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Substance P-immunoreactive fibers in the trigeminal sensory nuclei of snakes with infrared sensitivity
$99 S U B S T A N C E P - I M M U N O R E A C T I V E F I B E R S IN THE T R I G E M I N A L NUCLEI OF SNAKES WITH INFRARED SENSITIVITY
SENSORY
T E T S U O K A D O T A % I , R E I J I K I S H I D A 1 , R I C H A R D C__~.G O R I S ~1, T O Y O K A Z U ~KI-3-, and SHIN-ICHI TERASHIMA2 1)Dept. Anat., Y o k o h a m a C i t y U. Sch. Med., 2-33 U r a f u n e - c h o , M i n a m i - k u , Y o k o h a m a , 232 Japan; 2) Dept. Physiol. U. R y u k y u s Sch. Med., 207 Ueharamiyazato, Nishihara-cho, Okinawa, 903-01 Japan. W i t h the p e r o x i d a s e - a n t i p e r o x i d a s e immunohistochemical method we ascertained the presence of substance Pi m m u n o r e a c t i v e f i b e r s (SP fibers) in the f o l l o w i n g n u c l e i of the t r i g e m i n a l s e n s o r y s y s t e m of the pit v i p e r A g k i s t r o d o n b l o m h o f f i : a f e w each in the p r i n c i p a l n u c l e u s and the m a i n neuropil of the lateral descending nucleus; a moderate number in the n u c l e u s of the m e s e n c e p h a l i c t r a c t and the d e s c e n d i n g n u c l e u s (with the e x c e p t i o n of the c a u d a l s u b n u c l e u s and the m e d i o d o r s a l part of the i n t e r p o l a r s u b n u c l e u s ) ; and a large n u m b e r in the c a u d a l s u b n u c l e u s , the m e d i o d o r s a l part of the interpolar subnucleus, and the marginal neuropil of the lateral descending nucleus. Since this marginal neuropil contains only C f i b e r s of v a g a l origin, j u d g i n g f r o m the h e a v y r e a c t i v i t y o b s e r v e d at this l o c a t i o n , it is h i g h l y p r o b a b l e that all the vagal C fibers of the marginal neuropil are SP fibers.
SUBSTANCE P IMMUNOREACTIVETHALAMOCAUDATE NEURONS IN THE CAT TETSUO SUGIMOTO, MASAHIKO TAKADA*, TAKESHI KANEKO* and NOBORU MIZUNO, Department of Anatomy ( i s t D i v i s i o n ) , Faculty of Medicine, Kyoto University, Kyoto 606, Japan. Substance P in the caudate nucleus has generally been considered i n t r i n s i c in i t s nature. We found substance P immunorectivity in some thalamocaudate neurons in the centre m6dian-parafascicular complex (CM-Pf) in the cat. Under Nembutal anesthesia (35 mg/kg, i . p . ) , microinjection of horseradish peroxidase (HRP) was done into the caudate nucleus, and 2 days l a t e r colchicine was injected into the l a t e r a l v e n t r i c l e . The cats were perfused 36 hours a f t e r the colchicine i n j e c t i o n . For demonstration of neurons containing retrogradely transported HRP, a series of frozen frontal sections were treated with tetramethyl benzidine. In another series of sections adjacent to those containing HRP-labeled neurons, substance P immunoreactivity was examined. The sections were treated for exhaustion of HRP r e a c t i v i t y , and then incubated with rabbit anti-substance P antiserum, and b i o t i n y l a t e d goat antibody against rabbit IgG. Subsequently, they were f u r t h e r incubated with a v i d i n - b i o t i n y l a t e d HRP complex. In these sections neurons with substance P immunoreactivity were d i s t r i b u t e d in the Pf, ventromedial part of the CM, posterior paraventricular nucleus, habenular nucleus, subparafascicular nucleus, circumventricular gray, posterior hypothalamus, ventral tegmental area, i n t e r f a s c i c u l a r nucleus and f i e l d of Forel. The CM-Pf neurons with substance P immunoreactivity had globular or polygonal cell bodies (average diameter: 12 X 18 um) with 3-5 primary dendrites. The sections containing substance P-positive CM-Pf neurons were compared with the adjacent sections treated for detection of retrogradely transported HRP; 5-20% of the substance P-positive CM-Pf neurons were labeled with HRP injected into the caudate nucleus.
SENSORY
T E T S U O K A D O T A % I , R E I J I K I S H I D A 1 , R I C H A R D C__~.G O R I S ~1, T O Y O K A Z U ~KI-3-, and SHIN-ICHI TERASHIMA2 1)Dept. Anat., Y o k o h a m a C i t y U. Sch. Med., 2-33 U r a f u n e - c h o , M i n a m i - k u , Y o k o h a m a , 232 Japan; 2) Dept. Physiol. U. R y u k y u s Sch. Med., 207 Ueharamiyazato, Nishihara-cho, Okinawa, 903-01 Japan. W i t h the p e r o x i d a s e - a n t i p e r o x i d a s e immunohistochemical method we ascertained the presence of substance Pi m m u n o r e a c t i v e f i b e r s (SP fibers) in the f o l l o w i n g n u c l e i of the t r i g e m i n a l s e n s o r y s y s t e m of the pit v i p e r A g k i s t r o d o n b l o m h o f f i : a f e w each in the p r i n c i p a l n u c l e u s and the m a i n neuropil of the lateral descending nucleus; a moderate number in the n u c l e u s of the m e s e n c e p h a l i c t r a c t and the d e s c e n d i n g n u c l e u s (with the e x c e p t i o n of the c a u d a l s u b n u c l e u s and the m e d i o d o r s a l part of the i n t e r p o l a r s u b n u c l e u s ) ; and a large n u m b e r in the c a u d a l s u b n u c l e u s , the m e d i o d o r s a l part of the interpolar subnucleus, and the marginal neuropil of the lateral descending nucleus. Since this marginal neuropil contains only C f i b e r s of v a g a l origin, j u d g i n g f r o m the h e a v y r e a c t i v i t y o b s e r v e d at this l o c a t i o n , it is h i g h l y p r o b a b l e that all the vagal C fibers of the marginal neuropil are SP fibers.
SUBSTANCE P IMMUNOREACTIVETHALAMOCAUDATE NEURONS IN THE CAT TETSUO SUGIMOTO, MASAHIKO TAKADA*, TAKESHI KANEKO* and NOBORU MIZUNO, Department of Anatomy ( i s t D i v i s i o n ) , Faculty of Medicine, Kyoto University, Kyoto 606, Japan. Substance P in the caudate nucleus has generally been considered i n t r i n s i c in i t s nature. We found substance P immunorectivity in some thalamocaudate neurons in the centre m6dian-parafascicular complex (CM-Pf) in the cat. Under Nembutal anesthesia (35 mg/kg, i . p . ) , microinjection of horseradish peroxidase (HRP) was done into the caudate nucleus, and 2 days l a t e r colchicine was injected into the l a t e r a l v e n t r i c l e . The cats were perfused 36 hours a f t e r the colchicine i n j e c t i o n . For demonstration of neurons containing retrogradely transported HRP, a series of frozen frontal sections were treated with tetramethyl benzidine. In another series of sections adjacent to those containing HRP-labeled neurons, substance P immunoreactivity was examined. The sections were treated for exhaustion of HRP r e a c t i v i t y , and then incubated with rabbit anti-substance P antiserum, and b i o t i n y l a t e d goat antibody against rabbit IgG. Subsequently, they were f u r t h e r incubated with a v i d i n - b i o t i n y l a t e d HRP complex. In these sections neurons with substance P immunoreactivity were d i s t r i b u t e d in the Pf, ventromedial part of the CM, posterior paraventricular nucleus, habenular nucleus, subparafascicular nucleus, circumventricular gray, posterior hypothalamus, ventral tegmental area, i n t e r f a s c i c u l a r nucleus and f i e l d of Forel. The CM-Pf neurons with substance P immunoreactivity had globular or polygonal cell bodies (average diameter: 12 X 18 um) with 3-5 primary dendrites. The sections containing substance P-positive CM-Pf neurons were compared with the adjacent sections treated for detection of retrogradely transported HRP; 5-20% of the substance P-positive CM-Pf neurons were labeled with HRP injected into the caudate nucleus.