- Email: [email protected]
Protein yield of grain sorghum (Sorghum bicolor (L.) Moench), grown in Southeastern Romania area
S90
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
1 Faculty of Science, Department of Biology/Biotechnology, Hacettepe University, Ankara 06800, Turkey 2 Faculty of Science & Letters, Department of Biology, Aksaray University, Aksaray 68100, Turkey
N0P60K0, 1753 kg ha−1 for N45P60K0, 2325 kg ha−1 for N45P60K45 and 2496 kg ha−1 for N90P60K45. The average yield growth brought by fertilization was: 336 kg ha−1 for N45P60K0, 908 kg ha−1 for N45P60K45 and 1079 kg ha−1 for N90P60K45. Oil content was determined by near infrared spectroscopy with Instalab 600 NIR Product-Analyzer, equipment. The fat content in grains varied depending on variety and fertilizer from 20.3% for PR92B63 and 22.7% for Orion. Average oil production was: 308.2 l ha−1 for N0P60K0, 376 l ha−1 for N45P60K0, 497 l ha−1 for N45P60K45 and 522 l ha−1 for N90P60K45. Applying fertilization helped increase the oil production with values between 12.2% and 69.4%.
E-mail address: [email protected] (K.E. Turkmen).
http://dx.doi.org/10.1016/j.jbiotec.2016.05.320
Determination of quorum sensing signal molecules by bacteria isolated from different food samples Kubra Erkan Turkmen 1,∗ , Demet Erdonmez 2 , Nilufer Aksoz 1
Food spoilage may be cause as undesirable for consumption. It is the outcome of the biochemical activity of a microbial community. Therefore, various information is reported, this activity has been connected to quorum sensing (QS) system. The potential role of cell-to-cell communication system in food safety should be more comprehensively explained. In this study, we aimed to investigate quorum sensing signal molecules of isolated foodborne pathogen. Six different samples were taken from various food. These samples were incubated in LB (Luria Bertani) Broth medium at 37 ◦ C 24 h. Then, 100 l of each sample were taken and inoculated in different selective media (Cetrimide agar, SS agar, King B agar, Mac Conkey agar) to incubate at 37 ◦ C 24 h. After incubation, each of purified colonies were selected to carry out Quorum sensing (QS) assay. For this reason, Chromobacterium violaceum (CV 026) was used as a biosensor strain. QS signal molecules were identified by cross-validation test using CV026. Following process was extraction of QS signal molecules and validation of these molecules by soft agar with CV026. We obtained various pathogen microorganisms from different food samples. It showed spesific growth characteristic in these media that provided to identification bacteria as Pseudomonas sp., Salmonella sp., Escherichia coli, Klebsiella sp. Five of these isolates (2 Pseudomonas sp., 2 E. coli, 1 Salmonella sp.) were found out between C4-C8 AHL (N-Acyl-homoserin lacton) signal molecules that CV026 could use to detect this range of signal molecules. Consequently, this study could provide us to gain a different point of view on development pathogenicity in foodborne or food related pathogen.
Protein yield of grain sorghum (Sorghum bicolor (L.) Moench), grown in Southeastern Romania area Cristina Andreea Oprea ∗ , Doru Ioan Marin, Ciprian Bolohan University of Agronomic Sciences and Veterinary Medicine of Bucharest, Bucharest, Romania E-mail address: [email protected] (C.A. Oprea).
Nicoleta Capatana ∗ , Doru Ioan Marin
Sorghum is a sub-saharan grain with high adaptability to less favorable ecological conditions (pour soils, arid climate), because of its capacity to efficiently use natural resources and increased tolerance to drought. Research aimed to evaluate the suitability of grain sorghum cultivation in the Southeastern area of Romania, in terms of yield (grain and biomass) and grain quality. Research was carried out in 2013–2015 at Moara Domneasca (chromic luvosoil) on three sorghum hybrids (a1-Alize, a2-Aquilon, a3-Arack) and different fertilization levels. Crude protein (CP) content was determined using the Kjeldahl method. This paper includes results obtained for the fertilization levels: b1-N0P0K0 – control, b2-N120P0K0 and b3-N120P60K60. Average grain yield varied under fertilization influence between 5443.2 kg ha−1 (control) and 9233.0 kg ha−1 (N120P60K60), LSD 5% = 553.9 kg ha−1 . Grain CP content varied under hybrid and fertilization influence from 9.27% (Arack – unfertilized) to 13.83% (Alize – fertilized N120P60K60). The highest CP yield was obtained at the hybrid Aquilon for the fertilization level b3 (1278.0 kg ha−1 ). Fertilization usage, through its effects on grain yield and protein content, determined an average CP increase of 582.9 kg ha−1 for b2 fertilization level (208% compared to control) and of 690.6 kg ha−1 for b3 fertilization level (228% compared to control), LSD 5% = 80.0 kg ha−1 . This results show sorghum’s adaptability to the pedological and climatic conditions in the researched area, in terms of grain yield and grain quality.
University of Agronomic Sciences and Veterinary Medicine of Bucharest, Bucharest, Romania
http://dx.doi.org/10.1016/j.jbiotec.2016.05.321
http://dx.doi.org/10.1016/j.jbiotec.2016.05.319 Comparative researches on soybean crop Glycine Max L. (Merrill) on grain and oil yield under the conditions of South East Romania
E-mail address: [email protected] (N. Capatana). Soybean Glycine Max L. (Merrill) is a crop with a wide range of usage and it is frequently used in human alimentation, animal feeding and industry. Soybean ranks first place in the group of grain leguminous plants. Research aimed to evaluate the yield of three soybean varieties, Carla, PR92B63 and Orion, under different doses of fertilizers: N0P60K0, N45P60K0, N45P60K45, N90P60K45. Research has been carried out in Southeastern Romania (44◦ 29 33 N, 26◦ 15 20 E) on a chromic luvosoil. During the growing season climatic conditions (April–September 2015) were characterized by 232 mm rainfalls representing 66.4% of the normal area values, and average temperature was 19.97 ◦ C compared to 18.10 ◦ C, the normal value for this area. Average grain yield for three varieties was: 1417 kg ha−1 for
Solutions for decreasing a crude oil inherent toxicity to bacteria-biodegraders Arturs Eglitis ∗ , Eduards Dokukins, Andrejs Berzins, Olga Muter Institute of Microbiology, University of Latvia Latvia E-mail address: [email protected] (A. Eglitis). Microorganisms with hydrocarbons-degrading activity have a great biotechnological potential. However, the process of biodegradation could be notably inhibited due to an inherent toxicity of crude oil. A broad spectrum of technological solutions has been recently applied worldwide to overcome this limitation. Our study was
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
1 Faculty of Science, Department of Biology/Biotechnology, Hacettepe University, Ankara 06800, Turkey 2 Faculty of Science & Letters, Department of Biology, Aksaray University, Aksaray 68100, Turkey
N0P60K0, 1753 kg ha−1 for N45P60K0, 2325 kg ha−1 for N45P60K45 and 2496 kg ha−1 for N90P60K45. The average yield growth brought by fertilization was: 336 kg ha−1 for N45P60K0, 908 kg ha−1 for N45P60K45 and 1079 kg ha−1 for N90P60K45. Oil content was determined by near infrared spectroscopy with Instalab 600 NIR Product-Analyzer, equipment. The fat content in grains varied depending on variety and fertilizer from 20.3% for PR92B63 and 22.7% for Orion. Average oil production was: 308.2 l ha−1 for N0P60K0, 376 l ha−1 for N45P60K0, 497 l ha−1 for N45P60K45 and 522 l ha−1 for N90P60K45. Applying fertilization helped increase the oil production with values between 12.2% and 69.4%.
E-mail address: [email protected] (K.E. Turkmen).
http://dx.doi.org/10.1016/j.jbiotec.2016.05.320
Determination of quorum sensing signal molecules by bacteria isolated from different food samples Kubra Erkan Turkmen 1,∗ , Demet Erdonmez 2 , Nilufer Aksoz 1
Food spoilage may be cause as undesirable for consumption. It is the outcome of the biochemical activity of a microbial community. Therefore, various information is reported, this activity has been connected to quorum sensing (QS) system. The potential role of cell-to-cell communication system in food safety should be more comprehensively explained. In this study, we aimed to investigate quorum sensing signal molecules of isolated foodborne pathogen. Six different samples were taken from various food. These samples were incubated in LB (Luria Bertani) Broth medium at 37 ◦ C 24 h. Then, 100 l of each sample were taken and inoculated in different selective media (Cetrimide agar, SS agar, King B agar, Mac Conkey agar) to incubate at 37 ◦ C 24 h. After incubation, each of purified colonies were selected to carry out Quorum sensing (QS) assay. For this reason, Chromobacterium violaceum (CV 026) was used as a biosensor strain. QS signal molecules were identified by cross-validation test using CV026. Following process was extraction of QS signal molecules and validation of these molecules by soft agar with CV026. We obtained various pathogen microorganisms from different food samples. It showed spesific growth characteristic in these media that provided to identification bacteria as Pseudomonas sp., Salmonella sp., Escherichia coli, Klebsiella sp. Five of these isolates (2 Pseudomonas sp., 2 E. coli, 1 Salmonella sp.) were found out between C4-C8 AHL (N-Acyl-homoserin lacton) signal molecules that CV026 could use to detect this range of signal molecules. Consequently, this study could provide us to gain a different point of view on development pathogenicity in foodborne or food related pathogen.
Protein yield of grain sorghum (Sorghum bicolor (L.) Moench), grown in Southeastern Romania area Cristina Andreea Oprea ∗ , Doru Ioan Marin, Ciprian Bolohan University of Agronomic Sciences and Veterinary Medicine of Bucharest, Bucharest, Romania E-mail address: [email protected] (C.A. Oprea).
Nicoleta Capatana ∗ , Doru Ioan Marin
Sorghum is a sub-saharan grain with high adaptability to less favorable ecological conditions (pour soils, arid climate), because of its capacity to efficiently use natural resources and increased tolerance to drought. Research aimed to evaluate the suitability of grain sorghum cultivation in the Southeastern area of Romania, in terms of yield (grain and biomass) and grain quality. Research was carried out in 2013–2015 at Moara Domneasca (chromic luvosoil) on three sorghum hybrids (a1-Alize, a2-Aquilon, a3-Arack) and different fertilization levels. Crude protein (CP) content was determined using the Kjeldahl method. This paper includes results obtained for the fertilization levels: b1-N0P0K0 – control, b2-N120P0K0 and b3-N120P60K60. Average grain yield varied under fertilization influence between 5443.2 kg ha−1 (control) and 9233.0 kg ha−1 (N120P60K60), LSD 5% = 553.9 kg ha−1 . Grain CP content varied under hybrid and fertilization influence from 9.27% (Arack – unfertilized) to 13.83% (Alize – fertilized N120P60K60). The highest CP yield was obtained at the hybrid Aquilon for the fertilization level b3 (1278.0 kg ha−1 ). Fertilization usage, through its effects on grain yield and protein content, determined an average CP increase of 582.9 kg ha−1 for b2 fertilization level (208% compared to control) and of 690.6 kg ha−1 for b3 fertilization level (228% compared to control), LSD 5% = 80.0 kg ha−1 . This results show sorghum’s adaptability to the pedological and climatic conditions in the researched area, in terms of grain yield and grain quality.
University of Agronomic Sciences and Veterinary Medicine of Bucharest, Bucharest, Romania
http://dx.doi.org/10.1016/j.jbiotec.2016.05.321
http://dx.doi.org/10.1016/j.jbiotec.2016.05.319 Comparative researches on soybean crop Glycine Max L. (Merrill) on grain and oil yield under the conditions of South East Romania
E-mail address: [email protected] (N. Capatana). Soybean Glycine Max L. (Merrill) is a crop with a wide range of usage and it is frequently used in human alimentation, animal feeding and industry. Soybean ranks first place in the group of grain leguminous plants. Research aimed to evaluate the yield of three soybean varieties, Carla, PR92B63 and Orion, under different doses of fertilizers: N0P60K0, N45P60K0, N45P60K45, N90P60K45. Research has been carried out in Southeastern Romania (44◦ 29 33 N, 26◦ 15 20 E) on a chromic luvosoil. During the growing season climatic conditions (April–September 2015) were characterized by 232 mm rainfalls representing 66.4% of the normal area values, and average temperature was 19.97 ◦ C compared to 18.10 ◦ C, the normal value for this area. Average grain yield for three varieties was: 1417 kg ha−1 for
Solutions for decreasing a crude oil inherent toxicity to bacteria-biodegraders Arturs Eglitis ∗ , Eduards Dokukins, Andrejs Berzins, Olga Muter Institute of Microbiology, University of Latvia Latvia E-mail address: [email protected] (A. Eglitis). Microorganisms with hydrocarbons-degrading activity have a great biotechnological potential. However, the process of biodegradation could be notably inhibited due to an inherent toxicity of crude oil. A broad spectrum of technological solutions has been recently applied worldwide to overcome this limitation. Our study was