemblance. Hence, a daily moderate dose of red wine taken with meals, through the regulation of platelet activation, induces vascular protection. PO20-617
PO20-615
IMPAIRED ARTERIAL ELASTICITY AND ENDOTHELIAL FUNCTION ARE IMPROVED WITH CONSUMPTION OF PLANT STANOL ESTERS
O. Raitakari 1 , H. Gylling 2 , P. Salo 3 , T.A. Miettinen 4 . 1 Department of Clinical Physiology, University of Turku, Turku, Finland; 2 Department of Clinical Nutrition, University of Kuopio, Kuopio, Finland; 3 Raisio Benecol, Raisio, Finland; 4 Department of Medicine, University of Helsinki, Helsinki, Finland Background and Aims: LDL cholesterol reduction with statins can improve endothelial dysfunction in hyperlipidemic subjects. Thus, plant stanol (2 g/d) rapeseed oil esters mixed with different vegetable oil margarines were consumed to evaluate whether LDL cholesterol reduction was associated with improved endothelial function. Material and Methods: Two hundred adults with serum cholesterol concentration > 5 mmol/l were randomised to consume camelina oil, rapeseed oil or sunflower oil spread with stanol ester or sunflower oil spread without stanol ester (control group) for 3 months. Non-invasive ultrasound was used to measure carotid artery compliance and brachial
FLOW-MEDIATED PLATELET DEPOSITION ON DAMAGED ARTERIAL WALL IS REDUCED BY DAILY INTAKE OF A MODERATE AMOUNT OF RED WINE
EFFECTS OF FERROSUPER (DRIATEC) SUPPLEMENTATION ON LIPID AND REDOX PARAMETERS IN HEALTHY SUBJECTS
R. Accinni 1 , V. Sedda 1 , C. Dellanoce 1 , M. Rosina 2 , A.F. Speciani 1 , G. Perrone 1 , B. Ostan 1 , D. Vietti 1 . 1 CNR Clinical Physiology Institute-Milan, Cardiology Department, Niguarda Ca Granda Hospital, Milan, Italy; 2 Department of Medical Science, University of Milan, Ospedale Maggiore-IRCCS, Milan, Italy Background and Aims: Relationship between iron and atherosclerosis in vivo is still controversial. This study investigated whether a specific iron supplementation (FerroSuper, 4.2 mg/die of Fe pidolate) affects the redox, lipid and iron parameters in healthy volunteers. Methods: Iron and lipid parameters, blood (b) and plasma (p) total and reduced aminothiols (homocysteine, cysteine, glutathione) and cholesterol normalyzed vitamin E (VitE/cholesterol) were measured in healthy subjects (n = 28) before (T0) and after 4 (T1) and 12 weeks (n = 13, T2) of FerroSuper. Results: At T1, total and reduced (p)homocysteine (p = 0.008 and p = 0.0001 respectively) were decreased; an opposite behaviour was found for
76th Congress of the European Atherosclerosis Society, June 10–13, 2007, Helsinki, Finland
POSTER SESSIONS
AN OPTIMAL DIETARY APPROACH FOR TREATING POSTPRANDIAL LIPID ABNORMALITIES IN TYPE 2 DIABETIC PATIENTS
167