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Sinusitis subsequent to fractures of orbital-zygomaticomaxillary complex
e172
Oral Presentation
development of malignant tumors such as basal cell carcinoma and medulloblastoma. SHH is a secreted protein that activates a membrane-receptor complex formed by patched (PTCH) and smoothened (SMO). In the present research, we demonstrated a necessary role in the growth SHH signaling pathway of tumor in the same way in the ameloblastoma, and it was found that suppression of proliferation by its inhibition was possible. GDC-0449 is a hedgehog pathway inhibitor that binds and inhibits SMO, a transmembrane protein involved in the hedgehog signaling transduction. Cyclopamine is the prototype inhibitor of the SHH pathway that inactivates SMO binding to its hepta-helical bundle. Research objectives: In this study, we investigated the effects of the potent SMO inhibitors of sonic hedgehog (SHH) on ameloblastoma cell lines. Materials and methods: The human ameloblastoma cell line AM-1, was established from human plexiform type ameloblastoma tissue and immortalized by the transfection of human papillomavirus type 16 DNA. The normal oral mucosal epithelial immortalized cell line, MOE-1 (mutation of CDK4, cyclinD1, TERT (human telomerase reverse transcriptase) and dominant negative form of p53 gene primary culture cells). We examined the expression of HH signaling pathway genes (SHH, PTCH, SMO, GLI1, GLI2, GLI3) and proteins in each cell line. We focused on the impact that each HH signaling pathway inhibitors have on growth in each cell line by WST-8 cell proliferation assay and BrdU uptake assay. We detected the apoptotic cells by Annexin-V fluos conjugated with propidium iodide which added to the GDC-0449 or cyclopamine group. Results: The hedgehog signaling inhibitors, cyclopamine, and GDC-0449 were used to assess the effect of inhibitor on AM-1 cell lines. RT-PCR analysis revealed that SHH, PTCH, SMO, GLI1, GLI2, and GLI3 were expressed in the AM-1, AM-3 and MOE1b cells. Expression of SHH-related genes and gene products in the AM-1, AM-3 and MOE-1b cells was mainly expressed by immunocytochemistry. The addition of GDC-0449 or cyclopamine suppressed proliferation of AM-1 cell lines. In the presence of GDC-0449 or cyclopamine, it was inhibited the growth of ameloblastoma cell lines. Annexin-V positive cells are significantly more common in the presence of GDC-0449 or cyclopamine than its absence. Conclusions: Our study suggests to develop a new treatment of ameloblastoma by growth inhibition and apoptosis induction by SHH signaling pathway inhibitor such as GDC-0449 or cyclopamine. http://dx.doi.org/10.1016/j.ijom.2015.08.888 Relationship in orbital trauma between time of reconstruction surgery and diplopia C. Pedemonte ∗ , E. Gonzalez, I. Vargas, E. Navia, K. Salazar, M. Canales, F. Saez, C. Zamora Hospital Clínico Mutual de Seguridad C.CH.C., Santiago, Chile In general, trauma centers have limitations regarding the availability of staff, physical resources and physical condition for the treatment of patient that determine the intervention time of a fracture. Even though, it is well described that in orbital trauma the best time for treatment is an immediate reduction, it is not always possible and most of the time is performed on a delayed time. In the case of orbital trauma there is the possibility of diplopia as a
sequel in relationship of time of reconstruction. We analyzed clinical record during the years 2011 and 2012 in the Hospital Clinico Mutual de Seguridad – Santiago, Chile, searching for a relationship between the time from trauma and reconstruction surgery of the orbit and quantifying how many of them reported diplopia as a permanent sequelae. http://dx.doi.org/10.1016/j.ijom.2015.08.889 Sinusitis subsequent to zygomaticomaxillary complex
fractures
of
orbital-
I. Vargas, E. Gonzalez, C. Pedemonte, E. Navia, C. Zamora ∗ , F. Saez, K. Salazar, M. Canales Hospital Clínico Mutual de Seguridad C.CH.C., Santiago, Chile Facial traumas that generate orbital-zygomaticomaxillary complex fractures mostly involved the maxillary sinus. In order to analyze the statistics and causes of sinus complications associated with trauma and reduction of this area, we performed a retrospective study in patients from the maxillofacial surgery service of Hospital Clinico Mutual de Seguridad. 596 patients underwent open reduction and osteosynthesis of their orbital zygomatic fracture during the years 2010–2011. Those patients requiring treatment due to maxillary sinus complication were followed by two consecutive years (2012–2013), and derived to otolaryngology. In all cases we analyzed the reason of the complication. Overall, the study obtained a low incidence of sinus complications (6.91%) which were primarily associated with the persistence of fine bone fragments, due poor irrigation or comminuted fractures. http://dx.doi.org/10.1016/j.ijom.2015.08.890 Squamous cell carcinoma of the oral cavity derived from a skin graft in a previous SCC site: a case report P. Zarringhalam ∗ , C. Leigh, A.V. Parbhoo, C.H. Chan Luton and Dunstable NHS Foundation Trust, Bedfordshire, Hertfordshire and Buckinghamshire OMFS Network, London, UK Background: Surgical excision and reconstruction forms the mainstay of treatment for intra-oral squamous cell carcinoma (SCC). Recurrent or new primary disease can occur locally, regionally or in distant sites. Forming a new primary SCC on transferred skin is rare. It may be induced by exposure to the new environment, existing skin tumor, epidermis metaplasia or graft colonization by residual tumor cells from adjacent mucosa. 4–38 years of latency has been reported for the development of SCC in skin flaps. Objectives: We present an unusual SCC derived from a 7-year old full thickness skin graft (FTSG) previously used to reconstruct an SCC excision site. We aim to highlight the importance of long term monitoring. Case/method: A 74 year old male patient who initially underwent local excision and a FTSG reconstruction of an SCC of the left buccal mucosa, developed lichen planus of the FTSG 7 years later, followed by a new primary SCC. Further local excision and a second FTSG alongside a selective neck dissection and adjuvant radiotherapy was performed. Findings: We reveal that new primary SCC can occur in the same anatomical site as a previous SCC and resemble recurrence. It is difficult to determine if the cancer is arising from the original affected mucosa or the FTSG. We highlight the importance of
Oral Presentation
development of malignant tumors such as basal cell carcinoma and medulloblastoma. SHH is a secreted protein that activates a membrane-receptor complex formed by patched (PTCH) and smoothened (SMO). In the present research, we demonstrated a necessary role in the growth SHH signaling pathway of tumor in the same way in the ameloblastoma, and it was found that suppression of proliferation by its inhibition was possible. GDC-0449 is a hedgehog pathway inhibitor that binds and inhibits SMO, a transmembrane protein involved in the hedgehog signaling transduction. Cyclopamine is the prototype inhibitor of the SHH pathway that inactivates SMO binding to its hepta-helical bundle. Research objectives: In this study, we investigated the effects of the potent SMO inhibitors of sonic hedgehog (SHH) on ameloblastoma cell lines. Materials and methods: The human ameloblastoma cell line AM-1, was established from human plexiform type ameloblastoma tissue and immortalized by the transfection of human papillomavirus type 16 DNA. The normal oral mucosal epithelial immortalized cell line, MOE-1 (mutation of CDK4, cyclinD1, TERT (human telomerase reverse transcriptase) and dominant negative form of p53 gene primary culture cells). We examined the expression of HH signaling pathway genes (SHH, PTCH, SMO, GLI1, GLI2, GLI3) and proteins in each cell line. We focused on the impact that each HH signaling pathway inhibitors have on growth in each cell line by WST-8 cell proliferation assay and BrdU uptake assay. We detected the apoptotic cells by Annexin-V fluos conjugated with propidium iodide which added to the GDC-0449 or cyclopamine group. Results: The hedgehog signaling inhibitors, cyclopamine, and GDC-0449 were used to assess the effect of inhibitor on AM-1 cell lines. RT-PCR analysis revealed that SHH, PTCH, SMO, GLI1, GLI2, and GLI3 were expressed in the AM-1, AM-3 and MOE1b cells. Expression of SHH-related genes and gene products in the AM-1, AM-3 and MOE-1b cells was mainly expressed by immunocytochemistry. The addition of GDC-0449 or cyclopamine suppressed proliferation of AM-1 cell lines. In the presence of GDC-0449 or cyclopamine, it was inhibited the growth of ameloblastoma cell lines. Annexin-V positive cells are significantly more common in the presence of GDC-0449 or cyclopamine than its absence. Conclusions: Our study suggests to develop a new treatment of ameloblastoma by growth inhibition and apoptosis induction by SHH signaling pathway inhibitor such as GDC-0449 or cyclopamine. http://dx.doi.org/10.1016/j.ijom.2015.08.888 Relationship in orbital trauma between time of reconstruction surgery and diplopia C. Pedemonte ∗ , E. Gonzalez, I. Vargas, E. Navia, K. Salazar, M. Canales, F. Saez, C. Zamora Hospital Clínico Mutual de Seguridad C.CH.C., Santiago, Chile In general, trauma centers have limitations regarding the availability of staff, physical resources and physical condition for the treatment of patient that determine the intervention time of a fracture. Even though, it is well described that in orbital trauma the best time for treatment is an immediate reduction, it is not always possible and most of the time is performed on a delayed time. In the case of orbital trauma there is the possibility of diplopia as a
sequel in relationship of time of reconstruction. We analyzed clinical record during the years 2011 and 2012 in the Hospital Clinico Mutual de Seguridad – Santiago, Chile, searching for a relationship between the time from trauma and reconstruction surgery of the orbit and quantifying how many of them reported diplopia as a permanent sequelae. http://dx.doi.org/10.1016/j.ijom.2015.08.889 Sinusitis subsequent to zygomaticomaxillary complex
fractures
of
orbital-
I. Vargas, E. Gonzalez, C. Pedemonte, E. Navia, C. Zamora ∗ , F. Saez, K. Salazar, M. Canales Hospital Clínico Mutual de Seguridad C.CH.C., Santiago, Chile Facial traumas that generate orbital-zygomaticomaxillary complex fractures mostly involved the maxillary sinus. In order to analyze the statistics and causes of sinus complications associated with trauma and reduction of this area, we performed a retrospective study in patients from the maxillofacial surgery service of Hospital Clinico Mutual de Seguridad. 596 patients underwent open reduction and osteosynthesis of their orbital zygomatic fracture during the years 2010–2011. Those patients requiring treatment due to maxillary sinus complication were followed by two consecutive years (2012–2013), and derived to otolaryngology. In all cases we analyzed the reason of the complication. Overall, the study obtained a low incidence of sinus complications (6.91%) which were primarily associated with the persistence of fine bone fragments, due poor irrigation or comminuted fractures. http://dx.doi.org/10.1016/j.ijom.2015.08.890 Squamous cell carcinoma of the oral cavity derived from a skin graft in a previous SCC site: a case report P. Zarringhalam ∗ , C. Leigh, A.V. Parbhoo, C.H. Chan Luton and Dunstable NHS Foundation Trust, Bedfordshire, Hertfordshire and Buckinghamshire OMFS Network, London, UK Background: Surgical excision and reconstruction forms the mainstay of treatment for intra-oral squamous cell carcinoma (SCC). Recurrent or new primary disease can occur locally, regionally or in distant sites. Forming a new primary SCC on transferred skin is rare. It may be induced by exposure to the new environment, existing skin tumor, epidermis metaplasia or graft colonization by residual tumor cells from adjacent mucosa. 4–38 years of latency has been reported for the development of SCC in skin flaps. Objectives: We present an unusual SCC derived from a 7-year old full thickness skin graft (FTSG) previously used to reconstruct an SCC excision site. We aim to highlight the importance of long term monitoring. Case/method: A 74 year old male patient who initially underwent local excision and a FTSG reconstruction of an SCC of the left buccal mucosa, developed lichen planus of the FTSG 7 years later, followed by a new primary SCC. Further local excision and a second FTSG alongside a selective neck dissection and adjuvant radiotherapy was performed. Findings: We reveal that new primary SCC can occur in the same anatomical site as a previous SCC and resemble recurrence. It is difficult to determine if the cancer is arising from the original affected mucosa or the FTSG. We highlight the importance of