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Statistical and histochemical analysis of metastatic cancer of the skin
543
544
IMMUNOGENETIC PLANTS
SHOWING
KEIKO
KURATA,
KAZUE
NISHIOKA
Department
of
Medicine,
ube
ANALYSIS
OF
FAMILIAL
ALLERGY
TO
PATCHTEST RESULTS OF FORMALDEHYDE AND IMIDAZOLIDlNYLUREA (A FORMALDEHYDE RELEASINGAGENT)
COMPOSITAE
0CC”RRENCE
MASAHIKO AND
CONTPCT
MUTO,
CHIDORI
Dermatology,
TOSHIHIKO
YAMAMOTO,
RITSUKO HAYAKAWA, YASUKOOCINO, KAYOKO ARIS AND YAENO ARIMA Division of Dermatology. Nagoya University Branch Hospital
ASAGRMI
Yamaguchi
Univ.
of
School
In the past 10 years we carried out the patch testings of formaldehyde (F) and imidazolidinyl urea (G: Germal@ 115. a formaldehyde releasing agent) on 358 patients with skin diseases. 23 (6.4 t) oul of 358 were posilive to F and 4 (1. 1 %)werepositive and 3 (0.8 X1 were doubtful positive to G. 2 reacted to both of F and G. one was it to C and i? to F and one was positive to G but negative to F. Out of 3 who were i? to G. 2 were positive and one was negative to F. 2 out of 23 positively reacted to F were also postlive and 2 were doubtful positive to G. C is the most commonly used cosmelic preservative in Europe and U.S.. but UP to date it has not been permitted to use in Japan. If Germal@ 115 is used in cosmetics as a preservative in our country, 8.7% (2/23) to 17.4%(4/23) of the sensitized with formaldehyde may develope allergic contact dermatitis due to Germala 115.
We have observed familial occurrence of contact allergy to The proband wor!ang at flower shop for compositae plants. She had devel20 years was a 47-year-old Japanese female. oped symptoms of contact allergy to the plant at 42 years of She was positive by patch testing for both the composage. itae plants and a liposoltile component extracted from the plant by TLC analysis. Her younqer unaffected brother, a45 year-old Japanese male, showed a suularly positive reaction These findings suggest that by patch testxng with the plant. genetic factors might be involved in the development of contact allergy to the compositae plants.
SUPPRESSIVEEFFECTOF ORALLY ADMINISTERED CYCLOSPORIN A 'Ill FLARE-UPREACTIONON MURINECONTACT HYPERSENSITIVITY
DELAYEDPRURITIC PAPULAR REACTION AT THE SITES OF PRICK TESTS WITH ENVIRONMENTALALLERGENS--ITSPOSSIBLE PATHOGENETIC RELATIONSHIPTO PRURIGO NODULES MISAKO TANAKA AND HACHIRO TAGAMI Department of Dermatology, Tohoku Univ.
School
of
MASARU NATSUAKI, NORIKOYANASHITA AND SEICHIROSAGANI of Dermatology. Hyogo College of MEdicme
Medicine,
Department
Sendai Observation in 19 out of 4’3 subjects at the sites of prick teats with several environmental antigens such as mosquitoes, Candida, house dust mites, and pollen antigens disclosed pruritic papules after 72 hours formation of solid, discrete, or later. These reactions were observed in patients who had prurigo nodularis or atopic dermatitis associated with pruriginous nodules regardless of the presence of immediate skin reactivity to those antigens. Histologically, the lesions of prurigo nodularis and those papules producted at prick test sites showed similar patterns of inflammation and infiltrating cells. These results suggest that intradermal inoculation of environmental allergens plays an important role in the pathogenesis of pruriginous pawle~ in patients particularly in those with atopic with gruriginoua lesions, dermatitis.
Cxlosoorm A (‘&A) is well known as one of the most efficient immunosuppressive drugs. In this study, we tested whelher or not orally admmlstcred CyA showed the suppressive effect to flare-up reaction on murine contact hypersensitivity reaction (CHR) by using Rechallenge system, our original experimental system as a model of chronic contact dermatitis. As the result, it was found that
significant
suppression
when
CyA
administered
was
of
ear
to
swelling
animals
at
was the
recognized fame
only
time
as and the suppressive erfect showed the dowdependent Imanner. Therefore, It is suggested that CyA treatment may be useful for the therapy of chronic contact dermatitis. rechallenge
547
548
AN IN VITRO STUDYON THE CROSS-REACTIONBETWEEN PIROXICAM PHGTOSENSITIVITYAND TBIOSALICYLATEHYPERSENSITIVITY
STATISTICAL AND HISTCCHEMICAL STATIC CANCER OF THE StilN
TAKESHI BARIYA*, KAZUKO KITAMURA, JUNK0 OSAWA, ZENRO IKEZAWA Yokohama City AND HIROSHI NAKAJINA Dept. of Dermatology. Univ. School of Medicine. Shiseido Research Center*.Yokohana
A FUJIOKA, Department School of
nonsteroidal well known as a Piroxicarn(PXM)is photocontact which causes antiminflamatory drug. dermatitis. Recently we revealed that there is a cross-reactionbetween photosensitivityto PXM and contact sensitivity to thimerosal(TMS)and thiosalicylate(TOS)by in viva skin test in guinea pigs. Then, we investigated lymphocyte proliferation by a this crossaa3ction test (LPT) using lymph node cells(LNCs) taken from guinea pigs sensitized with TOS. LNCs from the guinea pig sensitized with TOS showed a siginificantlyproliferative response to peritoneal exudate cells(PEC) previously photohapten-modified by treatmentwith PXM plus ultraviolet A. while LNCs cultured with PEC treated with only PXM did crossax?activity between not. From this result, the photosensitivityto PXM and contact sensitivity to TOS was confirmedby LF'T.
44 cases of metastatlc cancer of the skin for 20 years I” our department were investigated. All of and primary them were diagnosed histologically, lesions were confIrmed. According to the classlflcatlon based on primary lesion, I7 cases of the 10 cases of the breast cancer, 7 lung cancer, cases of stomach cancer, 3 cases of uterus cancer and 7 case8 of the other cancers were recognized. stomach and The number of the lung, breast, the other authors cancers was large such as report. Stomach cancer mainly examined histochemparadoxical ConA and PAS lcal ly. Alclan blue, staining were performed. Expression of EGF, EGFR, also were Investigated. The character of the studled by metastatlc cancer of the skin was these analysis.
238
T ASAI, of Medicine.
ANALYSIS
TTAKEvIUF?AANDK~WVEYAW. Kitasato Sagamihara
Dermatology,
OF
META-
Un~verslty
544
IMMUNOGENETIC PLANTS
SHOWING
KEIKO
KURATA,
KAZUE
NISHIOKA
Department
of
Medicine,
ube
ANALYSIS
OF
FAMILIAL
ALLERGY
TO
PATCHTEST RESULTS OF FORMALDEHYDE AND IMIDAZOLIDlNYLUREA (A FORMALDEHYDE RELEASINGAGENT)
COMPOSITAE
0CC”RRENCE
MASAHIKO AND
CONTPCT
MUTO,
CHIDORI
Dermatology,
TOSHIHIKO
YAMAMOTO,
RITSUKO HAYAKAWA, YASUKOOCINO, KAYOKO ARIS AND YAENO ARIMA Division of Dermatology. Nagoya University Branch Hospital
ASAGRMI
Yamaguchi
Univ.
of
School
In the past 10 years we carried out the patch testings of formaldehyde (F) and imidazolidinyl urea (G: Germal@ 115. a formaldehyde releasing agent) on 358 patients with skin diseases. 23 (6.4 t) oul of 358 were posilive to F and 4 (1. 1 %)werepositive and 3 (0.8 X1 were doubtful positive to G. 2 reacted to both of F and G. one was it to C and i? to F and one was positive to G but negative to F. Out of 3 who were i? to G. 2 were positive and one was negative to F. 2 out of 23 positively reacted to F were also postlive and 2 were doubtful positive to G. C is the most commonly used cosmelic preservative in Europe and U.S.. but UP to date it has not been permitted to use in Japan. If Germal@ 115 is used in cosmetics as a preservative in our country, 8.7% (2/23) to 17.4%(4/23) of the sensitized with formaldehyde may develope allergic contact dermatitis due to Germala 115.
We have observed familial occurrence of contact allergy to The proband wor!ang at flower shop for compositae plants. She had devel20 years was a 47-year-old Japanese female. oped symptoms of contact allergy to the plant at 42 years of She was positive by patch testing for both the composage. itae plants and a liposoltile component extracted from the plant by TLC analysis. Her younqer unaffected brother, a45 year-old Japanese male, showed a suularly positive reaction These findings suggest that by patch testxng with the plant. genetic factors might be involved in the development of contact allergy to the compositae plants.
SUPPRESSIVEEFFECTOF ORALLY ADMINISTERED CYCLOSPORIN A 'Ill FLARE-UPREACTIONON MURINECONTACT HYPERSENSITIVITY
DELAYEDPRURITIC PAPULAR REACTION AT THE SITES OF PRICK TESTS WITH ENVIRONMENTALALLERGENS--ITSPOSSIBLE PATHOGENETIC RELATIONSHIPTO PRURIGO NODULES MISAKO TANAKA AND HACHIRO TAGAMI Department of Dermatology, Tohoku Univ.
School
of
MASARU NATSUAKI, NORIKOYANASHITA AND SEICHIROSAGANI of Dermatology. Hyogo College of MEdicme
Medicine,
Department
Sendai Observation in 19 out of 4’3 subjects at the sites of prick teats with several environmental antigens such as mosquitoes, Candida, house dust mites, and pollen antigens disclosed pruritic papules after 72 hours formation of solid, discrete, or later. These reactions were observed in patients who had prurigo nodularis or atopic dermatitis associated with pruriginous nodules regardless of the presence of immediate skin reactivity to those antigens. Histologically, the lesions of prurigo nodularis and those papules producted at prick test sites showed similar patterns of inflammation and infiltrating cells. These results suggest that intradermal inoculation of environmental allergens plays an important role in the pathogenesis of pruriginous pawle~ in patients particularly in those with atopic with gruriginoua lesions, dermatitis.
Cxlosoorm A (‘&A) is well known as one of the most efficient immunosuppressive drugs. In this study, we tested whelher or not orally admmlstcred CyA showed the suppressive effect to flare-up reaction on murine contact hypersensitivity reaction (CHR) by using Rechallenge system, our original experimental system as a model of chronic contact dermatitis. As the result, it was found that
significant
suppression
when
CyA
administered
was
of
ear
to
swelling
animals
at
was the
recognized fame
only
time
as and the suppressive erfect showed the dowdependent Imanner. Therefore, It is suggested that CyA treatment may be useful for the therapy of chronic contact dermatitis. rechallenge
547
548
AN IN VITRO STUDYON THE CROSS-REACTIONBETWEEN PIROXICAM PHGTOSENSITIVITYAND TBIOSALICYLATEHYPERSENSITIVITY
STATISTICAL AND HISTCCHEMICAL STATIC CANCER OF THE StilN
TAKESHI BARIYA*, KAZUKO KITAMURA, JUNK0 OSAWA, ZENRO IKEZAWA Yokohama City AND HIROSHI NAKAJINA Dept. of Dermatology. Univ. School of Medicine. Shiseido Research Center*.Yokohana
A FUJIOKA, Department School of
nonsteroidal well known as a Piroxicarn(PXM)is photocontact which causes antiminflamatory drug. dermatitis. Recently we revealed that there is a cross-reactionbetween photosensitivityto PXM and contact sensitivity to thimerosal(TMS)and thiosalicylate(TOS)by in viva skin test in guinea pigs. Then, we investigated lymphocyte proliferation by a this crossaa3ction test (LPT) using lymph node cells(LNCs) taken from guinea pigs sensitized with TOS. LNCs from the guinea pig sensitized with TOS showed a siginificantlyproliferative response to peritoneal exudate cells(PEC) previously photohapten-modified by treatmentwith PXM plus ultraviolet A. while LNCs cultured with PEC treated with only PXM did crossax?activity between not. From this result, the photosensitivityto PXM and contact sensitivity to TOS was confirmedby LF'T.
44 cases of metastatlc cancer of the skin for 20 years I” our department were investigated. All of and primary them were diagnosed histologically, lesions were confIrmed. According to the classlflcatlon based on primary lesion, I7 cases of the 10 cases of the breast cancer, 7 lung cancer, cases of stomach cancer, 3 cases of uterus cancer and 7 case8 of the other cancers were recognized. stomach and The number of the lung, breast, the other authors cancers was large such as report. Stomach cancer mainly examined histochemparadoxical ConA and PAS lcal ly. Alclan blue, staining were performed. Expression of EGF, EGFR, also were Investigated. The character of the studled by metastatlc cancer of the skin was these analysis.
238
T ASAI, of Medicine.
ANALYSIS
TTAKEvIUF?AANDK~WVEYAW. Kitasato Sagamihara
Dermatology,
OF
META-
Un~verslty