The effect of platelet-rich fibrin on bone regeneration and angiogenesis in rabbit cranial defects

E-Poster Presentation overexpressed in SCC. The expression of PCNA and CK were low in normal group, but sequentially increased in SCC. ALDH1, Nanog and OCT4 expression were significantly increased according to SCC grade during carcinogenesis. The findings indicate that CD133 may be useful in identifying oral CSCs, which suggests that CD133 may serve as a predictor to identify CSCs with a high risk of oral cancer development. http://dx.doi.org/10.1016/j.ijom.2015.08.184 MITEK® anchor system for TMJ disk plication: summary of 40 patients H. Kim ∗ , Y. Son, B. Lee Ulsan University, Department of Oral and Maxillofacial Surgery, Seoul Asan Medical Center, College of Medicine, Seoul, Republic of Korea Background: Chronic disk displacement of the temporomandibular joint (TMJ) is a major pathologic condition of temporomandibular joint disorders (TMD). Although conservative treatments such as physiotherapy, pharmacotherapy and splint therapy are necessary, but, in many such clinical conditions, those conventional approaches are not effective to solve the patient’s discomfort. For these patients, disk plication or disk repositioning methods might be beneficial to relieve the problems related to disk displacement. MITEK® anchorage system for disk plication surgery is a relatively recent technique and has several advantages when compared to conventional methods for disk plication. Objectives: The purpose of this study is to evaluate the clinical data from patients who were received disk plication surgery with MITEK® system and conclude that it might be useful and safe technique to repose the displaced disk of the TMJ. Methods: A total 40 patients (aged 15–69 years, mean 35.2 years) were included in this study who recently visited department of oral and maxillofacial surgery of Seoul Asan Medical Center and underwent disk plication surgery with MITEK® system. Magnetic resonance imaging (MRI) was taken in all patients. The patients were evaluated in aspects of TMJ noise, tenderness to palpation, mouth opening range, and complication. Findings and conclusions: The MITEK® mini anchor provides a predictable method for stabilizing the TMJ articular disc to the condyle. The improvement in postoperative pain, noise, and mouth opening range is significant. http://dx.doi.org/10.1016/j.ijom.2015.08.185 Changes in the angle of inferior turbinate after inferior turbinate outfracture in superior repositioning Le Fort I osteotomy H.G. Kim 1,∗ , Y.H. Kim 1,2 , J.C. Park 1,2 , M.S. Kang 1,2 , Y.H. Son 1,2 , J.Y. Yoon 2 , Y.D. Kim 1 1

Pusan National University, Department of Oral and Maxillofacial Surgery, Pusan, Republic of Korea 2 Pusan National University, Department of Dental Anesthesia and Pain Medicine, Pusan, Republic of Korea Background: The superior repositioning Le Fort I osteotomy in orthognathic surgery can affect nasal airway flow and nasal cavity volume. To compensate this problem, Bell described inferior turbinectomy that the amount of superior movement was over 5 mm to avoid impacting the nasal floor against the inferior turbinate in order to maintain the nasal function. But, these

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procedures can be lead to several complications, such as necrosis of the turbinate bone, eschar formation, atrophic rhinitis, empty nose syndrome, prolonged crusting, rhinitis sicca, epiphora, and postoperative bleeding. Objectives: We analyzed inferior turbinate outfracture to clarify it is safe, convenient, time-saving and less invasive technique and it can minimize complications. Methods: Orthognathic surgery was performed on 325 consecutive patients between the 14 months period of January 2011 and March 2012. There are 183 impaction cases and 45 vertical reduction cases. The amount of superior movement was more than 4 mm in 45 impaction cases and 27 vertical reduction cases. Among these, we investigated the amount of change in the angle of inferior turbinate of 20 persons using pre- and post-operative CBCT data and cephalogram. On the basis of results, we compared relapse rates and analyzed the difference between 2-dimensional and 3-dimensional results. Conclusions: The angle between the inferior turbinate bone and the lateral wall of the nasal cavity were observed to be reduced at post-operative follow-up and relapse rates were acceptable. Therefore these inferior turbinate outfracture is enough to improve and compensate for nasal volume decreases. http://dx.doi.org/10.1016/j.ijom.2015.08.186 The effect of platelet-rich fibrin on bone regeneration and angiogenesis in rabbit cranial defects J.K. Kim ∗ , H.J. Yoon, S.H. Lee The Catholic University of Korea, Department of Oral and Maxillofacial Surgery, Seoul St. Mary’s Hospital, College of Medicine, Seoul, Republic of Korea Objectives: This study evaluated the effect of platelet-rich fibrin on the osteogenesis and angiogenesis of rabbit calvarial defects. Methods: The calvarium of nine rabbits was exposed. In each rabbit, two circular bone defects were prepared. While the experimental sites received PRF, the control sites were left filled with blood. The animals were sacrificed after a period of 1 week (N = 3), 2 weeks (N = 3), 4 weeks (N = 3). The following histomorphometric data were recorded: (1) newly formed tissue relative to total bone defect (NT), newly formed osteoid relative to newly formed tissue (NB), (2) the longest and shortest vertical height of the newly formed tissue relative to the vertical height of normal tissue (LH, SH), (3) newly formed osteoid on the defect margin using region of interest (NBR), (4) bony pontic formation from the margin (OS). The expression of VEGF was evaluated by immunohistochemical staining. Results: The differences between groups were not significant at any experimental time point. However, a significant linear increase of NB and NBR including OS value according to time was observed in the PRF group. In addition, the intensity and percentage range of VEGF expression showed a significant linear decrease trend with the time in the PRF group. Conclusions: The results of this study suggest that PRF may enhance the quality of bone regeneration. http://dx.doi.org/10.1016/j.ijom.2015.08.187