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Regional thickness of the parietal bone in Korean adults
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026B - Maxillofacial reconstruction
indices of histology and histomorphometry in the distal mandibular body of ovariectomized rabbits were detected as early as 4 weeks, and were marked at 12 weeks as well as at 24 weeks postovariectomy. The results indicate a close association between loss of ovarian function and changes of the mandibular bone in ovariectomized rabbits.
4. Three Dimensional Reconstruction of Oral Mucosal Epithelium for Development of Autograft Procedure and its Biologic Properties
Cha, L, Yook, J., Lee, E., Jeong, S., Park, J., Kim, J. Oral Cancer Research Institute, Department of Oral and Maxillofacial Surgery, Department of Oral Pathology, College of Dentistry, Yonsei University, Seoul, Korea A living-mucosal equivalent is useful in epidermal plastic surgery, and as a model system for basic studies. It is difficult to reconstitute the three-dimensional structure of oral epithelium by conventional culture technique. In this study, we reconstituted the oral mucosal epithelium using organotypic culture with extracellular matrix which mediate the epithelial-mesenchymal interaction. Primary culture was undertaken using a 3T3 feeder layer and epithelium was reconstituted on the dermal equivalents consisting of fibroblast and t y p e I collagen. After growing under an air-liquid surface for 2 weeks, morphologic and biologic characteristics were evaluated. We also assessed the effect of type IV collagen and laminin on the dermal equivalent during the epithelial reconstitution. Isografted reconstituted oral epithelium on nude mice(balb/c) were evaluated histologically and immunohistochemically. Epithelia on the dermal equivalent showed multilayered stratified squamous epithelium which has a reverse polarity. Each of type IV collagen and laminin induce dyskeratotic cells and protrusion int~o the collagen. Molar-ratio mixtures of both matrices induce the well-organized epithelium to show hemidesmosomes and basal lamina as well as similar immunohistochemical characteristics to in vivo. Grafted tissue on the nude mice show similar morphologic and biologic characteristics. These results suggest that reconstitute epithelium with type IV collagen and laminin might be a useful for epithelial graft and experimental model. [This work was supported by Korea Science and Engineering Foundation (981-0707-064-1).]
cal study by measuring the regional thickness of the parietal bone on 30 Korean adult dry skulls. Before the sectioning the calvarium the anatomical landmarks were marked on each specimens. And then we measured the total thickness of the parietal bone and the thickness of the outer and inner cortical plates on various points in each sections of parietal bones using a digital caliper under the stereomicroscope. The total thickness of the parietal bone was ranged from 5.1 mm to 7.5 mm, and there was no statistical difference in the total thickness of the parietal bone on the same points bilaterally. But there was a tendency that the thickness of the parietal bone was thicker toward to the lambda point than the coronal suture area. At the other hand, the thickness of outer and inner plate of the parietal bone was the thickest at the first point of the right aspect on the line 1, the first point of the left aspect on the line 5, respectively. In conclusion, this study showed that the donor site of the parietal bone for the maxillofacial reconstruction should be located -at more posterior and medial area of parietal bone than the prevalent donor site.
6. The Value of Computer-Aided Planning and Intraoperative Navigation in Orbital Reconstruction
Gellrich, N.., Schramm, A., Hammer, B., Schmelzeisen, R. Department of Oral and Maxillofacial Surgery, University of Freiburg, Freiburg, Germany Subject Reconstruction of posttraumatic orbital and periorbital deformities are a surgical challenge. The predictive and intraoperative advantage concerning postsurgical outcome by using modern navigation systems is investigated in 7 (peri-) orbital posttraumatic defects and an optic nerve decompression at the optic canal. Method Spiral computed tomography data of patients were acquired with Siemens Somatom Plus4 and transferred to STN-Navigation-System (Leibingeri/Zeiss). Periorbital bony and soft tissue contours, orbital contents, angles and distances were measured preoperatively on the workstation in a multiplanar view. Furthermore the reconstruction was virtually designed by mirroring the unaffected side to the deformed side. Intraoperatively, contours of preoperative planning were navigated by the surgeon using the STN workstation together with infrared cameras.
5. Regional Thickness of the Parietal Bone in Korean Adults
Choi, S. a, Cha, L 1, Kim, H), Jeong, E 1, Yi, C ~, Jeong, 1.3 1Department of Oral and Maxillofacial Surgery, 2Department of Oral Biology, College of Dentistry, 3Department of Anatomy, College of Medicine, Yonsei University To clarify the clinical utility of the calavarial bone graft in the maxillofacial reconstruction, we performed an anatomi-
Results In 7 cases of orbital reconstruction (enophthalmus correction, malar bone augmentation) surgical steps could be performed precisely as preoperatively planned on the workstation. Autologous bone could be harvested and molded to the demands of the surgeon to cover or augment defects of the orbit and periorbital region. In posttraumatic optic nerve decompression at the optic canal the surgical route could be exactly planned and the surgeon could be securely guided to the region of interest i.e. the lateral wall of the sphenoid sinus.
026B - Maxillofacial reconstruction
indices of histology and histomorphometry in the distal mandibular body of ovariectomized rabbits were detected as early as 4 weeks, and were marked at 12 weeks as well as at 24 weeks postovariectomy. The results indicate a close association between loss of ovarian function and changes of the mandibular bone in ovariectomized rabbits.
4. Three Dimensional Reconstruction of Oral Mucosal Epithelium for Development of Autograft Procedure and its Biologic Properties
Cha, L, Yook, J., Lee, E., Jeong, S., Park, J., Kim, J. Oral Cancer Research Institute, Department of Oral and Maxillofacial Surgery, Department of Oral Pathology, College of Dentistry, Yonsei University, Seoul, Korea A living-mucosal equivalent is useful in epidermal plastic surgery, and as a model system for basic studies. It is difficult to reconstitute the three-dimensional structure of oral epithelium by conventional culture technique. In this study, we reconstituted the oral mucosal epithelium using organotypic culture with extracellular matrix which mediate the epithelial-mesenchymal interaction. Primary culture was undertaken using a 3T3 feeder layer and epithelium was reconstituted on the dermal equivalents consisting of fibroblast and t y p e I collagen. After growing under an air-liquid surface for 2 weeks, morphologic and biologic characteristics were evaluated. We also assessed the effect of type IV collagen and laminin on the dermal equivalent during the epithelial reconstitution. Isografted reconstituted oral epithelium on nude mice(balb/c) were evaluated histologically and immunohistochemically. Epithelia on the dermal equivalent showed multilayered stratified squamous epithelium which has a reverse polarity. Each of type IV collagen and laminin induce dyskeratotic cells and protrusion int~o the collagen. Molar-ratio mixtures of both matrices induce the well-organized epithelium to show hemidesmosomes and basal lamina as well as similar immunohistochemical characteristics to in vivo. Grafted tissue on the nude mice show similar morphologic and biologic characteristics. These results suggest that reconstitute epithelium with type IV collagen and laminin might be a useful for epithelial graft and experimental model. [This work was supported by Korea Science and Engineering Foundation (981-0707-064-1).]
cal study by measuring the regional thickness of the parietal bone on 30 Korean adult dry skulls. Before the sectioning the calvarium the anatomical landmarks were marked on each specimens. And then we measured the total thickness of the parietal bone and the thickness of the outer and inner cortical plates on various points in each sections of parietal bones using a digital caliper under the stereomicroscope. The total thickness of the parietal bone was ranged from 5.1 mm to 7.5 mm, and there was no statistical difference in the total thickness of the parietal bone on the same points bilaterally. But there was a tendency that the thickness of the parietal bone was thicker toward to the lambda point than the coronal suture area. At the other hand, the thickness of outer and inner plate of the parietal bone was the thickest at the first point of the right aspect on the line 1, the first point of the left aspect on the line 5, respectively. In conclusion, this study showed that the donor site of the parietal bone for the maxillofacial reconstruction should be located -at more posterior and medial area of parietal bone than the prevalent donor site.
6. The Value of Computer-Aided Planning and Intraoperative Navigation in Orbital Reconstruction
Gellrich, N.., Schramm, A., Hammer, B., Schmelzeisen, R. Department of Oral and Maxillofacial Surgery, University of Freiburg, Freiburg, Germany Subject Reconstruction of posttraumatic orbital and periorbital deformities are a surgical challenge. The predictive and intraoperative advantage concerning postsurgical outcome by using modern navigation systems is investigated in 7 (peri-) orbital posttraumatic defects and an optic nerve decompression at the optic canal. Method Spiral computed tomography data of patients were acquired with Siemens Somatom Plus4 and transferred to STN-Navigation-System (Leibingeri/Zeiss). Periorbital bony and soft tissue contours, orbital contents, angles and distances were measured preoperatively on the workstation in a multiplanar view. Furthermore the reconstruction was virtually designed by mirroring the unaffected side to the deformed side. Intraoperatively, contours of preoperative planning were navigated by the surgeon using the STN workstation together with infrared cameras.
5. Regional Thickness of the Parietal Bone in Korean Adults
Choi, S. a, Cha, L 1, Kim, H), Jeong, E 1, Yi, C ~, Jeong, 1.3 1Department of Oral and Maxillofacial Surgery, 2Department of Oral Biology, College of Dentistry, 3Department of Anatomy, College of Medicine, Yonsei University To clarify the clinical utility of the calavarial bone graft in the maxillofacial reconstruction, we performed an anatomi-
Results In 7 cases of orbital reconstruction (enophthalmus correction, malar bone augmentation) surgical steps could be performed precisely as preoperatively planned on the workstation. Autologous bone could be harvested and molded to the demands of the surgeon to cover or augment defects of the orbit and periorbital region. In posttraumatic optic nerve decompression at the optic canal the surgical route could be exactly planned and the surgeon could be securely guided to the region of interest i.e. the lateral wall of the sphenoid sinus.