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The Effect Of Human Placental Extract In a Mouse Model Of Allergic Rhinitis
AB144 Abstracts
505
SUNDAY
Interaction Between Dietary Antioxidants and Passive Smoking On The Risk Of Asthma Modified By GSTP1(rs1695) Polymorphism Dr. Young-Hwa Song, MD, MPH, Candidate1, Hye Lim Shin2, Seo Ah Hong3, Sung-Ok Kwon4, Dr. Young Ho Jung, MD5, Dr. Eun Lee, MD6, Dr. Song I. Yang, MD7, Dr. Ji-Won Kwon, MD8, Dr. Hyung Young Kim, MD9, Dr. Ju-Hee Seo, MD10, Dr. Byoung-Ju Kim, MD, PhD11, Dr. Hyo-Bin Kim, MD, PhD12, Se-Young Oh4, Dr. Soo-Jong Hong, MD, PhD7, Dr. Bong-Seong Kim, MD13; 1Johns Hopkins Bloomberg School of Public Health, 2Research Center for Standardization of Allergic Diseases, Asan Institute for Life Sciences, University of ulsan College of Medicine, 3Research Center for Standardization of Allergic Diseases, Asan Institute for Life Sciences, University of ulsan College of Medicine, South Korea, 4Department of Food and Nutrition, College of Human Ecology, Kyung Hee University, 5Childhood Asthma Atopy Center, Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, South Korea, 6Department of Pediatrics, Childhood Asthma Atopy Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea, 7Department of Pediatrics, Childhood Asthma Atopy Center, Research Center for Standization of Allergic Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, 8Department of Pediatrics, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, South Korea, 9Department of Pediatrics, Kosin University Gospel Hospital, Kosin University College of Medicine, Seoul, South Korea, 10Department of Pediatrics, Korea Cancer Center Hospital, 11 Department of Pediatrics, Hae-undae Paik Hospital, Inje University College of Medicine, Busan, South Korea, 12Department of Pediatrics, Inje University Sanggye Paik Hospital, Seoul, South Korea, 13Department of Pediatrics, Gangneung Asan Hospital, University of Ulsan College of Medicine. RATIONALE: Deficiencies of dietary antioxidants and exposure to smoking have been implicated in the etiology of lung and other cancers. This study investigated relationship between intake of antioxidant vitamin A, C and E, carotene, retinol and passive smoking on the risk of asthma. METHODS: Children aged 6-13 years in Seoul were surveyed in 2008 and 1,129 children were analyzed. A Korean version of ISAAC questionnaire and food frequency questionnaire (FFQ) were completed by their parents. GSTP1(rs1695) polymorphism was genotyped by the TaqMan assay. RESULTS: Passive smoking increased the risk of wheezing in the past 12 months (aOR, 2.48; 95% CI, 1.29-4.76) and asthma diagnosis (aOR, 1.91; 95% CI, 1.19-3.06). We found no relationship between antioxidants and wheezing in the past 12 months and asthma diagnosis. However, in combined analyses with two risk factors, there were interactions between passive smoking and lower intake of vitamin A on the risk of wheezing in the past 12 months (aOR, 4.43; 95% CI, 1.51-12.96; p for trend 0.0018) and asthma diagnosis (aOR, 2.23; 95% CI, 1.10-4.54; p for trend 0.0046). The relationship was more apparent with AA at GSTP1 rs1695 who had been exposed to two risk factors at increased risk of wheezing in the past 12 months (aOR56.66, 95% CI51.54-28.85) and asthma diagnosis (aOR54.44, 95% CI51.58-12.52) compared to those with AG or GG at this position and had not been exposed to two risk factors. CONCLUSIONS: Asthma may be modified by Gene-EnvironmentEnvironment interaction between GSTP1 AA polymorphism and dietary antioxidant/passive smoking.
506
Longitudinal Trends Of Food and Environmental AllergenSpecific IgE In Asthmatic Inner-City Children < 4 Years Of Age Dr. Keshav Achar, MBBS1, Yikania Pichardo2, Margaret A. Chin, NP3, Dr. Ramin Nazari, MD4, Dr. Andrew A. Wiznia, MD4, Dr. Gabriele De Vos, MD5; 1Albert Einstein College of Medicine, NY, 2Jacobi Medical Center, New York, NY, 3Jacobi Medical Center, NY, 4Albert Einstein College of Medicine, 5Albert Einstein College of Medicine, Bronx, NY. RATIONALE: The most dynamic changes in allergen- specific IgE happen in early childhood. Yet, little is known about the longitudinal trends
J ALLERGY CLIN IMMUNOL FEBRUARY 2014
of allergen-specific IgE in atopic, inner-city children with asthma aged < 4 years. METHODS: Preliminary data from children participating as controls in an asthma interventional trial was analyzed. Seventeen atopic inner-city children aged < 4 years with recurrent wheeze, parental history of asthma and/or eczema were included. Specific serum IgE to 7 common food and 8 common environmental allergens was obtained at baseline and then yearly _0.35kU/l. after up to 3 years. We defined sensitization as IgE > RESULTS: At baseline, 16 children (median age 33 months; IQR 26, 46 months) were sensitized to at least 1 food and 1 environmental allergen. Six children (35%) lost at least one food sensitization and 14 (82%) had a decrease of specific food IgE over time. In contrast, only 2 (12%) developed new food sensitizations and 10 (59%) had an increase of one or more food specific IgE. Regarding environmental allergens, 2 (12%) lost at least one of their environmental sensitizations and 12 (71%) had a decrease of environmental IgE. Three (18%) patients developed new sensitizations and 12 (71%) showed an increase in one or more environmental IgEs. CONCLUSIONS: Inner-city children with asthma are unlikely to outgrow sensitizations to environmental allergens and commonly show increase in specific IgE to environmental allergens. Even though they are more likely to outgrow food allergies, a considerable number of children may retain or show increase in food sensitizations.
507
The Effect Of Human Placental Extract In a Mouse Model Of Allergic Rhinitis Dr. Soo Whan Kim, Dr. Suk-Woo Yang; The Catholic University of Korea. RATIONALE: Effect of placental extract to regulate biological responses and its potential as therapeutic reagents in various diseases have been implicated in numerous studies. In this study, we investigate preventive and therapeutic effects of placental extract in a mouse model of allergic rhinitis. METHODS: BALB/c mice were divided into control, Derf, Pre-S and Pre-C groups. The allergen was Dermatophagoides farinae (Derf). HPE was administered before sensitization (Pre-S) or before challenge (PreC). Allergic symptom scores, eosinophil counts and serum Derf-specific IgE levels were measured. Interferone-g, T-bet, GATA-3, IL-4 and Foxp3 mRNA expression in nasal mucosa were determined by real-time polymerase chain reaction. And Interferone-g, T-bet, GATA-3, IL-4 were confirmed by western-blotting analysis. Flow cytometry of CD4+CD25+Foxp3+ T cells in spleen were analyzed. RESULTS: Symptom scores, serum Derf-specific IgE, GATA-3 mRNA levels, IL-4 mRNA levels and tissue eosinophil counts were decreased in both Pre-S and Pre-C groups (all, p<0.05). Quantitation of western blots showed that expression of GATA-3 and IL-4 were decreased in both PreS and Pre-C groups than Derf group. Also, percentage of CD4+CD25+Foxp3+ T cells, Foxp3 mRNA levels were increased in PreS and Pre-C groups compared with those in Derf group (all, p<0.05). CONCLUSIONS: Both prophylactic and therapeutic treatment with HPE significantly reduces allergic airway inflammation and has potential for induction regulatory T cells in a murine model of allergic rhinitis.
505
SUNDAY
Interaction Between Dietary Antioxidants and Passive Smoking On The Risk Of Asthma Modified By GSTP1(rs1695) Polymorphism Dr. Young-Hwa Song, MD, MPH, Candidate1, Hye Lim Shin2, Seo Ah Hong3, Sung-Ok Kwon4, Dr. Young Ho Jung, MD5, Dr. Eun Lee, MD6, Dr. Song I. Yang, MD7, Dr. Ji-Won Kwon, MD8, Dr. Hyung Young Kim, MD9, Dr. Ju-Hee Seo, MD10, Dr. Byoung-Ju Kim, MD, PhD11, Dr. Hyo-Bin Kim, MD, PhD12, Se-Young Oh4, Dr. Soo-Jong Hong, MD, PhD7, Dr. Bong-Seong Kim, MD13; 1Johns Hopkins Bloomberg School of Public Health, 2Research Center for Standardization of Allergic Diseases, Asan Institute for Life Sciences, University of ulsan College of Medicine, 3Research Center for Standardization of Allergic Diseases, Asan Institute for Life Sciences, University of ulsan College of Medicine, South Korea, 4Department of Food and Nutrition, College of Human Ecology, Kyung Hee University, 5Childhood Asthma Atopy Center, Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, South Korea, 6Department of Pediatrics, Childhood Asthma Atopy Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea, 7Department of Pediatrics, Childhood Asthma Atopy Center, Research Center for Standization of Allergic Diseases, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, 8Department of Pediatrics, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, South Korea, 9Department of Pediatrics, Kosin University Gospel Hospital, Kosin University College of Medicine, Seoul, South Korea, 10Department of Pediatrics, Korea Cancer Center Hospital, 11 Department of Pediatrics, Hae-undae Paik Hospital, Inje University College of Medicine, Busan, South Korea, 12Department of Pediatrics, Inje University Sanggye Paik Hospital, Seoul, South Korea, 13Department of Pediatrics, Gangneung Asan Hospital, University of Ulsan College of Medicine. RATIONALE: Deficiencies of dietary antioxidants and exposure to smoking have been implicated in the etiology of lung and other cancers. This study investigated relationship between intake of antioxidant vitamin A, C and E, carotene, retinol and passive smoking on the risk of asthma. METHODS: Children aged 6-13 years in Seoul were surveyed in 2008 and 1,129 children were analyzed. A Korean version of ISAAC questionnaire and food frequency questionnaire (FFQ) were completed by their parents. GSTP1(rs1695) polymorphism was genotyped by the TaqMan assay. RESULTS: Passive smoking increased the risk of wheezing in the past 12 months (aOR, 2.48; 95% CI, 1.29-4.76) and asthma diagnosis (aOR, 1.91; 95% CI, 1.19-3.06). We found no relationship between antioxidants and wheezing in the past 12 months and asthma diagnosis. However, in combined analyses with two risk factors, there were interactions between passive smoking and lower intake of vitamin A on the risk of wheezing in the past 12 months (aOR, 4.43; 95% CI, 1.51-12.96; p for trend 0.0018) and asthma diagnosis (aOR, 2.23; 95% CI, 1.10-4.54; p for trend 0.0046). The relationship was more apparent with AA at GSTP1 rs1695 who had been exposed to two risk factors at increased risk of wheezing in the past 12 months (aOR56.66, 95% CI51.54-28.85) and asthma diagnosis (aOR54.44, 95% CI51.58-12.52) compared to those with AG or GG at this position and had not been exposed to two risk factors. CONCLUSIONS: Asthma may be modified by Gene-EnvironmentEnvironment interaction between GSTP1 AA polymorphism and dietary antioxidant/passive smoking.
506
Longitudinal Trends Of Food and Environmental AllergenSpecific IgE In Asthmatic Inner-City Children < 4 Years Of Age Dr. Keshav Achar, MBBS1, Yikania Pichardo2, Margaret A. Chin, NP3, Dr. Ramin Nazari, MD4, Dr. Andrew A. Wiznia, MD4, Dr. Gabriele De Vos, MD5; 1Albert Einstein College of Medicine, NY, 2Jacobi Medical Center, New York, NY, 3Jacobi Medical Center, NY, 4Albert Einstein College of Medicine, 5Albert Einstein College of Medicine, Bronx, NY. RATIONALE: The most dynamic changes in allergen- specific IgE happen in early childhood. Yet, little is known about the longitudinal trends
J ALLERGY CLIN IMMUNOL FEBRUARY 2014
of allergen-specific IgE in atopic, inner-city children with asthma aged < 4 years. METHODS: Preliminary data from children participating as controls in an asthma interventional trial was analyzed. Seventeen atopic inner-city children aged < 4 years with recurrent wheeze, parental history of asthma and/or eczema were included. Specific serum IgE to 7 common food and 8 common environmental allergens was obtained at baseline and then yearly _0.35kU/l. after up to 3 years. We defined sensitization as IgE > RESULTS: At baseline, 16 children (median age 33 months; IQR 26, 46 months) were sensitized to at least 1 food and 1 environmental allergen. Six children (35%) lost at least one food sensitization and 14 (82%) had a decrease of specific food IgE over time. In contrast, only 2 (12%) developed new food sensitizations and 10 (59%) had an increase of one or more food specific IgE. Regarding environmental allergens, 2 (12%) lost at least one of their environmental sensitizations and 12 (71%) had a decrease of environmental IgE. Three (18%) patients developed new sensitizations and 12 (71%) showed an increase in one or more environmental IgEs. CONCLUSIONS: Inner-city children with asthma are unlikely to outgrow sensitizations to environmental allergens and commonly show increase in specific IgE to environmental allergens. Even though they are more likely to outgrow food allergies, a considerable number of children may retain or show increase in food sensitizations.
507
The Effect Of Human Placental Extract In a Mouse Model Of Allergic Rhinitis Dr. Soo Whan Kim, Dr. Suk-Woo Yang; The Catholic University of Korea. RATIONALE: Effect of placental extract to regulate biological responses and its potential as therapeutic reagents in various diseases have been implicated in numerous studies. In this study, we investigate preventive and therapeutic effects of placental extract in a mouse model of allergic rhinitis. METHODS: BALB/c mice were divided into control, Derf, Pre-S and Pre-C groups. The allergen was Dermatophagoides farinae (Derf). HPE was administered before sensitization (Pre-S) or before challenge (PreC). Allergic symptom scores, eosinophil counts and serum Derf-specific IgE levels were measured. Interferone-g, T-bet, GATA-3, IL-4 and Foxp3 mRNA expression in nasal mucosa were determined by real-time polymerase chain reaction. And Interferone-g, T-bet, GATA-3, IL-4 were confirmed by western-blotting analysis. Flow cytometry of CD4+CD25+Foxp3+ T cells in spleen were analyzed. RESULTS: Symptom scores, serum Derf-specific IgE, GATA-3 mRNA levels, IL-4 mRNA levels and tissue eosinophil counts were decreased in both Pre-S and Pre-C groups (all, p<0.05). Quantitation of western blots showed that expression of GATA-3 and IL-4 were decreased in both PreS and Pre-C groups than Derf group. Also, percentage of CD4+CD25+Foxp3+ T cells, Foxp3 mRNA levels were increased in PreS and Pre-C groups compared with those in Derf group (all, p<0.05). CONCLUSIONS: Both prophylactic and therapeutic treatment with HPE significantly reduces allergic airway inflammation and has potential for induction regulatory T cells in a murine model of allergic rhinitis.