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S205 TYPE II COLLAGENASE INTRA-ARTICULAR INJECTION AS A MODEL OF NOCICEPTION IN OSTEOARTHRITIS IN RATS
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POSTER SESSIONS / European Journal of Pain Supplements 5 (2011) 15–295
p = 3×10−8 (OR: 1.2, 95%CI: 1.14–1.32). The top-variant is located in the 5p15.2-region, near CCT5 (Chaperonin-Containing-TCP1complex-5), a protein expressed in brain. Interestingly, Bouhouche et al. (2006) reported one family in which a CCT mutation caused “hereditary sensory neuropathy”. The other ten signals did not replicate. Conclusions: We found evidence for a common genetic variant on chromosome 5p15.2, located near CCT5, to be involved in CWP. Further studies on the function of this gene are ongoing. Disclosure: None declared
S203 THE EFFECTIVENESS OF BOTOX® INJECTION FOR THE TREATMENT OF INGUINAL PAIN Z. Elchami *, O. Asali, J. Akiki. Pain and Headcahe Management COE, IMC, Jeddah, Saudi Arabia Background and Aims: Common causes of inguinal pain are activities in which forceful adduction of the hips happen, such as inguinal pull or strain of the inner thigh muscles. Inguinal pulls are common in sports like football, soccer, baseball and hockey. The objective of the study is to evaluate the effectiveness of Botox® injection, applied to the inguinal muscles region, in the management of inguinal pain. Methods: 6 patients, all footballers, were evaluated at the Pain & Headache Center, IMC, KSA. Patients were allocated to receive either muscle relaxant medications, Cyclobenzaprine 10 mg, daily, or treated with 100 units of Botox® , injected once, in the following muscles: iliopsoas, sartorius, pectineus, adductor longus, with 25units injected in each. Patients were followed up to 2 years period. Inclusive criteria: 6 male patients; ages between 20–40 y/o, with mean of 30. Exclusive criteria: pediatric patients; patients older than 50; with uncontrolled diabetes, blood pressure; and other neurological deficits. Results: Average improvement of 70%, according to the numeric pain scale, was seen in patients within 4 weeks after the injection and the effect lasted for more than 18 months. All patients followed a schedule of good Physiotherapy sessions 15 days after the injection, and were able to go back to their normal sports activities gradually. However, patients on muscle relaxants continued to have pain in spite of treatment and Physiotherapy. Only partial improvement was appreciated. Conclusions: Patient who received Botox® injection showed more rapid, significant and sustained improvement compared to patients who received muscle relaxant medications. Disclosure: None declared
S204 MIA-INDUCED OSTEOARTHRITIS SHOWS DOSE-DEPENDENT EXPRESSION OF NEURONAL INJURY MARKERS J. Ferreira-Gomes1,2 , S. Ad˜aes1,2 *, R. Sousa1,2 , M. Mendonca ¸ 1,2 , J.M. Castro-Lopes1,2 . 1 Department of Experimental Biology, Faculty of Medicine of the University of Porto, 2 Institute of Molecular and Cell Biology (IBMC), University of Porto, Porto, Portugal Background and Aims: Changes in neurons innervating rat knee joints, indicative of neuronal damage, have been reported in the mono-iodoacetate (MIA) experimental model of osteoarthritis (OA). Therefore, we evaluated the expression of the neuronal injury markers, ATF-3 and NPY, in primary afferent neurons of OA animals injected with three different doses of MIA. Methods: All procedures were performed according to the ethical guidelines for the study of experimental pain in conscious animals. OA was induced by injection of 0.3, 1 or 2 mg of mono-iodoacetate in the knee joint of rats. Animals were sacrificed at 3, 7, 14, 21 and 31 days post-injection. The histopathology of the joints and the nociceptive behaviour were analysed. L3-L5 DRGs were used for immunohistochemistry for ATF-3 and NPY.
Results: OA animals showed the characteristic histopathological changes of the joints and the accompanying nociceptive behaviour. An increase in the number of ATF-3-positive cells was observed as early as 3 days after the induction of OA for 1 or 2 mg of MIA and 7 days after the injection of 0.3 mg. NPY expression was observed in animals injected with 1 mg at day 3 and in animals injected with 2 mg in all time-points. Conclusions: The increased ATF-3 and NPY expression suggests occurrence of damage to DRG neurons in the MIA model of OA. Since these alterations are observed at early time-points, when no histopathological evidence of disease exists, we might be in the presence of a chemically-induced neuropathy caused by MIA itself. Disclosure: None declared
S205 TYPE II COLLAGENASE INTRA-ARTICULAR INJECTION AS A MODEL OF NOCICEPTION IN OSTEOARTHRITIS IN RATS S. Ad˜aes1,2 *, J. Ferreira-Gomes1,2 , M. Mendonca ¸ 1,2 , F.L. Neto1,2 , J.M. Castro-Lopes1,2 . 1 Department of Experimental Biology, Faculty of Medicine of the University of Porto, 2 Institute of Molecular and Cell Biology (IBMC), University of Porto, Porto, Portugal Background and Aims: In the mono-iodoacetate (MIA) model of osteoarthritis, we recently observed expression of the neuronal injury markers ATF-3 and NPY as early as 3 days post-injection. Correlation with histopathological data points to a direct neurotoxic action of MIA, compromising its application as a suitable inducer to study OA. Therefore, an alternative model is necessary. Intraarticular injection of type II collagenase (ColII) has been used in the study of OA, although there are few evidences of its applicability for nociception studies. The aim of this work is to evaluate whether ColII injection into the knee of rats can be an alternative and clinically relevant model to study OA-associated pain. Methods: OA was induced by double injection of 250, 500 or 750U of ColII in the knee joint of rats, at days 0 and 3. Movementinduced nociception was evaluated by the Knee-Bend and CatWalk tests. Animals were sacrificed 1, 2, 4 and 6 weeks after the first ColII injection. L3-L5 DRGs were used for immunohistochemistry for ATF-3 and NPY and knee joints used for histopathological evaluation. Results: All doses induced an increase in nociceptive behaviour and histopathological changes, at 4 and 6 weeks, similar to those observed in clinical OA. At early time-points ATF-3 and NPY expression remained unaltered, for the lower doses. At 4 and 6 weeks, when the subchondral bone is exposed, there was increased expression of both neuronal injury markers. Conclusions: CollII injection may be a clinically relevant alternative model for the study of nociception in OA. Disclosure: None declared
S206 DIFFERENT PATTERNS OF NEEDLE ELECTROMYOGRAPHY EVALUATION OF TRIGGER POINTS IN WOMEN WITH CHRONIC PELVIC PAIN SECONDARY TO MYOFASCIAL SYNDROME PAIN C.C.M. Chamochumbi1 *, M. Pitanguy1 , P. Toscano2 , W.M. Junior2 , J.C. Rosa-e-Silva1 , F.J. Candido-dos-Reis1 , A.A. Nogueira1 , O.B. PoliNeto3 . 1 Department of Gynecology and Obstetrics, 2 Department of Neurology, Psychiatry and Medical Psychology, 3 Department of Gynecology and Obstetrics/Surgery and Anatomy, Faculty of Medicine of Ribeir˜ ao Preto/University of S˜ ao Paulo, Ribeir˜ ao Preto, Brazil Background and Aims: Chronic pelvic pain is a highly prevalent disease and affects labor and social life of the women affected. The aim of this study was to report the different patterns of needle electromyography in the evaluation of trigger points of women with chronic pelvic pain caused by abdominal myofascial pain syndrome.
POSTER SESSIONS / European Journal of Pain Supplements 5 (2011) 15–295
p = 3×10−8 (OR: 1.2, 95%CI: 1.14–1.32). The top-variant is located in the 5p15.2-region, near CCT5 (Chaperonin-Containing-TCP1complex-5), a protein expressed in brain. Interestingly, Bouhouche et al. (2006) reported one family in which a CCT mutation caused “hereditary sensory neuropathy”. The other ten signals did not replicate. Conclusions: We found evidence for a common genetic variant on chromosome 5p15.2, located near CCT5, to be involved in CWP. Further studies on the function of this gene are ongoing. Disclosure: None declared
S203 THE EFFECTIVENESS OF BOTOX® INJECTION FOR THE TREATMENT OF INGUINAL PAIN Z. Elchami *, O. Asali, J. Akiki. Pain and Headcahe Management COE, IMC, Jeddah, Saudi Arabia Background and Aims: Common causes of inguinal pain are activities in which forceful adduction of the hips happen, such as inguinal pull or strain of the inner thigh muscles. Inguinal pulls are common in sports like football, soccer, baseball and hockey. The objective of the study is to evaluate the effectiveness of Botox® injection, applied to the inguinal muscles region, in the management of inguinal pain. Methods: 6 patients, all footballers, were evaluated at the Pain & Headache Center, IMC, KSA. Patients were allocated to receive either muscle relaxant medications, Cyclobenzaprine 10 mg, daily, or treated with 100 units of Botox® , injected once, in the following muscles: iliopsoas, sartorius, pectineus, adductor longus, with 25units injected in each. Patients were followed up to 2 years period. Inclusive criteria: 6 male patients; ages between 20–40 y/o, with mean of 30. Exclusive criteria: pediatric patients; patients older than 50; with uncontrolled diabetes, blood pressure; and other neurological deficits. Results: Average improvement of 70%, according to the numeric pain scale, was seen in patients within 4 weeks after the injection and the effect lasted for more than 18 months. All patients followed a schedule of good Physiotherapy sessions 15 days after the injection, and were able to go back to their normal sports activities gradually. However, patients on muscle relaxants continued to have pain in spite of treatment and Physiotherapy. Only partial improvement was appreciated. Conclusions: Patient who received Botox® injection showed more rapid, significant and sustained improvement compared to patients who received muscle relaxant medications. Disclosure: None declared
S204 MIA-INDUCED OSTEOARTHRITIS SHOWS DOSE-DEPENDENT EXPRESSION OF NEURONAL INJURY MARKERS J. Ferreira-Gomes1,2 , S. Ad˜aes1,2 *, R. Sousa1,2 , M. Mendonca ¸ 1,2 , J.M. Castro-Lopes1,2 . 1 Department of Experimental Biology, Faculty of Medicine of the University of Porto, 2 Institute of Molecular and Cell Biology (IBMC), University of Porto, Porto, Portugal Background and Aims: Changes in neurons innervating rat knee joints, indicative of neuronal damage, have been reported in the mono-iodoacetate (MIA) experimental model of osteoarthritis (OA). Therefore, we evaluated the expression of the neuronal injury markers, ATF-3 and NPY, in primary afferent neurons of OA animals injected with three different doses of MIA. Methods: All procedures were performed according to the ethical guidelines for the study of experimental pain in conscious animals. OA was induced by injection of 0.3, 1 or 2 mg of mono-iodoacetate in the knee joint of rats. Animals were sacrificed at 3, 7, 14, 21 and 31 days post-injection. The histopathology of the joints and the nociceptive behaviour were analysed. L3-L5 DRGs were used for immunohistochemistry for ATF-3 and NPY.
Results: OA animals showed the characteristic histopathological changes of the joints and the accompanying nociceptive behaviour. An increase in the number of ATF-3-positive cells was observed as early as 3 days after the induction of OA for 1 or 2 mg of MIA and 7 days after the injection of 0.3 mg. NPY expression was observed in animals injected with 1 mg at day 3 and in animals injected with 2 mg in all time-points. Conclusions: The increased ATF-3 and NPY expression suggests occurrence of damage to DRG neurons in the MIA model of OA. Since these alterations are observed at early time-points, when no histopathological evidence of disease exists, we might be in the presence of a chemically-induced neuropathy caused by MIA itself. Disclosure: None declared
S205 TYPE II COLLAGENASE INTRA-ARTICULAR INJECTION AS A MODEL OF NOCICEPTION IN OSTEOARTHRITIS IN RATS S. Ad˜aes1,2 *, J. Ferreira-Gomes1,2 , M. Mendonca ¸ 1,2 , F.L. Neto1,2 , J.M. Castro-Lopes1,2 . 1 Department of Experimental Biology, Faculty of Medicine of the University of Porto, 2 Institute of Molecular and Cell Biology (IBMC), University of Porto, Porto, Portugal Background and Aims: In the mono-iodoacetate (MIA) model of osteoarthritis, we recently observed expression of the neuronal injury markers ATF-3 and NPY as early as 3 days post-injection. Correlation with histopathological data points to a direct neurotoxic action of MIA, compromising its application as a suitable inducer to study OA. Therefore, an alternative model is necessary. Intraarticular injection of type II collagenase (ColII) has been used in the study of OA, although there are few evidences of its applicability for nociception studies. The aim of this work is to evaluate whether ColII injection into the knee of rats can be an alternative and clinically relevant model to study OA-associated pain. Methods: OA was induced by double injection of 250, 500 or 750U of ColII in the knee joint of rats, at days 0 and 3. Movementinduced nociception was evaluated by the Knee-Bend and CatWalk tests. Animals were sacrificed 1, 2, 4 and 6 weeks after the first ColII injection. L3-L5 DRGs were used for immunohistochemistry for ATF-3 and NPY and knee joints used for histopathological evaluation. Results: All doses induced an increase in nociceptive behaviour and histopathological changes, at 4 and 6 weeks, similar to those observed in clinical OA. At early time-points ATF-3 and NPY expression remained unaltered, for the lower doses. At 4 and 6 weeks, when the subchondral bone is exposed, there was increased expression of both neuronal injury markers. Conclusions: CollII injection may be a clinically relevant alternative model for the study of nociception in OA. Disclosure: None declared
S206 DIFFERENT PATTERNS OF NEEDLE ELECTROMYOGRAPHY EVALUATION OF TRIGGER POINTS IN WOMEN WITH CHRONIC PELVIC PAIN SECONDARY TO MYOFASCIAL SYNDROME PAIN C.C.M. Chamochumbi1 *, M. Pitanguy1 , P. Toscano2 , W.M. Junior2 , J.C. Rosa-e-Silva1 , F.J. Candido-dos-Reis1 , A.A. Nogueira1 , O.B. PoliNeto3 . 1 Department of Gynecology and Obstetrics, 2 Department of Neurology, Psychiatry and Medical Psychology, 3 Department of Gynecology and Obstetrics/Surgery and Anatomy, Faculty of Medicine of Ribeir˜ ao Preto/University of S˜ ao Paulo, Ribeir˜ ao Preto, Brazil Background and Aims: Chronic pelvic pain is a highly prevalent disease and affects labor and social life of the women affected. The aim of this study was to report the different patterns of needle electromyography in the evaluation of trigger points of women with chronic pelvic pain caused by abdominal myofascial pain syndrome.