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PUB079 EGFR and BRAF Mutation Detection in FFPE Samples of Lung Adenocarcinoma
January 2017
therapy (NRT) in helping smokers quit smoking. There is no other study comparing the abstinence effectiveness of these medications. In Russia, cytisine and NRT are widely available as over-the-counter medications. A real-life study could provide clinicians/researchers with useful information as to the effectiveness of these medications among the general population. The present study aimed to compare past year 30-day and 90-day self-reported smoking abstinence rates for self-reported cytisine and NRT use in individuals who wanted to quit smoking, using a representative sample of the Russian Federation population. Methods: We selected individuals who have tried to quit smoking and who have used cytisine (n ¼88) or NRT (n¼186) in the past year from a Russian nationally representative household survey of adults 15 years of age or older - 2009 Russian Global Adult Tobacco Survey (GATS). All analyses were performed in STATA software taking into account complex survey design features. Multivariate logistic regression models were used to compare past-year use of cytisine and NRT for 30-day and 90-day self-reported smoking abstinence rates. Results: The NRT 30-day and 90-day self-reported smoking abstinence rates were 36.2% and 10.9%, respectively. The cytisine 30-day and 90-day self-reported smoking abstinence rates were 50.4% and 25.3%, respectively. After adjusting the logistic regression for other possible confounders, such as sociodemographic, smoking behavior and treatment variables, cytisine was more effective than NRT only for the 90-day smoking abstinence rate. Conclusion: Just one trial has compared cytisine with NRT. In this context, it is important to verify the superiority of cytisine on NRT for achieving 90-day smoking abstinence, using real-life data from a representative sample of the general population of a country where cytisine and NRT are widely available as over-thecounter medications. These results and those from previous studies call for cytisine licensing worldwide, especially outside Eastern Europe. Keywords: Smoking Cessation, cytisine, epidemiology, nicotine replacement treatment
PUB078 Difficulties in Providing Palliative Care for Advanced Lung Cancer Patients in Rural India (West Bengal) - Experience of an NGO Aditya Manna Oncology, Mas Clinic & Hospital, Tamluk/ India Background: As in any developing countries state of West Bengal in India has a huge burden of metastatic
Abstracts
S1493
Lung cancer patients in advanced stage coming from rural area where awareness regarding the usefulness of palliative care in rather poor. Our goal is to give a pain free good quality of life in these advanced stage Lung cancer patients. Objective of this study is to identify the main difficulties in achieving the above goal in a rural village setting in India. Methods: Advanced Lung cancer patients in need of palliative care in various villages in of rural India were selected for this study. Their symptoms and managements in that rural surroundings were evaluated by an NGO (under the guidance of a senior palliative care specialist) working in that area. An attempt was made to identify the main obstacles in getting proper palliative care in a rural setting. Results: Pain, fatigue, distress, effusion are the main symptoms effecting these patients. In most patients pain and other symptoms control were grossly inadequate due to lack of properly trained manpower in the rural India. However regular homecare visits by a group of social workers were of immense help in the last few months of life. NGO team was well guided by a palliative care specialist. Conclusion: There is a wide gap of trained manpower in this filled in rural areas of India. Dedicated groups from rural area itself need encouragement, repeated home visit, awareness built up, proper training to home care giver, so that difficult symptoms can be managed locally along with necessary social and psychological support to these patients. Keywords: palliative care, NGO, supportive care, rural india
PUB079 EGFR and BRAF Mutation Detection in FFPE Samples of Lung Adenocarcinoma Yang Yang,1 Zhang Hang,2 Shen Xiaoyan,2 Yu Lixia,3 Liu Baorui,3 Wang Lifeng3 1Nanjing Xianlin Drum Tower Hospital, Nanjing/China, 2The Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing/China, 3The Comprehensive Cancer Center of Drum-Tower Hospital, Nanjing University Medical School & Clinical Cancer Institute of Nanjing University, Nanjing/China Background: EGFR-TKIs have been the standard firstline treatment for metastatic NSCLC patients with sensitive EGFR-mutants, meanwhile, other drugs target different driver mutants like BRAF-inhibitor dabrafenib has shown promising efficacy for metastatic BRAF-
S1494
mutated NSCLC patients. Therefore, spotting patients carrying the targeted mutations is of great significance. However, molecular detection methods applied in clinical practice, especially detection of BRAF in NSCLC patients needs further exploration. Therefore, more sensitive and economic methods are required. Methods: We applied the CastPCR technology to the molecular detection of EGFR (del2235-2249, del22362250, T790M, L858R) and BRAF (V600E, G469A, D594G) mutations in 144 treatment-naive adenocarcinomas of the lung, and analyzed the association between the mutation rates and patients’ clinicopathological features. Results: EGFR mutation: 74 (51.4%, 74/144) cases were identified harboring EGFR mutations. 58 (40.3%, 58/144) carried sensitizing mutations (19Del or L858R) and 21 (14.6%, 21/144) carried resistant mutations (T790M). 10 (6.9%, 10/144) mutation-positive patients were doublemutated. EGFR mutation rate was significantly higher in female compared with that of males (60.9% v.s. 43.8%, P＜0.05), in non-smokers compared with that of smokers (62.8% v.s. 34.5%, P＜0.05). BRAF mutation:12 (8.3%, 12/144) patients were identified with BRAF mutations. 2 were V600E (16.7%, 2/12) and 10 (83.3%, 10/12) were non-V600E. Among the 10 non-V600E mutations, D594G accounted for 9 (90.0%，9/10) and G469A accounted for only 1 (10.0%, 1/10). Statistical analysis demonstrated that BRAF mutation rate was not associated with any of the clinicopathological features: age, gender, smoking status, differentiation degree, tumor size, regional lymph nodes metastasis and distant metastasis of (P0.05). Conclusion: The CastPCR technology is a robust method with high sensitivity for molecular detecting of EGFR and BRAF mutations in clinical FFPE samples. Keywords: EGFR, CastPCR, BRAF, lung adenocarcinoma
PUB080 Different Methods for Tobacco Hazards Awareness Dilip Acharya Hon.Secretary, Cancer Society of Madhya Pradeshl, Indore/India Background: In the FCTC & MPOWER policy of WHO -W stands to Warn about hazards of Tobacco, specially the health hazards. Tobacco control is complex problem requires attention of all FCTC parties and NGO. Methods: Electronic media-specially TV media has the highest visibility, but, non aired mass media methods for creating awareness, has its own importance and many a times it is much cheaper and gets free coverage & easily available in different languages. Electronic mediaspecially TV media has the highest visibility, but, non aired
Journal of Thoracic Oncology
Vol. 12 No. 1S
mass media methods for creating awareness, has its own importance and many a times it is much cheaper and gets free coverage & easily available in different languages. Cancer Society of M.P. has been working in the field of Tobacco control for many years, using a combination of these different methodologies. Some of the activities conducted by us for Tobacco control and creating awareness about its health hazards are:- Posters: We have prepared, published and distributed various posters for tobacco control and self examination of oral cavity. Our posters have won international awards and have also been presented in many national & international conferences. The release of poster by eminent personalities, politicians gives us free media coverage on the topic too. One of our posters was also tested by Americans for its impact in Egypt. Hoardings/Bill boards are a cheaper long term display media, strategically placed, it has a high visibility also.Pamphlets:- We have distributed nearly 100 thousand pamphlets in the holy Sinhastha Mela (fair) where nearly 10 million people participated. Hoardings/Bill Boards:- Installed at strategic points they are a source of constant reminder to th health hazards of tobacco. Lectures/symposiums:- We regularly give lectures on different aspects of tobacco control & its health hazards. Exhibitions:- Participation & display of banners,posters etc in various exhibitions, where many people come & see them. Public Rally:- Rally taken out through the city draws attention of public as well as gets free media coverage. Involvement of Priests:-lectures given in various temples & holy places to involve priests into tobacco control. Lectures/symposiums/workshops- we have done more then 100 such activities, mainly targeting schools, colleges, social clubs, police, media, lawyers etc. Car Stickers, pasting of small banners behind rickshaws, slogan contests & involvement of local service clubs in the activities helps us reach a larger population. Results: All media are helpful in Tobacco Control. Conclusion: Although TV is an important awareness media, others are also important. Keywords: Tobacco, Media
PUB081 Discovery of 1,3,5-Triazine Based Novel EGFR-Tyrosine Kinase Inhibitor against Human Lung Carcinoma Udaya Singh,1 Hans Bhat2 1Drug Design & Discovery Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences, Allahabad/India, 2Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh/India
therapy (NRT) in helping smokers quit smoking. There is no other study comparing the abstinence effectiveness of these medications. In Russia, cytisine and NRT are widely available as over-the-counter medications. A real-life study could provide clinicians/researchers with useful information as to the effectiveness of these medications among the general population. The present study aimed to compare past year 30-day and 90-day self-reported smoking abstinence rates for self-reported cytisine and NRT use in individuals who wanted to quit smoking, using a representative sample of the Russian Federation population. Methods: We selected individuals who have tried to quit smoking and who have used cytisine (n ¼88) or NRT (n¼186) in the past year from a Russian nationally representative household survey of adults 15 years of age or older - 2009 Russian Global Adult Tobacco Survey (GATS). All analyses were performed in STATA software taking into account complex survey design features. Multivariate logistic regression models were used to compare past-year use of cytisine and NRT for 30-day and 90-day self-reported smoking abstinence rates. Results: The NRT 30-day and 90-day self-reported smoking abstinence rates were 36.2% and 10.9%, respectively. The cytisine 30-day and 90-day self-reported smoking abstinence rates were 50.4% and 25.3%, respectively. After adjusting the logistic regression for other possible confounders, such as sociodemographic, smoking behavior and treatment variables, cytisine was more effective than NRT only for the 90-day smoking abstinence rate. Conclusion: Just one trial has compared cytisine with NRT. In this context, it is important to verify the superiority of cytisine on NRT for achieving 90-day smoking abstinence, using real-life data from a representative sample of the general population of a country where cytisine and NRT are widely available as over-thecounter medications. These results and those from previous studies call for cytisine licensing worldwide, especially outside Eastern Europe. Keywords: Smoking Cessation, cytisine, epidemiology, nicotine replacement treatment
PUB078 Difficulties in Providing Palliative Care for Advanced Lung Cancer Patients in Rural India (West Bengal) - Experience of an NGO Aditya Manna Oncology, Mas Clinic & Hospital, Tamluk/ India Background: As in any developing countries state of West Bengal in India has a huge burden of metastatic
Abstracts
S1493
Lung cancer patients in advanced stage coming from rural area where awareness regarding the usefulness of palliative care in rather poor. Our goal is to give a pain free good quality of life in these advanced stage Lung cancer patients. Objective of this study is to identify the main difficulties in achieving the above goal in a rural village setting in India. Methods: Advanced Lung cancer patients in need of palliative care in various villages in of rural India were selected for this study. Their symptoms and managements in that rural surroundings were evaluated by an NGO (under the guidance of a senior palliative care specialist) working in that area. An attempt was made to identify the main obstacles in getting proper palliative care in a rural setting. Results: Pain, fatigue, distress, effusion are the main symptoms effecting these patients. In most patients pain and other symptoms control were grossly inadequate due to lack of properly trained manpower in the rural India. However regular homecare visits by a group of social workers were of immense help in the last few months of life. NGO team was well guided by a palliative care specialist. Conclusion: There is a wide gap of trained manpower in this filled in rural areas of India. Dedicated groups from rural area itself need encouragement, repeated home visit, awareness built up, proper training to home care giver, so that difficult symptoms can be managed locally along with necessary social and psychological support to these patients. Keywords: palliative care, NGO, supportive care, rural india
PUB079 EGFR and BRAF Mutation Detection in FFPE Samples of Lung Adenocarcinoma Yang Yang,1 Zhang Hang,2 Shen Xiaoyan,2 Yu Lixia,3 Liu Baorui,3 Wang Lifeng3 1Nanjing Xianlin Drum Tower Hospital, Nanjing/China, 2The Comprehensive Cancer Center of Drum-Tower Hospital, Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing/China, 3The Comprehensive Cancer Center of Drum-Tower Hospital, Nanjing University Medical School & Clinical Cancer Institute of Nanjing University, Nanjing/China Background: EGFR-TKIs have been the standard firstline treatment for metastatic NSCLC patients with sensitive EGFR-mutants, meanwhile, other drugs target different driver mutants like BRAF-inhibitor dabrafenib has shown promising efficacy for metastatic BRAF-
S1494
mutated NSCLC patients. Therefore, spotting patients carrying the targeted mutations is of great significance. However, molecular detection methods applied in clinical practice, especially detection of BRAF in NSCLC patients needs further exploration. Therefore, more sensitive and economic methods are required. Methods: We applied the CastPCR technology to the molecular detection of EGFR (del2235-2249, del22362250, T790M, L858R) and BRAF (V600E, G469A, D594G) mutations in 144 treatment-naive adenocarcinomas of the lung, and analyzed the association between the mutation rates and patients’ clinicopathological features. Results: EGFR mutation: 74 (51.4%, 74/144) cases were identified harboring EGFR mutations. 58 (40.3%, 58/144) carried sensitizing mutations (19Del or L858R) and 21 (14.6%, 21/144) carried resistant mutations (T790M). 10 (6.9%, 10/144) mutation-positive patients were doublemutated. EGFR mutation rate was significantly higher in female compared with that of males (60.9% v.s. 43.8%, P＜0.05), in non-smokers compared with that of smokers (62.8% v.s. 34.5%, P＜0.05). BRAF mutation:12 (8.3%, 12/144) patients were identified with BRAF mutations. 2 were V600E (16.7%, 2/12) and 10 (83.3%, 10/12) were non-V600E. Among the 10 non-V600E mutations, D594G accounted for 9 (90.0%，9/10) and G469A accounted for only 1 (10.0%, 1/10). Statistical analysis demonstrated that BRAF mutation rate was not associated with any of the clinicopathological features: age, gender, smoking status, differentiation degree, tumor size, regional lymph nodes metastasis and distant metastasis of (P0.05). Conclusion: The CastPCR technology is a robust method with high sensitivity for molecular detecting of EGFR and BRAF mutations in clinical FFPE samples. Keywords: EGFR, CastPCR, BRAF, lung adenocarcinoma
PUB080 Different Methods for Tobacco Hazards Awareness Dilip Acharya Hon.Secretary, Cancer Society of Madhya Pradeshl, Indore/India Background: In the FCTC & MPOWER policy of WHO -W stands to Warn about hazards of Tobacco, specially the health hazards. Tobacco control is complex problem requires attention of all FCTC parties and NGO. Methods: Electronic media-specially TV media has the highest visibility, but, non aired mass media methods for creating awareness, has its own importance and many a times it is much cheaper and gets free coverage & easily available in different languages. Electronic mediaspecially TV media has the highest visibility, but, non aired
Journal of Thoracic Oncology
Vol. 12 No. 1S
mass media methods for creating awareness, has its own importance and many a times it is much cheaper and gets free coverage & easily available in different languages. Cancer Society of M.P. has been working in the field of Tobacco control for many years, using a combination of these different methodologies. Some of the activities conducted by us for Tobacco control and creating awareness about its health hazards are:- Posters: We have prepared, published and distributed various posters for tobacco control and self examination of oral cavity. Our posters have won international awards and have also been presented in many national & international conferences. The release of poster by eminent personalities, politicians gives us free media coverage on the topic too. One of our posters was also tested by Americans for its impact in Egypt. Hoardings/Bill boards are a cheaper long term display media, strategically placed, it has a high visibility also.Pamphlets:- We have distributed nearly 100 thousand pamphlets in the holy Sinhastha Mela (fair) where nearly 10 million people participated. Hoardings/Bill Boards:- Installed at strategic points they are a source of constant reminder to th health hazards of tobacco. Lectures/symposiums:- We regularly give lectures on different aspects of tobacco control & its health hazards. Exhibitions:- Participation & display of banners,posters etc in various exhibitions, where many people come & see them. Public Rally:- Rally taken out through the city draws attention of public as well as gets free media coverage. Involvement of Priests:-lectures given in various temples & holy places to involve priests into tobacco control. Lectures/symposiums/workshops- we have done more then 100 such activities, mainly targeting schools, colleges, social clubs, police, media, lawyers etc. Car Stickers, pasting of small banners behind rickshaws, slogan contests & involvement of local service clubs in the activities helps us reach a larger population. Results: All media are helpful in Tobacco Control. Conclusion: Although TV is an important awareness media, others are also important. Keywords: Tobacco, Media
PUB081 Discovery of 1,3,5-Triazine Based Novel EGFR-Tyrosine Kinase Inhibitor against Human Lung Carcinoma Udaya Singh,1 Hans Bhat2 1Drug Design & Discovery Laboratory, Department of Pharmaceutical Sciences, Sam Higginbottom Institute of Agriculture, Technology & Sciences, Allahabad/India, 2Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh/India