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SUN-PP268: Lack of Coordination between Phosphorus Dietary Ingestion and Phosphate Binders Prescription in Hemodialysis Patients
Vitamins, antioxidants and minerals
Vitamins, antioxidants and minerals SUN-PP268 LACK OF COORDINATION BETWEEN PHOSPHORUS DIETARY INGESTION AND PHOSPHATE BINDERS PRESCRIPTION IN HEMODIALYSIS PATIENTS A. Sanz-Paris1 , A. Caverni2 , N. Gimenez1 , L.-M. Lou3 , D. Boj1 , B. Lardies1 , L. Perez-Fernandez1 , R. Caramelo3 , I. Azcona1 , on behalf of Nefrologia y Nutricion EBRO. 1 Nutrition Unit, Miguel Servet Hospital, 2 Nutrition Unit, Alcer Ebro, 3 Nephrology, Miguel Servet Hospital, Zaragoza, Spain Rationale: Elevated plasmatic phosphorus level in hemodialysis patients is associated with cardiovascular morbidity and bone involvement. It can be controlled through diet and taking phosphate binders. The aim of this study is to assess the intake of dietetic phosphorus of our hemodialysis patients and its relation with phosphate binders prescription. Methods: The study was performed in 48 patients on chronic hemodialysis, after informed consent and acceptance by the local Ethics Committee. Analytical data and three 24 h dietary surveys (day of dialysis, dialysis-day and Sunday), which are analyzed according BEDCA tables are collected. We compare means between taking phosphate binders (37) and those who do not take (11) with the non-parametric test of Mann Whitney. Results: 1. No differences were found between the three days about the composition of the diet. 2. The plasma phosphate levels are higher among those taking binders (p: 0.02) despite the intake of protein, calories and ratio P/protein was equal in both groups and phosphorus intake is lower (0.01). 3. Patients usually take phosphate binders at lunch and dinner, but not in the other meal intakes. The average intake of phosphorus ingested without associated to binders was 379+199 mg. Conclusion: It would be important to educate patients about the phosphorus content of foods to adapt the dose of phosphate binders to the rations of phosphorus. Disclosure of Interest: None declared
SUN-PP269 THE MAJOR DETERMINANTS OF ANTIOXIDANT CAPACITIES IN CHOCOLATE AND COCOA PRODUCTS A. Kondrashov1 , R. ˇ Sevˇ cík2 . 1 First Faculty of Medicine, Charles University in Prague, 2 Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic Rationale: The goal of this paper is to elucidate the relationship between bioactive compounds content and the antioxidant capacity of chocolates and cocoa products. Foodstuffs such as chocolate were chosen as major sources of dietary antioxidants in Western Populations. Methods: This study examines dark and milk chocolates, cocoa beans and cocoa powders. The choice of chocolates consisted of twenty-three of dark chocolate samples and four of milk chocolates. The set of cocoa products include five samples of cocoa beans and two samples of cocoa powders. Measurements of antioxidant capacities were performed using
S123 the DPPH free radical assay. The total phenolic content of chocolates and cocoa products was evaluated simultaneously according to the Folin-Ciocalteau colorimetric assay. The chocolate alkaloids such as theobromine and caffeine were evaluated in all samples by selective HPLC. Results: The significant positive correlation between the antioxidant capacity and the total phenolic content was found in all samples analyzed (r = 0.95, p < 0.001 for DPPH assay). Moreover, chocolate alkaloids also showed a positive correlation with the total antioxidant capacity in all samples (r = 0.80, p < 0.001 for theobromine and r = 0.73, p < 0.01 for caffeine respectively). In chocolate and cocoa products total phenolic content broadly varies. The lowest polyphenolic content from 721 to 1215 mg/kg was found in milk chocolates compared to 3537 10249 mg/kg in dark chocolates. The highest polyphenolic content between 12504 and 18380 mg/kg was detected in cocoa beans and cocoa powders respectively. Conclusion: The results of the study have shown positive correlation of the antioxidant capacity with total phenolic and alkaloid content. The study results may explain the positive health related properties of dark chocolates described in the current literature. Disclosure of Interest: None declared
SUN-PP270 RARE EARTH ELEMENT (REE) CONTAMINATION OF PN SOLUTIONS AND THEIR ACCUMULATION IN THE BONES OF PN PATIENTS A.L. Galusha1,2 , P.J. Parsons1,2 , P.C. Kruger2 , L.J. Howard3 . 1 Department of Environmental Health Sciences, University at Albany, 2 Laboratory of Inorganic and Nuclear Chemistry, Wadsworth Center, New York State Department of Health, 3 Department of Medicine, Division of Gastroenterology, Albany Medical College, Albany, United States Rationale: Patients on long-term PN can be exposed to trace contaminants, e.g., aluminium, in PN solutions. We previously reported excessive accumulation of Al in the bones of PN patients. Here we report additional analyses of the bones, obtained post-mortem, for REEs. Methods: Trabecular bones were collected at autopsy from 7 PN patients who received therapy for 2 21 (ave. 15) years; control bones consisted of 18 samples from hip/knee replacement surgeries. Digested bones and PN solutions were analyzed for REEs by inductively coupled plasma tandem mass spectrometry. Non-parametric statistics were used to explore differences between patient and control bones. Bone REE content was normalized to known values for the earth’s upper crust. Results: All REEs were detected in patient bone samples. REE content varied from 3 to 16,000 ng/g. In the control group Lanthanum, Cerium, and Neodymium were detected in 16 of the samples and Praseodymium in 15; the remaining REEs were detected in <5 of the samples. PN bones were significantly enriched (p < 0.001 0.0004) with REEs compared to controls. Gadolinium was highly enriched in two of the patient bones. Analysis of PN solutions revealed trace contamination with many REEs (10 3000 ng/L), and the REE pattern was similar to that found in PN bones, normalized to crustal content. Conclusion: These data indicate that patients on long-term PN are exposed to REEs at trace levels. The likely source of
Vitamins, antioxidants and minerals SUN-PP268 LACK OF COORDINATION BETWEEN PHOSPHORUS DIETARY INGESTION AND PHOSPHATE BINDERS PRESCRIPTION IN HEMODIALYSIS PATIENTS A. Sanz-Paris1 , A. Caverni2 , N. Gimenez1 , L.-M. Lou3 , D. Boj1 , B. Lardies1 , L. Perez-Fernandez1 , R. Caramelo3 , I. Azcona1 , on behalf of Nefrologia y Nutricion EBRO. 1 Nutrition Unit, Miguel Servet Hospital, 2 Nutrition Unit, Alcer Ebro, 3 Nephrology, Miguel Servet Hospital, Zaragoza, Spain Rationale: Elevated plasmatic phosphorus level in hemodialysis patients is associated with cardiovascular morbidity and bone involvement. It can be controlled through diet and taking phosphate binders. The aim of this study is to assess the intake of dietetic phosphorus of our hemodialysis patients and its relation with phosphate binders prescription. Methods: The study was performed in 48 patients on chronic hemodialysis, after informed consent and acceptance by the local Ethics Committee. Analytical data and three 24 h dietary surveys (day of dialysis, dialysis-day and Sunday), which are analyzed according BEDCA tables are collected. We compare means between taking phosphate binders (37) and those who do not take (11) with the non-parametric test of Mann Whitney. Results: 1. No differences were found between the three days about the composition of the diet. 2. The plasma phosphate levels are higher among those taking binders (p: 0.02) despite the intake of protein, calories and ratio P/protein was equal in both groups and phosphorus intake is lower (0.01). 3. Patients usually take phosphate binders at lunch and dinner, but not in the other meal intakes. The average intake of phosphorus ingested without associated to binders was 379+199 mg. Conclusion: It would be important to educate patients about the phosphorus content of foods to adapt the dose of phosphate binders to the rations of phosphorus. Disclosure of Interest: None declared
SUN-PP269 THE MAJOR DETERMINANTS OF ANTIOXIDANT CAPACITIES IN CHOCOLATE AND COCOA PRODUCTS A. Kondrashov1 , R. ˇ Sevˇ cík2 . 1 First Faculty of Medicine, Charles University in Prague, 2 Faculty of Food and Biochemical Technology, Institute of Chemical Technology in Prague, Prague, Czech Republic Rationale: The goal of this paper is to elucidate the relationship between bioactive compounds content and the antioxidant capacity of chocolates and cocoa products. Foodstuffs such as chocolate were chosen as major sources of dietary antioxidants in Western Populations. Methods: This study examines dark and milk chocolates, cocoa beans and cocoa powders. The choice of chocolates consisted of twenty-three of dark chocolate samples and four of milk chocolates. The set of cocoa products include five samples of cocoa beans and two samples of cocoa powders. Measurements of antioxidant capacities were performed using
S123 the DPPH free radical assay. The total phenolic content of chocolates and cocoa products was evaluated simultaneously according to the Folin-Ciocalteau colorimetric assay. The chocolate alkaloids such as theobromine and caffeine were evaluated in all samples by selective HPLC. Results: The significant positive correlation between the antioxidant capacity and the total phenolic content was found in all samples analyzed (r = 0.95, p < 0.001 for DPPH assay). Moreover, chocolate alkaloids also showed a positive correlation with the total antioxidant capacity in all samples (r = 0.80, p < 0.001 for theobromine and r = 0.73, p < 0.01 for caffeine respectively). In chocolate and cocoa products total phenolic content broadly varies. The lowest polyphenolic content from 721 to 1215 mg/kg was found in milk chocolates compared to 3537 10249 mg/kg in dark chocolates. The highest polyphenolic content between 12504 and 18380 mg/kg was detected in cocoa beans and cocoa powders respectively. Conclusion: The results of the study have shown positive correlation of the antioxidant capacity with total phenolic and alkaloid content. The study results may explain the positive health related properties of dark chocolates described in the current literature. Disclosure of Interest: None declared
SUN-PP270 RARE EARTH ELEMENT (REE) CONTAMINATION OF PN SOLUTIONS AND THEIR ACCUMULATION IN THE BONES OF PN PATIENTS A.L. Galusha1,2 , P.J. Parsons1,2 , P.C. Kruger2 , L.J. Howard3 . 1 Department of Environmental Health Sciences, University at Albany, 2 Laboratory of Inorganic and Nuclear Chemistry, Wadsworth Center, New York State Department of Health, 3 Department of Medicine, Division of Gastroenterology, Albany Medical College, Albany, United States Rationale: Patients on long-term PN can be exposed to trace contaminants, e.g., aluminium, in PN solutions. We previously reported excessive accumulation of Al in the bones of PN patients. Here we report additional analyses of the bones, obtained post-mortem, for REEs. Methods: Trabecular bones were collected at autopsy from 7 PN patients who received therapy for 2 21 (ave. 15) years; control bones consisted of 18 samples from hip/knee replacement surgeries. Digested bones and PN solutions were analyzed for REEs by inductively coupled plasma tandem mass spectrometry. Non-parametric statistics were used to explore differences between patient and control bones. Bone REE content was normalized to known values for the earth’s upper crust. Results: All REEs were detected in patient bone samples. REE content varied from 3 to 16,000 ng/g. In the control group Lanthanum, Cerium, and Neodymium were detected in 16 of the samples and Praseodymium in 15; the remaining REEs were detected in <5 of the samples. PN bones were significantly enriched (p < 0.001 0.0004) with REEs compared to controls. Gadolinium was highly enriched in two of the patient bones. Analysis of PN solutions revealed trace contamination with many REEs (10 3000 ng/L), and the REE pattern was similar to that found in PN bones, normalized to crustal content. Conclusion: These data indicate that patients on long-term PN are exposed to REEs at trace levels. The likely source of