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Table of contents — volume 24
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TABLE OF CONTENTS - VOLUME 24 Steroid Radioimmunoassay: Contribution of Standards to Blank Values. MARIJKE FROLICH, W. TERMORSHUIZEN, ELLY KENTER and A. J. MOOLENAAR . . . . . . . . . , . , .
1
The Effects of Synthetic Estrogens on the Metabolic Clearance and Production Rates of Estrone and Estradiol. C. LONGCOPE, D. WATSON and K.I.H. WILLIAMS. . . . . . . .
15
Determination of Progesterone and of Free and Conjugated Estrogens in Pregnant and Pseudo-Pregnant Rats. S. DE LAUZON, F. UHRICH, S. VANDEL, N. CITTANOVA and M.F.JAYLE.................... . . t
31
The Pituitary-Gonadal Axis Before Puberty: Effects of Various Estrogenic Steroids in the Ovariectomized Rat. J. C. MCPHERSON III, J. C. ELDRIDGE, A. COSTOFF and V.B.MAHESH.......................
41
Estradiol-178-Hydroxysteroid Dehydrogenase Activity in Preimplantation Rat Embryos. SUDHANSU K. DEY and Z.DICKMANN.. . . . . . . . . . . . . . . . . . . ..a
57
Synthesis and Antiestrogenic Activity of 2,4-BisfBromoethyl)-Estrone Methyl Ether: A Bifunctional Alkylating Steroid. NA~SIMHA RAO. ~NAMARLAPUDI, FREDERICK SWEET and JAMES C. WARREN . . _ . , , . . . . . . , . . . . . ,
63
Intramolecular Cyclisation Reactions of fig-Cholan24-Amide, 3a-Acetoxy-Ss-Cholan-24-Amide, and 3a-Acetoxy-Se-Cholan-24-01, P. HODGE, G. M. PERRY and M. POLLARD. . A . . . . . . . . . . , . . . . . . . .
79
Radioimmunoassay
W. A. COLBURN . . . . .
95
Studies on the Site of Origin of the Androgen Binding Protein Present in Epididymal Cytosol from Mature Intact Rabbits. BENJ~IN J. DANZO, BARBARA C. ELLER and MARIE-CLAIRE ORGEBIN-CRIST. . . . . . . . . , . . . .
107
Androgen Binding Protein (ABP) in Rabbit Testis and Epididymis. S. C. WEDDINGTON, W. S. MCLEAN, S. N. NAYFEH, F. S. FRENCH, V. HANSSON and E. M. RITZEN .
123
A Simple Preparation of Z-Methyl-5a-Cholest-Z-enc. M. ADINOLFI, M, PARRILLI, G. BARONE and G, LAONIGRO , . .
135
Errata...
137
for Prednisone.
. . . . . . . . . . . . . . . . . . . . . . .
900
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Testosterone Formation in Testis of the Innnature Androgen Insensitive Pseudohermaphrodite Male Rat (Stanley-Gumbreck Rat). P. A. ARONIN, J. C. COFFEY, F. S. FRENCH, and S. N. NAYFEH. . . . . . . . . . . . . .
139
Steroid Biosynthesis by Reptilian Adrenals. A. Z. MEHDI and T. SANDOR . . . . . . . . . . . . . . . .
151
Nutritional Effect of Possible Intermediates of Phytosterol Dealkylation in the Silkworm, ~ombyz mori. MASUO MORISAKI, HIROSHI OHOTAKA, NOR10 AWATA, NOBUO IKEKAWA, YASUHIRO HORIE, and SHOICHI NAKASONE . . .
165
Isolation of ACTH-Induced Protein from Adrenal Perfusate. S. G. LAYCHOCK and R. P. RUBIN. . . . . . . .
177
New Estetrols in Pregnancy Urine. N. F. TAYLOR and C.H.L. SHACKLETON . . . . . . . . . . . . . . . . . . . .
185
Plasma Testosterone During Nocturnal Sleep in Normal Men. RAUL C. SCHIAVI, DAVID M. DAVIS, DANIEL WHITE, ADELE EDWARDS, GEORGE IGEL, and CHARLES FISHER. . . . . .
191
Brisbagenin and Brisbenone, Two New Spirostanes from Species. K. JEWERS, M. B. BURBAGE, G. BLUNDEN, and W. J. GRIFFIN . . . . . . . . . . . . . .
203
The Effect of Antiestrogens on the Nuclear Binding of the Estrogen Receptor. THOMAS S. RUH and MARYF.RUH.......................
209
Estriol in Pregnancy. III. Development, Comparison and Use of Specific Antisera for Rapid Radioimmunoassay of Unconjugated Estriol in Pregnancy Plasma. HIDEHIKO KATAGIRI, FRANK Z. STANCZYK, and UWE GOEBELSMANN . . . . . . . . . . . . . . . . . . . . .
225
Bile Acids XLII. Preparation of 5a-Cholestan-26-oic Acids from Kryptogenin. ME1 ME1 MUI, S. Y. KAMAT, and WILLIAM H. ELLIOTT. . . . . . . . . . . . . . . . . .
239
Plasma Cortisol Concentrations in Two Teleost Fishes, auratus L.: A anguilla L. and Carassius Comparison of Two Assay Systems. GORDON HARGREAVES and JACQUELINE PORTHE-NIBELLE . . . . . . . . . . . . . .
251
Acetylation and Hydroxylation of 5a-Androstane-3s,17sdiol by Prostate and Epididymis. PETER OFNER and RICHARD L. VENA, and ROBERT F. MORFIN . . . . . . . . . .
261
CordyZine
Anguilla
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901
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Effects of Prior Androgen Exposure upon Adrenal Function in Maturing Rats. JUDITH A.RAMALEY. . . . . . . . . . . . Plasma Androgens in Idiopathic Hirsutism. and V.H.T. JAMES . . . . . . . . . . . .
281
C.M. ANDRE
. . . . . . . . . 295
Conversion of Testosterone-4-14C to Androgens of the 5a-Androstane Series by a Normal Human Ovary. O.W. SMITH, P. OFNER and R.L. VENA . . . . . . . . . . . .
311
A Non-Chromatographic Radioimnunoassay for Serum Dehydroepiandrosterone Using a Mixture of Antisera. HISAHIKO SEKIHARA and NAKAAKI OHSAWA . . . . . . . . . . .
317
A Sephadex Column Radioimmunoassay of Unconjugated Estriol in Pregnancy Plasma. JAMES E. CHRISTNER and MARION C. FETTER . . . . . . . . . . . . . . . . . . . . .
327
Facile Synthesis of Deuterated Estrogens.
. .
343
Conversion of 20a-Hydroxy-4-Pregnen-3-One to 20a-Hydroxy-5a-Pregnan-3-One and 5a-Pregnane-3a,20a-Diol by Rat Anterior Pituitary. FELICIA V. NOWAK and HARRY J. KARAVOLAS . . . . . . . . . . . . . . . . . . . .
351
3-Epi-20-Hydroxyecdysone from Meconium of the Tobacco Hornworm. MALCOLM J. THOMPSON, JOHNN. KAPLANIS, WILLIAME. ROBBINS,SAMSON R. DUTKY and HERBERT N. NIGG..
359
In vitro
R.C. MURPHY
Synthesis of 24,28-Iminofucosterol and Its Inhibitory Effects on Growth and Steroid Metabolism in the Silkworm,
Bombyx mori.
YOSHINORI FUJIMOTO, MASUOMORISAKI,
NOBUO IKEKAWA, YASUHIRO HORIE and SHOICHI NAKASONE . . . .
367
A Novel Bufadienolide Synthesis. A. BELANGER, P. BRASSARD, G. DIONNE and CH. R. ENGEL. . . . . . . . . .
377
245-Methylcholestane-38,5a,66,25_Tetrol 25-Monoacetate A Novel Polyhydroxylated Steroid from an Alcyonarian. J. MICHAEL MOLDOWAN, BERNARD M. TURSCH and CARL DJERASSI .
387
Marine Sterols. V. Isolation and Structure of Occelasterol, a New 27-Norergostane-type Sterol, from MASARU KOBAYASHI an Anne1 ida, Peeudopotamilla occeZata. and HIROSHI MITSUHASHI . . . . . . . . . . . . . . . . .
. 399
Identification by Gas Chromatography-Mass Spectrometry of Intermediates in the Aromatization of Modified Clg Steroids by Human Placental Microsomes. W. EMMETT BRASELTON, JR., JAMES C. ORR and LEWIS L. ENGEL.
411
Errata..........................
435
902
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Two High Affinity Estrogen Binding Proteins of Different Specificity in the Immature Rat Uterus Cytosol. GEORGES MICHEL, INGRID JUNG, ETIENNE-EMILE BAULIEU, CLAUDE AUSSEL and JOSE URIEL . . . . . . . . . . . . . . .
437
Regulation of Aldosterone Synthesis. A. PEYTREMANN, R. D. BROWN, W. E. NICHOLSON, D. P. ISLAND, G. W. LIDDLE and J. G. HARDMAN. . . . . . . . . . . . . . . . . . . . .
451
The Synthesis and Biological Activity of 25,26Dihydroxy-Cholecalciferol, a Polar Metabolite of Vitamin D3. J. REDEL, P. A. BELL, N. BAZELY, Y. CALANDO, F. DELBARRE and E. KODICEK . . . . . . . . . .
463
Antiserum to 5a-Ditiydrotestosterone: Production, Characterization and Use in Radioimmunoassay. S. BAUMINGER, F. KOHEN, H. R. LINDNER and A. WEINSTEIN
477
. .
The Influence of Enzymes on the Estrogen Receptors of Human Uterus and Breast Carcinoma. ROLAND HAHNEL, ELLA TWADDLE and LARAINE BRINDLE . . . . . . . . . . . . .
489
Tissue Androgens and Androphilic Proteins in Rat Epididymis During Sexual Development. R. S. CALANDRA, E. J. PODESTA, M. A. RIVAROLA and J. A. BLAQUIER . . . . .
507
In vitro Metabolism of Estrone, 2,4,6,7-3H and 4-Androstene-3,17-Dione-1,2-3H in Submandibular Gland and Submandibular Gland Cancer Tumor. TAWFIK M. A. ElATTAR . . . . . . . . . . . . . . , . . . .
519
Interaction of Estradiol and ACTH in the Regulation of Adrenal Corticosterone Production in the Rat. HOWARD D. COLBY and JULIAN I. KITAY. . . . . . . . . .
527
The Uptake and Metabolism of Labelled Testosterone by the Brain and Pituitary of the Male Rhesus Monkey (Macaca rmdatta). LEON J. SHOLITON, BRANDON B. TAYLOR and H. PAUL LEWIS. . . . . . . . . . . . . . . . . . .
I
.
. .
537
Desmosteryl Sulfate and Desmosterol in Hamster Epididymal Spermatozoa. G. BLEAU and W. J. A. VANDEN HEUVEL . . . . . . . . . . . . . . . . . .
549
Biliary Metabolites of Testosterone and Testosterone Glucosiduronate in the Rat. MICHIO MATSUI, YUKO KINUYAMA and MISAKO HAKOZAKI. . . . . . . . . . . . .
557
Regional Differences in Androgen Thresholds of the Epididymis of the Castrated Rat. GAYATRI GUPTA, M. RAJALAKSHMI and M. R. N. PRASAD . . . . . . . . . . . .
575
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903
A Direct Determination of Plasma Aldosterone. MARIE T. PHAM-HUU-TRUNG and PIERRE CORVOL . . . . . . . . . .
587
Receptor Progesterone Complex in the Nuclear Fraction of the Rat Uterus: Demonstration by 3H-Progesterone Exchange. A.J.W. HSUEH, E. J. PECK, JR., and J. H. CLARK. . . . . . . .
599
A New Class of Long-Acting Hormonal Steroid Pr'eparation: Synthesis of Dimeric Ethynodiol and Nortestosterone, of Dimeric and Trimeric Androgens and of some Dimeric Combinations of Steroids. HERBERT KUHL and HANS-DIETER TAUBERT . . . . . . . . . . . . . . . . . . . , .
613
A Formal Synthesis of 18-Hydroxyestrone. D. K. BANERJEE, S. D. VENKATARAMU, and P. K. SEN. . . . . . . . . . . . . . .
627
Trace Analysis of Ecdysones by Gas-Liquid Chromatography, Radioimmunoassay and Bioassay. DAVID W. BORST and JOHN D. O'CONNOR. . . . . . . . . . . . . . . . . . . . . . .
637
Temporal Changes in Serum Estradiol and Prolactin in Immature Female Rats Given a Single Injection of Estradiol Benzoate. H. C. CHENG and D. C. JOHNSON. . . . . .
657
A Radioimmunoassay of Plasma Estradiol-176 with the Use of Polyethylene Glycol to Separate Free and Antibody-bound HARVEY S. SCHILLER and MARGARET A. BRAMMALL . . . . Hormone.
665
Effects of Anterior Pituitary Hormones on Hepatic Corticosterone Metabolism in Rats. HOWARD D. COLBY, JOE H. GASKIN, and JULIAN I. KITAY. . . . . . . . . . . . . .
679
4-Hydroxyestrone: A New Metabolite of Estradiol-178 from Humans. J. G. WILLIAMS, C. LONGCOPE, and K.I.H. WILLIAMS . . . . . . . . . . . . . . . . . . . . . . .
687
Ontogeny of Mitochondrial Steroidogenesis in the Guinea S. A. SHOLL . . . . , . . . . . . . . . . . . . . Pig Gonad.
703
The Steroidogenic Ability of Various Cell Types of the Equine Ovary. DAMODAR K. MAHAJAN and LEO T. SAMUELS. . , . .
713
The Prolonged Progestational and Anti-estrogenic Activity of 6,118-Dichloro-3B,17-Dihydroxy-19-nor-4,6-Pregnadien20-One 17-Acetate 3B-Benzoate (GR2/2132). H. G. GILBERT, E. A. WOOLLETT, and K. J. CHILD . . . . . . . . . . . . . . .
731
3-Methoxy-1,3,5(10)-estratriene-17(R)-spiro-6'-{2'-[bis(2-chloroethyl)amino]-2'-0~0-3'oxa-3'-aza-2'-phosphorane). EMERSON L. FOSTER and ROBERT T. BLICKENSTAFF. . . . . . . , .
737
904
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Synthesis of Corticosteroid Haptens. AK10 TSUJI, MILLIE SMULOWTIZ, JULIA S. C. LIANG and DAVID K. FUKUSHIMA .
739
The Conversion of Testosterone to 7a-Hydroxy-Testosterone by Incubated Rat Testes. W. EECHAUTE, E. LACROIX and I.LEUSEN..........................
753
Estrogen Induction of 20a-Hydroxysteroid Dehydrogenase in Mouse Vaginal Tissue. KARL C. PODRATZ, THEODORE W. MUNNS and PHILIP A. KATZMAN. . . . . . . . . . . . . . . . . . . .
765
Metabolism of Progesterone in Mouse Vaginal Tissue. KARL C. PODRATZ, THEODORE W. MUNNS and PHILIP A. KATZMAN . .
775
Specific Antisera Suitable for Solid-Phase Radioimmunoassay of llg-Hydroxyandrost-4-ene-3,17-dione. P. NARASIMHA RAO, PERRY H. MOORE, JR. and J. W. GOLDZIEHER . . . , . . . . . .
793
Antisera for Radioimmunoassay of 17a-Ethynylestradiol and Mestranol. P. NARASIMHA RAO, ARMANDO DE LA PENA and JOSEPH W. GOLDZIEHER . . . . . . . . . . . . . . . . . . . .
803
Simultaneous Measurement of Unbound Testosterone and Estradiol Fractions in Undiluted Plasma at 37" C by Steady-State Gel Filtration. R. A. FISHER, D. C. ANDERSON and C. W. BURKE . . . . . . . . . . . . . . .
809
A Study on the Stability of an Estrogen-Protein Conjugate Conditions. Y. Y. TSONG and S.S.KOIDE.........................
825
Metabolism of Progesterone in Subcellular Fractions of Rat Uterus. JUDITH SAFFRAN, BONNIE K. LOESER, BERNADETTE i4. HAAS and HOMER E. STAVELY. . . . . . . . . . .
839
The Effect of Oral Contraceptive Steroid Therapy on the Urinary Metabolites of Radioactive Estrone and Estradiol-17B. ANDREA M. FEMINO, CHRISTOPHER LONGCOPE, JUNE G. WILLIAMS and KENNETH I. H. WILLIAMS. . . . . . . . .
849
Site of Conjugation of Bovine Serum Albumin to Corticosteroid Hormones and Specificity of Antibodies. TOSHIHIRO NISHINA, AK10 TSUJI and DAVID K. FUKUSHIMA . . . .
861
Studies on the Metabolism of Steroid Hormones. PART II. Reversible Steroid-Protein Interactions via Enol Intermediates. PETER TOFT . . . . . . . . . . . . . . . . .
875
Effects of Fetectomy on Plasma Estrogens and Progesterone in Monkeys (Xacaca mulatta). WILLIAM W. TULLNER and GARY D. HODGEN . . . . . . . . . . . . . . . . . . . . . . .
887
Volume 24 Table of Contents, Author
899
in vivo and Under in vitro
Index, Subject Index . .
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AUTHOR INDEX - VOLUME 24 Adinolfi, M., 135 Anderson, D.C., 809 Andre, C. M., 295 Aronin, P. A., 139 Aussel, C., 437 Awata, Norio, 165 Banerjee, D. K., 627 Barone, G., 135 Baulieu, E.-E., 437 Bauminger, S., 477 Basely, N., 463 Belanger, A., 377 Bell, P. A., 463 Blaquier, J. A., 507 Bleau, G., 549 Blickenstaff, R. T., 737 Blunden, G., 203 Borst, David, 637 Brammall, M. A., 665 Braselton, W. E., Jr., 411 Brassard, P., 377 Brindle, Laraine, 489 Brown, R. D., 451 Burbage, M. B., 203 Burke, C. W., 809 Calando, Y., 463 Calandra, R. S., 507 Cheng, H. C., 657 Child, K. J., 731 Christner, J. E., 327 Cittanova, N., 31 Clark, J. H., 599 Coffey, J. C., 139 Colburn, W. A., 95 Colby, H. D., 527, 679 Corvol, P., 587 Costoff, A., 41 Danzo, B. J., 107 David M., 191 de la Pena, Armando, 803 de Lauzon, S., 31 Delbarre, F., 463 Dey, S. K., 57 Dickmann, Z., 57 Dionne, G., 377 Djerassi, C., 387 Dutky, Samson R., 359 Davis,
Edwards, Adele, 191 Eechaute, W., 753 El Attar, Tawfik M.A., 519 Eldridge, J., 41 Eller, B. C., 107 Elliott, W. H., 239 Engel, Ch. R., 377 Engel, L. L., 411 Femino, A. M., 849 Fetter, M. C., 327 Fisher, Charles, 191 Fisher, R. A., 809 Foster, E. L., 737 French, F. S., 123, 139 Frolich, Marijke, 1 Fujimoto, Yoshinori, 367 Fukushima, David K., 739, 861 Gaskin, Joe H., 679 Gupta, Gayatri, 575 Gilbert, H. G., 731 Goebelsmann, U., 225 Goldzieher, J. W., 793, 803 Griffin, W. J., 203 Haas, B. M., 839 Hahnel, Roland, 489 Hakozaki, M., 557 Hansson, V., 123 Hardman, J. G., 451 Hargreaves, Gordon, 251 Hodge, P., 79 Hodgen, G. D., 887 Horie, Yasuhiro, 165, 367 Hsueh, A. J. W., 599 Igel, George, 191 Ikekawa, Nobuo, 165, 367 Island, D. P., 451 James, V. H. T., 295 Jayle, M. F., 31 Jewers, K., 203 Johnson, D. C., 657 Jung, I., 437
905
Kamat, s. Y., 239 Kanamarlapudi, N., 63 Kaplanis, John N, 359 Karavolas, H. J., 351 Katagiri, H., 225 Katzman, Philip A., 765, 775 Kenter, Elly, 1 Kinuyama, Y., 557 Kitay, J. I., 527, 679 Kobayashi, M., 399 Kodicek, E., 463 Kohen, F., 477 Koide, S. S., 825 Kuhl, H., 613 Lacroix, E., 753 Laonigro, G., 135 Laychock, S. G., 177 Leusen, I., 753 Lewis, H. Paul, 537 Liang, Julia S., 739 Liddle, G. W., 451 Lindner, H. R., 477 Loeser, B. K., 839 Longcope, C., 15, 687, 849 Mahajan, D. K., 713 Mahesh, Virendra, B., 41 Matsui, M., 557 Mehdi, A. Z., 151 Michel, G., 437 Mitsuhashi, H., 399 Moldowan, J. M., 387 Moolenaar, A. J., 1 Moore, Perry H., Jr., 793 Morfin, R. F., 261 Morisaki, Masuo, 165, 367 Mui, M. M., 239 Munns, Theodore W., 765, 775 Murphy, R. C., 343 McLean, W. S., 123 McPherson, J. C., III, 41 Nakasone, Shoichi, 165, 367 Nayfeh, S. N., 123, 139 Nicholson, W. E., 451 Nigg, Herbert N., 359 Nishina, Toshihiro, 861 Nowak, F. V., 351
O'Connor, John D., 637 Ofner, Peter, 261, 311 Ohotaka, Hiroshi, 165 Ohsawa, N., 317 Orgebin-Crist, M.-C., 107 Orr, J. C., 411 Parrilli, M., 135 Peck, E. J., Jr., 599 Perry, G. M., 79 Peytremann, A., 451 Pham-Huu-Trung, M. T., 587 Podesta, E. J., SO7 Podratz, Karl C., 765, 775 Pollard, M., 79 Porthe-Nibelle, Jacqueline, 251 Prasad, M. R. N., 575 Rajalakshmi, M., 575 Ramaley, Judith A., 281 Rao, P. Narasimha, 793, 803 Redel, J., 463 Ritzen, E. M., 123 Rivarola, M. A., 507 Robbins, William E., 359 Rubin, R. P., 177 Ruh, Mary F., 209 Ruh, Thomas S., 209 Saffran, J., 839 Samuels, L. T., 713 Sandor, T., 151 Schiavi, Raul C., 191 Schiller, H. S., 665 Sekihara, H., 317 Sen, P. K., 627 Shackleton, C. H. L., 185 Sholiton, Leon J., 537 Sholl, S. A., 703 Smith, Olive W., 311 Smulowitz, M., 739 Stavely, H. E., 839 Stanczyk, F. Z., 225 Sweet, Frederick, 63
S Taubert, H.-D., 613 Taylor, Brandon B., 537 Taylor, N. F., 185 Termorshuizen, W., 1 Thompson, Malcolm J., 359 Toft, P., 875 Tsong, Y. Y., 825 Tsuji, Akio, 739, 861 Tullner, W. W., 887 Tursch, B. M., 387 Twaddle, Ella, 489 Uhrich, F., 31 Uriel, J., 437
7?-EOXDI
Vandel, S., 31 VandenHeuvel, W. J. A., 549 Vena, Richard L., 261, 311 Venkataramu, S. D., 627 Warren, James C., 63 Watson, D., 15 Weddington, S. C., 123 Weinstein, A., 477 White, Daniel, 191 Williams, J. G., 687, 849 Williams, K. I. H., 15, 687, 849 Woollett, E. A., 731
907
SUBJECT INDEX - VOLUME 24 3a-Acetoxy-5B-cholan-24-amide, intramolecular cyclisation of, 79 ACTH, induced protein synthesis and release, 177 Adrenal Corticosterone production, in rat, 527 Adrenal protein release, 177 Alcyonarian, natural products from, 387 Aldosterone, biosynthesis of, 451 direct radioimmunoassay without chromatography, 587 Androgen binding proteins, in rabbits, 107 Androgenization, early, in rats, 281 Androgens, in rat tissues, 507 plasma levels in hirsutism, 295 Androphilic proteins, in rat epididymis, 507 So-Androsta-3S,17B-dial. metabolism of, 261 5a-Androstan-17B-ol-3-one, antiserum to and radioimmunoassay of in human plasma, 477 binding protein of, 107 -la-carboxy thioether, synthesis of, 477 product of testosterone-4- 14C incubated with normal human ovary, 311 regional differences of in the castrated rat, 575 Androst-4-en-68,11fi-diol-3,17-dione, 6-hemisuccinate-BSA, synthesis of, 793 Androstendione, in cancer tumor, 519 in hirsutism, 295 highly specific antisera of, 793 Androst-4-en-ll@ol-3,17-dione, Androst-4-en-17S-ol-3-one, dimeric, 613 regional differences in thresholds of the epididymis of the castrated rat, 575 Antiestrogen activity, 63 Antiestrogens, in vitro inhibition of nuclear estrogen receptor binding, 209 Antisera, for estriol radioimmunoassay, 225 Aromatization, of Clg intermediates, 411 Bioavailability, of prednisone, 95 Biosynthesis, of steroids in mare ovaries, 713 2,4-bis(bromomethyl)-3-methoxy-1,3,5[10]-trien-17-one, antiestrogenic activity of, 63 Bovine adrenal cells, regulation of aldosterone synthesis, 451 Brain, monkey, the uptake and metabolism of labelled testosterone in, 537 Bufadienolides, novel synthesis of, 377 Cardiotonic steroids, of the bufadienolide type, contribution to the synthesis of, 377 58-Cholana-3a,24-diol-3a-acetoxy, intramolecular cyclisation of, 79 5B-Cholan-24-amide, intramolecular cyclisation of, 79 5a-Cholesta-3a,7a-diol-26-carboxylic acid, preparation of, 239 Cholest-5-en-3$,26-diol-16,22-dione, preparation of, 239
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TBEOIDl
Cholest-5-en-38-01,side chain cleavage of, 703 Competitive protein binding assay (CPBA), cortisol level in teleostei, a justification by comparison with DIDA, 253 Contraceptive steroids, effect on estrogen metabolism, 849 Corticosteroid haptens, synthesis of, 739 Corticosterone metabolism, in rats, 679 Corticosterone production, in rat adrenal, 527 Cyclophosphamide, steroid derivative of, 737 Dealkylation, of phytosterol in insect, 165 Dealkylation inhibitor, in silkworm, 367 Desmosteryl sulfate, in hamster spermatozoa, 549 6, 118-Dichloro-38,17-dihydroxy-l9-nor-4,6-pregnadien-2O-one17-acetate-38-benzoate,prolonged activity of, 731 25,26-Dihydroxycholecalciferol,biological activity and synthesis of, 463 38,7a- and 3a,7a-Dihydroxy-5a-cholestan-26-oic acids, preparation of, 239 18,38-Dihydroxy-5a-25a-spirostane(brisbagenin), conversion to 3$-hydroxy-5a-25a-spirostane(tigogenin)via 5a,25a-spirost-1-en-3-one(brisbenone),203 isolation and characterisation from CordyZine cannifolia, 203 8-Ecdysone, radioimmunoassay of, 637 Ecdysones, from Meconium of the Tobacco Hornworm, 359 Epididymal androgen receptor, in rabbits, 123 Epididymis, androgen binding protein in, 107 Clg-steroid acetylation and hydroxylation of, 261 Regional differences in androgen thresholds in the castrated rat, 575 3-Epi-20-hydroxyecdysone,from Meconium of the Tobacco Hornworm, 359 24,28-Epoxystigmast-5-en-38-01,synthesis of, 367 Estetrols, in pregnancy urine, 185 Estradiol, interaction of in rat adrenal, 527 measurement of unbound fraction in plasma by steady-state gel filtration, 809 metabolism of, 849 Estradiol-dz,synthesisof, 343 Estradiol-178, metabolism of, 687 radioimmunoassay of, 665 serum levels after estradiol benzoate injection, 657 4-3H, preparation and metabolism of, 687 hydroxysteroid dehydrogenase, in morulae and blastocysts, 57 [3HlEstradiol-178-succinyl,in bovine serum albumin, 825 Estradiol binding protein, in rat, 437 Estradiol receptor, 437 Estra-1,3,5(10)-trien-3,16a,l78-triol, radioimmunoassay of, 225 radioimmunoassay of unconjugated in human pregnancy plasma, 327 Estriol-d2,synthesisof, 343
909
Estrogen, induction of 20a-hydroxysteroid dehydrogenase, 765 metabolism of, 15, 775 production of, 15 -protein, conjugate of, 825 receptor, in rat, 437 receptors, in human uterus and breast carcinoma, 489 Estrogens, effect of on serum gonadotropins, 41 in pregnancy urine, 185 in rat plasma, 31 Estrone, in cancer tumor, 519 metabolism of, 849 -d,, synthesis of, 343 17a-Ethinyl-4-estrene-36,17@diol, dimeric, 613 Ethynylestradiol, effect on estrogen metabolism, 849 17a-Ethynylestra-1,3,5(10)-trien-3,17@biol, antisera of, 803 17a-Ethynyl-3-methoxyestra-l,3,5(lO)-trien-17B-o1, antisera of, 803 Fetal gonad, steroidogenesis in, 703 Fetectomy, effects of on plasma steroids, 887 al-Fetoprotein, in rat, 437 Fucosterol-24,28-epoxide, 165 Gas chromatography-mass spectrometry, identification of Cl9 intermediates, 411 of steroids, 185 Gonadotropins (FSH and LH), effect of estrogens on, 41 Guinea pig, steroidogenesis in, 703 2B,38,14a,2OR,22R,25-Hexahydroxy-cholest-7-en-6-one (p-ecdysone), 637 Hirsutism, plasma androgen levels in, 295 unbound fractions of testosterone and estradiol in plasma, 809 Hormones, from Meconium of the Tobacco Hornworm, 359 Hydrolytic enzymes, influence on estrogen receptors and estradiol-receptor complex, 489 2-Hydroxyestradiol, as a metabolite of estradiol, 687 as metabolite of estrone and estradiol, 849 18-Hydroxyestriol, in pregnancy urine, 185 4-Hydroxyestrone, as a metabolite of estradiol, 687 18-Hydroxyestrone, synthesis of, 627 7o-Hydroxy-testosterone, formation of in testes, 753 Hydroxylation, of testosterone, 875 2Oa-Hydroxysteroid dehydrogenase, in mouse vagina, 765 24,28-Iminostigmast-5-en-3B-ol,
synthesis of, 367
Marine sterols, polyhydroxylated, 387 24-Methylcholestane derivative, from alcyonarian, 387 24S-Methylcholesta-3B,5a,6B,25-tetraol-25-acetoxy, 387
2-Methyl-5a-cholest-2-ene, preparation of from 5a-cholestan-38-01, Monkeys, plasma progesterone and estrogens after fetectomy, 887
135
27-nor-(24S)-24-Methylcholesta-5,22-dien-3B-o1, from an annelida, 399 Nuclear receptor, progesterone complex in, 599 Nutritional effect in insect of sterol derivative, 165 Oligomeres, steroid, 613 Ontogeny, of receptor, in rat, 437 Ovary, normal human, presence of A4-3-oxosteroid 5a-reductase in, 311 steroid biosynthesis in, 713 Oxaazaphosphorane, 17-Spiro, 737 Pituitary, anterior in rat, metabolism of pregn-4-en-20a-ol-3-one in, 351 monkey, the uptake and metabolism of labelled testosterone in, 537 response of to increased serum estrogen in immature female rats, 657 Pituitary hormones, effect of on corticosterone metabolism, 679 Placenta, human, Cl9 intermediates in, 411 Polyhydroxylated steroid, from alcyonarian, 387 Polyethylene glycol, preciptation of steroid antibodies with, 665 Prednisone, radioimmunoassay of, 95 5a-Pregnan-2Ca-ol-3-one, formation from 20a-DHP in anterior pituitary in rat, 351 Pregn-4-en-20a-ol-3-one, anterior pituitary metabolism of in rat, 351 Pregn-4-en-llB,17a,2l-triol-3,2O-dione, determination and comparison of level by CPBA and DIDA, 253 Progesterone, in rat plasma, 31 metabolism of by pseudohermaphrodite rat testes, 139 metabolism, in mouse vagina, 775 metabolism of in subcellular fractions of uterus, 839 receptor in rat uterus, 599 Progesterone-3H, exchange assay of, 599 Prolactin, release of in response to estradiol, 657 Prostate, C 19-steroid acetylation and hydroxylation of, 261 Puberty, adrenal function in rats, 281 a-Pyrones, synthesis of, 377 Rabbit, TeBG in, 123 Radioimmunoassay, blank values in paper chromatography, 1 for serum dehydroepiandrosterone without chromatography, 317 of Androst-4-en-llB,ol-3,17-dione, 793 of corticosteroids, 861 of estriol, 225 of plasma estradiol, 665 of prednisone, 95 of unconjugated estriol in human pregnancy plasma, 327 Rats, pregnant and pseudo-pregnant, determination of progesterone and estrogens in, 31 Rat uterus, mechanism of action of some antiestrogens in, 209 Reptilian adrenal, steroidogenesis, 151
Sephadex (G-lo), in column radioimmunoassay of unconjugated estriol in human pregnancy plasma, 327 Sleep, testosterone level in plasma during, 191 5a,25a-Spirost-1-en-3-one (brisbenone), isolation and characterisation from CordyZine sp, 203 Steroid, bifunctional alkylating, 63 Teleostei, cortisol levels in eel and goldfish plasma, 253 Testes, metabolization of testosterone in, 753 Testicular androgen binding protein (ABP), in rabbits, 123 Testosterone, formation of in pseudohermaphrodite rat testes, 139 hydroxylation of, 875 in plasma during sleep, 191 measurement of unbound fraction in plasma by steady-state gel filtration, 809 metabolization of in testes, 753 metabolization, in the brain and pituitary of the male Rhesus monkey, 537 Testosterone-17-glucosiduronate, metabolism of in the rat, 557 Uterus, estrogen receptor in, in rat, 437 progesterone metabolism in subcellular fractions, 839
%?EIItOXDI
899
TABLE OF CONTENTS - VOLUME 24 Steroid Radioimmunoassay: Contribution of Standards to Blank Values. MARIJKE FROLICH, W. TERMORSHUIZEN, ELLY KENTER and A. J. MOOLENAAR . . . . . . . . . , . , .
1
The Effects of Synthetic Estrogens on the Metabolic Clearance and Production Rates of Estrone and Estradiol. C. LONGCOPE, D. WATSON and K.I.H. WILLIAMS. . . . . . . .
15
Determination of Progesterone and of Free and Conjugated Estrogens in Pregnant and Pseudo-Pregnant Rats. S. DE LAUZON, F. UHRICH, S. VANDEL, N. CITTANOVA and M.F.JAYLE.................... . . t
31
The Pituitary-Gonadal Axis Before Puberty: Effects of Various Estrogenic Steroids in the Ovariectomized Rat. J. C. MCPHERSON III, J. C. ELDRIDGE, A. COSTOFF and V.B.MAHESH.......................
41
Estradiol-178-Hydroxysteroid Dehydrogenase Activity in Preimplantation Rat Embryos. SUDHANSU K. DEY and Z.DICKMANN.. . . . . . . . . . . . . . . . . . . ..a
57
Synthesis and Antiestrogenic Activity of 2,4-BisfBromoethyl)-Estrone Methyl Ether: A Bifunctional Alkylating Steroid. NA~SIMHA RAO. ~NAMARLAPUDI, FREDERICK SWEET and JAMES C. WARREN . . _ . , , . . . . . . , . . . . . ,
63
Intramolecular Cyclisation Reactions of fig-Cholan24-Amide, 3a-Acetoxy-Ss-Cholan-24-Amide, and 3a-Acetoxy-Se-Cholan-24-01, P. HODGE, G. M. PERRY and M. POLLARD. . A . . . . . . . . . . , . . . . . . . .
79
Radioimmunoassay
W. A. COLBURN . . . . .
95
Studies on the Site of Origin of the Androgen Binding Protein Present in Epididymal Cytosol from Mature Intact Rabbits. BENJ~IN J. DANZO, BARBARA C. ELLER and MARIE-CLAIRE ORGEBIN-CRIST. . . . . . . . . , . . . .
107
Androgen Binding Protein (ABP) in Rabbit Testis and Epididymis. S. C. WEDDINGTON, W. S. MCLEAN, S. N. NAYFEH, F. S. FRENCH, V. HANSSON and E. M. RITZEN .
123
A Simple Preparation of Z-Methyl-5a-Cholest-Z-enc. M. ADINOLFI, M, PARRILLI, G. BARONE and G, LAONIGRO , . .
135
Errata...
137
for Prednisone.
. . . . . . . . . . . . . . . . . . . . . . .
900
S
TDEOXDI
Testosterone Formation in Testis of the Innnature Androgen Insensitive Pseudohermaphrodite Male Rat (Stanley-Gumbreck Rat). P. A. ARONIN, J. C. COFFEY, F. S. FRENCH, and S. N. NAYFEH. . . . . . . . . . . . . .
139
Steroid Biosynthesis by Reptilian Adrenals. A. Z. MEHDI and T. SANDOR . . . . . . . . . . . . . . . .
151
Nutritional Effect of Possible Intermediates of Phytosterol Dealkylation in the Silkworm, ~ombyz mori. MASUO MORISAKI, HIROSHI OHOTAKA, NOR10 AWATA, NOBUO IKEKAWA, YASUHIRO HORIE, and SHOICHI NAKASONE . . .
165
Isolation of ACTH-Induced Protein from Adrenal Perfusate. S. G. LAYCHOCK and R. P. RUBIN. . . . . . . .
177
New Estetrols in Pregnancy Urine. N. F. TAYLOR and C.H.L. SHACKLETON . . . . . . . . . . . . . . . . . . . .
185
Plasma Testosterone During Nocturnal Sleep in Normal Men. RAUL C. SCHIAVI, DAVID M. DAVIS, DANIEL WHITE, ADELE EDWARDS, GEORGE IGEL, and CHARLES FISHER. . . . . .
191
Brisbagenin and Brisbenone, Two New Spirostanes from Species. K. JEWERS, M. B. BURBAGE, G. BLUNDEN, and W. J. GRIFFIN . . . . . . . . . . . . . .
203
The Effect of Antiestrogens on the Nuclear Binding of the Estrogen Receptor. THOMAS S. RUH and MARYF.RUH.......................
209
Estriol in Pregnancy. III. Development, Comparison and Use of Specific Antisera for Rapid Radioimmunoassay of Unconjugated Estriol in Pregnancy Plasma. HIDEHIKO KATAGIRI, FRANK Z. STANCZYK, and UWE GOEBELSMANN . . . . . . . . . . . . . . . . . . . . .
225
Bile Acids XLII. Preparation of 5a-Cholestan-26-oic Acids from Kryptogenin. ME1 ME1 MUI, S. Y. KAMAT, and WILLIAM H. ELLIOTT. . . . . . . . . . . . . . . . . .
239
Plasma Cortisol Concentrations in Two Teleost Fishes, auratus L.: A anguilla L. and Carassius Comparison of Two Assay Systems. GORDON HARGREAVES and JACQUELINE PORTHE-NIBELLE . . . . . . . . . . . . . .
251
Acetylation and Hydroxylation of 5a-Androstane-3s,17sdiol by Prostate and Epididymis. PETER OFNER and RICHARD L. VENA, and ROBERT F. MORFIN . . . . . . . . . .
261
CordyZine
Anguilla
S
901
-EDEOJDrn
Effects of Prior Androgen Exposure upon Adrenal Function in Maturing Rats. JUDITH A.RAMALEY. . . . . . . . . . . . Plasma Androgens in Idiopathic Hirsutism. and V.H.T. JAMES . . . . . . . . . . . .
281
C.M. ANDRE
. . . . . . . . . 295
Conversion of Testosterone-4-14C to Androgens of the 5a-Androstane Series by a Normal Human Ovary. O.W. SMITH, P. OFNER and R.L. VENA . . . . . . . . . . . .
311
A Non-Chromatographic Radioimnunoassay for Serum Dehydroepiandrosterone Using a Mixture of Antisera. HISAHIKO SEKIHARA and NAKAAKI OHSAWA . . . . . . . . . . .
317
A Sephadex Column Radioimmunoassay of Unconjugated Estriol in Pregnancy Plasma. JAMES E. CHRISTNER and MARION C. FETTER . . . . . . . . . . . . . . . . . . . . .
327
Facile Synthesis of Deuterated Estrogens.
. .
343
Conversion of 20a-Hydroxy-4-Pregnen-3-One to 20a-Hydroxy-5a-Pregnan-3-One and 5a-Pregnane-3a,20a-Diol by Rat Anterior Pituitary. FELICIA V. NOWAK and HARRY J. KARAVOLAS . . . . . . . . . . . . . . . . . . . .
351
3-Epi-20-Hydroxyecdysone from Meconium of the Tobacco Hornworm. MALCOLM J. THOMPSON, JOHNN. KAPLANIS, WILLIAME. ROBBINS,SAMSON R. DUTKY and HERBERT N. NIGG..
359
In vitro
R.C. MURPHY
Synthesis of 24,28-Iminofucosterol and Its Inhibitory Effects on Growth and Steroid Metabolism in the Silkworm,
Bombyx mori.
YOSHINORI FUJIMOTO, MASUOMORISAKI,
NOBUO IKEKAWA, YASUHIRO HORIE and SHOICHI NAKASONE . . . .
367
A Novel Bufadienolide Synthesis. A. BELANGER, P. BRASSARD, G. DIONNE and CH. R. ENGEL. . . . . . . . . .
377
245-Methylcholestane-38,5a,66,25_Tetrol 25-Monoacetate A Novel Polyhydroxylated Steroid from an Alcyonarian. J. MICHAEL MOLDOWAN, BERNARD M. TURSCH and CARL DJERASSI .
387
Marine Sterols. V. Isolation and Structure of Occelasterol, a New 27-Norergostane-type Sterol, from MASARU KOBAYASHI an Anne1 ida, Peeudopotamilla occeZata. and HIROSHI MITSUHASHI . . . . . . . . . . . . . . . . .
. 399
Identification by Gas Chromatography-Mass Spectrometry of Intermediates in the Aromatization of Modified Clg Steroids by Human Placental Microsomes. W. EMMETT BRASELTON, JR., JAMES C. ORR and LEWIS L. ENGEL.
411
Errata..........................
435
902
S
TElEOIDl
Two High Affinity Estrogen Binding Proteins of Different Specificity in the Immature Rat Uterus Cytosol. GEORGES MICHEL, INGRID JUNG, ETIENNE-EMILE BAULIEU, CLAUDE AUSSEL and JOSE URIEL . . . . . . . . . . . . . . .
437
Regulation of Aldosterone Synthesis. A. PEYTREMANN, R. D. BROWN, W. E. NICHOLSON, D. P. ISLAND, G. W. LIDDLE and J. G. HARDMAN. . . . . . . . . . . . . . . . . . . . .
451
The Synthesis and Biological Activity of 25,26Dihydroxy-Cholecalciferol, a Polar Metabolite of Vitamin D3. J. REDEL, P. A. BELL, N. BAZELY, Y. CALANDO, F. DELBARRE and E. KODICEK . . . . . . . . . .
463
Antiserum to 5a-Ditiydrotestosterone: Production, Characterization and Use in Radioimmunoassay. S. BAUMINGER, F. KOHEN, H. R. LINDNER and A. WEINSTEIN
477
. .
The Influence of Enzymes on the Estrogen Receptors of Human Uterus and Breast Carcinoma. ROLAND HAHNEL, ELLA TWADDLE and LARAINE BRINDLE . . . . . . . . . . . . .
489
Tissue Androgens and Androphilic Proteins in Rat Epididymis During Sexual Development. R. S. CALANDRA, E. J. PODESTA, M. A. RIVAROLA and J. A. BLAQUIER . . . . .
507
In vitro Metabolism of Estrone, 2,4,6,7-3H and 4-Androstene-3,17-Dione-1,2-3H in Submandibular Gland and Submandibular Gland Cancer Tumor. TAWFIK M. A. ElATTAR . . . . . . . . . . . . . . , . . . .
519
Interaction of Estradiol and ACTH in the Regulation of Adrenal Corticosterone Production in the Rat. HOWARD D. COLBY and JULIAN I. KITAY. . . . . . . . . .
527
The Uptake and Metabolism of Labelled Testosterone by the Brain and Pituitary of the Male Rhesus Monkey (Macaca rmdatta). LEON J. SHOLITON, BRANDON B. TAYLOR and H. PAUL LEWIS. . . . . . . . . . . . . . . . . . .
I
.
. .
537
Desmosteryl Sulfate and Desmosterol in Hamster Epididymal Spermatozoa. G. BLEAU and W. J. A. VANDEN HEUVEL . . . . . . . . . . . . . . . . . .
549
Biliary Metabolites of Testosterone and Testosterone Glucosiduronate in the Rat. MICHIO MATSUI, YUKO KINUYAMA and MISAKO HAKOZAKI. . . . . . . . . . . . .
557
Regional Differences in Androgen Thresholds of the Epididymis of the Castrated Rat. GAYATRI GUPTA, M. RAJALAKSHMI and M. R. N. PRASAD . . . . . . . . . . . .
575
S
-EmEOIDI
903
A Direct Determination of Plasma Aldosterone. MARIE T. PHAM-HUU-TRUNG and PIERRE CORVOL . . . . . . . . . .
587
Receptor Progesterone Complex in the Nuclear Fraction of the Rat Uterus: Demonstration by 3H-Progesterone Exchange. A.J.W. HSUEH, E. J. PECK, JR., and J. H. CLARK. . . . . . . .
599
A New Class of Long-Acting Hormonal Steroid Pr'eparation: Synthesis of Dimeric Ethynodiol and Nortestosterone, of Dimeric and Trimeric Androgens and of some Dimeric Combinations of Steroids. HERBERT KUHL and HANS-DIETER TAUBERT . . . . . . . . . . . . . . . . . . . , .
613
A Formal Synthesis of 18-Hydroxyestrone. D. K. BANERJEE, S. D. VENKATARAMU, and P. K. SEN. . . . . . . . . . . . . . .
627
Trace Analysis of Ecdysones by Gas-Liquid Chromatography, Radioimmunoassay and Bioassay. DAVID W. BORST and JOHN D. O'CONNOR. . . . . . . . . . . . . . . . . . . . . . .
637
Temporal Changes in Serum Estradiol and Prolactin in Immature Female Rats Given a Single Injection of Estradiol Benzoate. H. C. CHENG and D. C. JOHNSON. . . . . .
657
A Radioimmunoassay of Plasma Estradiol-176 with the Use of Polyethylene Glycol to Separate Free and Antibody-bound HARVEY S. SCHILLER and MARGARET A. BRAMMALL . . . . Hormone.
665
Effects of Anterior Pituitary Hormones on Hepatic Corticosterone Metabolism in Rats. HOWARD D. COLBY, JOE H. GASKIN, and JULIAN I. KITAY. . . . . . . . . . . . . .
679
4-Hydroxyestrone: A New Metabolite of Estradiol-178 from Humans. J. G. WILLIAMS, C. LONGCOPE, and K.I.H. WILLIAMS . . . . . . . . . . . . . . . . . . . . . . .
687
Ontogeny of Mitochondrial Steroidogenesis in the Guinea S. A. SHOLL . . . . , . . . . . . . . . . . . . . Pig Gonad.
703
The Steroidogenic Ability of Various Cell Types of the Equine Ovary. DAMODAR K. MAHAJAN and LEO T. SAMUELS. . , . .
713
The Prolonged Progestational and Anti-estrogenic Activity of 6,118-Dichloro-3B,17-Dihydroxy-19-nor-4,6-Pregnadien20-One 17-Acetate 3B-Benzoate (GR2/2132). H. G. GILBERT, E. A. WOOLLETT, and K. J. CHILD . . . . . . . . . . . . . . .
731
3-Methoxy-1,3,5(10)-estratriene-17(R)-spiro-6'-{2'-[bis(2-chloroethyl)amino]-2'-0~0-3'oxa-3'-aza-2'-phosphorane). EMERSON L. FOSTER and ROBERT T. BLICKENSTAFF. . . . . . . , .
737
904
S
T-EOIDI
Synthesis of Corticosteroid Haptens. AK10 TSUJI, MILLIE SMULOWTIZ, JULIA S. C. LIANG and DAVID K. FUKUSHIMA .
739
The Conversion of Testosterone to 7a-Hydroxy-Testosterone by Incubated Rat Testes. W. EECHAUTE, E. LACROIX and I.LEUSEN..........................
753
Estrogen Induction of 20a-Hydroxysteroid Dehydrogenase in Mouse Vaginal Tissue. KARL C. PODRATZ, THEODORE W. MUNNS and PHILIP A. KATZMAN. . . . . . . . . . . . . . . . . . . .
765
Metabolism of Progesterone in Mouse Vaginal Tissue. KARL C. PODRATZ, THEODORE W. MUNNS and PHILIP A. KATZMAN . .
775
Specific Antisera Suitable for Solid-Phase Radioimmunoassay of llg-Hydroxyandrost-4-ene-3,17-dione. P. NARASIMHA RAO, PERRY H. MOORE, JR. and J. W. GOLDZIEHER . . . , . . . . . .
793
Antisera for Radioimmunoassay of 17a-Ethynylestradiol and Mestranol. P. NARASIMHA RAO, ARMANDO DE LA PENA and JOSEPH W. GOLDZIEHER . . . . . . . . . . . . . . . . . . . .
803
Simultaneous Measurement of Unbound Testosterone and Estradiol Fractions in Undiluted Plasma at 37" C by Steady-State Gel Filtration. R. A. FISHER, D. C. ANDERSON and C. W. BURKE . . . . . . . . . . . . . . .
809
A Study on the Stability of an Estrogen-Protein Conjugate Conditions. Y. Y. TSONG and S.S.KOIDE.........................
825
Metabolism of Progesterone in Subcellular Fractions of Rat Uterus. JUDITH SAFFRAN, BONNIE K. LOESER, BERNADETTE i4. HAAS and HOMER E. STAVELY. . . . . . . . . . .
839
The Effect of Oral Contraceptive Steroid Therapy on the Urinary Metabolites of Radioactive Estrone and Estradiol-17B. ANDREA M. FEMINO, CHRISTOPHER LONGCOPE, JUNE G. WILLIAMS and KENNETH I. H. WILLIAMS. . . . . . . . .
849
Site of Conjugation of Bovine Serum Albumin to Corticosteroid Hormones and Specificity of Antibodies. TOSHIHIRO NISHINA, AK10 TSUJI and DAVID K. FUKUSHIMA . . . .
861
Studies on the Metabolism of Steroid Hormones. PART II. Reversible Steroid-Protein Interactions via Enol Intermediates. PETER TOFT . . . . . . . . . . . . . . . . .
875
Effects of Fetectomy on Plasma Estrogens and Progesterone in Monkeys (Xacaca mulatta). WILLIAM W. TULLNER and GARY D. HODGEN . . . . . . . . . . . . . . . . . . . . . . .
887
Volume 24 Table of Contents, Author
899
in vivo and Under in vitro
Index, Subject Index . .
S
TDEOIDI
AUTHOR INDEX - VOLUME 24 Adinolfi, M., 135 Anderson, D.C., 809 Andre, C. M., 295 Aronin, P. A., 139 Aussel, C., 437 Awata, Norio, 165 Banerjee, D. K., 627 Barone, G., 135 Baulieu, E.-E., 437 Bauminger, S., 477 Basely, N., 463 Belanger, A., 377 Bell, P. A., 463 Blaquier, J. A., 507 Bleau, G., 549 Blickenstaff, R. T., 737 Blunden, G., 203 Borst, David, 637 Brammall, M. A., 665 Braselton, W. E., Jr., 411 Brassard, P., 377 Brindle, Laraine, 489 Brown, R. D., 451 Burbage, M. B., 203 Burke, C. W., 809 Calando, Y., 463 Calandra, R. S., 507 Cheng, H. C., 657 Child, K. J., 731 Christner, J. E., 327 Cittanova, N., 31 Clark, J. H., 599 Coffey, J. C., 139 Colburn, W. A., 95 Colby, H. D., 527, 679 Corvol, P., 587 Costoff, A., 41 Danzo, B. J., 107 David M., 191 de la Pena, Armando, 803 de Lauzon, S., 31 Delbarre, F., 463 Dey, S. K., 57 Dickmann, Z., 57 Dionne, G., 377 Djerassi, C., 387 Dutky, Samson R., 359 Davis,
Edwards, Adele, 191 Eechaute, W., 753 El Attar, Tawfik M.A., 519 Eldridge, J., 41 Eller, B. C., 107 Elliott, W. H., 239 Engel, Ch. R., 377 Engel, L. L., 411 Femino, A. M., 849 Fetter, M. C., 327 Fisher, Charles, 191 Fisher, R. A., 809 Foster, E. L., 737 French, F. S., 123, 139 Frolich, Marijke, 1 Fujimoto, Yoshinori, 367 Fukushima, David K., 739, 861 Gaskin, Joe H., 679 Gupta, Gayatri, 575 Gilbert, H. G., 731 Goebelsmann, U., 225 Goldzieher, J. W., 793, 803 Griffin, W. J., 203 Haas, B. M., 839 Hahnel, Roland, 489 Hakozaki, M., 557 Hansson, V., 123 Hardman, J. G., 451 Hargreaves, Gordon, 251 Hodge, P., 79 Hodgen, G. D., 887 Horie, Yasuhiro, 165, 367 Hsueh, A. J. W., 599 Igel, George, 191 Ikekawa, Nobuo, 165, 367 Island, D. P., 451 James, V. H. T., 295 Jayle, M. F., 31 Jewers, K., 203 Johnson, D. C., 657 Jung, I., 437
905
Kamat, s. Y., 239 Kanamarlapudi, N., 63 Kaplanis, John N, 359 Karavolas, H. J., 351 Katagiri, H., 225 Katzman, Philip A., 765, 775 Kenter, Elly, 1 Kinuyama, Y., 557 Kitay, J. I., 527, 679 Kobayashi, M., 399 Kodicek, E., 463 Kohen, F., 477 Koide, S. S., 825 Kuhl, H., 613 Lacroix, E., 753 Laonigro, G., 135 Laychock, S. G., 177 Leusen, I., 753 Lewis, H. Paul, 537 Liang, Julia S., 739 Liddle, G. W., 451 Lindner, H. R., 477 Loeser, B. K., 839 Longcope, C., 15, 687, 849 Mahajan, D. K., 713 Mahesh, Virendra, B., 41 Matsui, M., 557 Mehdi, A. Z., 151 Michel, G., 437 Mitsuhashi, H., 399 Moldowan, J. M., 387 Moolenaar, A. J., 1 Moore, Perry H., Jr., 793 Morfin, R. F., 261 Morisaki, Masuo, 165, 367 Mui, M. M., 239 Munns, Theodore W., 765, 775 Murphy, R. C., 343 McLean, W. S., 123 McPherson, J. C., III, 41 Nakasone, Shoichi, 165, 367 Nayfeh, S. N., 123, 139 Nicholson, W. E., 451 Nigg, Herbert N., 359 Nishina, Toshihiro, 861 Nowak, F. V., 351
O'Connor, John D., 637 Ofner, Peter, 261, 311 Ohotaka, Hiroshi, 165 Ohsawa, N., 317 Orgebin-Crist, M.-C., 107 Orr, J. C., 411 Parrilli, M., 135 Peck, E. J., Jr., 599 Perry, G. M., 79 Peytremann, A., 451 Pham-Huu-Trung, M. T., 587 Podesta, E. J., SO7 Podratz, Karl C., 765, 775 Pollard, M., 79 Porthe-Nibelle, Jacqueline, 251 Prasad, M. R. N., 575 Rajalakshmi, M., 575 Ramaley, Judith A., 281 Rao, P. Narasimha, 793, 803 Redel, J., 463 Ritzen, E. M., 123 Rivarola, M. A., 507 Robbins, William E., 359 Rubin, R. P., 177 Ruh, Mary F., 209 Ruh, Thomas S., 209 Saffran, J., 839 Samuels, L. T., 713 Sandor, T., 151 Schiavi, Raul C., 191 Schiller, H. S., 665 Sekihara, H., 317 Sen, P. K., 627 Shackleton, C. H. L., 185 Sholiton, Leon J., 537 Sholl, S. A., 703 Smith, Olive W., 311 Smulowitz, M., 739 Stavely, H. E., 839 Stanczyk, F. Z., 225 Sweet, Frederick, 63
S Taubert, H.-D., 613 Taylor, Brandon B., 537 Taylor, N. F., 185 Termorshuizen, W., 1 Thompson, Malcolm J., 359 Toft, P., 875 Tsong, Y. Y., 825 Tsuji, Akio, 739, 861 Tullner, W. W., 887 Tursch, B. M., 387 Twaddle, Ella, 489 Uhrich, F., 31 Uriel, J., 437
7?-EOXDI
Vandel, S., 31 VandenHeuvel, W. J. A., 549 Vena, Richard L., 261, 311 Venkataramu, S. D., 627 Warren, James C., 63 Watson, D., 15 Weddington, S. C., 123 Weinstein, A., 477 White, Daniel, 191 Williams, J. G., 687, 849 Williams, K. I. H., 15, 687, 849 Woollett, E. A., 731
907
SUBJECT INDEX - VOLUME 24 3a-Acetoxy-5B-cholan-24-amide, intramolecular cyclisation of, 79 ACTH, induced protein synthesis and release, 177 Adrenal Corticosterone production, in rat, 527 Adrenal protein release, 177 Alcyonarian, natural products from, 387 Aldosterone, biosynthesis of, 451 direct radioimmunoassay without chromatography, 587 Androgen binding proteins, in rabbits, 107 Androgenization, early, in rats, 281 Androgens, in rat tissues, 507 plasma levels in hirsutism, 295 Androphilic proteins, in rat epididymis, 507 So-Androsta-3S,17B-dial. metabolism of, 261 5a-Androstan-17B-ol-3-one, antiserum to and radioimmunoassay of in human plasma, 477 binding protein of, 107 -la-carboxy thioether, synthesis of, 477 product of testosterone-4- 14C incubated with normal human ovary, 311 regional differences of in the castrated rat, 575 Androst-4-en-68,11fi-diol-3,17-dione, 6-hemisuccinate-BSA, synthesis of, 793 Androstendione, in cancer tumor, 519 in hirsutism, 295 highly specific antisera of, 793 Androst-4-en-ll@ol-3,17-dione, Androst-4-en-17S-ol-3-one, dimeric, 613 regional differences in thresholds of the epididymis of the castrated rat, 575 Antiestrogen activity, 63 Antiestrogens, in vitro inhibition of nuclear estrogen receptor binding, 209 Antisera, for estriol radioimmunoassay, 225 Aromatization, of Clg intermediates, 411 Bioavailability, of prednisone, 95 Biosynthesis, of steroids in mare ovaries, 713 2,4-bis(bromomethyl)-3-methoxy-1,3,5[10]-trien-17-one, antiestrogenic activity of, 63 Bovine adrenal cells, regulation of aldosterone synthesis, 451 Brain, monkey, the uptake and metabolism of labelled testosterone in, 537 Bufadienolides, novel synthesis of, 377 Cardiotonic steroids, of the bufadienolide type, contribution to the synthesis of, 377 58-Cholana-3a,24-diol-3a-acetoxy, intramolecular cyclisation of, 79 5B-Cholan-24-amide, intramolecular cyclisation of, 79 5a-Cholesta-3a,7a-diol-26-carboxylic acid, preparation of, 239 Cholest-5-en-3$,26-diol-16,22-dione, preparation of, 239
S
TBEOIDl
Cholest-5-en-38-01,side chain cleavage of, 703 Competitive protein binding assay (CPBA), cortisol level in teleostei, a justification by comparison with DIDA, 253 Contraceptive steroids, effect on estrogen metabolism, 849 Corticosteroid haptens, synthesis of, 739 Corticosterone metabolism, in rats, 679 Corticosterone production, in rat adrenal, 527 Cyclophosphamide, steroid derivative of, 737 Dealkylation, of phytosterol in insect, 165 Dealkylation inhibitor, in silkworm, 367 Desmosteryl sulfate, in hamster spermatozoa, 549 6, 118-Dichloro-38,17-dihydroxy-l9-nor-4,6-pregnadien-2O-one17-acetate-38-benzoate,prolonged activity of, 731 25,26-Dihydroxycholecalciferol,biological activity and synthesis of, 463 38,7a- and 3a,7a-Dihydroxy-5a-cholestan-26-oic acids, preparation of, 239 18,38-Dihydroxy-5a-25a-spirostane(brisbagenin), conversion to 3$-hydroxy-5a-25a-spirostane(tigogenin)via 5a,25a-spirost-1-en-3-one(brisbenone),203 isolation and characterisation from CordyZine cannifolia, 203 8-Ecdysone, radioimmunoassay of, 637 Ecdysones, from Meconium of the Tobacco Hornworm, 359 Epididymal androgen receptor, in rabbits, 123 Epididymis, androgen binding protein in, 107 Clg-steroid acetylation and hydroxylation of, 261 Regional differences in androgen thresholds in the castrated rat, 575 3-Epi-20-hydroxyecdysone,from Meconium of the Tobacco Hornworm, 359 24,28-Epoxystigmast-5-en-38-01,synthesis of, 367 Estetrols, in pregnancy urine, 185 Estradiol, interaction of in rat adrenal, 527 measurement of unbound fraction in plasma by steady-state gel filtration, 809 metabolism of, 849 Estradiol-dz,synthesisof, 343 Estradiol-178, metabolism of, 687 radioimmunoassay of, 665 serum levels after estradiol benzoate injection, 657 4-3H, preparation and metabolism of, 687 hydroxysteroid dehydrogenase, in morulae and blastocysts, 57 [3HlEstradiol-178-succinyl,in bovine serum albumin, 825 Estradiol binding protein, in rat, 437 Estradiol receptor, 437 Estra-1,3,5(10)-trien-3,16a,l78-triol, radioimmunoassay of, 225 radioimmunoassay of unconjugated in human pregnancy plasma, 327 Estriol-d2,synthesisof, 343
909
Estrogen, induction of 20a-hydroxysteroid dehydrogenase, 765 metabolism of, 15, 775 production of, 15 -protein, conjugate of, 825 receptor, in rat, 437 receptors, in human uterus and breast carcinoma, 489 Estrogens, effect of on serum gonadotropins, 41 in pregnancy urine, 185 in rat plasma, 31 Estrone, in cancer tumor, 519 metabolism of, 849 -d,, synthesis of, 343 17a-Ethinyl-4-estrene-36,17@diol, dimeric, 613 Ethynylestradiol, effect on estrogen metabolism, 849 17a-Ethynylestra-1,3,5(10)-trien-3,17@biol, antisera of, 803 17a-Ethynyl-3-methoxyestra-l,3,5(lO)-trien-17B-o1, antisera of, 803 Fetal gonad, steroidogenesis in, 703 Fetectomy, effects of on plasma steroids, 887 al-Fetoprotein, in rat, 437 Fucosterol-24,28-epoxide, 165 Gas chromatography-mass spectrometry, identification of Cl9 intermediates, 411 of steroids, 185 Gonadotropins (FSH and LH), effect of estrogens on, 41 Guinea pig, steroidogenesis in, 703 2B,38,14a,2OR,22R,25-Hexahydroxy-cholest-7-en-6-one (p-ecdysone), 637 Hirsutism, plasma androgen levels in, 295 unbound fractions of testosterone and estradiol in plasma, 809 Hormones, from Meconium of the Tobacco Hornworm, 359 Hydrolytic enzymes, influence on estrogen receptors and estradiol-receptor complex, 489 2-Hydroxyestradiol, as a metabolite of estradiol, 687 as metabolite of estrone and estradiol, 849 18-Hydroxyestriol, in pregnancy urine, 185 4-Hydroxyestrone, as a metabolite of estradiol, 687 18-Hydroxyestrone, synthesis of, 627 7o-Hydroxy-testosterone, formation of in testes, 753 Hydroxylation, of testosterone, 875 2Oa-Hydroxysteroid dehydrogenase, in mouse vagina, 765 24,28-Iminostigmast-5-en-3B-ol,
synthesis of, 367
Marine sterols, polyhydroxylated, 387 24-Methylcholestane derivative, from alcyonarian, 387 24S-Methylcholesta-3B,5a,6B,25-tetraol-25-acetoxy, 387
2-Methyl-5a-cholest-2-ene, preparation of from 5a-cholestan-38-01, Monkeys, plasma progesterone and estrogens after fetectomy, 887
135
27-nor-(24S)-24-Methylcholesta-5,22-dien-3B-o1, from an annelida, 399 Nuclear receptor, progesterone complex in, 599 Nutritional effect in insect of sterol derivative, 165 Oligomeres, steroid, 613 Ontogeny, of receptor, in rat, 437 Ovary, normal human, presence of A4-3-oxosteroid 5a-reductase in, 311 steroid biosynthesis in, 713 Oxaazaphosphorane, 17-Spiro, 737 Pituitary, anterior in rat, metabolism of pregn-4-en-20a-ol-3-one in, 351 monkey, the uptake and metabolism of labelled testosterone in, 537 response of to increased serum estrogen in immature female rats, 657 Pituitary hormones, effect of on corticosterone metabolism, 679 Placenta, human, Cl9 intermediates in, 411 Polyhydroxylated steroid, from alcyonarian, 387 Polyethylene glycol, preciptation of steroid antibodies with, 665 Prednisone, radioimmunoassay of, 95 5a-Pregnan-2Ca-ol-3-one, formation from 20a-DHP in anterior pituitary in rat, 351 Pregn-4-en-20a-ol-3-one, anterior pituitary metabolism of in rat, 351 Pregn-4-en-llB,17a,2l-triol-3,2O-dione, determination and comparison of level by CPBA and DIDA, 253 Progesterone, in rat plasma, 31 metabolism of by pseudohermaphrodite rat testes, 139 metabolism, in mouse vagina, 775 metabolism of in subcellular fractions of uterus, 839 receptor in rat uterus, 599 Progesterone-3H, exchange assay of, 599 Prolactin, release of in response to estradiol, 657 Prostate, C 19-steroid acetylation and hydroxylation of, 261 Puberty, adrenal function in rats, 281 a-Pyrones, synthesis of, 377 Rabbit, TeBG in, 123 Radioimmunoassay, blank values in paper chromatography, 1 for serum dehydroepiandrosterone without chromatography, 317 of Androst-4-en-llB,ol-3,17-dione, 793 of corticosteroids, 861 of estriol, 225 of plasma estradiol, 665 of prednisone, 95 of unconjugated estriol in human pregnancy plasma, 327 Rats, pregnant and pseudo-pregnant, determination of progesterone and estrogens in, 31 Rat uterus, mechanism of action of some antiestrogens in, 209 Reptilian adrenal, steroidogenesis, 151
Sephadex (G-lo), in column radioimmunoassay of unconjugated estriol in human pregnancy plasma, 327 Sleep, testosterone level in plasma during, 191 5a,25a-Spirost-1-en-3-one (brisbenone), isolation and characterisation from CordyZine sp, 203 Steroid, bifunctional alkylating, 63 Teleostei, cortisol levels in eel and goldfish plasma, 253 Testes, metabolization of testosterone in, 753 Testicular androgen binding protein (ABP), in rabbits, 123 Testosterone, formation of in pseudohermaphrodite rat testes, 139 hydroxylation of, 875 in plasma during sleep, 191 measurement of unbound fraction in plasma by steady-state gel filtration, 809 metabolization of in testes, 753 metabolization, in the brain and pituitary of the male Rhesus monkey, 537 Testosterone-17-glucosiduronate, metabolism of in the rat, 557 Uterus, estrogen receptor in, in rat, 437 progesterone metabolism in subcellular fractions, 839