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Sicilian olive oil antioxidants and prevention of cardiovascular diseases
XVIII S.I.S.A. National Congress
302
THE METABOLIC SYNDROME IN THE ELDERLY SUBJECTS OF THE PROGETTO VENETO ANZIANI (Pro.V.A.) STUDY
Antibodies against modified LDL inversely correlate with carotid artery IMT in a healthy population of the northern area of Milan.
Romanato G, Zambon S, Manzato E, Corti MC, Baggio G, Marin R, Zanoni S, Sartori L, Tezza F, Crepaldi G
1,2 F.M.Maggi, 1S.Raselli ,1S.Fantappi~,lL Grigore,lE. Sambugaro,1 K.Garlaschelli, 1L.Redaelli and 1,2A.L.Catapano
Department of Medical and Surgical Sciences, University of Padova, Italy
University of Milan: 1Atherosclerosis Centre, Bassini Hospital , 2 Department of Pharmacological Sciences.
The Pro.V.A Study is an observational cohort study of an italian population aged 65 or more years residing in Northeast of Italy. The study population included 3099 age- and sex-stratified subjects randomly selected from health district list. Participants were interviewed and medically examined. According to the NCEP-ATP III criteria the prevalence of metabolic syndrome (MS) in the Pro.V.A. population is 22% in men (M) and 43% in women (W). The major determinants for MS diagnosis in our older population are high blood pressure in both sexes (present in 73% of M and 75% of W), and the waist circumference in W (larger than 88 cm in 73% of W). Age, educational level, smoking and alcohol consumption are no different in M and W with or without MS. Both M and W with MS show higher glycated hemoglobin and uric acid than subjects without MS. Only M with MS have higher plasma and LDL cholesterol levels than M without. Both M and W with MS have higher levels of ALT, GGT, ESR and leukocytes compared to subjects without. Only W with MS show higher fibrinogen levels. Subjects with MS show a prevalence of any artery disease (coronary, cerebrovascular and peripheral artery diseases) significantly higher than subjects without MS: 38% in M with MS vs 26% in M without, 21% in W with MS vs 16% in W without. Our old population shows a high prevalence of MS, particularly in the W, due to the high percentage of obese W of the Pro.V.A. Study.
SICILIAN OLIVE OIL ANTIOXlDANTS AND PREVENTION OF CARDIOVASCULAR DISEASES E. Tripoli, S. Giuliano *, D. Di Majo, M. Giammanco, P. Gagliano, G. Tabacchi, S. Giammanco, M. La Guardia Istituto di Fisiologia e Nutrizione Umana Universit~ di Palermo*Agenzia delie Dogane Introduction:Epidemiological studies have shown a lower incidence of atherosclerosis, cardiovascular diseases and cancer in the Mediterranean area. Olive oil, the main source of fat in the Mediterranean diet, has healthy effects attributed not only to the high relationship between unsaturated/saturated fatty acids but also to the anti-oxidant property of its phenolic compounds. Sicily produces extra-virgin olive oil of good quality.Aim: Since the amount of phenolic compounds in olive oil depends on several factors such as cultivar, degree of maturation, production process, we estimate the Sicilian cultivar that containing the highest amount of polyphenolic compounds. Methods:We have analysed 110 samples of extra-virgin olive oil produced in the 2003. All the samples was from the different Sicilian DOP. Polyphenols were analyzed according to the Folin-Ciocalteu method. Moreover, we have estimated the oleic acidity, peroxyde number, K232, K270, delta K, total tocopherols and fatty acids.Results: the highest amounts of tocopherols and polyphenols are contained in the nocellara (for polyphenols the highest value is 507 mg/kg and for tocopherols is 409 mg/kg), while the lowest amounts are contained in oglialora (for polyphenols the highest value is 114 mg/kg and for tocopherols is 284 mg/kg).Conclusion:This study shows that the nocellara provides an olive oil richest in anti-oxidant compounds. Although all the analysed samples have excellent qualitative parameters, it can be supposed that the consumption of olive oil coming from the DOP Nocellara could contribute in a best way to the prevention of the cardiovascular diseases.(annullo precedente, esprimo preferenza per il Poster)
We investigated the relations between antibody titers against MDA-LDL and IMT (Intima Media Thickness) of the common carotid artery in a healthy population of the northern area of Milan. Through general practitioners of the northern area of Milan, more than 2000 people were involved in the PLIC study. Biochemical analysis, demographic variables and IMT were assessed for each subject. We excluded from total data base subjects with CVD, liver or kidney diseases or thyroid dysfunction, and on hypolipidemic or antihypertensive therapies (n=1767). In a randomly selected subpopulation (n=176) we evaluate autoantibody titers against MDA-LDL, detected by a specific immuno-assay. The characteristics of the entire population and the selected group were comparable. Antibody titers showed a skewed distribution, therefore their values were log. transformed for correlation with normally distributed parameters. At a bivariate analysis IMT showed an inverse correlation withy antibody titers, both IgG and IgM (p<0.0001 and p<0.01 respectively). IgG titers, after age and triglyceride plasma levels, are independently related to IMT (step wise regression analysis with IMT as dependent variable and age, BMI, glucose, lipid parameters and log of antibody titers as independent variables). We can speculate that, in a healthy population, immune response against modified LDL may be protective towards the progression of carotid atherosclerosis.
Dietary habits in a population in the north area of Milan: the PLIC study 1LRedaelli, 1L Grigore, 1K.Garlaschelli, 1,2 F.M..Maggi, 1S.Raselli, 1S.Fantappi~ and 1,2A.L.Catapano University of Milan : 1Atherosclerosis Center, Bassini Hospital, 2Department of Pharmacological Sciences A lower incidence of cardiovascular diseases (CVD) is present in Mediterranean populations in respect to other European areas and United States. Dietary intervention is a cornerstone in the prevention of CVD. Population: 372 subjects (221 women and 149 men) were randomly selected from a cohort of 1846 healthy individuals (40,8% men and 59,2% women) recruited in the PLIC Study. Mean+SE age was 54,0+0.7 years, BMI 26,6+0.2 Kg/m2, LDL-C 146:1:2 mg/dL, HDL-C 56+1 mg/dL, triglycerides 111+4 mg/dL, glucose 94+1 mg/dL. Framingham cardiovascular risk (RCV) was 13.4+1%. Methods: seven-day dietary recall forms were collected to evaluate the mean % of total kcal for proteins, lipids and carbohydrates/day, the amounts of saturated and unsaturated fatty acids. Objective: report alimentary habits in the studied population. Results: the daily intake was 1639+21 Kcal, 16% of proteins, 48% of carbohydrates and 36% lipids, with 10.8% of saturated fatty acids, 16.5% of mono and 4.5% of poll-unsaturated fatty acids. Dietary cholesterol intake was 216+5 mg/day. Kcal intake in women was lower than in men (1556+23 Kcal/die vs. 1759:1:36 Kcal/die, p<0.001), as well as cholesterol intake (205+5 mg/die vs. 232+9 mg/die, p=0.014), whereas the % of kcal for saturated fatty acids were higher in women (11.1+0.2% vs. 10.4+0.2%, p=0.02). 88.2% of the population (n=328) has a lipid intake of >30% of the total daily kcal. A significant correlation between lipid intake and RCV (Framingham, R=0.168, p=0.013)was present in this group. Conclusions: In the population studied, the lipid and carbohydrates intake is higher than the European recommendations. Lipid intake was associated to cardiovascular risk.
302
THE METABOLIC SYNDROME IN THE ELDERLY SUBJECTS OF THE PROGETTO VENETO ANZIANI (Pro.V.A.) STUDY
Antibodies against modified LDL inversely correlate with carotid artery IMT in a healthy population of the northern area of Milan.
Romanato G, Zambon S, Manzato E, Corti MC, Baggio G, Marin R, Zanoni S, Sartori L, Tezza F, Crepaldi G
1,2 F.M.Maggi, 1S.Raselli ,1S.Fantappi~,lL Grigore,lE. Sambugaro,1 K.Garlaschelli, 1L.Redaelli and 1,2A.L.Catapano
Department of Medical and Surgical Sciences, University of Padova, Italy
University of Milan: 1Atherosclerosis Centre, Bassini Hospital , 2 Department of Pharmacological Sciences.
The Pro.V.A Study is an observational cohort study of an italian population aged 65 or more years residing in Northeast of Italy. The study population included 3099 age- and sex-stratified subjects randomly selected from health district list. Participants were interviewed and medically examined. According to the NCEP-ATP III criteria the prevalence of metabolic syndrome (MS) in the Pro.V.A. population is 22% in men (M) and 43% in women (W). The major determinants for MS diagnosis in our older population are high blood pressure in both sexes (present in 73% of M and 75% of W), and the waist circumference in W (larger than 88 cm in 73% of W). Age, educational level, smoking and alcohol consumption are no different in M and W with or without MS. Both M and W with MS show higher glycated hemoglobin and uric acid than subjects without MS. Only M with MS have higher plasma and LDL cholesterol levels than M without. Both M and W with MS have higher levels of ALT, GGT, ESR and leukocytes compared to subjects without. Only W with MS show higher fibrinogen levels. Subjects with MS show a prevalence of any artery disease (coronary, cerebrovascular and peripheral artery diseases) significantly higher than subjects without MS: 38% in M with MS vs 26% in M without, 21% in W with MS vs 16% in W without. Our old population shows a high prevalence of MS, particularly in the W, due to the high percentage of obese W of the Pro.V.A. Study.
SICILIAN OLIVE OIL ANTIOXlDANTS AND PREVENTION OF CARDIOVASCULAR DISEASES E. Tripoli, S. Giuliano *, D. Di Majo, M. Giammanco, P. Gagliano, G. Tabacchi, S. Giammanco, M. La Guardia Istituto di Fisiologia e Nutrizione Umana Universit~ di Palermo*Agenzia delie Dogane Introduction:Epidemiological studies have shown a lower incidence of atherosclerosis, cardiovascular diseases and cancer in the Mediterranean area. Olive oil, the main source of fat in the Mediterranean diet, has healthy effects attributed not only to the high relationship between unsaturated/saturated fatty acids but also to the anti-oxidant property of its phenolic compounds. Sicily produces extra-virgin olive oil of good quality.Aim: Since the amount of phenolic compounds in olive oil depends on several factors such as cultivar, degree of maturation, production process, we estimate the Sicilian cultivar that containing the highest amount of polyphenolic compounds. Methods:We have analysed 110 samples of extra-virgin olive oil produced in the 2003. All the samples was from the different Sicilian DOP. Polyphenols were analyzed according to the Folin-Ciocalteu method. Moreover, we have estimated the oleic acidity, peroxyde number, K232, K270, delta K, total tocopherols and fatty acids.Results: the highest amounts of tocopherols and polyphenols are contained in the nocellara (for polyphenols the highest value is 507 mg/kg and for tocopherols is 409 mg/kg), while the lowest amounts are contained in oglialora (for polyphenols the highest value is 114 mg/kg and for tocopherols is 284 mg/kg).Conclusion:This study shows that the nocellara provides an olive oil richest in anti-oxidant compounds. Although all the analysed samples have excellent qualitative parameters, it can be supposed that the consumption of olive oil coming from the DOP Nocellara could contribute in a best way to the prevention of the cardiovascular diseases.(annullo precedente, esprimo preferenza per il Poster)
We investigated the relations between antibody titers against MDA-LDL and IMT (Intima Media Thickness) of the common carotid artery in a healthy population of the northern area of Milan. Through general practitioners of the northern area of Milan, more than 2000 people were involved in the PLIC study. Biochemical analysis, demographic variables and IMT were assessed for each subject. We excluded from total data base subjects with CVD, liver or kidney diseases or thyroid dysfunction, and on hypolipidemic or antihypertensive therapies (n=1767). In a randomly selected subpopulation (n=176) we evaluate autoantibody titers against MDA-LDL, detected by a specific immuno-assay. The characteristics of the entire population and the selected group were comparable. Antibody titers showed a skewed distribution, therefore their values were log. transformed for correlation with normally distributed parameters. At a bivariate analysis IMT showed an inverse correlation withy antibody titers, both IgG and IgM (p<0.0001 and p<0.01 respectively). IgG titers, after age and triglyceride plasma levels, are independently related to IMT (step wise regression analysis with IMT as dependent variable and age, BMI, glucose, lipid parameters and log of antibody titers as independent variables). We can speculate that, in a healthy population, immune response against modified LDL may be protective towards the progression of carotid atherosclerosis.
Dietary habits in a population in the north area of Milan: the PLIC study 1LRedaelli, 1L Grigore, 1K.Garlaschelli, 1,2 F.M..Maggi, 1S.Raselli, 1S.Fantappi~ and 1,2A.L.Catapano University of Milan : 1Atherosclerosis Center, Bassini Hospital, 2Department of Pharmacological Sciences A lower incidence of cardiovascular diseases (CVD) is present in Mediterranean populations in respect to other European areas and United States. Dietary intervention is a cornerstone in the prevention of CVD. Population: 372 subjects (221 women and 149 men) were randomly selected from a cohort of 1846 healthy individuals (40,8% men and 59,2% women) recruited in the PLIC Study. Mean+SE age was 54,0+0.7 years, BMI 26,6+0.2 Kg/m2, LDL-C 146:1:2 mg/dL, HDL-C 56+1 mg/dL, triglycerides 111+4 mg/dL, glucose 94+1 mg/dL. Framingham cardiovascular risk (RCV) was 13.4+1%. Methods: seven-day dietary recall forms were collected to evaluate the mean % of total kcal for proteins, lipids and carbohydrates/day, the amounts of saturated and unsaturated fatty acids. Objective: report alimentary habits in the studied population. Results: the daily intake was 1639+21 Kcal, 16% of proteins, 48% of carbohydrates and 36% lipids, with 10.8% of saturated fatty acids, 16.5% of mono and 4.5% of poll-unsaturated fatty acids. Dietary cholesterol intake was 216+5 mg/day. Kcal intake in women was lower than in men (1556+23 Kcal/die vs. 1759:1:36 Kcal/die, p<0.001), as well as cholesterol intake (205+5 mg/die vs. 232+9 mg/die, p=0.014), whereas the % of kcal for saturated fatty acids were higher in women (11.1+0.2% vs. 10.4+0.2%, p=0.02). 88.2% of the population (n=328) has a lipid intake of >30% of the total daily kcal. A significant correlation between lipid intake and RCV (Framingham, R=0.168, p=0.013)was present in this group. Conclusions: In the population studied, the lipid and carbohydrates intake is higher than the European recommendations. Lipid intake was associated to cardiovascular risk.