- Email: [email protected]
The biology spectrometer for small-angle X-ray scattering at the national synchrotron light source
202
Nuclear Instruments and Methods in Physics Research 222 (1984) 202 North-Holland, Amsterdam
THE BIOLOGY SPECTROMETER SYNCHROTRON
LIGHT SOURCE
FOR SMALL-ANGLE
X-RAY SCATTERING
AT THE NATIONAL
*
D a v i d S. W I S E a n d B e n n o P. S C H O E N B O R N
Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
A small-angle X-ray scattering (SAXS) spectrometer for structural biology has been installed at the National Synchrotron Light Source. This instrument will be used to study a diverse number of biology samples (e.g., membranes, muscle, protein or DNA complexes, viruses) and has been designed, accordingly, to achieve high small-angle resolution (5000 ,~) and high angular resolution (0.1 mrad). The spectrometer incorporates automated motions for vertical and horizontal beam tracking, and raster scan calibration of the detector, in addition to sample and detector positioning axes. The sample-to-detectordistance can be adjusted between 50 and 300 cm, and the 2/9 axis between - 5 and 45 °. To provide unobstructed space below and to one side of the sample, the detector mounting arm is catilevered from an offset 28 axis. The sample mounting position is designed so that a number of different sample-containing devices may be used. Presently, a three-circle goniometer, membrane sample cell and multiple-capillary sample changer are available; additional devices including stopped-flow and T-jump cells are planned. Each spectrometer axis is equipped with a stepping motor and absolute position encoder. A general purpose motor control unit [1], connected by an asynchronous serial link to the experiment control computer, translates function commands from the processor into TTL pulse trains output to the selected motor's power supply and reports each axis' status and position back to the processor.
Reference [1] F.W. Stubblefield and J.L. Alberi, IEEE Trans. Nucl. Sci. NS-36 (1983) 240.
* Work supported by the US Department of Energy. 0167-5087/84/$03.00 © Elsevier Science Publishers B.V. ( N o r t h - H o l l a n d Physics Publishing Division)
Nuclear Instruments and Methods in Physics Research 222 (1984) 202 North-Holland, Amsterdam
THE BIOLOGY SPECTROMETER SYNCHROTRON
LIGHT SOURCE
FOR SMALL-ANGLE
X-RAY SCATTERING
AT THE NATIONAL
*
D a v i d S. W I S E a n d B e n n o P. S C H O E N B O R N
Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
A small-angle X-ray scattering (SAXS) spectrometer for structural biology has been installed at the National Synchrotron Light Source. This instrument will be used to study a diverse number of biology samples (e.g., membranes, muscle, protein or DNA complexes, viruses) and has been designed, accordingly, to achieve high small-angle resolution (5000 ,~) and high angular resolution (0.1 mrad). The spectrometer incorporates automated motions for vertical and horizontal beam tracking, and raster scan calibration of the detector, in addition to sample and detector positioning axes. The sample-to-detectordistance can be adjusted between 50 and 300 cm, and the 2/9 axis between - 5 and 45 °. To provide unobstructed space below and to one side of the sample, the detector mounting arm is catilevered from an offset 28 axis. The sample mounting position is designed so that a number of different sample-containing devices may be used. Presently, a three-circle goniometer, membrane sample cell and multiple-capillary sample changer are available; additional devices including stopped-flow and T-jump cells are planned. Each spectrometer axis is equipped with a stepping motor and absolute position encoder. A general purpose motor control unit [1], connected by an asynchronous serial link to the experiment control computer, translates function commands from the processor into TTL pulse trains output to the selected motor's power supply and reports each axis' status and position back to the processor.
Reference [1] F.W. Stubblefield and J.L. Alberi, IEEE Trans. Nucl. Sci. NS-36 (1983) 240.
* Work supported by the US Department of Energy. 0167-5087/84/$03.00 © Elsevier Science Publishers B.V. ( N o r t h - H o l l a n d Physics Publishing Division)