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Regeneration of plantlet from protoplast of Crocus sativus L.
New Biotechnology · Volume 25S · September 2009
ABSTRACTS
3.2.41
3.2.43
Regeneration of plantlet from protoplast of Crocus sativus L.
High antioxidant potential of Fucus spiralis extracts collected from Peniche coast (Portugal)
M. Ahouran 1,∗ , R. Hosseini 1 , R. Zarghami 2
S. Pintéus ∗ , S. Azevedo, C. Alves, T. Mouga, A. Cruz, C. Afonso, M.M. Sampaio, A.I. Rodrigues, R. Pedrosa
1 Department of Agricultural Biotechnology Engineering, College of Engineering and Technology, Imam Khomeini International University, Qazvin, Islamic Republic of Iran 2 Department of Tissue culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Islamic Republic of Iran
Saffron is a triploid and sterile crop that propagated by replanting rest corms thereby it is difficult that we can improvement properties of its agronomic by old methods. Protoplast culture is an efficient instrument for improvement properties of its agronomic. In monocots such as saffron plantlet regeneration from protoplast that isolated directly from the plant’s organs is difficult therefore embryogenic callus proved to be the best source for protoplast isolation. Protoplast isolation was directly from embryogenic callus for 3 h in an enzyme solution consisting of MS medium, 0.2% Pectolyase Y 2—3, 2% Cellulase RS, 0.5% Deriselase, 0.05% MES, and 0.3 M mannitol in the darkness at 22 ◦ C. Isolated protoplast was mixed slowly with steril sodium alginate and was added drop by drop with a sterile pasteur pipette to liquid MS medium which was contained CaCl2 1% and 0.3 M manitol to create Ca—alginate beads. The beads were transferred to MS liquid medium culture with different concentration of growth regulators. After 4—5 weeks of culture, microcalli appeared on the surface of the Ca—alginate beads. Plantlet regeneration was occurred when beads transferred onto solid MS medium without growth regulators with 6% sucrose after 6 months. doi:10.1016/j.nbt.2009.06.674
3.2.42 In vitro micropropagation of Crocus cancellatus M. Ahouran 1,∗ , R. Hosseini 1 , R. Zarghami 2 1 Department of Agricultural Biotechnology Engineering, College of Engineering and Technology, Imam Khomeini International University, Qazvin, Islamic Republic of Iran 2 Department of Tissue culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Islamic Republic of Iran
Crocus spp. is a big genus with a wide range of colours can be developed as new ornamental crop plants, but to date there is not much information on in vitro propagation of this genus except Crocus sativus. In vitro techniques have been used for the micropropagation of various plant species. The present study involves in vitro propagation of Crocus cancellatus. Corm segments were used as starting material for micro-propagation. The best response in micro-corms multiplication was obtained in Murashige and Skoog’s basal medium (MS) supplemented with 3% sucrose and 2 mg l−1 NAA and 1 mg l−1 BAP. The formed micro-corms transplanted directly into soil after storage at 4 ◦ C for 1 month. doi:10.1016/j.nbt.2009.06.675
S296
www.elsevier.com/locate/nbt
Polytechnic Institute of Leiria (IPL), Peniche, Portugal
Natural sources of antioxidants compounds can be used as a cosmetic product or as food conserver by the ability to blunt the lipid peroxidation process. It was reported that some algae have a high concentration of polyphenols such as catechin, epicatechin, epigallocatechin gallate and acid galic. However, many types of algae still to be investigated. Moreover, Portugal is a country that has an enormously and rich marine coast that is largely unexplored. The present study evaluated the antioxidant potential of eight algae (Rhodophyta: Asparagopsis armata, Plocamium cartilagineum and Sphaerococcus coronopifolius; Pheaophyta: Saccorhiza polyschides, Stypocaulon scoparium, Halopteris filicina and Fucus spiralis; Chlorophyta: Codium adhaerens) founded in Peniche coast an in Berlenga Nature Reserve (Portugal). The algae antioxidant potential was evaluated both by quantification of the total polyphenol contents and by DPPH radical scavenging activity. The polyphenol content was measured by the Folin—Ciocalteu method adapted to a microscale. The absorbance was read at 750 nm, and the total polyphenol concentration was calculated from a calibration curve, using gallic acid as a standard. For the DPPH (0.1 mM) radical scavenging assay the absorbance was measured at 517 nm, using BHT as a standard. The antioxidant potential was measured in three different polarity fractions of each algae extract, namely the methanol, dichloromethane and hexane fractions. Methanolic fraction of Fucus spiralis shown the highest polyphenolic contents (90.01 ± 0.8 mg gallic acid equivalent/ g extract) among all fractions of all algae studied. Our results also indicated that Fucus spiralis methanolic fraction has the higher DPPH radical scavenging activity that showed a half of the potency when compared with a pure BHT solution (44.56 ± 0.4% of absorbance reduction; 100 g extract/ml vs 86. 1 ± 1.74% of absorbance reduction; 100 g BHT/ml). It is concluded that Fucus spiralis have the higher antioxidant potential which is related with both high polyphenol content and high radical scavenging capacity. doi:10.1016/j.nbt.2009.06.676
ABSTRACTS
3.2.41
3.2.43
Regeneration of plantlet from protoplast of Crocus sativus L.
High antioxidant potential of Fucus spiralis extracts collected from Peniche coast (Portugal)
M. Ahouran 1,∗ , R. Hosseini 1 , R. Zarghami 2
S. Pintéus ∗ , S. Azevedo, C. Alves, T. Mouga, A. Cruz, C. Afonso, M.M. Sampaio, A.I. Rodrigues, R. Pedrosa
1 Department of Agricultural Biotechnology Engineering, College of Engineering and Technology, Imam Khomeini International University, Qazvin, Islamic Republic of Iran 2 Department of Tissue culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Islamic Republic of Iran
Saffron is a triploid and sterile crop that propagated by replanting rest corms thereby it is difficult that we can improvement properties of its agronomic by old methods. Protoplast culture is an efficient instrument for improvement properties of its agronomic. In monocots such as saffron plantlet regeneration from protoplast that isolated directly from the plant’s organs is difficult therefore embryogenic callus proved to be the best source for protoplast isolation. Protoplast isolation was directly from embryogenic callus for 3 h in an enzyme solution consisting of MS medium, 0.2% Pectolyase Y 2—3, 2% Cellulase RS, 0.5% Deriselase, 0.05% MES, and 0.3 M mannitol in the darkness at 22 ◦ C. Isolated protoplast was mixed slowly with steril sodium alginate and was added drop by drop with a sterile pasteur pipette to liquid MS medium which was contained CaCl2 1% and 0.3 M manitol to create Ca—alginate beads. The beads were transferred to MS liquid medium culture with different concentration of growth regulators. After 4—5 weeks of culture, microcalli appeared on the surface of the Ca—alginate beads. Plantlet regeneration was occurred when beads transferred onto solid MS medium without growth regulators with 6% sucrose after 6 months. doi:10.1016/j.nbt.2009.06.674
3.2.42 In vitro micropropagation of Crocus cancellatus M. Ahouran 1,∗ , R. Hosseini 1 , R. Zarghami 2 1 Department of Agricultural Biotechnology Engineering, College of Engineering and Technology, Imam Khomeini International University, Qazvin, Islamic Republic of Iran 2 Department of Tissue culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Islamic Republic of Iran
Crocus spp. is a big genus with a wide range of colours can be developed as new ornamental crop plants, but to date there is not much information on in vitro propagation of this genus except Crocus sativus. In vitro techniques have been used for the micropropagation of various plant species. The present study involves in vitro propagation of Crocus cancellatus. Corm segments were used as starting material for micro-propagation. The best response in micro-corms multiplication was obtained in Murashige and Skoog’s basal medium (MS) supplemented with 3% sucrose and 2 mg l−1 NAA and 1 mg l−1 BAP. The formed micro-corms transplanted directly into soil after storage at 4 ◦ C for 1 month. doi:10.1016/j.nbt.2009.06.675
S296
www.elsevier.com/locate/nbt
Polytechnic Institute of Leiria (IPL), Peniche, Portugal
Natural sources of antioxidants compounds can be used as a cosmetic product or as food conserver by the ability to blunt the lipid peroxidation process. It was reported that some algae have a high concentration of polyphenols such as catechin, epicatechin, epigallocatechin gallate and acid galic. However, many types of algae still to be investigated. Moreover, Portugal is a country that has an enormously and rich marine coast that is largely unexplored. The present study evaluated the antioxidant potential of eight algae (Rhodophyta: Asparagopsis armata, Plocamium cartilagineum and Sphaerococcus coronopifolius; Pheaophyta: Saccorhiza polyschides, Stypocaulon scoparium, Halopteris filicina and Fucus spiralis; Chlorophyta: Codium adhaerens) founded in Peniche coast an in Berlenga Nature Reserve (Portugal). The algae antioxidant potential was evaluated both by quantification of the total polyphenol contents and by DPPH radical scavenging activity. The polyphenol content was measured by the Folin—Ciocalteu method adapted to a microscale. The absorbance was read at 750 nm, and the total polyphenol concentration was calculated from a calibration curve, using gallic acid as a standard. For the DPPH (0.1 mM) radical scavenging assay the absorbance was measured at 517 nm, using BHT as a standard. The antioxidant potential was measured in three different polarity fractions of each algae extract, namely the methanol, dichloromethane and hexane fractions. Methanolic fraction of Fucus spiralis shown the highest polyphenolic contents (90.01 ± 0.8 mg gallic acid equivalent/ g extract) among all fractions of all algae studied. Our results also indicated that Fucus spiralis methanolic fraction has the higher DPPH radical scavenging activity that showed a half of the potency when compared with a pure BHT solution (44.56 ± 0.4% of absorbance reduction; 100 g extract/ml vs 86. 1 ± 1.74% of absorbance reduction; 100 g BHT/ml). It is concluded that Fucus spiralis have the higher antioxidant potential which is related with both high polyphenol content and high radical scavenging capacity. doi:10.1016/j.nbt.2009.06.676