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THE EFFECT OF HELICOBACTER PYLORI ERADICATION ON SERUM LIPOPROTEIN AND HIGH SENSITIVE CRP LEVELS
Poster Sessions PO25 Inflammatory markers PO25-407
THE EFFECT OF HELICOBACTER PYLORI ERADICATION ON SERUM LIPOPROTEIN AND HIGH SENSITIVE CRP LEVELS
M. Durakoglugil 1 , U. Arslan 1 , A. Balcioglu 1 , M. Erden 1 , G. Erkan 2 , M. Cindoruk 2 , B. Boyaci 1 , A. Cengel 1 . 1 Department of Cardiology, Gazi University, Ankara, Turkey; 2 Department of Gastroenterology, Gazi University, Ankara, Turkey
Our study showed that CagA status does not seem to be a virulence marker for lipid profile changes and inflammation as thought. Eradication of helicobacter pylori may provide extraintestinal benefits such as decreased atherosclerosis risk by increasing apolipoprotein AI levels.
ical inflammatory response. The risk of carotid atherosclerosis associated with cigarette smoking was more pronounced in those with high CRP levels (>0.5mg/dl). Notably, the highest IMT occurred in the subgroup with combined smoking and elevated CRP levels (1.1±0.11 mm), whereas IMT in the smokers subgroup with low CRP levels (0.75±0.20 mm) was comparable to that in nonsmokers. Conclusions: In apparently healthy men, cigarette smoking elicits a subclinical inflammatory response in certain subjects. This inflammatory response appears to constitute a link in the association between cigarette smoking and atherosclerosis. PO25-409
F.J. del Canizo-Gomez 1 , M.N. Moreira-Andres 2 . 1 Endocrinology, Hospital Virgen de la Torre, Madrid, Spain; 2 Endocrinology, Hospital Universitario 12 de Octubre, Madrid, Spain Bakground: Elevated levels of highly sensitive C-reactive protein (hsCRP), were found in type 2 diabetes mellitus(T2DM) patients with metabolic syndrome(MS). Aims: To examine the relationship between hs-CRP levels and the MS components, in a population with an enhanced frequency of obesity as T2DM patients. Methods: Four hundred and eighty eight T2DM subjects were recruited. Patients with hs-CRP≥ 10 mg/l, creatinine≥ 1.4 mg/dl, chronic inflammatory and cardiovascular diseases, smokers or on antiplatelet therapy were excluded. In all the 178 subjects, hs-CRP, waist circumference (WC), fasting plasma glucose, systolic and diastolic blood pressure, HDL-Cholesterol, and triglycerides were measured, and body mass index (BMI) calculated. The MS was defined according to the IDF criteria. Chi square test was used for analysis of frequency, Student’s t-test to compare the means, ANCOVA to assess the relationship between hs-CRP and the MS, Pearson correlation coefficients to assess the correlations of hs-CRP with anthropometric variables, and multiple regression analysis to examine the relationship between hs-CRP>3 mg/l and MS components. A P-value <0.05 was considered significant (SPSS). Results: Women had higher frequency of abdominal obesity (AO) (P=0.000). Higher hs-CRP values were observed in those with AO (P=0.017), and the highest hs-CRP levels were observed in those MS subjects with AO (2.83±0.32 mg/l). Levels of hs-CRP correlated with WC (r=0.357, P=0.000) and BMI (r=0.341, P=0.000). AO (OR=5.01, P=0.035) and BMI≥30 (OR=4.1, P=0.001) were the most important MS components associated with hs-CRP levels>3 mg/l. Conclusions: WC and BMI are associated with hs-CRP levels, suggesting potential roles of AO and obesity in the development of the inflammation associated with the MS in T2DM patients. PO25-410
PO25-408
CIGARETTE SMOKING AND EARLY ATHEROSCLEROSIS IN HEALTHY YOUNG ADULTS: ROLE OF C-REACTIVE PROTEIN
H. Waly 1 , M. Abul-Ata 2 , N.S. Omar 3 . 1 Cardiology, Mansoura University Faculty of Medicine, Mansoura, Egypt; 2 Radiolgy, Mansoura University Faculty of Medicine, Mansoura, Egypt; 3 Immunology, Mansoura University Faculty of Medicine, Mansoura, Egypt Background: Although cigarette smoking is known to be associated with atherosclerosis, the results concerning progression of intima-media thickness (IMT) in smokers are conflicting. Objectives. We hypothesized that cigarette smoking elicits an inflammatory response, reflected by elevated C-reactive protein (CRP) levels, and predisposes to atherosclerosis, in part, through smoke-induced inflammation. Methods: IMT of the carotid artery was determined, using highresolution Duplex ultrasound, in 20 apparently healthy cigarette smoker men (mean age, 30±4.5 years) and 15 age-and -sex-matched life-long nonsmokers (mean age, 29±3 years). Blood samples from all studied subjects were tested for CRP levels. Results: IMT was significantly increased in smokers than in nonsmokers (0.94±0.28 vs. 0.56±0.14 mm; p<0.01). Moreover, smokers were significantly associated with increased serum CRP levels (0.45±0.86 vs. 0.24±0.73 mg/dl; p<0.01), suggesting that smoking could evoke a subclin-
C-REACTIVE PROTEIN IN OBESE PATIENTS WITH TYPE 2 DIABETES MELLITUS AND METABOLIC SYNDROME
PLATELET, ENDOTHELIAL AND MONOCYTE MICROPARTICLES ARE GENERATED DURING ACUTE MYOCARDIAL INFARCTION AND IN STABLE ANGINA
E. Stankiewicz 1 , E. Stepien 1 , A. Undas 2 , J. Zalewski 1 , J. Godlewski 1 , K. Zmudka 2 . 1 the John Paul II Hospital, Kraków; 2 Institute of Cardiology, Jagiellonian University School of Medicine, Kraków Background: Increased total number of microparticles (MP), accompanied by platelet activation and enhanced inflammation were reported in patients with myocardial infarction (AMI) and stable angina (SA). Materials and Methods: Microparticle counts were determined in 12 patients with documented ST-elevation AMI, 10 SA patients and 9 healthy controls. MP were assayed by flow cytometry. Platelet activation was determined by the expression of surface and soluble P-selectin, along with monocyteand neutrophil-platelet aggregates. Interleukin 6 (IL-6) and CD40 ligand (CD40L) levels were measured by ELISAs. Results: Patients with AMI displayed higher levels of all microparticles than patients with SA, however the only statistical difference was observed in CD31 MP levels (p=0.03). In patients with AMI were significant correlations between both the total number as well as the level of CD34, CD51, CD142, CD42 MP and expression of surface P-selectin. Conclusions: We conclude that in AMI, platelet activation is associated with increased generation of MPs from not only platelets, but also monocytes and endothelial cells. It suggests that interactions between platelets,
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey
POSTER SESSIONS
The fact that Helicobacter pylori infection is associated with modified lipid profile, especially lower HDL-cholesterol levels, is widely known. It is reasonable that CagA positive strains with increased inflammation would further deteriorate lipid composition. Therefore we conducted a study to evaluate the effect of helicobacter pylori eradication on serum lipoproteins and hsCRP and to reveal whether it is related with CagA status. To our knowledge this is the first study investigating this matter. 41 patients with positive rapid urease test were enrolled in the study. Serum lipoproteins, lipoprotein(a), hsCRP and CagA were analyzed at the beginning and 3 months after eradication therapy. According to urea breath test, patients with successful eradication formed Group I and those with unsuccessful eradication formed Group II. Lipoprotein and hsCRP levels did not change significantly in Group II and in Group I, except Apolipoprotein AI levels, which increased 7.8 mg/dl after treatment (p=0,003). Contrary to the expected, CagA negative strains were associated with increased Apolipoprotein AI levels when CagA status was taken into account.
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THE EFFECT OF HELICOBACTER PYLORI ERADICATION ON SERUM LIPOPROTEIN AND HIGH SENSITIVE CRP LEVELS
M. Durakoglugil 1 , U. Arslan 1 , A. Balcioglu 1 , M. Erden 1 , G. Erkan 2 , M. Cindoruk 2 , B. Boyaci 1 , A. Cengel 1 . 1 Department of Cardiology, Gazi University, Ankara, Turkey; 2 Department of Gastroenterology, Gazi University, Ankara, Turkey
Our study showed that CagA status does not seem to be a virulence marker for lipid profile changes and inflammation as thought. Eradication of helicobacter pylori may provide extraintestinal benefits such as decreased atherosclerosis risk by increasing apolipoprotein AI levels.
ical inflammatory response. The risk of carotid atherosclerosis associated with cigarette smoking was more pronounced in those with high CRP levels (>0.5mg/dl). Notably, the highest IMT occurred in the subgroup with combined smoking and elevated CRP levels (1.1±0.11 mm), whereas IMT in the smokers subgroup with low CRP levels (0.75±0.20 mm) was comparable to that in nonsmokers. Conclusions: In apparently healthy men, cigarette smoking elicits a subclinical inflammatory response in certain subjects. This inflammatory response appears to constitute a link in the association between cigarette smoking and atherosclerosis. PO25-409
F.J. del Canizo-Gomez 1 , M.N. Moreira-Andres 2 . 1 Endocrinology, Hospital Virgen de la Torre, Madrid, Spain; 2 Endocrinology, Hospital Universitario 12 de Octubre, Madrid, Spain Bakground: Elevated levels of highly sensitive C-reactive protein (hsCRP), were found in type 2 diabetes mellitus(T2DM) patients with metabolic syndrome(MS). Aims: To examine the relationship between hs-CRP levels and the MS components, in a population with an enhanced frequency of obesity as T2DM patients. Methods: Four hundred and eighty eight T2DM subjects were recruited. Patients with hs-CRP≥ 10 mg/l, creatinine≥ 1.4 mg/dl, chronic inflammatory and cardiovascular diseases, smokers or on antiplatelet therapy were excluded. In all the 178 subjects, hs-CRP, waist circumference (WC), fasting plasma glucose, systolic and diastolic blood pressure, HDL-Cholesterol, and triglycerides were measured, and body mass index (BMI) calculated. The MS was defined according to the IDF criteria. Chi square test was used for analysis of frequency, Student’s t-test to compare the means, ANCOVA to assess the relationship between hs-CRP and the MS, Pearson correlation coefficients to assess the correlations of hs-CRP with anthropometric variables, and multiple regression analysis to examine the relationship between hs-CRP>3 mg/l and MS components. A P-value <0.05 was considered significant (SPSS). Results: Women had higher frequency of abdominal obesity (AO) (P=0.000). Higher hs-CRP values were observed in those with AO (P=0.017), and the highest hs-CRP levels were observed in those MS subjects with AO (2.83±0.32 mg/l). Levels of hs-CRP correlated with WC (r=0.357, P=0.000) and BMI (r=0.341, P=0.000). AO (OR=5.01, P=0.035) and BMI≥30 (OR=4.1, P=0.001) were the most important MS components associated with hs-CRP levels>3 mg/l. Conclusions: WC and BMI are associated with hs-CRP levels, suggesting potential roles of AO and obesity in the development of the inflammation associated with the MS in T2DM patients. PO25-410
PO25-408
CIGARETTE SMOKING AND EARLY ATHEROSCLEROSIS IN HEALTHY YOUNG ADULTS: ROLE OF C-REACTIVE PROTEIN
H. Waly 1 , M. Abul-Ata 2 , N.S. Omar 3 . 1 Cardiology, Mansoura University Faculty of Medicine, Mansoura, Egypt; 2 Radiolgy, Mansoura University Faculty of Medicine, Mansoura, Egypt; 3 Immunology, Mansoura University Faculty of Medicine, Mansoura, Egypt Background: Although cigarette smoking is known to be associated with atherosclerosis, the results concerning progression of intima-media thickness (IMT) in smokers are conflicting. Objectives. We hypothesized that cigarette smoking elicits an inflammatory response, reflected by elevated C-reactive protein (CRP) levels, and predisposes to atherosclerosis, in part, through smoke-induced inflammation. Methods: IMT of the carotid artery was determined, using highresolution Duplex ultrasound, in 20 apparently healthy cigarette smoker men (mean age, 30±4.5 years) and 15 age-and -sex-matched life-long nonsmokers (mean age, 29±3 years). Blood samples from all studied subjects were tested for CRP levels. Results: IMT was significantly increased in smokers than in nonsmokers (0.94±0.28 vs. 0.56±0.14 mm; p<0.01). Moreover, smokers were significantly associated with increased serum CRP levels (0.45±0.86 vs. 0.24±0.73 mg/dl; p<0.01), suggesting that smoking could evoke a subclin-
C-REACTIVE PROTEIN IN OBESE PATIENTS WITH TYPE 2 DIABETES MELLITUS AND METABOLIC SYNDROME
PLATELET, ENDOTHELIAL AND MONOCYTE MICROPARTICLES ARE GENERATED DURING ACUTE MYOCARDIAL INFARCTION AND IN STABLE ANGINA
E. Stankiewicz 1 , E. Stepien 1 , A. Undas 2 , J. Zalewski 1 , J. Godlewski 1 , K. Zmudka 2 . 1 the John Paul II Hospital, Kraków; 2 Institute of Cardiology, Jagiellonian University School of Medicine, Kraków Background: Increased total number of microparticles (MP), accompanied by platelet activation and enhanced inflammation were reported in patients with myocardial infarction (AMI) and stable angina (SA). Materials and Methods: Microparticle counts were determined in 12 patients with documented ST-elevation AMI, 10 SA patients and 9 healthy controls. MP were assayed by flow cytometry. Platelet activation was determined by the expression of surface and soluble P-selectin, along with monocyteand neutrophil-platelet aggregates. Interleukin 6 (IL-6) and CD40 ligand (CD40L) levels were measured by ELISAs. Results: Patients with AMI displayed higher levels of all microparticles than patients with SA, however the only statistical difference was observed in CD31 MP levels (p=0.03). In patients with AMI were significant correlations between both the total number as well as the level of CD34, CD51, CD142, CD42 MP and expression of surface P-selectin. Conclusions: We conclude that in AMI, platelet activation is associated with increased generation of MPs from not only platelets, but also monocytes and endothelial cells. It suggests that interactions between platelets,
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey
POSTER SESSIONS
The fact that Helicobacter pylori infection is associated with modified lipid profile, especially lower HDL-cholesterol levels, is widely known. It is reasonable that CagA positive strains with increased inflammation would further deteriorate lipid composition. Therefore we conducted a study to evaluate the effect of helicobacter pylori eradication on serum lipoproteins and hsCRP and to reveal whether it is related with CagA status. To our knowledge this is the first study investigating this matter. 41 patients with positive rapid urease test were enrolled in the study. Serum lipoproteins, lipoprotein(a), hsCRP and CagA were analyzed at the beginning and 3 months after eradication therapy. According to urea breath test, patients with successful eradication formed Group I and those with unsuccessful eradication formed Group II. Lipoprotein and hsCRP levels did not change significantly in Group II and in Group I, except Apolipoprotein AI levels, which increased 7.8 mg/dl after treatment (p=0,003). Contrary to the expected, CagA negative strains were associated with increased Apolipoprotein AI levels when CagA status was taken into account.
117